(Linear) (Six-base) MAPSORT of: KIEE4554.seq Check: 4043 from: 1 to: 2007 KIEE4554.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AccI GT'mk_AC Cuts at: 0 1231 1840 2007 Size: 1231 609 167 Fragments arranged by size: 1231 609 167 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 791 1045 2007 Size: 439 352 254 962 Fragments arranged by size: 962 439 352 254 AflII C'TTAA_G Cuts at: 0 1189 2007 Size: 1189 818 AflIII A'CryG_T Cuts at: 0 1812 2007 Size: 1812 195 ApoI r'AATT_y Cuts at: 0 219 533 873 1733 1920 1966 2007 Size: 219 314 340 860 187 46 41 Fragments arranged by size: 860 340 314 219 187 46 41 BanI G'GyrC_C Cuts at: 0 36 63 226 493 2007 Size: 36 27 163 267 1514 Fragments arranged by size: 1514 267 163 36 27 BanII G_rGCy'C Cuts at: 0 182 293 708 2007 Size: 182 111 415 1299 Fragments arranged by size: 1299 415 182 111 BbeI G_GCGC'C Cuts at: 0 67 497 2007 Size: 67 430 1510 Fragments arranged by size: 1510 430 67 BbsI GAAGACnn'nnnn_ Cuts at: 0 758 779 1033 1520 2007 Size: 758 21 254 487 487 Fragments arranged by size: 758 487 487 254 21 BciVI GTATCCnnnnn_n' Cuts at: 0 328 2007 Size: 328 1679 BclI T'GATC_A Cuts at: 0 1476 2007 Size: 1476 531 BfrBI ATG'CAT Cuts at: 0 303 1585 2007 Size: 303 1282 422 Fragments arranged by size: 1282 422 303 Bme1580I G_kGCm'C Cuts at: 0 41 953 2007 Size: 41 912 1054 Fragments arranged by size: 1054 912 41 BmgBI CAC'GTC Cuts at: 0 1813 2007 Size: 1813 194 BmrI ACTGGGnnnn_n' Cuts at: 0 758 2007 Size: 758 1249 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 666 2007 Size: 666 1341 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 589 2007 Size: 589 1418 Bpu10I CC'TnA_GC Cuts at: 0 1080 2007 Size: 1080 927 BsaI GGTCTCn'nnnn_ Cuts at: 0 607 1554 1651 2007 Size: 607 947 97 356 Fragments arranged by size: 947 607 356 97 BsaAI yAC'GTr Cuts at: 0 81 957 1062 1571 2007 Size: 81 876 105 509 436 Fragments arranged by size: 876 509 436 105 81 BsaHI Gr'CG_yC Cuts at: 0 64 494 2007 Size: 64 430 1513 Fragments arranged by size: 1513 430 64 BseYI C'CCAG_C Cuts at: 0 102 420 812 1400 1937 2007 Size: 102 318 392 588 537 70 Fragments arranged by size: 588 537 392 318 102 70 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 824 2007 Size: 824 1183 BsiEI CG_ry'CG Cuts at: 0 11 2007 Size: 11 1996 BsiHKAI G_wGCw'C Cuts at: 0 86 2007 Size: 86 1921 BsiWI C'GTAC_G Cuts at: 0 77 2007 Size: 77 1930 Bsp1286I G_dGCh'C Cuts at: 0 41 86 182 293 708 953 2007 Size: 41 45 96 111 415 245 1054 Fragments arranged by size: 1054 415 245 111 96 45 41 BsrDI GCAATG_nn' Cuts at: 0 1682 2007 Size: 1682 325 BstAPI GCAn_nnn'nTGC Cuts at: 0 736 2007 Size: 736 1271 BstYI r'GATC_y Cuts at: 0 1195 1759 2007 Size: 1195 564 248 Fragments arranged by size: 1195 564 248 BstZ17I GTA'TAC Cuts at: 0 1232 1841 2007 Size: 1232 609 166 Fragments arranged by size: 1232 609 166 BtgI C'CryG_G Cuts at: 0 11 32 400 940 2007 Size: 11 21 368 540 1067 Fragments arranged by size: 1067 540 368 21 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1316 2007 Size: 1316 691 DraI TTT'AAA Cuts at: 0 245 788 827 2007 Size: 245 543 39 1180 Fragments arranged by size: 1180 543 245 39 DraIII CAC_nnn'GTG Cuts at: 0 957 2007 Size: 957 1050 EaeI y'GGCC_r Cuts at: 0 8 1035 1138 2007 Size: 8 1027 103 869 Fragments arranged by size: 1027 869 103 8 EagI C'GGCC_G Cuts at: 0 8 2007 Size: 8 1999 EarI CTCTTCn'nnn_ Cuts at: 0 361 685 1686 2007 Size: 361 324 1001 321 Fragments arranged by size: 1001 361 324 321 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 589 791 1045 2007 Size: 439 150 202 254 962 Fragments arranged by size: 962 439 254 202 150 EcoNI CCTnn'n_nnAGG Cuts at: 0 1667 2007 Size: 1667 340 HaeII r_GCGC'y Cuts at: 0 67 497 2007 Size: 67 430 1510 Fragments arranged by size: 1510 430 67 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 602 634 2007 Size: 602 32 1373 Fragments arranged by size: 1373 602 32 HincII GTy'rAC Cuts at: 0 746 1853 2007 Size: 746 1107 154 Fragments arranged by size: 1107 746 154 HindIII A'AGCT_T Cuts at: 0 757 2007 Size: 757 1250 HpaI GTT'AAC Cuts at: 0 1853 2007 Size: 1853 154 KasI G'GCGC_C Cuts at: 0 63 493 2007 Size: 63 430 1514 Fragments arranged by size: 1514 430 63 MfeI C'AATT_G Cuts at: 0 1946 2007 Size: 1946 61 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 119 1613 2007 Size: 119 1494 394 Fragments arranged by size: 1494 394 119 MscI TGG'CCA Cuts at: 0 1037 2007 Size: 1037 970 MslI CAynn'nnrTG Cuts at: 0 1331 1915 2007 Size: 1331 584 92 Fragments arranged by size: 1331 584 92 MspA1I CmG'CkG Cuts at: 0 13 34 137 459 1275 2007 Size: 13 21 103 322 816 732 Fragments arranged by size: 816 732 322 103 21 13 NarI GG'CG_CC Cuts at: 0 64 494 2007 Size: 64 430 1513 Fragments arranged by size: 1513 430 64 NcoI C'CATG_G Cuts at: 0 400 940 2007 Size: 400 540 1067 Fragments arranged by size: 1067 540 400 NotI GC'GGCC_GC Cuts at: 0 8 2007 Size: 8 1999 NsiI A_TGCA'T Cuts at: 0 305 1587 2007 Size: 305 1282 420 Fragments arranged by size: 1282 420 305 NspI r_CATG'y Cuts at: 0 303 1884 2007 Size: 303 1581 123 Fragments arranged by size: 1581 303 123 PflMI CCAn_nnn'nTGG Cuts at: 0 513 946 1571 1933 2007 Size: 513 433 625 362 74 Fragments arranged by size: 625 513 433 362 74 PfoI T'CCnGG_A Cuts at: 0 714 2007 Size: 714 1293 PvuII CAG'CTG Cuts at: 0 459 1275 2007 Size: 459 816 732 Fragments arranged by size: 816 732 459 SacII CC_GC'GG Cuts at: 0 14 35 2007 Size: 14 21 1972 Fragments arranged by size: 1972 21 14 SfcI C'TryA_G Cuts at: 0 1219 2007 Size: 1219 788 SfoI GGC'GCC Cuts at: 0 65 495 2007 Size: 65 430 1512 Fragments arranged by size: 1512 430 65 SmlI C'TyrA_G Cuts at: 0 1189 2007 Size: 1189 818 SnaBI TAC'GTA Cuts at: 0 1062 2007 Size: 1062 945 SphI G_CATG'C Cuts at: 0 1884 2007 Size: 1884 123 SspI AAT'ATT Cuts at: 0 381 1498 1509 2007 Size: 381 1117 11 498 Fragments arranged by size: 1117 498 381 11 StyI C'CwwG_G Cuts at: 0 400 940 2007 Size: 400 540 1067 Fragments arranged by size: 1067 540 400 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 790 2007 Size: 790 1217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1934 2007 Size: 1934 73 XmnI GAAnn'nnTTC Cuts at: 0 325 723 1259 2007 Size: 325 398 536 748 Fragments arranged by size: 748 536 398 325 Enzymes that do cut: AccI AcuI AflII AflIII ApoI BanI BanII BbeI BbsI BciVI BclI BfrBI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BsrDI BstAPI BstYI BstZ17I BtgI BtgZI DraI DraIII EaeI EagI EarI Eco57MI EcoNI HaeII Hin4I HincII HindIII HpaI KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NotI NsiI NspI PflMI PfoI PvuII SacII SfcI SfoI SmlI SnaBI SphI SspI StyI TaqII XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BglI BglII BlpI BmtI BpuEI BsaBI BsaWI BsaXI BseRI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI Bsu36I BtsI ClaI CspCI DrdI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI KpnI MluI NaeI NdeI NgoMIV NheI NruI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SpeI SrfI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI ZraI