################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## Acc65I AccI AccIII AclI AflII Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AvaI AviII AvrII BamHI BanI BbeI BbvCI BcgI BciVI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaBI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspLU11I BspMI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PmeI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AatII 244 GACGTC GACGT^C AccII 485 CGCG CG^CG AcyI 244 GACGTC G[GA]^CG[CT]C AflIII 701 ACACGT A^C[GA][CT]GT AhdI 1099 GACAATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1028 CCTCAGG CC^T[ACGT]AGG AspEI 1099 GACAATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 450 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 263 TGGCCA TGG^CCA BanII 616 GAGCCC G[GA]GC[CT]^C BbrPI 348 CACGTG CAC^GTG BbsI 550 GTCTTC GAAGAC(2/6) BclI 203 TGATCA T^GATCA BcnI 1046 CCGGG CC^[GC]GG BglII 986 AGATCT A^GATCT BpuAI 550 GTCTTC GAAGAC(2/6) BsaAI 348 CACGTG [CT]AC^GT[GA] BsaHI 244 GACGTC G[GA]^CG[CT]C BsaI 1084 GAGACC GGTCTC(1/5) BsgI 919 CTGCAC GTGCAG(16/14) Bsp1407I 441 TGTACA T^GTACA BspHI 686 TCATGA T^CATGA BsrBI 3 GAGCGG CCGCTC(-3/-3) BsrGI 441 TGTACA T^GTACA BstUI 485 CGCG CG^CG BstYI 986 AGATCT [GA]^GATC[CT] Bsu36I 1028 CCTCAGG CC^T[ACGT]AGG Cac8I 726 GCAAGC GC[ACGT]^[ACGT]GC CfoI 486 GCGC GCG^C DraII 995 AGGACCT [GA]G^G[ACGT]CC[CT] EaeI 263 TGGCCA [CT]^GGCC[GA] Eam1105I 1099 GACAATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1028 CCTCAGG CC^T[ACGT]AGG EcoO109I 995 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 735 GAATTC G^AATTC FbaI 203 TGATCA T^GATCA HgaI 1105 GCGTC GACGC(5/10) HhaI 486 GCGC GCG^C Hin1I 244 GACGTC G[GA]^CG[CT]C HinP1I 486 GCGC G^CGC MflI 986 AGATCT [GA]^GATC[CT] MluNI 263 TGGCCA TGG^CCA MscI 263 TGGCCA TGG^CCA MseI 386 TTAA T^TAA MvnI 485 CGCG CG^CG MwoI 319 GCCACCGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 1046 CCGGG CC^[GC]GG PflFI 450 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 620 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 348 CACGTG CAC^GTG PmlI 348 CACGTG CAC^GTG PpuMI 995 AGGACCT [GA]G^G[AT]CC[CT] PshAI 240 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 686 TCATGA T^CATGA SgrAI 321 CACCGGTG C[GA]^CCGG[CT]G SmlI 649 CTTGAG C^T[CT][GA]AG SspBI 441 TGTACA T^GTACA SspI 190 AATATT AAT^ATT TaqI 35 TCGA T^CGA Tru9I 386 TTAA T^TAA Tth111I 450 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 35 TCGA T^CGA Van91I 620 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 986 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AgeI 322 ACCGGT A^CCGGT 425 ACCGGT AlwI 470 GATCC GGATC(4/5) 857 GATCC AspHI 105 GTGCTC G[AT]GC[AT]^C 940 GTGCTC BsaWI 322 ACCGGT [AT]^CCGG[AT] 425 ACCGGT BsiHKAI 105 GTGCTC G[AT]GC[AT]^C 940 GTGCTC BsmAI 1084 GAGAC GTCTC(1/5) 610 GTCTC BsrDI 267 CATTGC GCAATG(2/0) 341 GCAATG BsrFI 322 ACCGGT [GA]^CCGG[CT] 425 ACCGGT BsrI 41 CCAGT ACTGG(1/-1) 1079 CCAGT Cfr10I 322 ACCGGT [GA]^CCGG[CT] 425 ACCGGT EcoT14I 573 CCAAGG C^C[AT][AT]GG 719 CCTTGG FauI 5 GCGGG CCCGC(4/6) 392 GCGGG HphI 201 GGTGA GGTGA(8/7) 206 TCACC MslI 414 CATTAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 768 CATAGCTGTG NlaIV 464 GGCTCC GG[ACGT]^[ACGT]CC 615 GGAGCC PinAI 322 ACCGGT A^CCGGT 425 ACCGGT PleI 37 GACTC GAGTC(4/5) 312 GAGTC SfaNI 396 GATGC GCATC(5/9) 368 GCATC StyI 573 CCAAGG C^C[AT][AT]GG 719 CCTTGG Tsp45I 826 GTGAC ^GT[GC]AC 898 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII BbvI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI TfiI TseI Tsp509I TspRI