(Circular) (Six-base) MAPSORT of: pFN21AE1468.seq Check: 628 from: 1 to: 7546 pFN21AE1468 7546 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4629 4629 Size: 7546 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3552 5356 278 Size: 53 83 186 936 2016 1804 2468 Fragments arranged by size: 2468 2016 1804 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2121 2874 2925 4889 1937 Size: 184 753 51 1964 4594 Fragments arranged by size: 4594 1964 753 184 51 Acc65I G'GTAC_C Cuts at: 4867 4867 Size: 7546 AclI AA'CG_TT Cuts at: 1563 5673 6046 1563 Size: 4110 373 3063 Fragments arranged by size: 4110 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2624 4007 4684 5609 6993 7261 1632 Size: 992 1383 677 925 1384 268 1917 Fragments arranged by size: 1917 1384 1383 992 925 677 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7349 AflIII A'CryG_T Cuts at: 1203 2718 6445 7422 1203 Size: 1515 3727 977 1327 Fragments arranged by size: 3727 1515 1327 977 AgeI A'CCGG_T Cuts at: 6377 6377 Size: 7546 AhdI GACnn_n'nnGTC Cuts at: 6275 6275 Size: 7546 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7546 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4784 1184 Size: 3600 3946 AlwNI CAG_nnn'CTG Cuts at: 3502 3720 3753 4259 4312 4321 5289 6861 3502 Size: 218 33 506 53 9 968 1572 4187 Fragments arranged by size: 4187 1572 968 506 218 53 33 9 ApaI G_GGCC'C Cuts at: 3481 4096 4104 3481 Size: 615 8 6923 Fragments arranged by size: 6923 615 8 ApaLI G'TGCA_C Cuts at: 3245 4774 5603 6759 3245 Size: 1529 829 1156 4032 Fragments arranged by size: 4032 1529 1156 829 ApoI r'AATT_y Cuts at: 1493 4383 4855 5081 5233 7342 1493 Size: 2890 472 226 152 2109 1697 Fragments arranged by size: 2890 2109 1697 472 226 152 AseI AT'TA_AT Cuts at: 160 1032 6100 160 Size: 872 5068 1606 Fragments arranged by size: 5068 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7546 AvaI C'yCGr_G Cuts at: 1870 1943 3105 4545 4756 4871 1870 Size: 73 1162 1440 211 115 4545 Fragments arranged by size: 4545 1440 1162 211 115 73 BamHI G'GATC_C Cuts at: 4876 5243 4876 Size: 367 7179 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 2561 2981 4747 4867 Size: 325 217 627 246 528 420 1766 120 Cuts at: 4867 6322 618 Size: 1455 1842 Fragments arranged by size: 1842 1766 1455 627 528 420 325 246 217 120 BanII G_rGCy'C Cuts at: 1391 2117 2150 2456 2964 3044 3098 3114 3481 Size: 726 33 306 508 80 54 16 367 Cuts at: 3481 3918 3995 4096 4104 4135 4167 4481 4865 Size: 437 77 101 8 31 32 314 384 Cuts at: 4865 1391 Size: 4072 Fragments arranged by size: 4072 726 508 437 384 367 314 306 101 80 77 54 33 32 31 16 8 BbeI G_GCGC'C Cuts at: 2565 2565 Size: 7546 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2093 3617 928 Size: 971 194 1524 4857 Fragments arranged by size: 4857 1524 971 194 BbvCI CC'TCA_GC Cuts at: 3905 4113 4397 3905 Size: 208 284 7054 Fragments arranged by size: 7054 284 208 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2290 2324 4712 4746 5737 5771 1903 Size: 34 353 34 2388 34 991 34 3678 Fragments arranged by size: 3678 2388 991 353 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5438 6648 5438 Size: 1210 6336 BclI T'GATC_A Cuts at: 1548 2347 2691 1548 Size: 799 344 6403 Fragments arranged by size: 6403 799 344 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6157 136 Size: 107 122 71 631 605 4485 1525 Fragments arranged by size: 4485 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7541 7541 Size: 7546 BlpI GC'TnA_GC Cuts at: 2044 4963 2044 Size: 2919 4627 Bme1580I G_kGCm'C Cuts at: 1790 2038 2201 2986 3249 3481 3967 4096 4104 Size: 248 163 785 263 232 486 129 8 Cuts at: 4104 4778 5607 6763 1790 Size: 674 829 1156 2573 Fragments arranged by size: 2573 1156 829 785 674 486 263 248 232 163 129 8 BmgBI CAC'GTC Cuts at: 1318 2185 1318 Size: 867 6679 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2108 2528 3208 Size: 416 53 83 399 24 691 420 680 Cuts at: 3208 5277 6235 442 Size: 2069 958 1753 Fragments arranged by size: 2069 1753 958 691 680 420 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7546 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3830 Size: 7546 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2804 2864 2978 3082 3524 3763 4127 4268 Size: 1565 60 114 104 442 239 364 141 Cuts at: 4268 4388 4507 4516 4825 4831 4853 6206 1239 Size: 120 119 9 309 6 22 1353 2579 Fragments arranged by size: 2579 1565 1353 442 364 309 239 141 120 119 114 104 60 22 9 6 Bpu10I CC'TnA_GC Cuts at: 2511 2906 3043 3905 4113 4137 4397 4475 4480 Size: 395 137 862 208 24 260 78 5 Cuts at: 4480 2511 Size: 5577 Fragments arranged by size: 5577 862 395 260 208 137 78 24 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2150 3146 3806 5671 6536 6834 2150 Size: 996 660 1865 865 298 2862 Fragments arranged by size: 2862 1865 996 865 660 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3677 6209 882 Size: 617 2178 2532 2219 Fragments arranged by size: 2532 2219 2178 617 BsaAI yAC'GTr Cuts at: 493 1204 2719 5337 493 Size: 711 1515 2618 2702 Fragments arranged by size: 2702 2618 1515 711 BsaBI GATnn'nnATC Cuts at: 7540 7540 Size: 7546 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2090 2562 3549 3726 Size: 53 83 186 936 557 472 987 177 Cuts at: 3726 5353 5735 6370 275 Size: 1627 382 635 1451 Fragments arranged by size: 1627 1451 987 936 635 557 472 382 186 177 83 53 BsaWI w'CCGG_w Cuts at: 3697 5979 6377 6651 6798 7209 3697 Size: 2282 398 274 147 411 4034 Fragments arranged by size: 4034 2282 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2534 2564 3939 3969 4397 Size: 30 165 30 1131 30 1375 30 428 Cuts at: 4397 4427 1178 Size: 30 4297 Fragments arranged by size: 4297 1375 1131 428 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3335 4130 4140 4176 4223 4385 1186 Size: 2149 795 10 36 47 162 4347 Fragments arranged by size: 4347 2149 795 162 47 36 10 BseYI C'CCAG_C Cuts at: 2312 2963 3113 3709 3943 4068 4271 4353 4687 Size: 651 150 596 234 125 203 82 334 Cuts at: 4687 6749 7245 2312 Size: 2062 496 2613 Fragments arranged by size: 2613 2062 651 596 496 334 234 203 150 125 82 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2509 2558 2714 3357 3749 4607 1163 Size: 573 773 49 156 643 392 858 4102 Fragments arranged by size: 4102 858 773 643 573 392 156 49 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2660 4996 5757 6785 1540 Size: 275 186 659 2336 761 1028 2301 Fragments arranged by size: 2336 2301 1028 761 659 275 186 BsiHKAI G_wGCw'C Cuts at: 2117 2456 3044 3249 3570 4481 4778 5607 5692 Size: 339 588 205 321 911 297 829 85 Cuts at: 5692 6763 2117 Size: 1071 2900 Fragments arranged by size: 2900 1071 911 829 588 339 321 297 205 85 BsmI GAATG_Cn' Cuts at: 5057 5150 5057 Size: 93 7453 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 2117 2150 2201 2456 2964 2986 Size: 399 248 79 33 51 255 508 22 Cuts at: 2986 3044 3098 3114 3249 3481 3570 3918 3967 Size: 58 54 16 135 232 89 348 49 Cuts at: 3967 3995 4096 4104 4135 4167 4481 4778 4865 Size: 28 101 8 31 32 314 297 87 Cuts at: 4865 5607 5692 6763 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 508 399 348 314 297 255 248 232 135 101 89 87 85 79 58 54 51 49 33 32 31 28 22 16 8 BspEI T'CCGG_A Cuts at: 3697 7209 3697 Size: 3512 4034 BspHI T'CATG_A Cuts at: 5435 7165 5435 Size: 1730 5816 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4629 4776 4901 844 Size: 3785 147 125 3489 Fragments arranged by size: 3785 3489 147 125 BsrBI CCG'CTC Cuts at: 1394 1823 3814 4285 5433 1394 Size: 429 1991 471 1148 3507 Fragments arranged by size: 3507 1991 1148 471 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2711 6041 6215 65 Size: 1183 1463 3330 174 1396 Fragments arranged by size: 3330 1463 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2659 6190 6377 1700 Size: 110 849 3531 187 2869 Fragments arranged by size: 3531 2869 849 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7546 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7546 BssSI C'ACGA_G Cuts at: 2976 4390 5606 6618 2976 Size: 1414 1216 1012 3904 Fragments arranged by size: 3904 1414 1216 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3025 3720 4312 794 Size: 2231 695 592 4028 Fragments arranged by size: 4028 2231 695 592 BstBI TT'CG_AA Cuts at: 6350 6350 Size: 7546 BstEII G'GTnAC_C Cuts at: 1184 4708 1184 Size: 3524 4022 BstXI CCAn_nnnn'nTGG Cuts at: 2365 2365 Size: 7546 BstYI r'GATC_y Cuts at: 1972 2172 2352 3101 3855 4683 4876 5243 5628 Size: 200 180 749 754 828 193 367 385 Cuts at: 5628 5645 7097 7183 7195 7541 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 828 754 749 385 367 346 200 193 180 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2136 513 Size: 552 939 132 5923 Fragments arranged by size: 5923 939 552 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2230 2293 3594 4645 487 Size: 683 56 1004 63 1301 1051 3388 Fragments arranged by size: 3388 1301 1051 1004 683 63 56 BtsI GCAGTG_nn' Cuts at: 2016 2055 4778 5062 5856 5876 2016 Size: 39 2723 284 794 20 3686 Fragments arranged by size: 3686 2723 794 284 39 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7546 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7511 DraI TTT'AAA Cuts at: 4850 4850 Size: 7546 DraIII CAC_nnn'GTG Cuts at: 3928 3928 Size: 7546 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6553 809 Size: 507 5237 1802 Fragments arranged by size: 5237 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2487 2657 3072 Size: 54 1425 50 275 18 657 170 415 Cuts at: 3072 3610 4106 4825 4993 5882 7511 8 Size: 538 496 719 168 889 1629 43 Fragments arranged by size: 1629 1425 889 719 657 538 496 415 275 170 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2657 4993 1537 Size: 275 845 2336 4090 Fragments arranged by size: 4090 2336 845 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2138 2444 3983 5476 1353 Size: 785 306 1539 1493 3423 Fragments arranged by size: 3423 1539 1493 785 306 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3206 3831 6133 6507 6653 387 Size: 1064 138 1617 625 2302 374 146 1280 Fragments arranged by size: 2302 1617 1280 1064 625 374 146 138 EcoICRI GAG'CTC Cuts at: 2115 2454 3042 4479 2115 Size: 339 588 1437 5182 Fragments arranged by size: 5182 1437 588 339 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2624 2804 2864 2978 3082 3524 3763 Size: 393 992 180 60 114 104 442 239 Cuts at: 3763 4007 4127 4268 4388 4507 4516 4684 4825 Size: 244 120 141 120 119 9 168 141 Cuts at: 4825 4831 4853 5609 6206 6993 7261 1239 Size: 6 22 756 597 787 268 1524 Fragments arranged by size: 1524 992 787 756 597 442 393 268 244 239 180 168 141 141 120 120 119 114 104 60 22 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 3494 3494 Size: 7546 EcoO109I rG'GnC_Cy Cuts at: 2505 2650 2750 3389 3478 3499 4092 4093 4100 Size: 145 100 639 89 21 593 1 7 Cuts at: 4100 4101 2505 Size: 1 5950 Fragments arranged by size: 5950 639 593 145 100 89 21 7 1 1 EcoRI G'AATT_C Cuts at: 4855 5233 4855 Size: 378 7168 FspI TGC'GCA Cuts at: 6052 6052 Size: 7546 HaeII r_GCGC'y Cuts at: 1678 2565 6693 1678 Size: 887 4128 2531 Fragments arranged by size: 4128 2531 887 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2332 2364 2534 2566 2664 Size: 32 135 32 760 32 170 32 98 Cuts at: 2664 2696 3177 3209 4397 4429 6187 6219 6261 Size: 32 481 32 1188 32 1758 32 42 Cuts at: 6261 6293 1373 Size: 32 2626 Fragments arranged by size: 2626 1758 1188 760 481 170 135 98 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3400 4890 5136 669 Size: 988 281 1462 1490 246 3079 Fragments arranged by size: 3079 1490 1462 988 281 246 HindIII A'AGCT_T Cuts at: 748 2126 748 Size: 1378 6168 HpaI GTT'AAC Cuts at: 5136 5136 Size: 7546 KasI G'GCGC_C Cuts at: 2561 2561 Size: 7546 KpnI G_GTAC'C Cuts at: 4871 4871 Size: 7546 MfeI C'AATT_G Cuts at: 5145 5145 Size: 7546 MluI A'CGCG_T Cuts at: 7422 7422 Size: 7546 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2384 4401 6660 6844 1305 Size: 1079 2017 2259 184 2007 Fragments arranged by size: 2259 2017 2007 1079 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2489 3074 3612 4827 10 Size: 54 1425 343 657 585 538 1215 2729 Fragments arranged by size: 2729 1425 1215 657 585 538 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2534 3407 4745 5504 5863 6022 Size: 648 843 525 873 1338 759 359 159 Cuts at: 6022 518 Size: 2042 Fragments arranged by size: 2042 1338 873 843 759 648 525 359 159 MspA1I CmG'CkG Cuts at: 1606 2231 2967 3442 3750 3938 4112 4321 4527 Size: 625 736 475 308 188 174 209 206 Cuts at: 4527 4691 4831 4962 5639 6787 7032 1606 Size: 164 140 131 677 1148 245 2120 Fragments arranged by size: 2120 1148 736 677 625 475 308 245 209 206 188 174 164 140 131 NaeI GCC'GGC Cuts at: 2661 2661 Size: 7546 NarI GG'CG_CC Cuts at: 2562 2562 Size: 7546 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6055 Fragments arranged by size: 6055 939 552 NdeI CA'TA_TG Cuts at: 387 2569 387 Size: 2182 5364 NgoMIV G'CCGG_C Cuts at: 2659 2659 Size: 7546 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7546 NotI GC'GGCC_GC Cuts at: 4993 4993 Size: 7546 NspI r_CATG'y Cuts at: 1132 1229 3023 3336 3772 4904 6449 1132 Size: 97 1794 313 436 1132 1545 2229 Fragments arranged by size: 2229 1794 1545 1132 436 313 97 PasI CC'CwG_GG Cuts at: 1348 3433 4097 1348 Size: 2085 664 4797 Fragments arranged by size: 4797 2085 664 PciI A'CATG_T Cuts at: 6445 6445 Size: 7546 PflMI CCAn_nnn'nTGG Cuts at: 1108 2618 3081 3502 1108 Size: 1510 463 421 5152 Fragments arranged by size: 5152 1510 463 421 PfoI T'CCnGG_A Cuts at: 4299 4493 4629 4299 Size: 194 136 7216 Fragments arranged by size: 7216 194 136 PmeI GTTT'AAAC Cuts at: 4850 4850 Size: 7546 PmlI CAC'GTG Cuts at: 2719 2719 Size: 7546 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5615 5647 7128 7160 5615 Size: 32 1481 32 6001 Fragments arranged by size: 6001 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2505 2505 Size: 7546 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7546 PsiI TTA'TAA Cuts at: 5116 5116 Size: 7546 PspOMI G'GGCC_C Cuts at: 3477 4092 4100 3477 Size: 615 8 6923 Fragments arranged by size: 6923 615 8 PstI C_TGCA'G Cuts at: 830 3937 4317 4696 4773 4898 830 Size: 3107 380 379 77 125 3478 Fragments arranged by size: 3478 3107 380 379 125 77 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7546 PvuII CAG'CTG Cuts at: 2231 2967 3442 3750 4112 4321 4527 4691 4831 Size: 736 475 308 362 209 206 164 140 Cuts at: 4831 2231 Size: 4946 Fragments arranged by size: 4946 736 475 362 308 209 206 164 140 SacI G_AGCT'C Cuts at: 2117 2456 3044 4481 2117 Size: 339 588 1437 5182 Fragments arranged by size: 5182 1437 588 339 SalI G'TCGA_C Cuts at: 1936 4888 1936 Size: 2952 4594 SapI GCTCTTCn'nnn_ Cuts at: 2138 2444 3983 2138 Size: 306 1539 5701 Fragments arranged by size: 5701 1539 306 SbfI CC_TGCA'GG Cuts at: 4898 4898 Size: 7546 ScaI AGT'ACT Cuts at: 5794 7506 5794 Size: 1712 5834 SfcI C'TryA_G Cuts at: 826 1045 2376 2782 3933 3968 4313 4692 4769 Size: 219 1331 406 1151 35 345 379 77 Cuts at: 4769 4894 6029 6710 6901 826 Size: 125 1135 681 191 1471 Fragments arranged by size: 1471 1331 1151 1135 681 406 379 345 219 191 125 77 35 SfoI GGC'GCC Cuts at: 2563 2563 Size: 7546 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7546 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7546 SmaI CCC'GGG Cuts at: 1872 3107 4547 4873 1872 Size: 1235 1440 326 4545 Fragments arranged by size: 4545 1440 1235 326 SmlI C'TyrA_G Cuts at: 820 1017 1943 2129 3161 3821 5650 6551 6813 Size: 197 926 186 1032 660 1829 901 262 Cuts at: 6813 820 Size: 1553 Fragments arranged by size: 1829 1553 1032 926 901 660 262 197 186 SnaBI TAC'GTA Cuts at: 493 5337 493 Size: 4844 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7546 SphI G_CATG'C Cuts at: 1132 3023 4904 1132 Size: 1891 1881 3774 Fragments arranged by size: 3774 1891 1881 SspI AAT'ATT Cuts at: 5 52 5470 5 Size: 47 5418 2081 Fragments arranged by size: 5418 2081 47 StuI AGG'CCT Cuts at: 1517 2616 4451 1517 Size: 1099 1835 4612 Fragments arranged by size: 4612 1835 1099 StyI C'CwwG_G Cuts at: 513 1065 2004 2079 2193 2205 2484 2617 3392 Size: 552 939 75 114 12 279 133 775 Cuts at: 3392 3729 3995 4088 4985 513 Size: 337 266 93 897 3074 Fragments arranged by size: 3074 939 897 775 552 337 279 266 133 114 93 75 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3367 5573 5590 5743 5928 6373 325 Size: 1255 1787 2206 17 153 185 445 1498 Fragments arranged by size: 2206 1787 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2325 3483 5792 Size: 274 80 33 51 1443 348 1158 2309 Cuts at: 5792 7504 96 Size: 1712 138 Fragments arranged by size: 2309 1712 1443 1158 348 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3939 3971 7506 7538 3939 Size: 32 3535 32 3947 Fragments arranged by size: 3947 3535 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7546 XbaI T'CTAG_A Cuts at: 4882 4882 Size: 7546 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 4531 2011 Size: 2520 5026 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7546 XmaI C'CCGG_G Cuts at: 1870 3105 4545 4871 1870 Size: 1235 1440 326 4545 Fragments arranged by size: 4545 1440 1235 326 XmnI GAAnn'nnTTC Cuts at: 3309 5675 3309 Size: 2366 5180 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3550 5354 276 Size: 53 83 186 936 2016 1804 2468 Fragments arranged by size: 2468 2016 1804 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI