(Circular) (Six-base) MAPSORT of: pFN21AE4547.seq Check: 6755 from: 1 to: 6634 pFN21AE4547 6634 bp With 182 enzymes: SgfI * March 28, 2012 14:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3218 3218 Size: 6634 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3012 4444 278 Size: 53 83 186 936 1476 1432 2468 Fragments arranged by size: 2468 1476 1432 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3977 1937 Size: 2040 4594 Acc65I G'GTAC_C Cuts at: 3955 3955 Size: 6634 AclI AA'CG_TT Cuts at: 1563 4761 5134 1563 Size: 3198 373 3063 Fragments arranged by size: 3198 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4697 6081 6349 1632 Size: 3065 1384 268 1917 Fragments arranged by size: 3065 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6437 AflIII A'CryG_T Cuts at: 1203 2514 2892 5533 6510 1203 Size: 1311 378 2641 977 1327 Fragments arranged by size: 2641 1327 1311 977 378 AgeI A'CCGG_T Cuts at: 5465 5465 Size: 6634 AhdI GACnn_n'nnGTC Cuts at: 5363 5363 Size: 6634 AleI CACnn'nnGTG Cuts at: 1166 2255 1166 Size: 1089 5545 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6634 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2333 2365 2333 Size: 32 6602 AlwNI CAG_nnn'CTG Cuts at: 3482 4377 5949 3482 Size: 895 1572 4167 Fragments arranged by size: 4167 1572 895 ApaLI G'TGCA_C Cuts at: 4691 5847 4691 Size: 1156 5478 ApoI r'AATT_y Cuts at: 1493 2741 3943 4169 4321 6430 1493 Size: 1248 1202 226 152 2109 1697 Fragments arranged by size: 2109 1697 1248 1202 226 152 AseI AT'TA_AT Cuts at: 160 1032 5188 160 Size: 872 4156 1606 Fragments arranged by size: 4156 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6634 AvaI C'yCGr_G Cuts at: 1870 1943 2357 3959 1870 Size: 73 414 1602 4545 Fragments arranged by size: 4545 1602 414 73 BamHI G'GATC_C Cuts at: 3964 4331 3964 Size: 367 6267 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 2618 3955 5410 618 Size: 325 217 627 258 573 1337 1455 1842 Fragments arranged by size: 1842 1455 1337 627 573 325 258 217 BanII G_rGCy'C Cuts at: 1391 2025 2511 2538 3029 3953 1391 Size: 634 486 27 491 924 4072 Fragments arranged by size: 4072 924 634 491 486 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3316 3640 928 Size: 971 1417 324 3922 Fragments arranged by size: 3922 1417 971 324 BbvCI CC'TCA_GC Cuts at: 3591 3591 Size: 6634 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3800 3834 4825 4859 1903 Size: 34 50 34 1779 34 991 34 3678 Fragments arranged by size: 3678 1779 991 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4526 5736 4526 Size: 1210 5424 BclI T'GATC_A Cuts at: 1548 2439 1548 Size: 891 5743 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3125 5245 Size: 107 122 71 631 605 337 1116 2120 Cuts at: 5245 136 Size: 1525 Fragments arranged by size: 2120 1525 1116 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2364 6629 2364 Size: 4265 2369 BlpI GC'TnA_GC Cuts at: 2381 3539 4051 2381 Size: 1158 512 4964 Fragments arranged by size: 4964 1158 512 Bme1580I G_kGCm'C Cuts at: 1790 2048 2451 2621 2788 2911 4695 5851 1790 Size: 258 403 170 167 123 1784 1156 2573 Fragments arranged by size: 2573 1784 1156 403 258 170 167 123 BmgBI CAC'GTC Cuts at: 1318 2895 1318 Size: 1577 5057 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4365 5323 442 Size: 416 53 83 399 24 2948 958 1753 Fragments arranged by size: 2948 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6634 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2383 2881 2383 Size: 498 6136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2700 2821 2876 2972 3239 3656 3773 3817 Size: 1461 121 55 96 267 417 117 44 Cuts at: 3817 3827 5294 1239 Size: 10 1467 2579 Fragments arranged by size: 2579 1467 1461 417 267 121 117 96 55 44 10 Bpu10I CC'TnA_GC Cuts at: 2768 3467 3591 2768 Size: 699 124 5811 Fragments arranged by size: 5811 699 124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3867 4759 5624 5922 3867 Size: 892 865 298 4579 Fragments arranged by size: 4579 892 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5297 882 Size: 617 3798 2219 Fragments arranged by size: 3798 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2134 3416 4425 493 Size: 711 930 1282 1009 2702 Fragments arranged by size: 2702 1282 1009 930 711 BsaBI GATnn'nnATC Cuts at: 3515 6628 3515 Size: 3113 3521 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3009 4441 4823 5458 Size: 53 83 186 936 1476 1432 382 635 Cuts at: 5458 275 Size: 1451 Fragments arranged by size: 1476 1451 1432 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5067 5465 5739 5886 6297 5067 Size: 398 274 147 411 5404 Fragments arranged by size: 5404 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2354 2384 3481 3511 1178 Size: 30 165 30 951 30 1097 30 4301 Fragments arranged by size: 4301 1097 951 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2375 2712 3563 3794 3938 1186 Size: 1189 337 851 231 144 3882 Fragments arranged by size: 3882 1189 851 337 231 144 BseYI C'CCAG_C Cuts at: 2126 3376 3902 5837 6333 2126 Size: 1250 526 1935 496 2427 Fragments arranged by size: 2427 1935 1250 526 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2279 3068 3155 3194 1163 Size: 573 543 789 87 39 4603 Fragments arranged by size: 4603 789 573 543 87 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2403 3144 4084 4845 5873 1540 Size: 275 186 402 741 940 761 1028 2301 Fragments arranged by size: 2301 1028 940 761 741 402 275 186 BsiHKAI G_wGCw'C Cuts at: 2025 2598 2930 3494 3759 4695 4780 5851 2025 Size: 573 332 564 265 936 85 1071 2808 Fragments arranged by size: 2808 1071 936 573 564 332 265 85 BsmI GAATG_Cn' Cuts at: 4145 4238 4145 Size: 93 6541 BsmBI CGTCTCn'nnnn_ Cuts at: 2390 2390 Size: 6634 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2048 2451 2511 2538 2598 2621 Size: 399 235 23 403 60 27 60 23 Cuts at: 2621 2788 2911 2930 3029 3494 3759 3953 4695 Size: 167 123 19 99 465 265 194 742 Cuts at: 4695 4780 5851 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 465 403 399 265 235 194 167 123 99 85 60 60 27 23 23 19 BspEI T'CCGG_A Cuts at: 6297 6297 Size: 6634 BspHI T'CATG_A Cuts at: 3004 3568 4523 6253 3004 Size: 564 955 1730 3385 Fragments arranged by size: 3385 1730 955 564 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3218 3381 3989 844 Size: 2374 163 608 3489 Fragments arranged by size: 3489 2374 608 163 BsrBI CCG'CTC Cuts at: 1394 1823 2155 2772 4521 1394 Size: 429 332 617 1749 3507 Fragments arranged by size: 3507 1749 617 429 332 BsrDI GCAATG_nn' Cuts at: 65 1248 5129 5303 65 Size: 1183 3881 174 1396 Fragments arranged by size: 3881 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2568 2715 2956 3534 3908 5278 5465 Size: 110 758 147 241 578 374 1370 187 Cuts at: 5465 1700 Size: 2869 Fragments arranged by size: 2869 1370 758 578 374 241 187 147 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6634 BssHII G'CGCG_C Cuts at: 1925 2815 1925 Size: 890 5744 BssSI C'ACGA_G Cuts at: 2056 2720 2904 4694 5706 2056 Size: 664 184 1790 1012 2984 Fragments arranged by size: 2984 1790 1012 664 184 BstAPI GCAn_nnn'nTGC Cuts at: 794 3055 794 Size: 2261 4373 BstBI TT'CG_AA Cuts at: 5438 5438 Size: 6634 BstEII G'GTnAC_C Cuts at: 1184 3436 1184 Size: 2252 4382 BstXI CCAn_nnnn'nTGG Cuts at: 2134 2134 Size: 6634 BstYI r'GATC_y Cuts at: 1972 2091 2364 3178 3964 4331 4716 4733 6185 Size: 119 273 814 786 367 385 17 1452 Cuts at: 6185 6271 6283 6629 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 814 786 385 367 346 273 119 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3683 3683 Size: 6634 BtgI C'CryG_G Cuts at: 513 1065 2015 2253 2332 2373 2538 513 Size: 552 950 238 79 41 165 4609 Fragments arranged by size: 4609 950 552 238 165 79 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2485 2536 487 Size: 683 56 1259 51 4585 Fragments arranged by size: 4585 1259 683 56 51 BtsI GCAGTG_nn' Cuts at: 2442 2673 4150 4944 4964 2442 Size: 231 1477 794 20 4112 Fragments arranged by size: 4112 1477 794 231 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6634 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6599 DraI TTT'AAA Cuts at: 3938 3938 Size: 6634 DraIII CAC_nnn'GTG Cuts at: 2437 2437 Size: 6634 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2654 5641 809 Size: 507 1338 2987 1802 Fragments arranged by size: 2987 1802 1338 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 2172 2400 Size: 54 1425 50 275 18 267 75 228 Cuts at: 2400 3141 4081 4970 6599 8 Size: 741 940 889 1629 43 Fragments arranged by size: 1629 1425 940 889 741 275 267 228 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2400 3141 4081 1537 Size: 275 588 741 940 4090 Fragments arranged by size: 4090 940 741 588 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2942 3696 3791 4564 1353 Size: 1589 754 95 773 3423 Fragments arranged by size: 3423 1589 773 754 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2764 5221 5595 5741 387 Size: 1064 138 1175 2457 374 146 1280 Fragments arranged by size: 2457 1280 1175 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2023 2023 Size: 6634 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2700 2821 2876 2972 3239 3656 3773 Size: 393 1068 121 55 96 267 417 117 Cuts at: 3773 3817 3827 4697 5294 6081 6349 1239 Size: 44 10 870 597 787 268 1524 Fragments arranged by size: 1524 1068 870 787 597 417 393 268 267 121 117 96 55 44 10 EcoNI CCTnn'n_nnAGG Cuts at: 2873 2873 Size: 6634 EcoO109I rG'GnC_Cy Cuts at: 2011 2764 2968 3848 3898 2011 Size: 753 204 880 50 4747 Fragments arranged by size: 4747 880 753 204 50 EcoRI G'AATT_C Cuts at: 2741 3943 4321 2741 Size: 1202 378 5054 Fragments arranged by size: 5054 1202 378 FspI TGC'GCA Cuts at: 2792 2801 5140 2792 Size: 9 2339 4286 Fragments arranged by size: 4286 2339 9 HaeII r_GCGC'y Cuts at: 1678 2476 5781 1678 Size: 798 3305 2531 Fragments arranged by size: 3305 2531 798 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2354 2386 5275 5307 5349 Size: 32 135 32 782 32 2889 32 42 Cuts at: 5349 5381 1373 Size: 32 2626 Fragments arranged by size: 2889 2626 782 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3978 4224 669 Size: 988 281 2040 246 3079 Fragments arranged by size: 3079 2040 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6634 HpaI GTT'AAC Cuts at: 4224 4224 Size: 6634 KpnI G_GTAC'C Cuts at: 3959 3959 Size: 6634 MfeI C'AATT_G Cuts at: 4233 4233 Size: 6634 MluI A'CGCG_T Cuts at: 6510 6510 Size: 6634 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2244 5748 5932 1305 Size: 939 3504 184 2007 Fragments arranged by size: 3504 2007 939 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2099 10 Size: 54 1425 343 267 4545 Fragments arranged by size: 4545 1425 343 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 3332 4592 4951 5110 518 Size: 648 1089 1077 1260 359 159 2042 Fragments arranged by size: 2042 1260 1089 1077 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2334 2546 3766 4050 4727 5875 6120 1606 Size: 728 212 1220 284 677 1148 245 2120 Fragments arranged by size: 2120 1220 1148 728 677 284 245 212 NaeI GCC'GGC Cuts at: 3536 3536 Size: 6634 NcoI C'CATG_G Cuts at: 513 1065 2373 513 Size: 552 1308 4774 Fragments arranged by size: 4774 1308 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6634 NgoMIV G'CCGG_C Cuts at: 3534 3534 Size: 6634 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6634 NotI GC'GGCC_GC Cuts at: 4081 4081 Size: 6634 NspI r_CATG'y Cuts at: 1132 1229 3992 5537 1132 Size: 97 2763 1545 2229 Fragments arranged by size: 2763 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3860 1348 Size: 2512 4122 PciI A'CATG_T Cuts at: 5533 5533 Size: 6634 PflMI CCAn_nnn'nTGG Cuts at: 1108 2734 1108 Size: 1626 5008 PmeI GTTT'AAAC Cuts at: 3938 3938 Size: 6634 PmlI CAC'GTG Cuts at: 3416 3416 Size: 6634 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4703 4735 6216 6248 4703 Size: 32 1481 32 5089 Fragments arranged by size: 5089 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2764 3898 2764 Size: 1134 5500 PshAI GACnn'nnGTC Cuts at: 1613 3729 1613 Size: 2116 4518 PsiI TTA'TAA Cuts at: 4204 4204 Size: 6634 PstI C_TGCA'G Cuts at: 830 3063 3986 830 Size: 2233 923 3478 Fragments arranged by size: 3478 2233 923 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6634 PvuII CAG'CTG Cuts at: 2546 3766 2546 Size: 1220 5414 SacI G_AGCT'C Cuts at: 2025 2025 Size: 6634 SacII CC_GC'GG Cuts at: 2335 2335 Size: 6634 SalI G'TCGA_C Cuts at: 1936 3976 1936 Size: 2040 4594 SanDI GG'GwC_CC Cuts at: 3898 3898 Size: 6634 SbfI CC_TGCA'GG Cuts at: 3986 3986 Size: 6634 ScaI AGT'ACT Cuts at: 4882 6594 4882 Size: 1712 4922 SfcI C'TryA_G Cuts at: 826 1045 3059 3982 5117 5798 5989 826 Size: 219 2014 923 1135 681 191 1471 Fragments arranged by size: 2014 1471 1135 923 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6634 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6634 SmaI CCC'GGG Cuts at: 1872 3961 1872 Size: 2089 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3882 4738 5639 5901 820 Size: 197 926 1939 856 901 262 1553 Fragments arranged by size: 1939 1553 926 901 856 262 197 SnaBI TAC'GTA Cuts at: 493 4425 493 Size: 3932 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6634 SphI G_CATG'C Cuts at: 1132 3992 1132 Size: 2860 3774 SspI AAT'ATT Cuts at: 5 52 4558 5 Size: 47 4506 2081 Fragments arranged by size: 4506 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6634 StyI C'CwwG_G Cuts at: 513 1065 2313 2373 2484 3042 3360 4073 513 Size: 552 1248 60 111 558 318 713 3074 Fragments arranged by size: 3074 1248 713 558 552 318 111 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2600 2858 4661 4678 4831 5016 5461 Size: 1255 1020 258 1803 17 153 185 445 Cuts at: 5461 325 Size: 1498 Fragments arranged by size: 1803 1498 1255 1020 445 258 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3582 4880 6592 Size: 274 80 33 51 1443 1605 1298 1712 Cuts at: 6592 96 Size: 138 Fragments arranged by size: 1712 1605 1443 1298 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2767 2799 3405 3437 6594 6626 2767 Size: 32 606 32 3157 32 2775 Fragments arranged by size: 3157 2775 606 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3011 1316 Size: 1695 4939 XbaI T'CTAG_A Cuts at: 3970 3970 Size: 6634 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3631 3631 Size: 6634 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6634 XmaI C'CCGG_G Cuts at: 1870 3959 1870 Size: 2089 4545 XmnI GAAnn'nnTTC Cuts at: 2729 4763 2729 Size: 2034 4600 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3010 4442 276 Size: 53 83 186 936 1476 1432 2468 Fragments arranged by size: 2468 1476 1432 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI