(Circular) (Six-base) MAPSORT of: pFN21AE6310.seq Check: 5605 from: 1 to: 5956 pFN21AE6310 5956 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2514 3299 1937 Size: 577 785 4594 Fragments arranged by size: 4594 785 577 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2406 4019 5403 5671 1632 Size: 774 1613 1384 268 1917 Fragments arranged by size: 1917 1613 1384 774 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5956 AlwNI CAG_nnn'CTG Cuts at: 3699 5271 3699 Size: 1572 4384 ApaI G_GGCC'C Cuts at: 2409 2409 Size: 5956 ApaLI G'TGCA_C Cuts at: 4013 5169 4013 Size: 1156 4800 ApoI r'AATT_y Cuts at: 1493 2786 3077 3265 3491 3643 5752 1493 Size: 1293 291 188 226 152 2109 1697 Fragments arranged by size: 2109 1697 1293 291 226 188 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 3281 1870 Size: 73 1338 4545 Fragments arranged by size: 4545 1338 73 BamHI G'GATC_C Cuts at: 3286 3653 3286 Size: 367 5589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2365 3277 4732 618 Size: 325 217 627 578 912 1455 1842 Fragments arranged by size: 1842 1455 912 627 578 325 217 BanII G_rGCy'C Cuts at: 1391 2106 2409 3275 1391 Size: 715 303 866 4072 Fragments arranged by size: 4072 866 715 303 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2753 928 Size: 971 854 4131 Fragments arranged by size: 4131 971 854 BbvCI CC'TCA_GC Cuts at: 2092 2969 2092 Size: 877 5079 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4147 4181 1903 Size: 34 2210 34 3678 Fragments arranged by size: 3678 2210 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2344 3848 5058 2344 Size: 1504 1210 3242 Fragments arranged by size: 3242 1504 1210 BclI T'GATC_A Cuts at: 1548 2875 1548 Size: 1327 4629 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2696 4567 136 Size: 107 122 71 631 605 1024 1871 1525 Fragments arranged by size: 1871 1525 1024 631 605 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 2368 2409 2856 4017 5173 1790 Size: 578 41 447 1161 1156 2573 Fragments arranged by size: 2573 1161 1156 578 447 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2371 2403 3687 Size: 416 53 83 399 24 954 32 1284 Cuts at: 3687 4645 442 Size: 958 1753 Fragments arranged by size: 1753 1284 958 954 416 399 83 53 32 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4616 1239 Size: 3377 2579 Bpu10I CC'TnA_GC Cuts at: 2030 2092 2715 2969 2030 Size: 62 623 254 5017 Fragments arranged by size: 5017 623 254 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4081 4946 5244 4081 Size: 865 298 4793 Fragments arranged by size: 4793 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2310 2990 4619 882 Size: 617 811 680 1629 2219 Fragments arranged by size: 2219 1629 811 680 617 BsaAI yAC'GTr Cuts at: 493 1204 3747 493 Size: 711 2543 2702 Fragments arranged by size: 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 5950 5950 Size: 5956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3763 4145 4780 275 Size: 53 83 186 936 2230 382 635 1451 Fragments arranged by size: 2230 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5731 Fragments arranged by size: 5731 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2296 2488 1186 Size: 1110 192 4654 Fragments arranged by size: 4654 1110 192 BseYI C'CCAG_C Cuts at: 2051 2168 2900 5159 5655 2051 Size: 117 732 2259 496 2352 Fragments arranged by size: 2352 2259 732 496 117 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5383 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3406 4167 5195 1540 Size: 275 186 1405 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3231 4017 4102 5173 3231 Size: 786 85 1071 4014 Fragments arranged by size: 4014 1071 786 85 BsmI GAATG_Cn' Cuts at: 3467 3560 3467 Size: 93 5863 BsmBI CGTCTCn'nnnn_ Cuts at: 3118 3118 Size: 5956 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2368 2409 2856 3231 3275 4017 Size: 399 316 262 41 447 375 44 742 Cuts at: 4017 4102 5173 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 447 399 375 316 262 85 44 41 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 2878 3845 5575 2878 Size: 967 1730 3259 Fragments arranged by size: 3259 1730 967 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3311 844 Size: 2467 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3843 1394 Size: 429 2020 3507 Fragments arranged by size: 3507 2020 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2532 2805 4451 4625 65 Size: 1183 1284 273 1646 174 1396 Fragments arranged by size: 1646 1396 1284 1183 273 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4600 4787 1700 Size: 110 2790 187 2869 Fragments arranged by size: 2869 2790 187 110 BsrGI T'GTAC_A Cuts at: 96 2553 96 Size: 2457 3499 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 3224 4016 5028 3224 Size: 792 1012 4152 Fragments arranged by size: 4152 1012 792 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5956 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstXI CCAn_nnnn'nTGG Cuts at: 2191 2191 Size: 5956 BstYI r'GATC_y Cuts at: 1972 2562 2591 3286 3653 4038 4055 5507 5593 Size: 590 29 695 367 385 17 1452 86 Cuts at: 5593 5605 5951 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 695 590 385 367 346 86 29 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4465 Fragments arranged by size: 4465 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5217 Fragments arranged by size: 5217 683 56 BtsI GCAGTG_nn' Cuts at: 3472 4266 4286 3472 Size: 794 20 5142 Fragments arranged by size: 5142 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2490 3403 Size: 54 1425 50 275 18 363 297 913 Cuts at: 3403 4292 5921 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 913 889 363 297 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2993 3121 3886 1353 Size: 1640 128 765 3423 Fragments arranged by size: 3423 1640 765 128 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4543 4917 5063 387 Size: 1064 138 2954 374 146 1280 Fragments arranged by size: 2954 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2406 4019 4616 5403 5671 1239 Size: 393 774 1613 597 787 268 1524 Fragments arranged by size: 1613 1524 787 774 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3103 3103 Size: 5956 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 5103 1678 Size: 3425 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2318 2350 4597 4629 4671 Size: 32 135 32 746 32 2247 32 42 Cuts at: 4671 4703 1373 Size: 32 2626 Fragments arranged by size: 2626 2247 746 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3300 3546 669 Size: 988 281 1362 246 3079 Fragments arranged by size: 3079 1362 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5070 5254 1305 Size: 3765 184 2007 Fragments arranged by size: 3765 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2492 10 Size: 54 1425 343 660 3474 Fragments arranged by size: 3474 1425 660 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2291 3914 4273 4432 518 Size: 648 1125 1623 359 159 2042 Fragments arranged by size: 2042 1623 1125 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2051 2968 3372 4049 5197 5442 1606 Size: 445 917 404 677 1148 245 2120 Fragments arranged by size: 2120 1148 917 677 445 404 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4465 Fragments arranged by size: 4465 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 3314 4859 1132 Size: 97 2085 1545 2229 Fragments arranged by size: 2229 2085 1545 97 PasI CC'CwG_GG Cuts at: 1348 2321 1348 Size: 973 4983 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 5054 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PspOMI G'GGCC_C Cuts at: 2405 2405 Size: 5956 PstI C_TGCA'G Cuts at: 830 3308 830 Size: 2478 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 PvuII CAG'CTG Cuts at: 2051 2968 2051 Size: 917 5039 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SapI GCTCTTCn'nnn_ Cuts at: 2993 2993 Size: 5956 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 2127 2680 4204 5916 2127 Size: 553 1524 1712 2167 Fragments arranged by size: 2167 1712 1524 553 SfcI C'TryA_G Cuts at: 826 1045 2046 2568 2674 3304 4439 5120 5311 Size: 219 1001 522 106 630 1135 681 191 Cuts at: 5311 826 Size: 1471 Fragments arranged by size: 1471 1135 1001 681 630 522 219 191 106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3283 1872 Size: 1411 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4060 4961 5223 820 Size: 197 926 2117 901 262 1553 Fragments arranged by size: 2117 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 2057 152 Size: 1905 4051 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5956 StyI C'CwwG_G Cuts at: 513 1065 2004 2106 2535 3395 513 Size: 552 939 102 429 860 3074 Fragments arranged by size: 3074 939 860 552 429 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3983 4000 4153 4338 4783 325 Size: 1255 2403 17 153 185 445 1498 Fragments arranged by size: 2403 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2125 2553 2678 Size: 274 80 33 51 1443 148 428 125 Cuts at: 2678 4202 5914 96 Size: 1524 1712 138 Fragments arranged by size: 1712 1524 1443 428 274 148 138 125 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2854 2886 5916 5948 2854 Size: 32 3030 32 2862 Fragments arranged by size: 3030 2862 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2485 2485 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 3281 1870 Size: 1411 4545 XmnI GAAnn'nnTTC Cuts at: 4085 4085 Size: 5956 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI