(Linear) (Six-base) MAPSORT of: KIEE5206.seq Check: 5766 from: 1 to: 753 KIEE5206.orf With 182 enzymes: SgfI * January 29, 2014 11:50 .. AatII G_ACGT'C Cuts at: 0 254 753 Size: 254 499 Acc65I G'GTAC_C Cuts at: 0 647 753 Size: 647 106 AhdI GACnn_n'nnGTC Cuts at: 0 507 753 Size: 507 246 AleI CACnn'nnGTG Cuts at: 0 157 753 Size: 157 596 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 416 580 753 Size: 416 164 173 Fragments arranged by size: 416 173 164 AvaI C'yCGr_G Cuts at: 0 40 753 Size: 40 713 BanI G'GyrC_C Cuts at: 0 647 753 Size: 647 106 BanII G_rGCy'C Cuts at: 0 53 753 Size: 53 700 BbsI GAAGACnn'nnnn_ Cuts at: 0 250 506 753 Size: 250 256 247 Fragments arranged by size: 256 250 247 BbvCI CC'TCA_GC Cuts at: 0 742 753 Size: 742 11 BmgBI CAC'GTC Cuts at: 0 240 753 Size: 240 513 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 753 Size: 295 458 Bpu10I CC'TnA_GC Cuts at: 0 742 753 Size: 742 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 637 753 Size: 637 116 BsaI GGTCTCn'nnnn_ Cuts at: 0 274 753 Size: 274 479 BsaHI Gr'CG_yC Cuts at: 0 251 482 753 Size: 251 231 271 Fragments arranged by size: 271 251 231 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 280 292 487 753 Size: 280 12 195 266 Fragments arranged by size: 280 266 195 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 713 753 Size: 172 541 40 Fragments arranged by size: 541 172 40 BsiHKAI G_wGCw'C Cuts at: 0 53 753 Size: 53 700 Bsp1286I G_dGCh'C Cuts at: 0 53 753 Size: 53 700 BspHI T'CATG_A Cuts at: 0 116 753 Size: 116 637 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 194 753 Size: 194 559 BstYI r'GATC_y Cuts at: 0 162 753 Size: 162 591 ClaI AT'CG_AT Cuts at: 0 71 753 Size: 71 682 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 474 509 753 Size: 474 35 244 Fragments arranged by size: 474 244 35 EaeI y'GGCC_r Cuts at: 0 593 753 Size: 593 160 EarI CTCTTCn'nnn_ Cuts at: 0 118 753 Size: 118 635 EcoICRI GAG'CTC Cuts at: 0 51 753 Size: 51 702 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 753 Size: 295 458 EcoNI CCTnn'n_nnAGG Cuts at: 0 424 753 Size: 424 329 EcoO109I rG'GnC_Cy Cuts at: 0 36 509 753 Size: 36 473 244 Fragments arranged by size: 473 244 36 EcoRV GAT'ATC Cuts at: 0 618 753 Size: 618 135 HaeII r_GCGC'y Cuts at: 0 111 753 Size: 111 642 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 68 100 493 525 753 Size: 68 32 393 32 228 Fragments arranged by size: 393 228 68 32 32 KpnI G_GTAC'C Cuts at: 0 651 753 Size: 651 102 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 356 753 Size: 356 397 MscI TGG'CCA Cuts at: 0 595 753 Size: 595 158 MslI CAynn'nnrTG Cuts at: 0 157 753 Size: 157 596 MspA1I CmG'CkG Cuts at: 0 181 340 697 753 Size: 181 159 357 56 Fragments arranged by size: 357 181 159 56 PfoI T'CCnGG_A Cuts at: 0 272 524 753 Size: 272 252 229 Fragments arranged by size: 272 252 229 PpuMI rG'GwC_Cy Cuts at: 0 36 509 753 Size: 36 473 244 Fragments arranged by size: 473 244 36 PshAI GACnn'nnGTC Cuts at: 0 250 753 Size: 250 503 PvuII CAG'CTG Cuts at: 0 181 697 753 Size: 181 516 56 Fragments arranged by size: 516 181 56 SacI G_AGCT'C Cuts at: 0 53 753 Size: 53 700 SanDI GG'GwC_CC Cuts at: 0 36 509 753 Size: 36 473 244 Fragments arranged by size: 473 244 36 SmlI C'TyrA_G Cuts at: 0 652 753 Size: 652 101 StuI AGG'CCT Cuts at: 0 419 638 753 Size: 419 219 115 Fragments arranged by size: 419 219 115 StyI C'CwwG_G Cuts at: 0 425 753 Size: 425 328 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 313 345 753 Size: 313 32 408 Fragments arranged by size: 408 313 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 576 753 Size: 576 177 ZraI GAC'GTC Cuts at: 0 252 753 Size: 252 501 Enzymes that do cut: AatII Acc65I AhdI AleI AlfI AvaI BanI BanII BbsI BbvCI BmgBI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BsgI BsiHKAI Bsp1286I BspHI BspMI BstYI ClaI CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I KpnI MmeI MscI MslI MspA1I PfoI PpuMI PshAI PvuII SacI SanDI SmlI StuI StyI TstI XcmI ZraI Enzymes that do not cut: AarI AccI AclI AcuI AfeI AflII AflIII AgeI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BsaAI BsaBI BsaWI BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EagI EciI EcoRI FalI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI