(Circular) (Six-base) MAPSORT of: pFN21AE1094.seq Check: 8835 from: 1 to: 7438 pFN21AE1094 7438 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5248 278 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3645 4781 1937 Size: 1708 1136 4594 Fragments arranged by size: 4594 1708 1136 Acc65I G'GTAC_C Cuts at: 4759 4759 Size: 7438 AclI AA'CG_TT Cuts at: 1563 3957 5565 5938 1563 Size: 2394 1608 373 3063 Fragments arranged by size: 3063 2394 1608 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2104 2946 4346 4392 5501 6885 7153 1632 Size: 472 842 1400 46 1109 1384 268 1917 Fragments arranged by size: 1917 1400 1384 1109 842 472 268 46 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7241 AflIII A'CryG_T Cuts at: 1203 4092 6337 7314 1203 Size: 2889 2245 977 1327 Fragments arranged by size: 2889 2245 1327 977 AgeI A'CCGG_T Cuts at: 6269 6269 Size: 7438 AhdI GACnn_n'nnGTC Cuts at: 6167 6167 Size: 7438 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7438 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7438 AlwNI CAG_nnn'CTG Cuts at: 4115 5181 6753 4115 Size: 1066 1572 4800 Fragments arranged by size: 4800 1572 1066 ApaLI G'TGCA_C Cuts at: 5495 6651 5495 Size: 1156 6282 ApoI r'AATT_y Cuts at: 1493 2126 2147 3966 4055 4351 4747 4973 5125 Size: 633 21 1819 89 296 396 226 152 Cuts at: 5125 7234 1493 Size: 2109 1697 Fragments arranged by size: 2109 1819 1697 633 396 296 226 152 89 21 AseI AT'TA_AT Cuts at: 160 1032 4482 5992 160 Size: 872 3450 1510 1606 Fragments arranged by size: 3450 1606 1510 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 AvaI C'yCGr_G Cuts at: 1870 1943 4763 1870 Size: 73 2820 4545 Fragments arranged by size: 4545 2820 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4477 4510 4477 Size: 33 7405 BamHI G'GATC_C Cuts at: 4768 5135 4768 Size: 367 7071 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4759 6214 618 Size: 325 217 627 2972 1455 1842 Fragments arranged by size: 2972 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4757 1391 Size: 3366 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6467 BbvCI CC'TCA_GC Cuts at: 2223 4575 2223 Size: 2352 5086 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5629 5663 1903 Size: 34 3692 34 3678 Fragments arranged by size: 3692 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5330 6540 5330 Size: 1210 6228 BclI T'GATC_A Cuts at: 1548 2571 2902 1548 Size: 1023 331 6084 Fragments arranged by size: 6084 1023 331 BfrBI ATG'CAT Cuts at: 2957 3254 2957 Size: 297 7141 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4177 6049 136 Size: 107 122 71 631 605 2505 1872 1525 Fragments arranged by size: 2505 1872 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2662 7433 2662 Size: 4771 2667 BlpI GC'TnA_GC Cuts at: 2274 4855 2274 Size: 2581 4857 Bme1580I G_kGCm'C Cuts at: 1790 4247 5499 6655 1790 Size: 2457 1252 1156 2573 Fragments arranged by size: 2573 2457 1252 1156 BmgBI CAC'GTC Cuts at: 1318 2099 1318 Size: 781 6657 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3696 5169 6127 Size: 416 53 83 399 24 2279 1473 958 Cuts at: 6127 442 Size: 1753 Fragments arranged by size: 2279 1753 1473 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2756 1056 Size: 1700 5738 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2391 4058 6098 1239 Size: 1152 1667 2040 2579 Fragments arranged by size: 2579 2040 1667 1152 Bpu10I CC'TnA_GC Cuts at: 2223 4575 2223 Size: 2352 5086 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2567 5563 6428 6726 2567 Size: 2996 865 298 3279 Fragments arranged by size: 3279 2996 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6101 882 Size: 617 4602 2219 Fragments arranged by size: 4602 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4095 5229 493 Size: 711 2891 1134 2702 Fragments arranged by size: 2891 2702 1134 711 BsaBI GATnn'nnATC Cuts at: 3078 7432 3078 Size: 4354 3084 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4447 5245 5627 6262 Size: 53 83 186 936 2914 798 382 635 Cuts at: 6262 275 Size: 1451 Fragments arranged by size: 2914 1451 936 798 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5871 6269 6543 6690 7101 5871 Size: 398 274 147 411 6208 Fragments arranged by size: 6208 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4526 4542 4556 4572 1178 Size: 30 165 30 3123 16 14 16 4044 Fragments arranged by size: 4044 3123 165 30 30 16 16 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4419 1186 Size: 3233 4205 BseYI C'CCAG_C Cuts at: 6641 7137 6641 Size: 496 6942 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2121 3233 3480 3517 3709 1163 Size: 573 385 1112 247 37 192 4892 Fragments arranged by size: 4892 1112 573 385 247 192 37 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4888 5649 6677 1540 Size: 275 186 2887 761 1028 2301 Fragments arranged by size: 2887 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2778 3933 5499 5584 6655 2778 Size: 1155 1566 85 1071 3561 Fragments arranged by size: 3561 1566 1155 1071 85 BsmI GAATG_Cn' Cuts at: 2957 4949 5042 2957 Size: 1992 93 5353 Fragments arranged by size: 5353 1992 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2778 3933 4247 4757 5499 5584 6655 Size: 399 988 1155 314 510 742 85 1071 Cuts at: 6655 1391 Size: 2174 Fragments arranged by size: 2174 1155 1071 988 742 510 399 314 85 BspEI T'CCGG_A Cuts at: 7101 7101 Size: 7438 BspHI T'CATG_A Cuts at: 2152 5327 7057 2152 Size: 3175 1730 2533 Fragments arranged by size: 3175 2533 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4793 844 Size: 3949 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5325 1394 Size: 429 3502 3507 Fragments arranged by size: 3507 3502 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2211 2388 5933 6107 65 Size: 1183 963 177 3545 174 1396 Fragments arranged by size: 3545 1396 1183 963 177 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6082 6269 1700 Size: 110 4272 187 2869 Fragments arranged by size: 4272 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7438 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7438 BssSI C'ACGA_G Cuts at: 3516 5498 6510 3516 Size: 1982 1012 4444 Fragments arranged by size: 4444 1982 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2387 794 Size: 1593 5845 BstBI TT'CG_AA Cuts at: 6242 6242 Size: 7438 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7438 BstYI r'GATC_y Cuts at: 1972 2662 4768 5135 5520 5537 6989 7075 7087 Size: 690 2106 367 385 17 1452 86 12 Cuts at: 7087 7433 1972 Size: 346 1977 Fragments arranged by size: 2106 1977 1452 690 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3646 3646 Size: 7438 BtgI C'CryG_G Cuts at: 513 1065 2004 4214 513 Size: 552 939 2210 3737 Fragments arranged by size: 3737 2210 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6699 Fragments arranged by size: 6699 683 56 BtsI GCAGTG_nn' Cuts at: 4954 5748 5768 4954 Size: 794 20 6624 Fragments arranged by size: 6624 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7438 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7403 DraI TTT'AAA Cuts at: 2334 2586 2743 4630 4663 4742 2334 Size: 252 157 1887 33 79 5030 Fragments arranged by size: 5030 1887 252 157 79 33 DraIII CAC_nnn'GTG Cuts at: 3434 3434 Size: 7438 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6445 809 Size: 507 5129 1802 Fragments arranged by size: 5129 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3570 4885 5774 Size: 54 1425 50 275 18 1740 1315 889 Cuts at: 5774 7403 8 Size: 1629 43 Fragments arranged by size: 1740 1629 1425 1315 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4885 1537 Size: 275 3073 4090 Fragments arranged by size: 4090 3073 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2166 3276 5368 1353 Size: 813 1110 2092 3423 Fragments arranged by size: 3423 2092 1110 813 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6025 6399 6545 387 Size: 1064 138 4436 374 146 1280 Fragments arranged by size: 4436 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2104 2391 2946 4058 4346 4392 5501 Size: 393 472 287 555 1112 288 46 1109 Cuts at: 5501 6098 6885 7153 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1112 1109 787 597 555 472 393 288 287 268 46 EcoNI CCTnn'n_nnAGG Cuts at: 2700 2925 2700 Size: 225 7213 EcoRI G'AATT_C Cuts at: 4747 5125 4747 Size: 378 7060 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2951 2951 Size: 7438 FspI TGC'GCA Cuts at: 5944 5944 Size: 7438 HaeII r_GCGC'y Cuts at: 1678 6585 1678 Size: 4907 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3479 3511 4568 4600 6079 Size: 32 135 32 1907 32 1057 32 1479 Cuts at: 6079 6111 6153 6185 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1907 1479 1057 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3961 4782 5028 669 Size: 988 281 2023 821 246 3079 Fragments arranged by size: 3079 2023 988 821 281 246 HindIII A'AGCT_T Cuts at: 748 2276 2588 4204 4632 748 Size: 1528 312 1616 428 3554 Fragments arranged by size: 3554 1616 1528 428 312 HpaI GTT'AAC Cuts at: 3961 5028 3961 Size: 1067 6371 KpnI G_GTAC'C Cuts at: 4763 4763 Size: 7438 MfeI C'AATT_G Cuts at: 2466 5037 2466 Size: 2571 4867 MluI A'CGCG_T Cuts at: 7314 7314 Size: 7438 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3385 6552 6736 1305 Size: 2080 3167 184 2007 Fragments arranged by size: 3167 2080 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3572 10 Size: 54 1425 343 1740 3876 Fragments arranged by size: 3876 1740 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2178 2364 2631 2853 4127 4614 5396 Size: 648 1012 186 267 222 1274 487 782 Cuts at: 5396 5755 5914 518 Size: 359 159 2042 Fragments arranged by size: 2042 1274 1012 782 648 487 359 267 222 186 159 MspA1I CmG'CkG Cuts at: 1606 4854 5531 6679 6924 1606 Size: 3248 677 1148 245 2120 Fragments arranged by size: 3248 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 4214 513 Size: 552 939 2210 3737 Fragments arranged by size: 3737 2210 939 552 NdeI CA'TA_TG Cuts at: 387 4239 387 Size: 3852 3586 NheI G'CTAG_C Cuts at: 1052 2752 1052 Size: 1700 5738 NotI GC'GGCC_GC Cuts at: 4885 4885 Size: 7438 NsiI A_TGCA'T Cuts at: 2959 3256 2959 Size: 297 7141 NspI r_CATG'y Cuts at: 1132 1229 4796 6341 1132 Size: 97 3567 1545 2229 Fragments arranged by size: 3567 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7438 PciI A'CATG_T Cuts at: 6337 6337 Size: 7438 PflMI CCAn_nnn'nTGG Cuts at: 1108 3579 1108 Size: 2471 4967 PmeI GTTT'AAAC Cuts at: 4742 4742 Size: 7438 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5507 5539 7020 7052 5507 Size: 32 1481 32 5893 Fragments arranged by size: 5893 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7438 PsiI TTA'TAA Cuts at: 2528 5008 2528 Size: 2480 4958 PstI C_TGCA'G Cuts at: 830 4790 830 Size: 3960 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7438 SalI G'TCGA_C Cuts at: 1936 4780 1936 Size: 2844 4594 SapI GCTCTTCn'nnn_ Cuts at: 2166 2166 Size: 7438 SbfI CC_TGCA'GG Cuts at: 4790 4790 Size: 7438 ScaI AGT'ACT Cuts at: 5686 7398 5686 Size: 1712 5726 SfcI C'TryA_G Cuts at: 826 1045 2338 2658 2677 2700 4786 5921 6602 Size: 219 1293 320 19 23 2086 1135 681 Cuts at: 6602 6793 826 Size: 191 1471 Fragments arranged by size: 2086 1471 1293 1135 681 320 219 191 23 19 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7438 SmaI CCC'GGG Cuts at: 1872 4765 1872 Size: 2893 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2546 5542 6443 6705 820 Size: 197 926 603 2996 901 262 1553 Fragments arranged by size: 2996 1553 926 901 603 262 197 SnaBI TAC'GTA Cuts at: 493 5229 493 Size: 4736 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7438 SphI G_CATG'C Cuts at: 1132 4796 1132 Size: 3664 3774 SspI AAT'ATT Cuts at: 5 52 5362 5 Size: 47 5310 2081 Fragments arranged by size: 5310 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7438 StyI C'CwwG_G Cuts at: 513 1065 2004 4214 4530 4877 513 Size: 552 939 2210 316 347 3074 Fragments arranged by size: 3074 2210 939 552 347 316 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2362 5465 5482 5635 5820 6265 325 Size: 1255 782 3103 17 153 185 445 1498 Fragments arranged by size: 3103 1498 1255 782 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4072 5684 7396 Size: 274 80 33 51 1443 2095 1612 1712 Cuts at: 7396 96 Size: 138 Fragments arranged by size: 2095 1712 1612 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7398 7430 7398 Size: 32 7406 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7438 XbaI T'CTAG_A Cuts at: 4774 4774 Size: 7438 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7438 XmaI C'CCGG_G Cuts at: 1870 4763 1870 Size: 2893 4545 XmnI GAAnn'nnTTC Cuts at: 5567 5567 Size: 7438 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5246 276 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BsmBI BstXI Bsu36I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI