(Circular) (Six-base) MAPSORT of: pFN21AE4548.seq Check: 8676 from: 1 to: 6646 pFN21AE4548 6646 bp With 182 enzymes: SgfI * March 28, 2012 14:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4456 278 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3989 1937 Size: 2052 4594 Acc65I G'GTAC_C Cuts at: 2086 2462 3967 2086 Size: 376 1505 4765 Fragments arranged by size: 4765 1505 376 AclI AA'CG_TT Cuts at: 1563 4773 5146 1563 Size: 3210 373 3063 Fragments arranged by size: 3210 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2478 2859 3009 3438 3765 4709 6093 6361 Size: 846 381 150 429 327 944 1384 268 Cuts at: 6361 1632 Size: 1917 Fragments arranged by size: 1917 1384 944 846 429 381 327 268 150 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6449 AflIII A'CryG_T Cuts at: 1203 5545 6522 1203 Size: 4342 977 1327 Fragments arranged by size: 4342 1327 977 AgeI A'CCGG_T Cuts at: 5477 5477 Size: 6646 AhdI GACnn_n'nnGTC Cuts at: 5375 5375 Size: 6646 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6646 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6646 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2179 2211 2179 Size: 32 6614 AlwNI CAG_nnn'CTG Cuts at: 3233 4389 5961 3233 Size: 1156 1572 3918 Fragments arranged by size: 3918 1572 1156 ApaLI G'TGCA_C Cuts at: 4703 5859 4703 Size: 1156 5490 ApoI r'AATT_y Cuts at: 1493 3423 3487 3674 3727 3955 4181 4333 6442 Size: 1930 64 187 53 228 226 152 2109 Cuts at: 6442 1493 Size: 1697 Fragments arranged by size: 2109 1930 1697 228 226 187 152 64 53 AseI AT'TA_AT Cuts at: 160 1032 3311 5200 160 Size: 872 2279 1889 1606 Fragments arranged by size: 2279 1889 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 AvaI C'yCGr_G Cuts at: 1870 1943 2057 3971 1870 Size: 73 114 1914 4545 Fragments arranged by size: 4545 1914 114 73 BamHI G'GATC_C Cuts at: 3976 4343 3976 Size: 367 6279 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2086 2321 2462 3241 3967 Size: 325 217 627 299 235 141 779 726 Cuts at: 3967 5422 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 779 726 627 325 299 235 217 141 BanII G_rGCy'C Cuts at: 1391 2032 3892 3965 1391 Size: 641 1860 73 4072 Fragments arranged by size: 4072 1860 641 73 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2258 3253 928 Size: 971 359 995 4321 Fragments arranged by size: 4321 995 971 359 BbvCI CC'TCA_GC Cuts at: 2554 2554 Size: 6646 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4837 4871 1903 Size: 34 2900 34 3678 Fragments arranged by size: 3678 2900 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4538 5748 4538 Size: 1210 5436 BclI T'GATC_A Cuts at: 1548 3859 1548 Size: 2311 4335 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2048 5257 136 Size: 107 122 71 631 605 376 3209 1525 Fragments arranged by size: 3209 1525 631 605 376 122 107 71 BglII A'GATC_T Cuts at: 6641 6641 Size: 6646 BlpI GC'TnA_GC Cuts at: 4063 4063 Size: 6646 Bme1580I G_kGCm'C Cuts at: 1790 2176 2705 4707 5863 1790 Size: 386 529 2002 1156 2573 Fragments arranged by size: 2573 2002 1156 529 386 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6646 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2187 3886 4377 Size: 416 53 83 399 24 770 1699 491 Cuts at: 4377 5335 442 Size: 958 1753 Fragments arranged by size: 1753 1699 958 770 491 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3834 1056 Size: 2778 3868 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2444 5306 1239 Size: 1205 2862 2579 Fragments arranged by size: 2862 2579 1205 Bpu10I CC'TnA_GC Cuts at: 2554 2628 2554 Size: 74 6572 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3738 4771 5636 5934 3738 Size: 1033 865 298 4450 Fragments arranged by size: 4450 1033 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5309 882 Size: 617 3810 2219 Fragments arranged by size: 3810 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4437 493 Size: 711 3233 2702 Fragments arranged by size: 3233 2702 711 BsaBI GATnn'nnATC Cuts at: 6640 6640 Size: 6646 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4453 4835 5470 275 Size: 53 83 186 936 2920 382 635 1451 Fragments arranged by size: 2920 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5079 5477 5751 5898 6309 5079 Size: 398 274 147 411 5416 Fragments arranged by size: 5416 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6421 Fragments arranged by size: 6421 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 1186 Size: 1255 5391 BseYI C'CCAG_C Cuts at: 2581 3003 3037 5849 6345 2581 Size: 422 34 2812 496 2882 Fragments arranged by size: 2882 2812 496 422 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6073 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2251 4096 4857 5885 1540 Size: 275 186 250 1845 761 1028 2301 Fragments arranged by size: 2301 1845 1028 761 275 250 186 BsiHKAI G_wGCw'C Cuts at: 4707 4792 5863 4707 Size: 85 1071 5490 Fragments arranged by size: 5490 1071 85 BsmI GAATG_Cn' Cuts at: 4157 4250 4157 Size: 93 6553 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2176 2705 3892 3965 4707 4792 Size: 399 242 144 529 1187 73 742 85 Cuts at: 4792 5863 1391 Size: 1071 2174 Fragments arranged by size: 2174 1187 1071 742 529 399 242 144 85 73 BspEI T'CCGG_A Cuts at: 6309 6309 Size: 6646 BspHI T'CATG_A Cuts at: 4535 6265 4535 Size: 1730 4916 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2653 4001 844 Size: 1809 1348 3489 Fragments arranged by size: 3489 1809 1348 BsrBI CCG'CTC Cuts at: 1394 1823 2132 4533 1394 Size: 429 309 2401 3507 Fragments arranged by size: 3507 2401 429 309 BsrDI GCAATG_nn' Cuts at: 65 1248 2288 3612 5141 5315 65 Size: 1183 1040 1324 1529 174 1396 Fragments arranged by size: 1529 1396 1324 1183 1040 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5290 5477 1700 Size: 110 3480 187 2869 Fragments arranged by size: 3480 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6646 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6646 BssSI C'ACGA_G Cuts at: 2100 4706 5718 2100 Size: 2606 1012 3028 Fragments arranged by size: 3028 2606 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3776 794 Size: 2982 3664 BstBI TT'CG_AA Cuts at: 5450 5450 Size: 6646 BstEII G'GTnAC_C Cuts at: 1184 2369 1184 Size: 1185 5461 BstXI CCAn_nnnn'nTGG Cuts at: 2698 2698 Size: 6646 BstYI r'GATC_y Cuts at: 1972 3976 4343 4728 4745 6197 6283 6295 6641 Size: 2004 367 385 17 1452 86 12 346 Cuts at: 6641 1972 Size: 1977 Fragments arranged by size: 2004 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2514 2514 Size: 6646 BtgI C'CryG_G Cuts at: 513 1065 2045 2210 2697 513 Size: 552 980 165 487 4462 Fragments arranged by size: 4462 980 552 487 165 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5907 Fragments arranged by size: 5907 683 56 BtsI GCAGTG_nn' Cuts at: 2601 4162 4956 4976 2601 Size: 1561 794 20 4271 Fragments arranged by size: 4271 1561 794 20 ClaI AT'CG_AT Cuts at: 1554 3354 1554 Size: 1800 4846 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6611 DraI TTT'AAA Cuts at: 2533 2946 3471 3950 2533 Size: 413 525 479 5229 Fragments arranged by size: 5229 525 479 413 DraIII CAC_nnn'GTG Cuts at: 3868 3868 Size: 6646 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5653 809 Size: 507 4337 1802 Fragments arranged by size: 4337 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2248 2982 4093 Size: 54 1425 50 275 18 418 734 1111 Cuts at: 4093 4982 6611 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1111 889 734 418 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2248 4093 1537 Size: 275 436 1845 4090 Fragments arranged by size: 4090 1845 436 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4576 1353 Size: 3223 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5233 5607 5753 387 Size: 1064 138 3644 374 146 1280 Fragments arranged by size: 3644 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2444 2478 2859 3009 3438 3765 4709 Size: 393 812 34 381 150 429 327 944 Cuts at: 4709 5306 6093 6361 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 944 812 787 597 429 393 381 327 268 150 34 EcoNI CCTnn'n_nnAGG Cuts at: 3225 3225 Size: 6646 EcoO109I rG'GnC_Cy Cuts at: 3230 3230 Size: 6646 EcoRI G'AATT_C Cuts at: 3674 3955 4333 3674 Size: 281 378 5987 Fragments arranged by size: 5987 378 281 EcoRV GAT'ATC Cuts at: 3169 3271 3169 Size: 102 6544 FspI TGC'GCA Cuts at: 5152 5152 Size: 6646 HaeII r_GCGC'y Cuts at: 1678 5793 1678 Size: 4115 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2475 2507 3158 3190 5287 Size: 32 135 32 903 32 651 32 2097 Cuts at: 5287 5319 5361 5393 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2097 903 651 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3990 4236 669 Size: 988 281 2052 246 3079 Fragments arranged by size: 3079 2052 988 281 246 HindIII A'AGCT_T Cuts at: 748 3450 748 Size: 2702 3944 HpaI GTT'AAC Cuts at: 4236 4236 Size: 6646 KpnI G_GTAC'C Cuts at: 2090 2466 3971 2090 Size: 376 1505 4765 Fragments arranged by size: 4765 1505 376 MfeI C'AATT_G Cuts at: 3771 4245 3771 Size: 474 6172 MluI A'CGCG_T Cuts at: 6522 6522 Size: 6646 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2389 3020 3865 5760 5944 1305 Size: 1084 631 845 1895 184 2007 Fragments arranged by size: 2007 1895 1084 845 631 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4824 Fragments arranged by size: 4824 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2696 4604 4963 5122 518 Size: 648 1530 1908 359 159 2042 Fragments arranged by size: 2042 1908 1530 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2212 2987 3212 4062 4739 5887 6132 1606 Size: 606 775 225 850 677 1148 245 2120 Fragments arranged by size: 2120 1148 850 775 677 606 245 225 NcoI C'CATG_G Cuts at: 513 1065 2045 2697 513 Size: 552 980 652 4462 Fragments arranged by size: 4462 980 652 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6646 NheI G'CTAG_C Cuts at: 1052 3830 1052 Size: 2778 3868 NotI GC'GGCC_GC Cuts at: 4093 4093 Size: 6646 NruI TCG'CGA Cuts at: 2113 2113 Size: 6646 NspI r_CATG'y Cuts at: 1132 1229 2662 3468 3762 4004 5549 1132 Size: 97 1433 806 294 242 1545 2229 Fragments arranged by size: 2229 1545 1433 806 294 242 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6646 PciI A'CATG_T Cuts at: 5545 5545 Size: 6646 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6646 PfoI T'CCnGG_A Cuts at: 3232 3232 Size: 6646 PmeI GTTT'AAAC Cuts at: 3950 3950 Size: 6646 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4715 4747 6228 6260 4715 Size: 32 1481 32 5101 Fragments arranged by size: 5101 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3230 3230 Size: 6646 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6646 PsiI TTA'TAA Cuts at: 3512 4216 3512 Size: 704 5942 PspXI vC'TCGA_Gb Cuts at: 2057 2057 Size: 6646 PstI C_TGCA'G Cuts at: 830 2232 3562 3805 3998 830 Size: 1402 1330 243 193 3478 Fragments arranged by size: 3478 1402 1330 243 193 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6646 PvuII CAG'CTG Cuts at: 3212 3212 Size: 6646 SacII CC_GC'GG Cuts at: 2213 2213 Size: 6646 SalI G'TCGA_C Cuts at: 1936 3988 1936 Size: 2052 4594 SbfI CC_TGCA'GG Cuts at: 3562 3998 3562 Size: 436 6210 ScaI AGT'ACT Cuts at: 3553 4894 6606 3553 Size: 1341 1712 3593 Fragments arranged by size: 3593 1712 1341 SfcI C'TryA_G Cuts at: 826 1045 2151 2228 2787 2920 3252 3558 3662 Size: 219 1106 77 559 133 332 306 104 Cuts at: 3662 3801 3994 5129 5810 6001 826 Size: 139 193 1135 681 191 1471 Fragments arranged by size: 1471 1135 1106 681 559 332 306 219 193 191 139 133 104 77 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6646 SmaI CCC'GGG Cuts at: 1872 3973 1872 Size: 2101 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2057 3753 4750 5651 5913 820 Size: 197 926 114 1696 997 901 262 1553 Fragments arranged by size: 1696 1553 997 926 901 262 197 114 SnaBI TAC'GTA Cuts at: 493 4437 493 Size: 3944 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6646 SphI G_CATG'C Cuts at: 1132 4004 1132 Size: 2872 3774 SspI AAT'ATT Cuts at: 5 52 4570 5 Size: 47 4518 2081 Fragments arranged by size: 4518 2081 47 StuI AGG'CCT Cuts at: 1517 2528 1517 Size: 1011 5635 StyI C'CwwG_G Cuts at: 513 1065 2045 2697 4085 513 Size: 552 980 652 1388 3074 Fragments arranged by size: 3074 1388 980 652 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2720 4673 4690 4843 5028 5473 325 Size: 1255 1140 1953 17 153 185 445 1498 Fragments arranged by size: 1953 1498 1255 1140 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2800 3551 4892 Size: 274 80 33 51 1443 823 751 1341 Cuts at: 4892 6604 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1341 823 751 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3660 3692 6606 6638 3660 Size: 32 2914 32 3668 Fragments arranged by size: 3668 2914 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6646 XbaI T'CTAG_A Cuts at: 3982 3982 Size: 6646 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2695 2695 Size: 6646 XhoI C'TCGA_G Cuts at: 1943 2057 1943 Size: 114 6532 XmaI C'CCGG_G Cuts at: 1870 3971 1870 Size: 2101 4545 XmnI GAAnn'nnTTC Cuts at: 4775 4775 Size: 6646 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4454 276 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspOMI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI