Restriction map for
pFN21AE4547
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# NO CUT ENZYMES ( 96 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AvrII BamHI BbeI BciVI
BfaI BfrI BlnI BsaI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eam1105I Eco47III EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KasI
KpnI MaeI MfeI MluI MroI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 1003 GACGTC GACGT^C
AcsI 736 GAATTC [GA]^AATT[CT]
AcyI 1003 GACGTC G[GA]^CG[CT]C
AlwNI 1472 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1677 CCTTAGG CC^T[ACGT]AGG
ApoI 736 GAATTC [GA]^AATT[CT]
Asp700I 720 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1003 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 352 CCCGAG C^[CT]CG[GA]G
BalI 92 TGGCCA TGG^CCA
BbrPI 1409 CACGTG CAC^GTG
BbvCI 1585 GCTGAGG CCTCAGC(-5/-2)
BcgI 1806 GCAAATCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 434 TGATCA T^GATCA
BcnI 282 CCGGG CC^[GC]GG
BglI 1114 GCCTATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 359 AGATCT A^GATCT
BsaBI 1506 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1003 GACGTC G[GA]^CG[CT]C
BsmBI 391 GAGACG CGTCTC(1/5)
BsoBI 352 CCCGAG C^[CT]CG[GA]G
BsrI 272 ACTGG ACTGG(1/-1)
BssHII 810 GCGCGC G^CGCGC
BstAPI 1044 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1431 GGTGACC G^GT[ACGT]ACC
BstPI 1431 GGTGACC G^GT[ACGT]ACC
BstXI 122 CCAGCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1677 CCTTAGG CC^T[ACGT]AGG
DraIII 427 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 643 GACGCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1677 CCTTAGG CC^T[ACGT]AGG
EcoNI 864 CCTGTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1431 GGTGACC G^GT[ACGT]ACC
EcoRI 736 GAATTC G^AATTC
FbaI 434 TGATCA T^GATCA
HaeII 467 AGCGCC [GA]GCGC^[CT]
Hin1I 1003 GACGTC G[GA]^CG[CT]C
KspI 327 CCGCGG CCGC^GG
MamI 1506 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 92 TGGCCA TGG^CCA
MscI 92 TGGCCA TGG^CCA
NaeI 1529 GCCGGC GCC^GGC
NciI 282 CCGGG CC^[GC]GG
NcoI 368 CCATGG C^CATGG
NgoMIV 1529 GCCGGC G^CCGGC
PflFI 1003 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 723 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1409 CACGTG CAC^GTG
PmlI 1409 CACGTG CAC^GTG
PshAI 1720 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1054 CTGCAG CTGCA^G
SacI 16 GAGCTC GAGCT^C
SacII 327 CCGCGG CCGC^GG
SfcI 1054 CTGCAG C^T[GA][CT]AG
SmlI 1877 CTCAAG C^T[CT][GA]AG
Tsp509I 737 AATT ^AATT
Tth111I 1003 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 723 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1619 CCAAAAACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 720 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AfaI 405 GTAC GT^AC
1578 GTAC
AflIII 509 ACGTGT A^C[GA][CT]GT
887 ACACGT
AviII 785 TGCGCA TGC^GCA
794 TGCGCA
BanI 40 GGCACC G^G[CT][GA]CC
613 GGCACC
BbsI 1318 GTCTTC GAAGAC(2/6)
1642 GTCTTC
BlpI 375 GCTGAGC GC^T[ACGT]AGC
1533 GCTGAGC
Bpu1102I 375 GCTGAGC GC^T[ACGT]AGC
1533 GCTGAGC
BpuAI 1318 GTCTTC GAAGAC(2/6)
1642 GTCTTC
BsaAI 127 TACGTG [CT]AC^GT[GA]
1409 CACGTG
BsiEI 395 CGGCCG CG[GA][CT]^CG
1136 CGGCCG
BspHI 999 TCATGA T^CATGA
1563 TCATGA
BspMI 1367 ACCTGC ACCTGC(4/8)
1222 GCAGGT
BsrBI 148 GAGCGG CCGCTC(-3/-3)
765 GAGCGG
CelII 375 GCTGAGC GC^T[ACGT]AGC
1533 GCTGAGC
EagI 395 CGGCCG C^GGCCG
1136 CGGCCG
EclXI 395 CGGCCG C^GGCCG
1136 CGGCCG
Eco52I 395 CGGCCG C^GGCCG
1136 CGGCCG
FspI 785 TGCGCA TGC^GCA
794 TGCGCA
MaeIII 1432 GTGAC ^GT[ACGT]AC
1468 GTGAC
MslI 246 CACCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1323 CATCCAGGTG
PpuMI 758 AGGACCT [GA]G^G[AT]CC[CT]
1892 GGGACCC
PvuII 539 CAGCTG CAG^CTG
1759 CAGCTG
RcaI 999 TCATGA T^CATGA
1563 TCATGA
RsaI 405 GTAC GT^AC
1578 GTAC
TfiI 996 GATTC G^A[AT]TC
1689 GATTC
Tsp45I 1432 GTGAC ^GT[GC]AC
1468 GTGAC
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaII BanII BbvI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI TspRI TthHB8I
XhoII