################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbrPI BbvCI BcgI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BseRI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI BstXI Bsu36I CelII Cfr10I ClaI CpoI DraII DrdI Eam1105I Eco47III Eco81I EcoO109I EcoO65I EcoRI EcoRV FseI FspI HgaI KpnI MamI MluI MroI NaeI NdeI NgoMIV NheI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SpeI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AflII 1189 CTTAAG C^TTAAG AflIII 1812 ACGTGT A^C[GA][CT]GT AspHI 82 GTGCTC G[AT]GC[AT]^C AvaII 1622 GGACC G^G[AT]CC BalI 1035 TGGCCA TGG^CCA BciVI 334 GGATAC GTATCC(6/5) BclI 1476 TGATCA T^GATCA BfrI 1189 CTTAAG C^TTAAG BpmI 604 CTCCAG CTGGAG(16/14) BsgI 803 GTGCAG GTGCAG(16/14) BsiEI 8 CGGCCG CG[GA][CT]^CG BsiHKAI 82 GTGCTC G[AT]GC[AT]^C BsiWI 77 CGTACG C^GTACG BsrDI 1683 CATTGC GCAATG(2/0) BstAPI 730 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 952 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 8 CGGCCG C^GGCCG EclXI 8 CGGCCG C^GGCCG Eco52I 8 CGGCCG C^GGCCG EcoNI 1663 CCTGACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1476 TGATCA T^GATCA HindIII 757 AAGCTT A^AGCTT HpaI 1851 GTTAAC GTT^AAC MfeI 1946 CAATTG C^AATTG MluNI 1035 TGGCCA TGG^CCA MscI 1035 TGGCCA TGG^CCA MunI 1946 CAATTG C^AATTG NotI 7 GCGGCCGC GC^GGCCGC SfcI 1219 CTACAG C^T[GA][CT]AG SmlI 1189 CTTAAG C^T[CT][GA]AG SnaBI 1060 TACGTA TAC^GTA SphI 1880 GCATGC GCATG^C XcmI 1927 CCAAAGAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 1230 GTATAC GT^[AC][GT]AC 1839 GTATAC AcyI 63 GGCGCC G[GA]^CG[CT]C 493 GGCGCC AfaI 78 GTAC GT^AC 1415 GTAC AlwI 1195 GGATC GGATC(4/5) 1759 GGATC BbeI 63 GGCGCC GGCGC^C 493 GGCGCC BcnI 67 CCCGG CC^[GC]GG 108 CCGGG BsaHI 63 GGCGCC G[GA]^CG[CT]C 493 GGCGCC Bst1107I 1230 GTATAC GTA^TAC 1839 GTATAC BstYI 1195 GGATCT [GA]^GATC[CT] 1759 GGATCT BstZ17I 1230 GTATAC GTA^TAC 1839 GTATAC EcoT14I 400 CCATGG C^C[AT][AT]GG 940 CCATGG EcoT22I 301 ATGCAT ATGCA^T 1583 ATGCAT HaeII 63 GGCGCC [GA]GCGC^[CT] 493 GGCGCC HapII 68 CCGG C^CGG 108 CCGG Hin1I 63 GGCGCC G[GA]^CG[CT]C 493 GGCGCC HincII 744 GTTGAC GT[CT]^[GA]AC 1851 GTTAAC HindII 744 GTTGAC GT[CT]^[GA]AC 1851 GTTAAC HpaII 68 CCGG C^CGG 108 CCGG KasI 63 GGCGCC G^GCGCC 493 GGCGCC KspI 11 CCGCGG CCGC^GG 32 CCGCGG MflI 1195 GGATCT [GA]^GATC[CT] 1759 GGATCT MslI 1327 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1911 CATCCCTGTG MspI 68 CCGG C^CGG 108 CCGG NarI 63 GGCGCC GG^CGCC 493 GGCGCC NciI 67 CCCGG CC^[GC]GG 108 CCGGG NcoI 400 CCATGG C^CATGG 940 CCATGG NsiI 301 ATGCAT ATGCA^T 1583 ATGCAT NspI 299 ACATGC [GA]CATG^[CT] 1880 GCATGC PvuII 457 CAGCTG CAG^CTG 1273 CAGCTG RsaI 78 GTAC GT^AC 1415 GTAC SacII 11 CCGCGG CCGC^GG 32 CCGCGG SfoI 63 GGCGCC GGC^GCC 493 GGCGCC StyI 400 CCATGG C^C[AT][AT]GG 940 CCATGG XhoII 1195 GGATCT [GA]^GATC[CT] 1759 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AcsI AluI ApoI Asp700I BanI BanII BbsI BbvI BfaI BmyI BpuAI BsaAI BsaI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV PflMI PleI Sau3AI Sau96I ScrFI SfaNI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XmnI