(Linear) (Six-base) MAPSORT of: KIEE4547.seq Check: 1775 from: 1 to: 1929 KIEE4547.orf With 182 enzymes: SgfI * March 22, 2012 18:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1213 1929 Size: 1213 716 AatII G_ACGT'C Cuts at: 0 1007 1929 Size: 1007 922 AflIII A'CryG_T Cuts at: 0 509 887 1929 Size: 509 378 1042 Fragments arranged by size: 1042 509 378 AleI CACnn'nnGTG Cuts at: 0 250 1929 Size: 250 1679 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 328 360 1929 Size: 328 32 1569 Fragments arranged by size: 1569 328 32 AlwNI CAG_nnn'CTG Cuts at: 0 1477 1929 Size: 1477 452 ApoI r'AATT_y Cuts at: 0 736 1929 Size: 736 1193 AvaI C'yCGr_G Cuts at: 0 352 1929 Size: 352 1577 BanI G'GyrC_C Cuts at: 0 40 613 1929 Size: 40 573 1316 Fragments arranged by size: 1316 573 40 BanII G_rGCy'C Cuts at: 0 20 506 533 1024 1929 Size: 20 486 27 491 905 Fragments arranged by size: 905 491 486 27 20 BbsI GAAGACnn'nnnn_ Cuts at: 0 1311 1635 1929 Size: 1311 324 294 Fragments arranged by size: 1311 324 294 BbvCI CC'TCA_GC Cuts at: 0 1586 1929 Size: 1586 343 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1795 1829 1929 Size: 1795 34 100 Fragments arranged by size: 1795 100 34 BclI T'GATC_A Cuts at: 0 434 1929 Size: 434 1495 BglI GCCn_nnn'nGGC Cuts at: 0 1120 1929 Size: 1120 809 BglII A'GATC_T Cuts at: 0 359 1929 Size: 359 1570 BlpI GC'TnA_GC Cuts at: 0 376 1534 1929 Size: 376 1158 395 Fragments arranged by size: 1158 395 376 Bme1580I G_kGCm'C Cuts at: 0 43 446 616 783 906 1929 Size: 43 403 170 167 123 1023 Fragments arranged by size: 1023 403 170 167 123 43 BmgBI CAC'GTC Cuts at: 0 890 1929 Size: 890 1039 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 378 876 1929 Size: 378 498 1053 Fragments arranged by size: 1053 498 378 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 695 816 871 967 1234 1651 1768 1812 Size: 695 121 55 96 267 417 117 44 Cuts at: 1812 1822 1929 Size: 10 107 Fragments arranged by size: 695 417 267 121 117 107 96 55 44 10 Bpu10I CC'TnA_GC Cuts at: 0 763 1462 1586 1929 Size: 763 699 124 343 Fragments arranged by size: 763 699 343 124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1862 1929 Size: 1862 67 BsaAI yAC'GTr Cuts at: 0 129 1411 1929 Size: 129 1282 518 Fragments arranged by size: 1282 518 129 BsaBI GATnn'nnATC Cuts at: 0 1510 1929 Size: 1510 419 BsaHI Gr'CG_yC Cuts at: 0 1004 1929 Size: 1004 925 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 349 379 1476 1506 1929 Size: 349 30 1097 30 423 Fragments arranged by size: 1097 423 349 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 370 707 1558 1789 1929 Size: 370 337 851 231 140 Fragments arranged by size: 851 370 337 231 140 BseYI C'CCAG_C Cuts at: 0 121 1371 1897 1929 Size: 121 1250 526 32 Fragments arranged by size: 1250 526 121 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 274 1063 1150 1189 1929 Size: 274 789 87 39 740 Fragments arranged by size: 789 740 274 87 39 BsiEI CG_ry'CG Cuts at: 0 398 1139 1929 Size: 398 741 790 Fragments arranged by size: 790 741 398 BsiHKAI G_wGCw'C Cuts at: 0 20 593 925 1489 1754 1929 Size: 20 573 332 564 265 175 Fragments arranged by size: 573 564 332 265 175 20 BsmBI CGTCTCn'nnnn_ Cuts at: 0 385 1929 Size: 385 1544 Bsp1286I G_dGCh'C Cuts at: 0 20 43 446 506 533 593 616 783 Size: 20 23 403 60 27 60 23 167 Cuts at: 783 906 925 1024 1489 1754 1929 Size: 123 19 99 465 265 175 Fragments arranged by size: 465 403 265 175 167 123 99 60 60 27 23 23 20 19 BspHI T'CATG_A Cuts at: 0 999 1563 1929 Size: 999 564 366 Fragments arranged by size: 999 564 366 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1213 1376 1929 Size: 1213 163 553 Fragments arranged by size: 1213 553 163 BsrBI CCG'CTC Cuts at: 0 150 767 1929 Size: 150 617 1162 Fragments arranged by size: 1162 617 150 BsrFI r'CCGG_y Cuts at: 0 563 710 951 1529 1903 1929 Size: 563 147 241 578 374 26 Fragments arranged by size: 578 563 374 241 147 26 BssHII G'CGCG_C Cuts at: 0 810 1929 Size: 810 1119 BssSI C'ACGA_G Cuts at: 0 51 715 899 1929 Size: 51 664 184 1030 Fragments arranged by size: 1030 664 184 51 BstAPI GCAn_nnn'nTGC Cuts at: 0 1050 1929 Size: 1050 879 BstEII G'GTnAC_C Cuts at: 0 1431 1929 Size: 1431 498 BstXI CCAn_nnnn'nTGG Cuts at: 0 129 1929 Size: 129 1800 BstYI r'GATC_y Cuts at: 0 86 359 1173 1929 Size: 86 273 814 756 Fragments arranged by size: 814 756 273 86 Bsu36I CC'TnA_GG Cuts at: 0 1678 1929 Size: 1678 251 BtgI C'CryG_G Cuts at: 0 10 248 327 368 533 1929 Size: 10 238 79 41 165 1396 Fragments arranged by size: 1396 238 165 79 41 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 480 531 1929 Size: 480 51 1398 Fragments arranged by size: 1398 480 51 BtsI GCAGTG_nn' Cuts at: 0 437 668 1929 Size: 437 231 1261 Fragments arranged by size: 1261 437 231 DraIII CAC_nnn'GTG Cuts at: 0 432 1929 Size: 432 1497 DrdI GACnn_nn'nnGTC Cuts at: 0 649 1929 Size: 649 1280 EaeI y'GGCC_r Cuts at: 0 92 167 395 1136 1929 Size: 92 75 228 741 793 Fragments arranged by size: 793 741 228 92 75 EagI C'GGCC_G Cuts at: 0 395 1136 1929 Size: 395 741 793 Fragments arranged by size: 793 741 395 EarI CTCTTCn'nnn_ Cuts at: 0 937 1691 1786 1929 Size: 937 754 95 143 Fragments arranged by size: 937 754 143 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 759 1929 Size: 759 1170 EcoICRI GAG'CTC Cuts at: 0 18 1929 Size: 18 1911 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 695 816 871 967 1234 1651 1768 1812 Size: 695 121 55 96 267 417 117 44 Cuts at: 1812 1822 1929 Size: 10 107 Fragments arranged by size: 695 417 267 121 117 107 96 55 44 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 868 1929 Size: 868 1061 EcoO109I rG'GnC_Cy Cuts at: 0 6 759 963 1843 1893 1929 Size: 6 753 204 880 50 36 Fragments arranged by size: 880 753 204 50 36 6 EcoRI G'AATT_C Cuts at: 0 736 1929 Size: 736 1193 FspI TGC'GCA Cuts at: 0 787 796 1929 Size: 787 9 1133 Fragments arranged by size: 1133 787 9 HaeII r_GCGC'y Cuts at: 0 471 1929 Size: 471 1458 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 349 381 1929 Size: 349 32 1548 Fragments arranged by size: 1548 349 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 239 1929 Size: 239 1690 MscI TGG'CCA Cuts at: 0 94 1929 Size: 94 1835 MslI CAynn'nnrTG Cuts at: 0 250 1327 1929 Size: 250 1077 602 Fragments arranged by size: 1077 602 250 MspA1I CmG'CkG Cuts at: 0 329 541 1761 1929 Size: 329 212 1220 168 Fragments arranged by size: 1220 329 212 168 NaeI GCC'GGC Cuts at: 0 1531 1929 Size: 1531 398 NcoI C'CATG_G Cuts at: 0 368 1929 Size: 368 1561 NgoMIV G'CCGG_C Cuts at: 0 1529 1929 Size: 1529 400 PasI CC'CwG_GG Cuts at: 0 1855 1929 Size: 1855 74 PflMI CCAn_nnn'nTGG Cuts at: 0 729 1929 Size: 729 1200 PmlI CAC'GTG Cuts at: 0 1411 1929 Size: 1411 518 PpuMI rG'GwC_Cy Cuts at: 0 759 1893 1929 Size: 759 1134 36 Fragments arranged by size: 1134 759 36 PshAI GACnn'nnGTC Cuts at: 0 1724 1929 Size: 1724 205 PstI C_TGCA'G Cuts at: 0 1058 1929 Size: 1058 871 PvuII CAG'CTG Cuts at: 0 541 1761 1929 Size: 541 1220 168 Fragments arranged by size: 1220 541 168 SacI G_AGCT'C Cuts at: 0 20 1929 Size: 20 1909 SacII CC_GC'GG Cuts at: 0 330 1929 Size: 330 1599 SanDI GG'GwC_CC Cuts at: 0 1893 1929 Size: 1893 36 SfcI C'TryA_G Cuts at: 0 1054 1929 Size: 1054 875 SmlI C'TyrA_G Cuts at: 0 1877 1929 Size: 1877 52 StyI C'CwwG_G Cuts at: 0 308 368 479 1037 1355 1929 Size: 308 60 111 558 318 574 Fragments arranged by size: 574 558 318 308 111 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 595 853 1929 Size: 595 258 1076 Fragments arranged by size: 1076 595 258 TatI w'GTAC_w Cuts at: 0 1577 1929 Size: 1577 352 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 762 794 1400 1432 1929 Size: 762 32 606 32 497 Fragments arranged by size: 762 606 497 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1006 1929 Size: 1006 923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1626 1929 Size: 1626 303 XmnI GAAnn'nnTTC Cuts at: 0 724 1929 Size: 724 1205 ZraI GAC'GTC Cuts at: 0 1005 1929 Size: 1005 924 Enzymes that do cut: AarI AatII AflIII AleI AloI AlwNI ApoI AvaI BanI BanII BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PmlI PpuMI PshAI PstI PvuII SacI SacII SanDI SfcI SmlI StyI TaqII TatI TstI Tth111I XcmI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BfrBI BmrI BmtI BsaI BsaWI BsiWI BsmI BspEI BsrDI BsrGI BstBI BstZ17I ClaI CspCI DraI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI XmaI