(Circular) (Six-base) MAPSORT of: pFN21AE6251.seq Check: 9579 from: 1 to: 5497 pFN21AE6251 5497 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3307 278 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2840 1937 Size: 903 4594 Acc65I G'GTAC_C Cuts at: 2818 2818 Size: 5497 AclI AA'CG_TT Cuts at: 1563 3624 3997 1563 Size: 2061 373 3063 Fragments arranged by size: 3063 2061 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3560 4944 5212 1632 Size: 1928 1384 268 1917 Fragments arranged by size: 1928 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5300 AflIII A'CryG_T Cuts at: 1203 2395 4396 5373 1203 Size: 1192 2001 977 1327 Fragments arranged by size: 2001 1327 1192 977 AgeI A'CCGG_T Cuts at: 4328 4328 Size: 5497 AhdI GACnn_n'nnGTC Cuts at: 4226 4226 Size: 5497 AleI CACnn'nnGTG Cuts at: 1166 2157 1166 Size: 991 4506 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2221 1184 Size: 1037 4460 AlwNI CAG_nnn'CTG Cuts at: 3240 4812 3240 Size: 1572 3925 ApaI G_GGCC'C Cuts at: 2654 2654 Size: 5497 ApaLI G'TGCA_C Cuts at: 3554 4710 3554 Size: 1156 4341 ApoI r'AATT_y Cuts at: 1493 2306 2806 3032 3184 5293 1493 Size: 813 500 226 152 2109 1697 Fragments arranged by size: 2109 1697 813 500 226 152 AseI AT'TA_AT Cuts at: 160 1032 4051 160 Size: 872 3019 1606 Fragments arranged by size: 3019 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 AvaI C'yCGr_G Cuts at: 1870 1943 2822 1870 Size: 73 879 4545 Fragments arranged by size: 4545 879 73 BamHI G'GATC_C Cuts at: 2827 3194 2827 Size: 367 5130 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2818 4273 618 Size: 325 217 627 1031 1455 1842 Fragments arranged by size: 1842 1455 1031 627 325 217 BanII G_rGCy'C Cuts at: 1391 2654 2816 1391 Size: 1263 162 4072 Fragments arranged by size: 4072 1263 162 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2665 928 Size: 971 766 3760 Fragments arranged by size: 3760 971 766 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2526 2560 3688 3722 1903 Size: 34 589 34 1128 34 3678 Fragments arranged by size: 3678 1128 589 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3389 4599 3389 Size: 1210 4287 BclI T'GATC_A Cuts at: 1548 1548 Size: 5497 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4108 136 Size: 107 122 71 631 605 2436 1525 Fragments arranged by size: 2436 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2435 5492 2435 Size: 3057 2440 BlpI GC'TnA_GC Cuts at: 2914 2914 Size: 5497 Bme1580I G_kGCm'C Cuts at: 1790 2654 3558 4714 1790 Size: 864 904 1156 2573 Fragments arranged by size: 2573 1156 904 864 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5497 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3228 4186 442 Size: 416 53 83 399 24 1811 958 1753 Fragments arranged by size: 1811 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5497 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 2712 4157 1239 Size: 1076 397 1445 2579 Fragments arranged by size: 2579 1445 1076 397 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2305 3622 4487 4785 2305 Size: 1317 865 298 3017 Fragments arranged by size: 3017 1317 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4160 882 Size: 617 2661 2219 Fragments arranged by size: 2661 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3288 493 Size: 711 2084 2702 Fragments arranged by size: 2702 2084 711 BsaBI GATnn'nnATC Cuts at: 5491 5491 Size: 5497 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3304 3686 4321 275 Size: 53 83 186 936 1771 382 635 1451 Fragments arranged by size: 1771 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3930 4328 4602 4749 5160 3930 Size: 398 274 147 411 4267 Fragments arranged by size: 4267 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5272 Fragments arranged by size: 5272 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5497 BseYI C'CCAG_C Cuts at: 2463 4700 5196 2463 Size: 2237 496 2764 Fragments arranged by size: 2764 2237 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2622 1163 Size: 573 886 4038 Fragments arranged by size: 4038 886 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2947 3708 4736 1540 Size: 275 186 946 761 1028 2301 Fragments arranged by size: 2301 1028 946 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3558 3643 4714 3558 Size: 85 1071 4341 Fragments arranged by size: 4341 1071 85 BsmI GAATG_Cn' Cuts at: 3008 3101 3008 Size: 93 5404 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2654 2816 3558 3643 4714 1391 Size: 399 864 162 742 85 1071 2174 Fragments arranged by size: 2174 1071 864 742 399 162 85 BspEI T'CCGG_A Cuts at: 5160 5160 Size: 5497 BspHI T'CATG_A Cuts at: 2698 3386 5116 2698 Size: 688 1730 3079 Fragments arranged by size: 3079 1730 688 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2852 844 Size: 2008 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3384 1394 Size: 429 1561 3507 Fragments arranged by size: 3507 1561 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3992 4166 65 Size: 1183 2744 174 1396 Fragments arranged by size: 2744 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4141 4328 1700 Size: 110 2331 187 2869 Fragments arranged by size: 2869 2331 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5497 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5497 BssSI C'ACGA_G Cuts at: 3557 4569 3557 Size: 1012 4485 BstAPI GCAn_nnn'nTGC Cuts at: 794 2609 794 Size: 1815 3682 BstBI TT'CG_AA Cuts at: 4301 4301 Size: 5497 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5497 BstXI CCAn_nnnn'nTGG Cuts at: 2593 2593 Size: 5497 BstYI r'GATC_y Cuts at: 1972 2335 2417 2435 2827 3194 3579 3596 5048 Size: 363 82 18 392 367 385 17 1452 Cuts at: 5048 5134 5146 5492 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 392 385 367 363 346 86 82 18 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4945 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4758 Fragments arranged by size: 4758 683 56 BtsI GCAGTG_nn' Cuts at: 2203 3013 3807 3827 2203 Size: 810 794 20 3873 Fragments arranged by size: 3873 810 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5497 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5462 DraI TTT'AAA Cuts at: 2801 2801 Size: 5497 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4504 809 Size: 507 3188 1802 Fragments arranged by size: 3188 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2944 3833 5462 Size: 54 1425 50 275 18 1114 889 1629 Cuts at: 5462 8 Size: 43 Fragments arranged by size: 1629 1425 1114 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2944 1537 Size: 275 1132 4090 Fragments arranged by size: 4090 1132 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3427 1353 Size: 2074 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2160 4084 4458 4604 387 Size: 1064 138 571 1924 374 146 1280 Fragments arranged by size: 1924 1280 1064 571 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 2712 3560 4157 4944 5212 1239 Size: 393 683 397 848 597 787 268 1524 Fragments arranged by size: 1524 848 787 683 597 397 393 268 EcoO109I rG'GnC_Cy Cuts at: 2650 2651 2650 Size: 1 5496 EcoRI G'AATT_C Cuts at: 2806 3184 2806 Size: 378 5119 EcoRV GAT'ATC Cuts at: 2353 2353 Size: 5497 FspI TGC'GCA Cuts at: 4003 4003 Size: 5497 HaeII r_GCGC'y Cuts at: 1678 4644 1678 Size: 2966 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2327 2359 4138 4170 4212 Size: 32 135 32 755 32 1779 32 42 Cuts at: 4212 4244 1373 Size: 32 2626 Fragments arranged by size: 2626 1779 755 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2742 2841 3087 669 Size: 988 281 804 99 246 3079 Fragments arranged by size: 3079 988 804 281 246 99 HindIII A'AGCT_T Cuts at: 748 2366 748 Size: 1618 3879 HpaI GTT'AAC Cuts at: 2742 3087 2742 Size: 345 5152 KpnI G_GTAC'C Cuts at: 2822 2822 Size: 5497 MfeI C'AATT_G Cuts at: 3096 3096 Size: 5497 MluI A'CGCG_T Cuts at: 5373 5373 Size: 5497 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4611 4795 1305 Size: 3306 184 2007 Fragments arranged by size: 3306 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3675 Fragments arranged by size: 3675 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2157 2703 2793 3455 3814 3973 518 Size: 648 991 546 90 662 359 159 2042 Fragments arranged by size: 2042 991 662 648 546 359 159 90 MspA1I CmG'CkG Cuts at: 1606 2913 3590 4738 4983 1606 Size: 1307 677 1148 245 2120 Fragments arranged by size: 2120 1307 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4945 NdeI CA'TA_TG Cuts at: 387 387 Size: 5497 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5497 NotI GC'GGCC_GC Cuts at: 2944 2944 Size: 5497 NspI r_CATG'y Cuts at: 1132 1229 2399 2855 4400 1132 Size: 97 1170 456 1545 2229 Fragments arranged by size: 2229 1545 1170 456 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5497 PciI A'CATG_T Cuts at: 2395 4396 2395 Size: 2001 3496 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5497 PmeI GTTT'AAAC Cuts at: 2801 2801 Size: 5497 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3566 3598 5079 5111 3566 Size: 32 1481 32 3952 Fragments arranged by size: 3952 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5497 PsiI TTA'TAA Cuts at: 3067 3067 Size: 5497 PspOMI G'GGCC_C Cuts at: 2650 2650 Size: 5497 PstI C_TGCA'G Cuts at: 830 2849 830 Size: 2019 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5497 SalI G'TCGA_C Cuts at: 1936 2839 1936 Size: 903 4594 SbfI CC_TGCA'GG Cuts at: 2849 2849 Size: 5497 ScaI AGT'ACT Cuts at: 3745 5457 3745 Size: 1712 3785 SfcI C'TryA_G Cuts at: 826 1045 2682 2845 3980 4661 4852 826 Size: 219 1637 163 1135 681 191 1471 Fragments arranged by size: 1637 1471 1135 681 219 191 163 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5497 SmaI CCC'GGG Cuts at: 1872 2824 1872 Size: 952 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2320 3601 4502 4764 820 Size: 197 926 377 1281 901 262 1553 Fragments arranged by size: 1553 1281 926 901 377 262 197 SnaBI TAC'GTA Cuts at: 493 3288 493 Size: 2795 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5497 SphI G_CATG'C Cuts at: 1132 2855 1132 Size: 1723 3774 SspI AAT'ATT Cuts at: 5 52 3421 5 Size: 47 3369 2081 Fragments arranged by size: 3369 2081 47 StuI AGG'CCT Cuts at: 1517 2279 1517 Size: 762 4735 StyI C'CwwG_G Cuts at: 513 1065 2936 513 Size: 552 1871 3074 Fragments arranged by size: 3074 1871 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2176 3524 3541 3694 3879 4324 325 Size: 1255 596 1348 17 153 185 445 1498 Fragments arranged by size: 1498 1348 1255 596 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3743 5455 96 Size: 274 80 33 51 1443 1766 1712 138 Fragments arranged by size: 1766 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2402 2434 5457 5489 2402 Size: 32 3023 32 2410 Fragments arranged by size: 3023 2410 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2594 1316 Size: 1278 4219 XbaI T'CTAG_A Cuts at: 2833 2833 Size: 5497 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2122 2290 2122 Size: 168 5329 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5497 XmaI C'CCGG_G Cuts at: 1870 2822 1870 Size: 952 4545 XmnI GAAnn'nnTTC Cuts at: 3626 3626 Size: 5497 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3305 276 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI