(Circular) (Six-base) MAPSORT of: pFN21AE5206.seq Check: 9519 from: 1 to: 5458 pFN21AE5206 5458 bp With 182 enzymes: SgfI * January 30, 2014 17:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2259 3268 278 Size: 53 83 186 936 723 1009 2468 Fragments arranged by size: 2468 1009 936 723 186 83 53 AccI GT'mk_AC Cuts at: 1937 2801 1937 Size: 864 4594 Acc65I G'GTAC_C Cuts at: 2652 2779 2652 Size: 127 5331 AclI AA'CG_TT Cuts at: 1563 3585 3958 1563 Size: 2022 373 3063 Fragments arranged by size: 3063 2022 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3521 4905 5173 1632 Size: 1889 1384 268 1917 Fragments arranged by size: 1917 1889 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5261 AflIII A'CryG_T Cuts at: 1203 4357 5334 1203 Size: 3154 977 1327 Fragments arranged by size: 3154 1327 977 AgeI A'CCGG_T Cuts at: 4289 4289 Size: 5458 AhdI GACnn_n'nnGTC Cuts at: 2512 4187 2512 Size: 1675 3783 AleI CACnn'nnGTG Cuts at: 1166 2162 1166 Size: 996 4462 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2421 2585 1184 Size: 1237 164 4057 Fragments arranged by size: 4057 1237 164 AlwNI CAG_nnn'CTG Cuts at: 3201 4773 3201 Size: 1572 3886 ApaLI G'TGCA_C Cuts at: 3515 4671 3515 Size: 1156 4302 ApoI r'AATT_y Cuts at: 1493 2767 2993 3145 5254 1493 Size: 1274 226 152 2109 1697 Fragments arranged by size: 2109 1697 1274 226 152 AseI AT'TA_AT Cuts at: 160 1032 4012 160 Size: 872 2980 1606 Fragments arranged by size: 2980 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 AvaI C'yCGr_G Cuts at: 1870 1943 2045 2783 1870 Size: 73 102 738 4545 Fragments arranged by size: 4545 738 102 73 BamHI G'GATC_C Cuts at: 2788 3155 2788 Size: 367 5091 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2652 2779 4234 618 Size: 325 217 627 865 127 1455 1842 Fragments arranged by size: 1842 1455 865 627 325 217 127 BanII G_rGCy'C Cuts at: 1391 2058 2777 1391 Size: 667 719 4072 Fragments arranged by size: 4072 719 667 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2255 2511 928 Size: 971 356 256 3875 Fragments arranged by size: 3875 971 356 256 BbvCI CC'TCA_GC Cuts at: 2747 2747 Size: 5458 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3649 3683 1903 Size: 34 1712 34 3678 Fragments arranged by size: 3678 1712 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3350 4560 3350 Size: 1210 4248 BclI T'GATC_A Cuts at: 1548 1548 Size: 5458 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4069 136 Size: 107 122 71 631 605 2397 1525 Fragments arranged by size: 2397 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5453 5453 Size: 5458 BlpI GC'TnA_GC Cuts at: 2875 2875 Size: 5458 Bme1580I G_kGCm'C Cuts at: 1790 3519 4675 1790 Size: 1729 1156 2573 Fragments arranged by size: 2573 1729 1156 BmgBI CAC'GTC Cuts at: 1318 2245 1318 Size: 927 4531 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3189 4147 442 Size: 416 53 83 399 24 1772 958 1753 Fragments arranged by size: 1772 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5458 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 4118 1239 Size: 1061 1818 2579 Fragments arranged by size: 2579 1818 1061 Bpu10I CC'TnA_GC Cuts at: 2747 2747 Size: 5458 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2642 3583 4448 4746 2642 Size: 941 865 298 3354 Fragments arranged by size: 3354 941 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2279 4121 882 Size: 617 780 1842 2219 Fragments arranged by size: 2219 1842 780 617 BsaAI yAC'GTr Cuts at: 493 1204 3249 493 Size: 711 2045 2702 Fragments arranged by size: 2702 2045 711 BsaBI GATnn'nnATC Cuts at: 5452 5452 Size: 5458 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2256 2487 3265 3647 Size: 53 83 186 936 723 231 778 382 Cuts at: 3647 4282 275 Size: 635 1451 Fragments arranged by size: 1451 936 778 723 635 382 231 186 83 53 BsaWI w'CCGG_w Cuts at: 3891 4289 4563 4710 5121 3891 Size: 398 274 147 411 4228 Fragments arranged by size: 4228 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5233 Fragments arranged by size: 5233 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 2297 2492 1186 Size: 1099 12 195 4152 Fragments arranged by size: 4152 1099 195 12 BseYI C'CCAG_C Cuts at: 4661 5157 4661 Size: 496 4962 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2177 2718 1163 Size: 573 441 541 3903 Fragments arranged by size: 3903 573 541 441 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2908 3669 4697 1540 Size: 275 186 907 761 1028 2301 Fragments arranged by size: 2301 1028 907 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2058 3519 3604 4675 2058 Size: 1461 85 1071 2841 Fragments arranged by size: 2841 1461 1071 85 BsmI GAATG_Cn' Cuts at: 2969 3062 2969 Size: 93 5365 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2777 3519 3604 4675 1391 Size: 399 268 719 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 719 399 268 85 BspEI T'CCGG_A Cuts at: 5121 5121 Size: 5458 BspHI T'CATG_A Cuts at: 2121 3347 5077 2121 Size: 1226 1730 2502 Fragments arranged by size: 2502 1730 1226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2199 2813 844 Size: 1355 614 3489 Fragments arranged by size: 3489 1355 614 BsrBI CCG'CTC Cuts at: 1394 1823 3345 1394 Size: 429 1522 3507 Fragments arranged by size: 3507 1522 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3953 4127 65 Size: 1183 2705 174 1396 Fragments arranged by size: 2705 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4102 4289 1700 Size: 110 2292 187 2869 Fragments arranged by size: 2869 2292 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5458 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5458 BssSI C'ACGA_G Cuts at: 3518 4530 3518 Size: 1012 4446 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5458 BstBI TT'CG_AA Cuts at: 4262 4262 Size: 5458 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5458 BstYI r'GATC_y Cuts at: 1972 2167 2788 3155 3540 3557 5009 5095 5107 Size: 195 621 367 385 17 1452 86 12 Cuts at: 5107 5453 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 621 385 367 346 195 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4906 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4719 Fragments arranged by size: 4719 683 56 BtsI GCAGTG_nn' Cuts at: 2974 3768 3788 2974 Size: 794 20 4644 Fragments arranged by size: 4644 794 20 ClaI AT'CG_AT Cuts at: 1554 2076 1554 Size: 522 4936 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2479 2514 529 Size: 35 1915 35 3473 Fragments arranged by size: 3473 1915 35 35 DraI TTT'AAA Cuts at: 2762 2762 Size: 5458 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4465 809 Size: 507 3149 1802 Fragments arranged by size: 3149 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2598 2905 3794 Size: 54 1425 50 275 18 768 307 889 Cuts at: 3794 5423 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 768 307 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2905 1537 Size: 275 1093 4090 Fragments arranged by size: 4090 1093 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2123 3388 1353 Size: 770 1265 3423 Fragments arranged by size: 3423 1265 770 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4045 4419 4565 387 Size: 1064 138 2456 374 146 1280 Fragments arranged by size: 2456 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2056 2056 Size: 5458 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 3521 4118 4905 5173 1239 Size: 393 668 1221 597 787 268 1524 Fragments arranged by size: 1524 1221 787 668 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2429 2429 Size: 5458 EcoO109I rG'GnC_Cy Cuts at: 2041 2514 2041 Size: 473 4985 EcoRI G'AATT_C Cuts at: 2767 3145 2767 Size: 378 5080 EcoRV GAT'ATC Cuts at: 2623 2623 Size: 5458 FspI TGC'GCA Cuts at: 3964 3964 Size: 5458 HaeII r_GCGC'y Cuts at: 1678 2116 4605 1678 Size: 438 2489 2531 Fragments arranged by size: 2531 2489 438 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2073 2105 2498 2530 4099 Size: 32 135 32 501 32 393 32 1569 Cuts at: 4099 4131 4173 4205 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1569 501 393 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2802 3048 669 Size: 988 281 864 246 3079 Fragments arranged by size: 3079 988 864 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5458 HpaI GTT'AAC Cuts at: 3048 3048 Size: 5458 KpnI G_GTAC'C Cuts at: 2656 2783 2656 Size: 127 5331 MfeI C'AATT_G Cuts at: 3057 3057 Size: 5458 MluI A'CGCG_T Cuts at: 5334 5334 Size: 5458 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2361 4572 4756 1305 Size: 1056 2211 184 2007 Fragments arranged by size: 2211 2007 1056 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2600 10 Size: 54 1425 343 768 2868 Fragments arranged by size: 2868 1425 768 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2162 3416 3775 3934 518 Size: 648 996 1254 359 159 2042 Fragments arranged by size: 2042 1254 996 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2186 2345 2702 2874 3551 4699 4944 1606 Size: 580 159 357 172 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 580 357 245 172 159 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4906 NdeI CA'TA_TG Cuts at: 387 387 Size: 5458 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5458 NotI GC'GGCC_GC Cuts at: 2905 2905 Size: 5458 NspI r_CATG'y Cuts at: 1132 1229 2816 4361 1132 Size: 97 1587 1545 2229 Fragments arranged by size: 2229 1587 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5458 PciI A'CATG_T Cuts at: 4357 4357 Size: 5458 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5458 PfoI T'CCnGG_A Cuts at: 2277 2529 2277 Size: 252 5206 PmeI GTTT'AAAC Cuts at: 2762 2762 Size: 5458 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3527 3559 5040 5072 3527 Size: 32 1481 32 3913 Fragments arranged by size: 3913 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2041 2514 2041 Size: 473 4985 PshAI GACnn'nnGTC Cuts at: 1613 2255 1613 Size: 642 4816 PsiI TTA'TAA Cuts at: 3028 3028 Size: 5458 PstI C_TGCA'G Cuts at: 830 2810 830 Size: 1980 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5458 PvuII CAG'CTG Cuts at: 2186 2702 2186 Size: 516 4942 SacI G_AGCT'C Cuts at: 2058 2058 Size: 5458 SalI G'TCGA_C Cuts at: 1936 2800 1936 Size: 864 4594 SanDI GG'GwC_CC Cuts at: 2041 2514 2041 Size: 473 4985 SbfI CC_TGCA'GG Cuts at: 2810 2810 Size: 5458 ScaI AGT'ACT Cuts at: 3706 5418 3706 Size: 1712 3746 SfcI C'TryA_G Cuts at: 826 1045 2806 3941 4622 4813 826 Size: 219 1761 1135 681 191 1471 Fragments arranged by size: 1761 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5458 SmaI CCC'GGG Cuts at: 1872 2785 1872 Size: 913 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2657 3562 4463 4725 820 Size: 197 926 714 905 901 262 1553 Fragments arranged by size: 1553 926 905 901 714 262 197 SnaBI TAC'GTA Cuts at: 493 3249 493 Size: 2756 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5458 SphI G_CATG'C Cuts at: 1132 2816 1132 Size: 1684 3774 SspI AAT'ATT Cuts at: 5 52 3382 5 Size: 47 3330 2081 Fragments arranged by size: 3330 2081 47 StuI AGG'CCT Cuts at: 1517 2424 2643 1517 Size: 907 219 4332 Fragments arranged by size: 4332 907 219 StyI C'CwwG_G Cuts at: 513 1065 2430 2897 513 Size: 552 1365 467 3074 Fragments arranged by size: 3074 1365 552 467 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3485 3502 3655 3840 4285 325 Size: 1255 1905 17 153 185 445 1498 Fragments arranged by size: 1905 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3704 5416 96 Size: 274 80 33 51 1443 1727 1712 138 Fragments arranged by size: 1727 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2318 2350 5418 5450 2318 Size: 32 3068 32 2326 Fragments arranged by size: 3068 2326 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5458 XbaI T'CTAG_A Cuts at: 2794 2794 Size: 5458 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2581 2581 Size: 5458 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5458 XmaI C'CCGG_G Cuts at: 1870 2783 1870 Size: 913 4545 XmnI GAAnn'nnTTC Cuts at: 3587 3587 Size: 5458 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2257 3266 276 Size: 53 83 186 936 723 1009 2468 Fragments arranged by size: 2468 1009 936 723 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI