(Linear) (Six-base) MAPSORT of: KIEE4420.seq Check: 8341 from: 1 to: 1305 KIEE4420.orf With 182 enzymes: SgfI * March 22, 2012 17:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 20 1305 Size: 20 1285 AfeI AGC'GCT Cuts at: 0 448 1305 Size: 448 857 AleI CACnn'nnGTG Cuts at: 0 1281 1305 Size: 1281 24 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 757 1305 Size: 757 548 ApaI G_GGCC'C Cuts at: 0 73 1037 1065 1190 1305 Size: 73 964 28 125 115 Fragments arranged by size: 964 125 115 73 28 BanI G'GyrC_C Cuts at: 0 125 238 398 553 1204 1275 1305 Size: 125 113 160 155 651 71 30 Fragments arranged by size: 651 160 155 125 113 71 30 BanII G_rGCy'C Cuts at: 0 73 1037 1065 1190 1305 Size: 73 964 28 125 115 Fragments arranged by size: 964 125 115 73 28 BbeI G_GCGC'C Cuts at: 0 402 1305 Size: 402 903 BbsI GAAGACnn'nnnn_ Cuts at: 0 156 1305 Size: 156 1149 BglI GCCn_nnn'nGGC Cuts at: 0 316 547 596 668 940 1305 Size: 316 231 49 72 272 365 Fragments arranged by size: 365 316 272 231 72 49 BglII A'GATC_T Cuts at: 0 863 1305 Size: 863 442 Bme1580I G_kGCm'C Cuts at: 0 73 241 459 576 664 681 723 763 Size: 73 168 218 117 88 17 42 40 Cuts at: 763 1037 1065 1190 1209 1305 Size: 274 28 125 19 96 Fragments arranged by size: 274 218 168 125 117 96 88 73 42 40 28 19 17 BmgBI CAC'GTC Cuts at: 0 150 1305 Size: 150 1155 BmrI ACTGGGnnnn_n' Cuts at: 0 114 272 396 429 1039 1305 Size: 114 158 124 33 610 266 Fragments arranged by size: 610 266 158 124 114 33 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 973 1305 Size: 973 332 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 544 775 928 968 1305 Size: 151 393 231 153 40 337 Fragments arranged by size: 393 337 231 153 151 40 Bpu10I CC'TnA_GC Cuts at: 0 1192 1305 Size: 1192 113 BsaHI Gr'CG_yC Cuts at: 0 399 1305 Size: 399 906 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 259 289 1305 Size: 259 30 1016 Fragments arranged by size: 1016 259 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 482 1126 1129 1305 Size: 482 644 3 176 Fragments arranged by size: 644 482 176 3 BseYI C'CCAG_C Cuts at: 0 230 234 407 564 730 968 1305 Size: 230 4 173 157 166 238 337 Fragments arranged by size: 337 238 230 173 166 157 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 518 1305 Size: 518 787 BsiHKAI G_wGCw'C Cuts at: 0 914 959 1090 1305 Size: 914 45 131 215 Fragments arranged by size: 914 215 131 45 BsmI GAATG_Cn' Cuts at: 0 114 300 805 1305 Size: 114 186 505 500 Fragments arranged by size: 505 500 186 114 Bsp1286I G_dGCh'C Cuts at: 0 73 241 459 576 664 681 723 763 Size: 73 168 218 117 88 17 42 40 Cuts at: 763 914 959 1037 1065 1090 1190 1209 1305 Size: 151 45 78 28 25 100 19 96 Fragments arranged by size: 218 168 151 117 100 96 88 78 73 45 42 40 28 25 19 17 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 20 360 1305 Size: 20 340 945 Fragments arranged by size: 945 340 20 BsrBI CCG'CTC Cuts at: 0 1290 1305 Size: 1290 15 BsrFI r'CCGG_y Cuts at: 0 86 440 652 843 1305 Size: 86 354 212 191 462 Fragments arranged by size: 462 354 212 191 86 BstEII G'GTnAC_C Cuts at: 0 829 1305 Size: 829 476 BstYI r'GATC_y Cuts at: 0 863 1305 Size: 863 442 Bsu36I CC'TnA_GG Cuts at: 0 494 1305 Size: 494 811 BtgI C'CryG_G Cuts at: 0 198 1095 1279 1305 Size: 198 897 184 26 Fragments arranged by size: 897 198 184 26 BtsI GCAGTG_nn' Cuts at: 0 574 755 1305 Size: 574 181 550 Fragments arranged by size: 574 550 181 DraIII CAC_nnn'GTG Cuts at: 0 122 144 795 1305 Size: 122 22 651 510 Fragments arranged by size: 651 510 122 22 DrdI GACnn_nn'nnGTC Cuts at: 0 154 1305 Size: 154 1151 EaeI y'GGCC_r Cuts at: 0 484 654 797 1092 1098 1305 Size: 484 170 143 295 6 207 Fragments arranged by size: 484 295 207 170 143 6 EarI CTCTTCn'nnn_ Cuts at: 0 37 1288 1305 Size: 37 1251 17 Fragments arranged by size: 1251 37 17 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1099 1305 Size: 1099 206 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 544 775 928 968 1305 Size: 151 393 231 153 40 337 Fragments arranged by size: 393 337 231 153 151 40 EcoO109I rG'GnC_Cy Cuts at: 0 69 70 1033 1034 1061 1062 1187 1305 Size: 69 1 963 1 27 1 125 118 Fragments arranged by size: 963 125 118 69 27 1 1 1 HaeII r_GCGC'y Cuts at: 0 402 450 1305 Size: 402 48 855 Fragments arranged by size: 855 402 48 KasI G'GCGC_C Cuts at: 0 398 1305 Size: 398 907 MscI TGG'CCA Cuts at: 0 486 1305 Size: 486 819 MslI CAynn'nnrTG Cuts at: 0 739 1281 1305 Size: 739 542 24 Fragments arranged by size: 739 542 24 MspA1I CmG'CkG Cuts at: 0 200 234 564 946 1103 1305 Size: 200 34 330 382 157 202 Fragments arranged by size: 382 330 202 200 157 34 NarI GG'CG_CC Cuts at: 0 399 1305 Size: 399 906 NcoI C'CATG_G Cuts at: 0 1095 1305 Size: 1095 210 NspI r_CATG'y Cuts at: 0 744 1302 1305 Size: 744 558 3 Fragments arranged by size: 744 558 3 PasI CC'CwG_GG Cuts at: 0 184 936 1305 Size: 184 752 369 Fragments arranged by size: 752 369 184 PflMI CCAn_nnn'nTGG Cuts at: 0 104 1305 Size: 104 1201 PfoI T'CCnGG_A Cuts at: 0 329 1305 Size: 329 976 PspOMI G'GGCC_C Cuts at: 0 69 1033 1061 1186 1305 Size: 69 964 28 125 119 Fragments arranged by size: 964 125 119 69 28 PstI C_TGCA'G Cuts at: 0 1075 1305 Size: 1075 230 PvuII CAG'CTG Cuts at: 0 234 564 946 1305 Size: 234 330 382 359 Fragments arranged by size: 382 359 330 234 SacII CC_GC'GG Cuts at: 0 201 1305 Size: 201 1104 SapI GCTCTTCn'nnn_ Cuts at: 0 1288 1305 Size: 1288 17 SfcI C'TryA_G Cuts at: 0 222 1071 1305 Size: 222 849 234 Fragments arranged by size: 849 234 222 SfoI GGC'GCC Cuts at: 0 400 1305 Size: 400 905 StyI C'CwwG_G Cuts at: 0 57 428 986 1095 1305 Size: 57 371 558 109 210 Fragments arranged by size: 558 371 210 109 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 133 951 1305 Size: 133 818 354 Fragments arranged by size: 818 354 133 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 943 1174 1305 Size: 943 231 131 Fragments arranged by size: 943 231 131 Enzymes that do cut: AarI AfeI AleI AlfI ApaI BanI BanII BbeI BbsI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstEII BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoO109I HaeII KasI MscI MslI MspA1I NarI NcoI NspI PasI PflMI PfoI PspOMI PstI PvuII SacII SapI SfcI SfoI StyI TaqII XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BlpI BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI