(Circular) (Six-base) MAPSORT of: pFN21AE4554.seq Check: 1955 from: 1 to: 6712 pFN21AE4554 6712 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4522 278 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3236 3845 4055 1937 Size: 1299 609 210 4594 Fragments arranged by size: 4594 1299 609 210 Acc65I G'GTAC_C Cuts at: 4033 4033 Size: 6712 AclI AA'CG_TT Cuts at: 1563 4839 5212 1563 Size: 3276 373 3063 Fragments arranged by size: 3276 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2444 2796 3050 4775 6159 6427 1632 Size: 812 352 254 1725 1384 268 1917 Fragments arranged by size: 1917 1725 1384 812 352 268 254 AflII C'TTAA_G Cuts at: 820 1017 3194 820 Size: 197 2177 4338 Fragments arranged by size: 4338 2177 197 AflIII A'CryG_T Cuts at: 1203 3817 4009 5611 6588 1203 Size: 2614 192 1602 977 1327 Fragments arranged by size: 2614 1602 1327 977 192 AgeI A'CCGG_T Cuts at: 5543 5543 Size: 6712 AhdI GACnn_n'nnGTC Cuts at: 5441 5441 Size: 6712 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6712 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6712 AlwNI CAG_nnn'CTG Cuts at: 4455 6027 4455 Size: 1572 5140 ApaLI G'TGCA_C Cuts at: 4769 5925 4769 Size: 1156 5556 ApoI r'AATT_y Cuts at: 1493 2224 2538 2878 3738 3925 3971 4021 4247 Size: 731 314 340 860 187 46 50 226 Cuts at: 4247 4399 6508 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 860 731 340 314 226 187 152 50 46 AseI AT'TA_AT Cuts at: 160 1032 5266 160 Size: 872 4234 1606 Fragments arranged by size: 4234 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 AvaI C'yCGr_G Cuts at: 1870 1943 4037 1870 Size: 73 2094 4545 Fragments arranged by size: 4545 2094 73 BamHI G'GATC_C Cuts at: 4042 4409 4042 Size: 367 6345 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2041 2068 2231 2498 4033 Size: 325 217 627 254 27 163 267 1535 Cuts at: 4033 5488 618 Size: 1455 1842 Fragments arranged by size: 1842 1535 1455 627 325 267 254 217 163 27 BanII G_rGCy'C Cuts at: 1391 2187 2298 2713 4031 1391 Size: 796 111 415 1318 4072 Fragments arranged by size: 4072 1318 796 415 111 BbeI G_GCGC'C Cuts at: 2072 2502 2072 Size: 430 6282 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2763 2784 3038 3525 928 Size: 971 864 21 254 487 4115 Fragments arranged by size: 4115 971 864 487 254 21 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4903 4937 1903 Size: 34 2966 34 3678 Fragments arranged by size: 3678 2966 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2333 4604 5814 2333 Size: 2271 1210 3231 Fragments arranged by size: 3231 2271 1210 BclI T'GATC_A Cuts at: 1548 3481 1548 Size: 1933 4779 BfrBI ATG'CAT Cuts at: 2308 3590 2308 Size: 1282 5430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5323 136 Size: 107 122 71 631 605 3651 1525 Fragments arranged by size: 3651 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6707 6707 Size: 6712 BlpI GC'TnA_GC Cuts at: 4129 4129 Size: 6712 Bme1580I G_kGCm'C Cuts at: 1790 2046 2958 4773 5929 1790 Size: 256 912 1815 1156 2573 Fragments arranged by size: 2573 1815 1156 912 256 BmgBI CAC'GTC Cuts at: 1318 3818 1318 Size: 2500 4212 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2763 4443 5401 Size: 416 53 83 399 24 1346 1680 958 Cuts at: 5401 442 Size: 1753 Fragments arranged by size: 1753 1680 1346 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6712 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2671 2671 Size: 6712 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2594 5372 1239 Size: 1355 2778 2579 Fragments arranged by size: 2778 2579 1355 Bpu10I CC'TnA_GC Cuts at: 3085 3085 Size: 6712 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4837 5702 6000 4837 Size: 865 298 5549 Fragments arranged by size: 5549 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2612 3559 3656 5375 882 Size: 617 1113 947 97 1719 2219 Fragments arranged by size: 2219 1719 1113 947 617 97 BsaAI yAC'GTr Cuts at: 493 1204 2086 2962 3067 3576 4503 493 Size: 711 882 876 105 509 927 2702 Fragments arranged by size: 2702 927 882 876 711 509 105 BsaBI GATnn'nnATC Cuts at: 6706 6706 Size: 6712 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2069 2499 4519 4901 Size: 53 83 186 936 536 430 2020 382 Cuts at: 4901 5536 275 Size: 635 1451 Fragments arranged by size: 2020 1451 936 635 536 430 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5145 5543 5817 5964 6375 5145 Size: 398 274 147 411 5482 Fragments arranged by size: 5482 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6487 Fragments arranged by size: 6487 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6712 BseYI C'CCAG_C Cuts at: 2107 2425 2817 3405 3942 5915 6411 2107 Size: 318 392 588 537 1973 496 2408 Fragments arranged by size: 2408 1973 588 537 496 392 318 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2829 1163 Size: 573 1093 5046 Fragments arranged by size: 5046 1093 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 4162 4923 5951 1540 Size: 275 186 15 2146 761 1028 2301 Fragments arranged by size: 2301 2146 1028 761 275 186 15 BsiHKAI G_wGCw'C Cuts at: 2091 4773 4858 5929 2091 Size: 2682 85 1071 2874 Fragments arranged by size: 2874 2682 1071 85 BsiWI C'GTAC_G Cuts at: 2082 2082 Size: 6712 BsmI GAATG_Cn' Cuts at: 4223 4316 4223 Size: 93 6619 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2091 2187 2298 2713 2958 4031 Size: 399 256 45 96 111 415 245 1073 Cuts at: 4031 4773 4858 5929 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1073 1071 742 415 399 256 245 111 96 85 45 BspEI T'CCGG_A Cuts at: 6375 6375 Size: 6712 BspHI T'CATG_A Cuts at: 4601 6331 4601 Size: 1730 4982 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4067 844 Size: 3223 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4599 1394 Size: 429 2776 3507 Fragments arranged by size: 3507 2776 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3687 5207 5381 65 Size: 1183 2439 1520 174 1396 Fragments arranged by size: 2439 1520 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5356 5543 1700 Size: 110 3546 187 2869 Fragments arranged by size: 3546 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6712 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6712 BssSI C'ACGA_G Cuts at: 4772 5784 4772 Size: 1012 5700 BstAPI GCAn_nnn'nTGC Cuts at: 794 2741 794 Size: 1947 4765 BstBI TT'CG_AA Cuts at: 5516 5516 Size: 6712 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6712 BstYI r'GATC_y Cuts at: 1972 3200 3764 4042 4409 4794 4811 6263 6349 Size: 1228 564 278 367 385 17 1452 86 Cuts at: 6349 6361 6707 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1228 564 385 367 346 278 86 17 12 BstZ17I GTA'TAC Cuts at: 3237 3846 3237 Size: 609 6103 BtgI C'CryG_G Cuts at: 513 1065 2016 2037 2405 2945 513 Size: 552 951 21 368 540 4280 Fragments arranged by size: 4280 951 552 540 368 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3321 487 Size: 683 56 2095 3878 Fragments arranged by size: 3878 2095 683 56 BtsI GCAGTG_nn' Cuts at: 4228 5022 5042 4228 Size: 794 20 5898 Fragments arranged by size: 5898 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6712 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6677 DraI TTT'AAA Cuts at: 2250 2793 2832 4016 2250 Size: 543 39 1184 4946 Fragments arranged by size: 4946 1184 543 39 DraIII CAC_nnn'GTG Cuts at: 2962 2962 Size: 6712 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5719 809 Size: 507 4403 1802 Fragments arranged by size: 4403 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 3040 3143 Size: 54 1425 50 275 18 183 1027 103 Cuts at: 3143 4159 5048 6677 8 Size: 1016 889 1629 43 Fragments arranged by size: 1629 1425 1027 1016 889 275 183 103 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2013 4159 1537 Size: 275 201 2146 4090 Fragments arranged by size: 4090 2146 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 2366 2690 3691 4642 1353 Size: 1013 324 1001 951 3423 Fragments arranged by size: 3423 1013 1001 951 324 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5299 5673 5819 387 Size: 1064 138 3710 374 146 1280 Fragments arranged by size: 3710 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2444 2594 2796 3050 4775 5372 6159 Size: 393 812 150 202 254 1725 597 787 Cuts at: 6159 6427 1239 Size: 268 1524 Fragments arranged by size: 1725 1524 812 787 597 393 268 254 202 150 EcoNI CCTnn'n_nnAGG Cuts at: 3672 3672 Size: 6712 EcoRI G'AATT_C Cuts at: 4021 4399 4021 Size: 378 6334 FspI TGC'GCA Cuts at: 5218 5218 Size: 6712 HaeII r_GCGC'y Cuts at: 1678 2072 2502 5859 1678 Size: 394 430 3357 2531 Fragments arranged by size: 3357 2531 430 394 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2607 2639 5353 5385 5427 Size: 32 135 32 1035 32 2714 32 42 Cuts at: 5427 5459 1373 Size: 32 2626 Fragments arranged by size: 2714 2626 1035 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2751 3858 4056 4302 669 Size: 988 281 813 1107 198 246 3079 Fragments arranged by size: 3079 1107 988 813 281 246 198 HindIII A'AGCT_T Cuts at: 748 2762 748 Size: 2014 4698 HpaI GTT'AAC Cuts at: 3858 4302 3858 Size: 444 6268 KasI G'GCGC_C Cuts at: 2068 2498 2068 Size: 430 6282 KpnI G_GTAC'C Cuts at: 4037 4037 Size: 6712 MfeI C'AATT_G Cuts at: 3951 4311 3951 Size: 360 6352 MluI A'CGCG_T Cuts at: 4009 6588 4009 Size: 2579 4133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2124 3618 5826 6010 1305 Size: 819 1494 2208 184 2007 Fragments arranged by size: 2208 2007 1494 819 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3042 10 Size: 54 1425 343 1210 3680 Fragments arranged by size: 3680 1425 1210 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3336 3920 4670 5029 5188 518 Size: 648 2170 584 750 359 159 2042 Fragments arranged by size: 2170 2042 750 648 584 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 2039 2142 2464 3280 4128 4805 5953 Size: 412 21 103 322 816 848 677 1148 Cuts at: 5953 6198 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 848 816 677 412 322 245 103 21 NarI GG'CG_CC Cuts at: 2069 2499 2069 Size: 430 6282 NcoI C'CATG_G Cuts at: 513 1065 2405 2945 513 Size: 552 1340 540 4280 Fragments arranged by size: 4280 1340 552 540 NdeI CA'TA_TG Cuts at: 387 387 Size: 6712 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6712 NotI GC'GGCC_GC Cuts at: 2013 4159 2013 Size: 2146 4566 NsiI A_TGCA'T Cuts at: 2310 3592 2310 Size: 1282 5430 NspI r_CATG'y Cuts at: 1132 1229 2308 3889 4070 5615 1132 Size: 97 1079 1581 181 1545 2229 Fragments arranged by size: 2229 1581 1545 1079 181 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6712 PciI A'CATG_T Cuts at: 5611 5611 Size: 6712 PflMI CCAn_nnn'nTGG Cuts at: 1108 2518 2951 3576 3938 1108 Size: 1410 433 625 362 3882 Fragments arranged by size: 3882 1410 625 433 362 PfoI T'CCnGG_A Cuts at: 2719 2719 Size: 6712 PmeI GTTT'AAAC Cuts at: 4016 4016 Size: 6712 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4781 4813 6294 6326 4781 Size: 32 1481 32 5167 Fragments arranged by size: 5167 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6712 PsiI TTA'TAA Cuts at: 4282 4282 Size: 6712 PstI C_TGCA'G Cuts at: 830 4064 830 Size: 3234 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6712 PvuII CAG'CTG Cuts at: 2464 3280 2464 Size: 816 5896 SacII CC_GC'GG Cuts at: 2019 2040 2019 Size: 21 6691 SalI G'TCGA_C Cuts at: 1936 4054 1936 Size: 2118 4594 SbfI CC_TGCA'GG Cuts at: 4064 4064 Size: 6712 ScaI AGT'ACT Cuts at: 4960 6672 4960 Size: 1712 5000 SfcI C'TryA_G Cuts at: 826 1045 3224 4060 5195 5876 6067 826 Size: 219 2179 836 1135 681 191 1471 Fragments arranged by size: 2179 1471 1135 836 681 219 191 SfoI GGC'GCC Cuts at: 2070 2500 2070 Size: 430 6282 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6712 SmaI CCC'GGG Cuts at: 1872 4039 1872 Size: 2167 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3194 4816 5717 5979 820 Size: 197 926 1251 1622 901 262 1553 Fragments arranged by size: 1622 1553 1251 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3067 4503 493 Size: 2574 1436 2702 Fragments arranged by size: 2702 2574 1436 SpeI A'CTAG_T Cuts at: 152 152 Size: 6712 SphI G_CATG'C Cuts at: 1132 3889 4070 1132 Size: 2757 181 3774 Fragments arranged by size: 3774 2757 181 SspI AAT'ATT Cuts at: 5 52 2386 3503 3514 4636 5 Size: 47 2334 1117 11 1122 2081 Fragments arranged by size: 2334 2081 1122 1117 47 11 StuI AGG'CCT Cuts at: 1517 1517 Size: 6712 StyI C'CwwG_G Cuts at: 513 1065 2405 2945 4151 513 Size: 552 1340 540 1206 3074 Fragments arranged by size: 3074 1340 1206 552 540 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2795 4739 4756 4909 5094 5539 325 Size: 1255 1215 1944 17 153 185 445 1498 Fragments arranged by size: 1944 1498 1255 1215 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4958 6670 96 Size: 274 80 33 51 1443 2981 1712 138 Fragments arranged by size: 2981 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6672 6704 6672 Size: 32 6680 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6712 XbaI T'CTAG_A Cuts at: 4048 4048 Size: 6712 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3939 3939 Size: 6712 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6712 XmaI C'CCGG_G Cuts at: 1870 4037 1870 Size: 2167 4545 XmnI GAAnn'nnTTC Cuts at: 2330 2728 3264 4841 2330 Size: 398 536 1577 4201 Fragments arranged by size: 4201 1577 536 398 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4520 276 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BsmBI BstXI Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI