################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I CelII ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MroI MunI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcyI 398 GGCGCC G[GA]^CG[CT]C AfaI 463 GTAC GT^AC AocI 493 CCTCAGG CC^T[ACGT]AGG Aor51HI 446 AGCGCT AGC^GCT AvaII 516 GGACC G^G[AT]CC BalI 484 TGGCCA TGG^CCA BbeI 398 GGCGCC GGCGC^C BbsI 163 GTCTTC GAAGAC(2/6) BfaI 694 CTAG C^TAG BglII 863 AGATCT A^GATCT BpuAI 163 GTCTTC GAAGAC(2/6) BsaHI 398 GGCGCC G[GA]^CG[CT]C BsgI 533 CTGCAC GTGCAG(16/14) BsmFI 422 GGGAC GGGAC(10/14) BsrBI 1288 GAGCGG CCGCTC(-3/-3) BstEII 829 GGTGACC G^GT[ACGT]ACC BstPI 829 GGTGACC G^GT[ACGT]ACC BstYI 863 AGATCT [GA]^GATC[CT] Bsu36I 493 CCTCAGG CC^T[ACGT]AGG DrdI 148 GACGTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 446 AGCGCT AGC^GCT Eco81I 493 CCTCAGG CC^T[ACGT]AGG EcoO65I 829 GGTGACC G^GT[ACGT]ACC HgaI 579 GCGTC GACGC(5/10) Hin1I 398 GGCGCC G[GA]^CG[CT]C HinfI 991 GAATC G^A[ACGT]TC KasI 398 GGCGCC G^GCGCC KspI 198 CCGCGG CCGC^GG MaeI 694 CTAG C^TAG MflI 863 AGATCT [GA]^GATC[CT] MluNI 484 TGGCCA TGG^CCA MscI 484 TGGCCA TGG^CCA MseI 1145 TTAA T^TAA NarI 398 GGCGCC GG^CGCC NcoI 1095 CCATGG C^CATGG PflMI 98 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1071 CTGCAG CTGCA^G RsaI 463 GTAC GT^AC SacII 198 CCGCGG CCGC^GG SapI 1293 GAAGAGC GCTCTTC(1/4) SfoI 398 GGCGCC GGC^GCC TfiI 991 GAATC G^A[AT]TC Tru9I 1145 TTAA T^TAA Tsp509I 216 AATT ^AATT Van91I 98 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 863 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccII 199 CGCG CG^CG 586 CGCG BspMI 11 ACCTGC ACCTGC(4/8) 351 ACCTGC BstUI 199 CGCG CG^CG 586 CGCG DpnII 864 GATC ^GATC 1222 GATC EarI 31 CTCTTC CTCTTC(1/4) 1293 GAAGAG HaeII 398 GGCGCC [GA]GCGC^[CT] 446 AGCGCT Ksp632I 31 CTCTTC CTCTTC(1/4) 1293 GAAGAG MaeII 149 ACGT A^CGT 1049 ACGT MboI 864 GATC ^GATC 1222 GATC MslI 735 CATCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1277 CACCGTGGTG MvnI 199 CGCG CG^CG 586 CGCG NdeII 864 GATC ^GATC 1222 GATC NspI 740 ACATGC [GA]CATG^[CT] 1298 GCATGT Sau3AI 864 GATC ^GATC 1222 GATC SfcI 222 CTATAG C^T[GA][CT]AG 1071 CTGCAG XcmI 936 CCAGGGGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1167 CCAGCTCACGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AluI ApaI AspHI BanI BanII BbvI BglI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DraIII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboII MnlI MspA1I MspI MvaI MwoI NlaIII NlaIV PvuII Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I TspRI TthHB8I