################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccII AccIII AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BcnI BfrI BlnI BpuAI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstPI BstUI BstXI Bsu36I CfoI Cfr10I ClaI CpoI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV FauI FseI FspI HaeII HapII HhaI HinP1I HpaII KasI KpnI KspI MluI MroI MspA1I MspI MvnI NaeI NarI NciI NgoMIV NlaIV NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 1639 GTATAC GT^[AC][GT]AC AciI 1881 GCGG CCGC(-3/-1) AclI 1951 AACGTT AA^CGTT AcyI 2441 GACGCC G[GA]^CG[CT]C AflIII 2087 ACACGT A^C[GA][CT]GT AlwNI 2105 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 2476 ATTAAT AT^TAAT AsnI 2476 ATTAAT AT^TAAT BalI 1565 TGGCCA TGG^CCA BglI 2166 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 657 AGATCT A^GATCT BlpI 268 GCTAAGC GC^T[ACGT]AGC Bpu1102I 268 GCTAAGC GC^T[ACGT]AGC BsaAI 2088 CACGTA [CT]AC^GT[GA] BsaBI 1069 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2441 GACGCC G[GA]^CG[CT]C BseRI 2423 CTCCTC GAGGAG(10/8) BsmFI 2503 GGGAC GGGAC(10/14) BsmI 952 GCATTC GAATGC(1/-1) BspHI 147 TCATGA T^CATGA BssSI 1511 CTCGTG CACGAG(-5/-1) Bst1107I 1639 GTATAC GTA^TAC BstAPI 376 GCAATGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 657 AGATCT [GA]^GATC[CT] BstZ17I 1639 GTATAC GTA^TAC CelII 268 GCTAAGC GC^T[ACGT]AGC Cfr13I 2080 GGCCC G^G[ACGT]CC DraIII 1424 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 2209 CCATGG C^C[GA][CT]GG EaeI 1565 TGGCCA [CT]^GGCC[GA] HgaI 2441 GACGC GACGC(5/10) Hin1I 2441 GACGCC G[GA]^CG[CT]C HincII 1954 GTTAAC GT[CT]^[GA]AC HindII 1954 GTTAAC GT[CT]^[GA]AC HpaI 1954 GTTAAC GTT^AAC MamI 1069 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 461 CAATTG C^AATTG MflI 657 AGATCT [GA]^GATC[CT] MluNI 1565 TGGCCA TGG^CCA MscI 1565 TGGCCA TGG^CCA MunI 461 CAATTG C^AATTG NcoI 2209 CCATGG C^CATGG NdeI 2233 CATATG CA^TATG NheI 747 GCTAGC G^CTAGC PflMI 1568 CCAAGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 2476 ATTAAT AT^TAAT Psp1406I 1951 AACGTT AA^CGTT RcaI 147 TCATGA T^CATGA SapI 154 GCTCTTC GCTCTTC(1/4) Sau96I 2080 GGCCC G^G[ACGT]CC SmlI 541 CTTGAG C^T[CT][GA]AG Van91I 1568 CCAAGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 657 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwI 688 GATCC GGATC(4/5) 1420 GGATC AspHI 769 GAGCAC G[AT]GC[AT]^C 1924 GAGCAC BbvCI 217 CCTCAGC CCTCAGC(-5/-2) 2569 GCTGAGG BclI 566 TGATCA T^GATCA 897 TGATCA BpmI 365 CTGGAG CTGGAG(16/14) 2032 CTGGAG BsiHKAI 769 GAGCAC G[AT]GC[AT]^C 1924 GAGCAC BsrDI 199 GCAATG GCAATG(2/0) 376 GCAATG BstF5I 563 GGATG GGATG(2/0) 1377 GGATG EarI 155 CTCTTC CTCTTC(1/4) 1276 GAAGAG EcoNI 691 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 916 CCTACTGAAGG EcoT14I 2209 CCATGG C^C[AT][AT]GG 2525 CCTTGG EcoT22I 950 ATGCAT ATGCA^T 1247 ATGCAT FbaI 566 TGATCA T^GATCA 897 TGATCA FokI 563 GGATG GGATG(9/13) 1377 GGATG Ksp632I 155 CTCTTC CTCTTC(1/4) 1276 GAAGAG NsiI 950 ATGCAT ATGCA^T 1247 ATGCAT StyI 2209 CCATGG C^C[AT][AT]GG 2525 CCTTGG TaqI 876 TCGA T^CGA 2439 TCGA Tsp45I 178 GTCAC ^GT[GC]AC 649 GTCAC TthHB8I 876 TCGA T^CGA 2439 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BfaI BmyI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Cac8I DdeI DpnII DraI EcoRII Fnu4HI HaeIII HindIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII PleI RsaI Sau3AI ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp509I TspRI