(Circular) (Six-base) MAPSORT of: pFN21AE1403.seq Check: 4917 from: 1 to: 5827 pFN21AE1403 5827 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2253 3637 278 Size: 53 83 186 936 717 1384 2468 Fragments arranged by size: 2468 1384 936 717 186 83 53 AccI GT'mk_AC Cuts at: 1937 3170 1937 Size: 1233 4594 Acc65I G'GTAC_C Cuts at: 3148 3148 Size: 5827 AclI AA'CG_TT Cuts at: 1563 3954 4327 1563 Size: 2391 373 3063 Fragments arranged by size: 3063 2391 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3008 3890 5274 5542 1632 Size: 1376 882 1384 268 1917 Fragments arranged by size: 1917 1384 1376 882 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5630 AflIII A'CryG_T Cuts at: 1203 2706 4726 5703 1203 Size: 1503 2020 977 1327 Fragments arranged by size: 2020 1503 1327 977 AgeI A'CCGG_T Cuts at: 2327 2430 4658 2327 Size: 103 2228 3496 Fragments arranged by size: 3496 2228 103 AhdI GACnn_n'nnGTC Cuts at: 3109 4556 3109 Size: 1447 4380 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5827 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5827 AlwNI CAG_nnn'CTG Cuts at: 3570 5142 3570 Size: 1572 4255 ApaLI G'TGCA_C Cuts at: 3884 5040 3884 Size: 1156 4671 ApoI r'AATT_y Cuts at: 1493 2023 2740 2842 3136 3362 3514 5623 1493 Size: 530 717 102 294 226 152 2109 1697 Fragments arranged by size: 2109 1697 717 530 294 226 152 102 AseI AT'TA_AT Cuts at: 160 1032 4381 160 Size: 872 3349 1606 Fragments arranged by size: 3349 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 AvaI C'yCGr_G Cuts at: 1870 1943 3152 1870 Size: 73 1209 4545 Fragments arranged by size: 4545 1209 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2588 2621 2588 Size: 33 5794 BamHI G'GATC_C Cuts at: 3157 3524 3157 Size: 367 5460 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3148 4603 618 Size: 325 217 627 1361 1455 1842 Fragments arranged by size: 1842 1455 1361 627 325 217 BanII G_rGCy'C Cuts at: 1391 2625 3146 1391 Size: 1234 521 4072 Fragments arranged by size: 4072 1234 521 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2548 928 Size: 971 649 4207 Fragments arranged by size: 4207 971 649 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4018 4052 1903 Size: 34 2081 34 3678 Fragments arranged by size: 3678 2081 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3719 4929 3719 Size: 1210 4617 BclI T'GATC_A Cuts at: 1548 2208 1548 Size: 660 5167 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4438 136 Size: 107 122 71 631 605 2766 1525 Fragments arranged by size: 2766 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2991 5822 2991 Size: 2831 2996 BlpI GC'TnA_GC Cuts at: 3244 3244 Size: 5827 Bme1580I G_kGCm'C Cuts at: 1790 3888 5044 1790 Size: 2098 1156 2573 Fragments arranged by size: 2573 2098 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5827 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3558 4516 442 Size: 416 53 83 399 24 2141 958 1753 Fragments arranged by size: 2141 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5827 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2029 2122 2524 4487 1239 Size: 790 93 402 1963 2579 Fragments arranged by size: 2579 1963 790 402 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2675 3952 4817 5115 2675 Size: 1277 865 298 3387 Fragments arranged by size: 3387 1277 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3083 4490 882 Size: 617 1584 1407 2219 Fragments arranged by size: 2219 1584 1407 617 BsaAI yAC'GTr Cuts at: 493 1204 2355 3618 493 Size: 711 1151 1263 2702 Fragments arranged by size: 2702 1263 1151 711 BsaBI GATnn'nnATC Cuts at: 5821 5821 Size: 5827 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2250 3634 4016 4651 Size: 53 83 186 936 717 1384 382 635 Cuts at: 4651 275 Size: 1451 Fragments arranged by size: 1451 1384 936 717 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2327 2430 4260 4658 4932 5079 5490 2327 Size: 103 1830 398 274 147 411 2664 Fragments arranged by size: 2664 1830 411 398 274 147 103 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5602 Fragments arranged by size: 5602 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5827 BseYI C'CCAG_C Cuts at: 5030 5526 5030 Size: 496 5331 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2909 1163 Size: 573 1173 4081 Fragments arranged by size: 4081 1173 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3277 4038 5066 1540 Size: 275 186 1276 761 1028 2301 Fragments arranged by size: 2301 1276 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2114 2949 3888 3973 5044 2114 Size: 835 939 85 1071 2897 Fragments arranged by size: 2897 1071 939 835 85 BsmI GAATG_Cn' Cuts at: 3338 3431 3338 Size: 93 5734 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2114 2625 2949 3146 3888 3973 5044 Size: 399 324 511 324 197 742 85 1071 Cuts at: 5044 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 511 399 324 324 197 85 BspEI T'CCGG_A Cuts at: 5490 5490 Size: 5827 BspHI T'CATG_A Cuts at: 2691 3716 5446 2691 Size: 1025 1730 3072 Fragments arranged by size: 3072 1730 1025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3182 844 Size: 2338 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2010 3714 1394 Size: 429 187 1704 3507 Fragments arranged by size: 3507 1704 429 187 BsrDI GCAATG_nn' Cuts at: 65 1248 2271 2353 4322 4496 65 Size: 1183 1023 82 1969 174 1396 Fragments arranged by size: 1969 1396 1183 1023 174 82 BsrFI r'CCGG_y Cuts at: 1700 1810 2327 2430 4471 4658 1700 Size: 110 517 103 2041 187 2869 Fragments arranged by size: 2869 2041 517 187 110 103 BsrGI T'GTAC_A Cuts at: 96 2446 96 Size: 2350 3477 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5827 BssSI C'ACGA_G Cuts at: 3887 4899 3887 Size: 1012 4815 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5827 BstBI TT'CG_AA Cuts at: 4631 4631 Size: 5827 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5827 BstYI r'GATC_y Cuts at: 1972 2991 3157 3524 3909 3926 5378 5464 5476 Size: 1019 166 367 385 17 1452 86 12 Cuts at: 5476 5822 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1019 385 367 346 166 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3034 3034 Size: 5827 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5275 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2046 2422 2601 487 Size: 683 56 820 376 179 3713 Fragments arranged by size: 3713 820 683 376 179 56 BtsI GCAGTG_nn' Cuts at: 2319 2926 3343 4137 4157 2319 Size: 607 417 794 20 3989 Fragments arranged by size: 3989 794 607 417 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5827 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5792 DraI TTT'AAA Cuts at: 3131 3131 Size: 5827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4834 809 Size: 507 3518 1802 Fragments arranged by size: 3518 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2268 3274 4163 Size: 54 1425 50 275 18 438 1006 889 Cuts at: 4163 5792 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1006 889 438 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3274 1537 Size: 275 1462 4090 Fragments arranged by size: 4090 1462 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3757 1353 Size: 2404 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4414 4788 4934 387 Size: 1064 138 2825 374 146 1280 Fragments arranged by size: 2825 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2029 2122 2524 3008 3890 4487 5274 Size: 393 397 93 402 484 882 597 787 Cuts at: 5274 5542 1239 Size: 268 1524 Fragments arranged by size: 1524 882 787 597 484 402 397 393 268 93 EcoO109I rG'GnC_Cy Cuts at: 3001 3001 Size: 5827 EcoRI G'AATT_C Cuts at: 2740 3136 3514 2740 Size: 396 378 5053 Fragments arranged by size: 5053 396 378 FspI TGC'GCA Cuts at: 4333 4333 Size: 5827 HaeII r_GCGC'y Cuts at: 1678 4974 1678 Size: 3296 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3095 3127 4468 4500 4542 Size: 32 135 32 1523 32 1341 32 42 Cuts at: 4542 4574 1373 Size: 32 2626 Fragments arranged by size: 2626 1523 1341 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3171 3417 669 Size: 988 281 1233 246 3079 Fragments arranged by size: 3079 1233 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5827 HpaI GTT'AAC Cuts at: 3417 3417 Size: 5827 KpnI G_GTAC'C Cuts at: 3152 3152 Size: 5827 MfeI C'AATT_G Cuts at: 3426 3426 Size: 5827 MluI A'CGCG_T Cuts at: 5703 5703 Size: 5827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4941 5125 1305 Size: 3636 184 2007 Fragments arranged by size: 3636 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2270 10 Size: 54 1425 343 438 3567 Fragments arranged by size: 3567 1425 438 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 2777 3785 4144 4303 518 Size: 648 1257 354 1008 359 159 2042 Fragments arranged by size: 2042 1257 1008 648 359 354 159 MspA1I CmG'CkG Cuts at: 1606 3243 3920 5068 5313 1606 Size: 1637 677 1148 245 2120 Fragments arranged by size: 2120 1637 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5275 NdeI CA'TA_TG Cuts at: 387 387 Size: 5827 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5827 NotI GC'GGCC_GC Cuts at: 3274 3274 Size: 5827 NspI r_CATG'y Cuts at: 1132 1229 3185 4730 1132 Size: 97 1956 1545 2229 Fragments arranged by size: 2229 1956 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5827 PciI A'CATG_T Cuts at: 4726 4726 Size: 5827 PflMI CCAn_nnn'nTGG Cuts at: 1108 2631 1108 Size: 1523 4304 PmeI GTTT'AAAC Cuts at: 3131 3131 Size: 5827 PmlI CAC'GTG Cuts at: 2355 2355 Size: 5827 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3896 3928 5409 5441 3896 Size: 32 1481 32 4282 Fragments arranged by size: 4282 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3001 3001 Size: 5827 PshAI GACnn'nnGTC Cuts at: 1613 2249 1613 Size: 636 5191 PsiI TTA'TAA Cuts at: 2226 3397 2226 Size: 1171 4656 PstI C_TGCA'G Cuts at: 830 3179 830 Size: 2349 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5827 SalI G'TCGA_C Cuts at: 1936 3169 1936 Size: 1233 4594 SbfI CC_TGCA'GG Cuts at: 3179 3179 Size: 5827 ScaI AGT'ACT Cuts at: 4075 5787 4075 Size: 1712 4115 SfcI C'TryA_G Cuts at: 826 1045 3175 4310 4991 5182 826 Size: 219 2130 1135 681 191 1471 Fragments arranged by size: 2130 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 SgrAI Cr'CCGG_yG Cuts at: 1700 2327 1700 Size: 627 5200 SmaI CCC'GGG Cuts at: 1872 3154 1872 Size: 1282 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2654 3931 4832 5094 820 Size: 197 926 711 1277 901 262 1553 Fragments arranged by size: 1553 1277 926 901 711 262 197 SnaBI TAC'GTA Cuts at: 493 3618 493 Size: 3125 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5827 SphI G_CATG'C Cuts at: 1132 3185 1132 Size: 2053 3774 SspI AAT'ATT Cuts at: 5 52 2197 3751 5 Size: 47 2145 1554 2081 Fragments arranged by size: 2145 2081 1554 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5827 StyI C'CwwG_G Cuts at: 513 1065 2578 2724 3266 513 Size: 552 1513 146 542 3074 Fragments arranged by size: 3074 1513 552 542 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2591 3854 3871 4024 4209 4654 325 Size: 1255 1011 1263 17 153 185 445 1498 Fragments arranged by size: 1498 1263 1255 1011 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2446 3024 4073 Size: 274 80 33 51 1443 469 578 1049 Cuts at: 4073 5785 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1049 578 469 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2993 3025 5787 5819 2993 Size: 32 2762 32 3001 Fragments arranged by size: 3001 2762 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2458 1316 Size: 1142 4685 XbaI T'CTAG_A Cuts at: 3163 3163 Size: 5827 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5827 XmaI C'CCGG_G Cuts at: 1870 3152 1870 Size: 1282 4545 XmnI GAAnn'nnTTC Cuts at: 3956 3956 Size: 5827 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2251 3635 276 Size: 53 83 186 936 717 1384 2468 Fragments arranged by size: 2468 1384 936 717 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI