################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BbeI BbrPI BcgI BcnI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 508 GTAGAC GT^[AC][GT]AC AccII 1128 CGCG CG^CG ApaI 400 GGGCCC GGGCC^C AspHI 1222 GAGCAC G[AT]GC[AT]^C BalI 485 TGGCCA TGG^CCA BanI 360 GGCACC G^G[CT][GA]CC BbsI 741 GAAGAC GAAGAC(2/6) BciVI 328 GTATCC GTATCC(6/5) BclI 870 TGATCA T^GATCA BglI 685 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 741 GAAGAC GAAGAC(2/6) BsiHKAI 1222 GAGCAC G[AT]GC[AT]^C BsmBI 1107 CGTCTC CGTCTC(1/5) BsmFI 49 GGGAC GGGAC(10/14) Bsp1407I 548 TGTACA T^GTACA BspHI 873 TCATGA T^CATGA BsrGI 548 TGTACA T^GTACA BssSI 1219 CACGAG CACGAG(-5/-1) BstF5I 168 CATCC GGATG(2/0) BstUI 1128 CGCG CG^CG BstXI 179 CCATGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 1053 GCGC GCG^C DraII 1097 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO109I 1097 GGGGCCT [GA]G^G[ACGT]CC[CT] FbaI 870 TGATCA T^GATCA FokI 168 CATCC GGATG(9/13) HapII 191 CCGG C^CGG HhaI 1053 GCGC GCG^C HinP1I 1053 GCGC G^CGC HinfI 183 GAATC G^A[ACGT]TC HpaII 191 CCGG C^CGG MluNI 485 TGGCCA TGG^CCA MscI 485 TGGCCA TGG^CCA MslI 282 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 191 CCGG C^CGG MvnI 1128 CGCG CG^CG RcaI 873 TCATGA T^CATGA SapI 981 GCTCTTC GCTCTTC(1/4) SpeI 52 ACTAGT A^CTAGT SspBI 548 TGTACA T^GTACA TfiI 183 GAATC G^A[AT]TC XcmI 473 CCAAGACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AciI 465 CCGC CCGC(-3/-1) 1129 GCGG AcsI 781 AAATTC [GA]^AATT[CT] 1072 AAATTT AlwI 558 GATCC GGATC(4/5) 587 GATCC ApoI 781 AAATTC [GA]^AATT[CT] 1072 AAATTT BanII 97 GAGCCC G[GA]GC[CT]^C 400 GGGCCC BbvCI 86 CCTCAGC CCTCAGC(-5/-2) 963 GCTGAGG BbvI 270 GCAGC GCAGC(8/12) 978 GCTGC BsaI 311 GAGACC GGTCTC(1/5) 991 GAGACC BseRI 300 CTCCTC GAGGAG(10/8) 492 CTCCTC BsrDI 801 CATTGC GCAATG(2/0) 520 GCAATG BstYI 557 AGATCC [GA]^GATC[CT] 586 AGATCC Cac8I 483 GCTGGC GC[ACGT]^[ACGT]GC 690 GCAGGC EaeI 188 TGGCCG [CT]^GGCC[GA] 485 TGGCCA EarI 982 CTCTTC CTCTTC(1/4) 1110 CTCTTC EcoT14I 101 CCTTGG C^C[AT][AT]GG 530 CCTTGG Fnu4HI 270 GCAGC GC^[ACGT]GC 978 GCTGC ItaI 270 GCAGC GC^[ACGT]GC 978 GCTGC Ksp632I 982 CTCTTC CTCTTC(1/4) 1110 CTCTTC MflI 557 AGATCC [GA]^GATC[CT] 586 AGATCC MseI 770 TTAA T^TAA 1139 TTAA MspA1I 44 CAGCTG C[AC]G^C[GT]G 961 CAGCTG PvuII 44 CAGCTG CAG^CTG 961 CAGCTG ScaI 120 AGTACT AGT^ACT 673 AGTACT StyI 101 CCTTGG C^C[AT][AT]GG 530 CCTTGG Tru9I 770 TTAA T^TAA 1139 TTAA TseI 270 GCAGC G^C[AT]GC 978 GCTGC Tsp45I 139 GTGAC ^GT[GC]AC 856 GTCAC XhoII 557 AGATCC [GA]^GATC[CT] 586 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AfaI AluI BfaI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Cfr13I DdeI DpnII EcoRII HaeIII HphI MaeI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI Tsp509I TspRI