(Linear) (Six-base) MAPSORT of: KIEE4548.seq Check: 2463 from: 1 to: 1941 KIEE4548.orf With 182 enzymes: SgfI * March 22, 2012 18:20 .. Acc65I G'GTAC_C Cuts at: 0 81 457 1941 Size: 81 376 1484 Fragments arranged by size: 1484 376 81 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 473 854 1004 1433 1760 1941 Size: 473 381 150 429 327 181 Fragments arranged by size: 473 429 381 327 181 150 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 174 206 1941 Size: 174 32 1735 Fragments arranged by size: 1735 174 32 AlwNI CAG_nnn'CTG Cuts at: 0 1228 1941 Size: 1228 713 ApoI r'AATT_y Cuts at: 0 1418 1482 1669 1722 1941 Size: 1418 64 187 53 219 Fragments arranged by size: 1418 219 187 64 53 AseI AT'TA_AT Cuts at: 0 1306 1941 Size: 1306 635 AvaI C'yCGr_G Cuts at: 0 52 1941 Size: 52 1889 BanI G'GyrC_C Cuts at: 0 81 316 457 1236 1941 Size: 81 235 141 779 705 Fragments arranged by size: 779 705 235 141 81 BanII G_rGCy'C Cuts at: 0 27 1887 1941 Size: 27 1860 54 Fragments arranged by size: 1860 54 27 BbsI GAAGACnn'nnnn_ Cuts at: 0 253 1248 1941 Size: 253 995 693 Fragments arranged by size: 995 693 253 BbvCI CC'TCA_GC Cuts at: 0 549 1941 Size: 549 1392 BclI T'GATC_A Cuts at: 0 1854 1941 Size: 1854 87 BglI GCCn_nnn'nGGC Cuts at: 0 43 1941 Size: 43 1898 Bme1580I G_kGCm'C Cuts at: 0 171 700 1941 Size: 171 529 1241 Fragments arranged by size: 1241 529 171 BmrI ACTGGGnnnn_n' Cuts at: 0 182 1881 1941 Size: 182 1699 60 Fragments arranged by size: 1699 182 60 BmtI G_CTAG'C Cuts at: 0 1829 1941 Size: 1829 112 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 1941 Size: 439 1502 Bpu10I CC'TnA_GC Cuts at: 0 549 623 1941 Size: 549 74 1318 Fragments arranged by size: 1318 549 74 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1733 1941 Size: 1733 208 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 436 1941 Size: 436 1505 BseYI C'CCAG_C Cuts at: 0 576 998 1032 1941 Size: 576 422 34 909 Fragments arranged by size: 909 576 422 34 BsiEI CG_ry'CG Cuts at: 0 246 1941 Size: 246 1695 Bsp1286I G_dGCh'C Cuts at: 0 27 171 700 1887 1941 Size: 27 144 529 1187 54 Fragments arranged by size: 1187 529 144 54 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 648 1941 Size: 648 1293 BsrBI CCG'CTC Cuts at: 0 127 1941 Size: 127 1814 BsrDI GCAATG_nn' Cuts at: 0 283 1607 1941 Size: 283 1324 334 Fragments arranged by size: 1324 334 283 BssSI C'ACGA_G Cuts at: 0 95 1941 Size: 95 1846 BstAPI GCAn_nnn'nTGC Cuts at: 0 1771 1941 Size: 1771 170 BstEII G'GTnAC_C Cuts at: 0 364 1941 Size: 364 1577 BstXI CCAn_nnnn'nTGG Cuts at: 0 693 1941 Size: 693 1248 Bsu36I CC'TnA_GG Cuts at: 0 509 1941 Size: 509 1432 BtgI C'CryG_G Cuts at: 0 40 205 692 1941 Size: 40 165 487 1249 Fragments arranged by size: 1249 487 165 40 BtsI GCAGTG_nn' Cuts at: 0 596 1941 Size: 596 1345 ClaI AT'CG_AT Cuts at: 0 1349 1941 Size: 1349 592 DraI TTT'AAA Cuts at: 0 528 941 1466 1941 Size: 528 413 525 475 Fragments arranged by size: 528 525 475 413 DraIII CAC_nnn'GTG Cuts at: 0 1863 1941 Size: 1863 78 EaeI y'GGCC_r Cuts at: 0 243 977 1941 Size: 243 734 964 Fragments arranged by size: 964 734 243 EagI C'GGCC_G Cuts at: 0 243 1941 Size: 243 1698 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 473 854 1004 1433 1760 1941 Size: 439 34 381 150 429 327 181 Fragments arranged by size: 439 429 381 327 181 150 34 EcoNI CCTnn'n_nnAGG Cuts at: 0 1220 1941 Size: 1220 721 EcoO109I rG'GnC_Cy Cuts at: 0 1225 1941 Size: 1225 716 EcoRI G'AATT_C Cuts at: 0 1669 1941 Size: 1669 272 EcoRV GAT'ATC Cuts at: 0 1164 1266 1941 Size: 1164 102 675 Fragments arranged by size: 1164 675 102 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 470 502 1153 1185 1941 Size: 470 32 651 32 756 Fragments arranged by size: 756 651 470 32 32 HindIII A'AGCT_T Cuts at: 0 1445 1941 Size: 1445 496 KpnI G_GTAC'C Cuts at: 0 85 461 1941 Size: 85 376 1480 Fragments arranged by size: 1480 376 85 MfeI C'AATT_G Cuts at: 0 1766 1941 Size: 1766 175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 384 1015 1860 1941 Size: 384 631 845 81 Fragments arranged by size: 845 631 384 81 MslI CAynn'nnrTG Cuts at: 0 691 1941 Size: 691 1250 MspA1I CmG'CkG Cuts at: 0 207 982 1207 1941 Size: 207 775 225 734 Fragments arranged by size: 775 734 225 207 NcoI C'CATG_G Cuts at: 0 40 692 1941 Size: 40 652 1249 Fragments arranged by size: 1249 652 40 NheI G'CTAG_C Cuts at: 0 1825 1941 Size: 1825 116 NruI TCG'CGA Cuts at: 0 108 1941 Size: 108 1833 NspI r_CATG'y Cuts at: 0 657 1463 1757 1941 Size: 657 806 294 184 Fragments arranged by size: 806 657 294 184 PfoI T'CCnGG_A Cuts at: 0 1227 1941 Size: 1227 714 PpuMI rG'GwC_Cy Cuts at: 0 1225 1941 Size: 1225 716 PsiI TTA'TAA Cuts at: 0 1507 1941 Size: 1507 434 PspXI vC'TCGA_Gb Cuts at: 0 52 1941 Size: 52 1889 PstI C_TGCA'G Cuts at: 0 227 1557 1800 1941 Size: 227 1330 243 141 Fragments arranged by size: 1330 243 227 141 PvuII CAG'CTG Cuts at: 0 1207 1941 Size: 1207 734 SacII CC_GC'GG Cuts at: 0 208 1941 Size: 208 1733 SbfI CC_TGCA'GG Cuts at: 0 1557 1941 Size: 1557 384 ScaI AGT'ACT Cuts at: 0 1548 1941 Size: 1548 393 SfcI C'TryA_G Cuts at: 0 146 223 782 915 1247 1553 1657 1796 Size: 146 77 559 133 332 306 104 139 Cuts at: 1796 1941 Size: 145 Fragments arranged by size: 559 332 306 146 145 139 133 104 77 SmlI C'TyrA_G Cuts at: 0 52 1748 1941 Size: 52 1696 193 Fragments arranged by size: 1696 193 52 StuI AGG'CCT Cuts at: 0 523 1941 Size: 523 1418 StyI C'CwwG_G Cuts at: 0 40 692 1941 Size: 40 652 1249 Fragments arranged by size: 1249 652 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 715 1941 Size: 715 1226 TatI w'GTAC_w Cuts at: 0 795 1546 1941 Size: 795 751 395 Fragments arranged by size: 795 751 395 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1655 1687 1941 Size: 1655 32 254 Fragments arranged by size: 1655 254 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 690 1941 Size: 690 1251 XhoI C'TCGA_G Cuts at: 0 52 1941 Size: 52 1889 Enzymes that do cut: Acc65I AcuI AloI AlwNI ApoI AseI AvaI BanI BanII BbsI BbvCI BclI BglI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BseRI BseYI BsiEI Bsp1286I BspMI BsrBI BsrDI BssSI BstAPI BstEII BstXI Bsu36I BtgI BtsI ClaI DraI DraIII EaeI EagI Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I HindIII KpnI MfeI MmeI MslI MspA1I NcoI NheI NruI NspI PfoI PpuMI PsiI PspXI PstI PvuII SacII SbfI ScaI SfcI SmlI StuI StyI TaqII TatI TstI XcmI XhoI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglII BlpI BmgBI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BstBI BstYI BstZ17I BtgZI CspCI DrdI EarI EciI EcoICRI FalI FseI FspI FspAI HaeII HincII HpaI KasI MluI MscI NaeI NarI NdeI NgoMIV NotI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PspOMI PsrI PvuI RsrII SacI SalI SanDI SapI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XmaI XmnI ZraI