################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AccI AccII AccIII AclI AcsI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 250 GACGTC GACGT^C Acc65I 647 GGTACC G^GTACC AhdI 502 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 647 GGTACC G^GTACC AspEI 502 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 49 GAGCTC G[AT]GC[AT]^C AvaI 40 CCCGAG C^[CT]CG[GA]G BalI 593 TGGCCA TGG^CCA BanI 647 GGTACC G^G[CT][GA]CC BanII 49 GAGCTC G[GA]GC[CT]^C BbvCI 741 CCTCAGC CCTCAGC(-5/-2) BfaI 196 CTAG C^TAG BmyI 49 GAGCTC G[AGT]GC[ACT]^C BpmI 274 CTGGAG CTGGAG(16/14) BsaI 280 GAGACC GGTCTC(1/5) BsiHKAI 49 GAGCTC G[AT]GC[AT]^C BsmAI 280 GAGAC GTCTC(1/5) BsoBI 40 CCCGAG C^[CT]CG[GA]G Bsp1286I 49 GAGCTC G[AGT]GC[ACT]^C BspDI 70 ATCGAT AT^CGAT BspHI 116 TCATGA T^CATGA BspMI 185 ACCTGC ACCTGC(4/8) BsrI 309 ACTGG ACTGG(1/-1) BstYI 162 GGATCT [GA]^GATC[CT] ClaI 70 ATCGAT AT^CGAT EaeI 593 TGGCCA [CT]^GGCC[GA] Eam1105I 502 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 112 CTCTTC CTCTTC(1/4) EcoNI 420 CCTAACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 616 GATATC GAT^ATC EcoT14I 425 CCAAGG C^C[AT][AT]GG HaeII 107 AGCGCC [GA]GCGC^[CT] HgaI 481 GACGC GACGC(5/10) KpnI 647 GGTACC GGTAC^C Ksp632I 112 CTCTTC CTCTTC(1/4) MaeI 196 CTAG C^TAG MaeIII 151 GTCAC ^GT[ACGT]AC MflI 162 GGATCT [GA]^GATC[CT] MluNI 593 TGGCCA TGG^CCA MscI 593 TGGCCA TGG^CCA MslI 153 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 724 GCTGATGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PshAI 246 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 116 TCATGA T^CATGA SacI 49 GAGCTC GAGCT^C SmlI 652 CTCAAG C^T[CT][GA]AG StyI 425 CCAAGG C^C[AT][AT]GG Tsp45I 151 GTCAC ^GT[GC]AC Tsp509I 440 AATT ^AATT XcmI 569 CCAAGCTCCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 162 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 250 GACGTC G[GA]^CG[CT]C 481 GACGCC AfaI 300 GTAC GT^AC 648 GTAC AvaII 36 GGACC G^G[AT]CC 509 GGTCC BbsI 243 GAAGAC GAAGAC(2/6) 499 GAAGAC BpuAI 243 GAAGAC GAAGAC(2/6) 499 GAAGAC BsaHI 250 GACGTC G[GA]^CG[CT]C 481 GACGCC BsgI 187 CTGCAC GTGCAG(16/14) 692 GTGCAG CfoI 108 GCGC GCG^C 516 GCGC Cfr13I 36 GGACC G^G[ACGT]CC 509 GGTCC DraII 35 GGGACCC [GA]G^G[ACGT]CC[CT] 508 GGGTCCC EcoO109I 35 GGGACCC [GA]G^G[ACGT]CC[CT] 508 GGGTCCC FauI 213 CCCGC CCCGC(4/6) 340 GCGGG HhaI 108 GCGC GCG^C 516 GCGC Hin1I 250 GACGTC G[GA]^CG[CT]C 481 GACGCC HinP1I 108 GCGC G^CGC 516 GCGC HphI 152 TCACC GGTGA(8/7) 680 TCACC MaeII 239 ACGT A^CGT 251 ACGT NlaIII 117 CATG CATG^ 577 CATG PleI 7 GACTC GAGTC(4/5) 137 GAGTC PpuMI 35 GGGACCC [GA]G^G[AT]CC[CT] 508 GGGTCCC PvuII 179 CAGCTG CAG^CTG 695 CAGCTG RsaI 300 GTAC GT^AC 648 GTAC Sau96I 36 GGACC G^G[ACGT]CC 509 GGTCC SfaNI 83 GCATC GCATC(5/9) 344 GCATC StuI 417 AGGCCT AGG^CCT 636 AGGCCT TfiI 68 GAATC G^A[AT]TC 328 GAATC TspRI 156 CACTG CA[GC]TG[ACGT][ACGT]^ 702 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AluI AlwI BbvI BcnI BsaJI BseRI BsiYI BslI BsmFI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboI MboII MnlI MspA1I MspI MvaI NciI NdeII NlaIV Sau3AI ScrFI TaqI TseI TthHB8I