(Linear) (Six-base) MAPSORT of: KIEE1468.seq Check: 8533 from: 1 to: 2841 KIEE1468.orf With 182 enzymes: SgfI * July 3, 2012 16:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2624 2841 Size: 2624 217 AatII G_ACGT'C Cuts at: 0 1547 2841 Size: 1547 1294 AccI GT'mk_AC Cuts at: 0 116 869 920 2841 Size: 116 753 51 1921 Fragments arranged by size: 1921 753 116 51 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 619 2002 2679 2841 Size: 619 1383 677 162 Fragments arranged by size: 1383 677 619 162 AflIII A'CryG_T Cuts at: 0 713 2841 Size: 713 2128 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2779 2841 Size: 2779 62 AlwNI CAG_nnn'CTG Cuts at: 0 1497 1715 1748 2254 2307 2316 2841 Size: 1497 218 33 506 53 9 525 Fragments arranged by size: 1497 525 506 218 53 33 9 ApaI G_GGCC'C Cuts at: 0 1476 2091 2099 2841 Size: 1476 615 8 742 Fragments arranged by size: 1476 742 615 8 ApaLI G'TGCA_C Cuts at: 0 1240 2769 2841 Size: 1240 1529 72 Fragments arranged by size: 1529 1240 72 ApoI r'AATT_y Cuts at: 0 2378 2841 Size: 2378 463 AvaI C'yCGr_G Cuts at: 0 1100 2540 2751 2841 Size: 1100 1440 211 90 Fragments arranged by size: 1440 1100 211 90 BanI G'GyrC_C Cuts at: 0 28 556 976 2742 2841 Size: 28 528 420 1766 99 Fragments arranged by size: 1766 528 420 99 28 BanII G_rGCy'C Cuts at: 0 112 145 451 959 1039 1093 1109 1476 Size: 112 33 306 508 80 54 16 367 Cuts at: 1476 1913 1990 2091 2099 2130 2162 2476 2841 Size: 437 77 101 8 31 32 314 365 Fragments arranged by size: 508 437 367 365 314 306 112 101 80 77 54 33 32 31 16 8 BbeI G_GCGC'C Cuts at: 0 560 2841 Size: 560 2281 BbsI GAAGACnn'nnnn_ Cuts at: 0 88 1612 2841 Size: 88 1524 1229 Fragments arranged by size: 1524 1229 88 BbvCI CC'TCA_GC Cuts at: 0 1900 2108 2392 2841 Size: 1900 208 284 449 Fragments arranged by size: 1900 449 284 208 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 285 319 2707 2741 2841 Size: 285 34 2388 34 100 Fragments arranged by size: 2388 285 100 34 34 BclI T'GATC_A Cuts at: 0 342 686 2841 Size: 342 344 2155 Fragments arranged by size: 2155 344 342 BlpI GC'TnA_GC Cuts at: 0 39 2841 Size: 39 2802 Bme1580I G_kGCm'C Cuts at: 0 33 196 981 1244 1476 1962 2091 2099 Size: 33 163 785 263 232 486 129 8 Cuts at: 2099 2773 2841 Size: 674 68 Fragments arranged by size: 785 674 486 263 232 163 129 68 33 8 BmgBI CAC'GTC Cuts at: 0 180 2841 Size: 180 2661 BmrI ACTGGGnnnn_n' Cuts at: 0 103 523 1203 2841 Size: 103 420 680 1638 Fragments arranged by size: 1638 680 420 103 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1825 2841 Size: 1825 1016 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 799 859 973 1077 1519 1758 2122 2263 Size: 799 60 114 104 442 239 364 141 Cuts at: 2263 2383 2502 2511 2820 2826 2841 Size: 120 119 9 309 6 15 Fragments arranged by size: 799 442 364 309 239 141 120 119 114 104 60 15 9 6 Bpu10I CC'TnA_GC Cuts at: 0 506 901 1038 1900 2108 2132 2392 2470 Size: 506 395 137 862 208 24 260 78 Cuts at: 2470 2475 2841 Size: 5 366 Fragments arranged by size: 862 506 395 366 260 208 137 78 24 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 1141 1801 2841 Size: 145 996 660 1040 Fragments arranged by size: 1040 996 660 145 BsaI GGTCTCn'nnnn_ Cuts at: 0 1672 2841 Size: 1672 1169 BsaAI yAC'GTr Cuts at: 0 714 2841 Size: 714 2127 BsaHI Gr'CG_yC Cuts at: 0 85 557 1544 1721 2841 Size: 85 472 987 177 1120 Fragments arranged by size: 1120 987 472 177 85 BsaWI w'CCGG_w Cuts at: 0 1692 2841 Size: 1692 1149 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 529 559 1934 1964 2392 2422 2841 Size: 529 30 1375 30 428 30 419 Fragments arranged by size: 1375 529 428 419 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1330 2125 2135 2171 2218 2380 2841 Size: 1330 795 10 36 47 162 461 Fragments arranged by size: 1330 795 461 162 47 36 10 BseYI C'CCAG_C Cuts at: 0 307 958 1108 1704 1938 2063 2266 2348 Size: 307 651 150 596 234 125 203 82 Cuts at: 2348 2682 2841 Size: 334 159 Fragments arranged by size: 651 596 334 307 234 203 159 150 125 82 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 504 553 709 1352 1744 2602 2841 Size: 504 49 156 643 392 858 239 Fragments arranged by size: 858 643 504 392 239 156 49 BsiEI CG_ry'CG Cuts at: 0 655 2841 Size: 655 2186 BsiHKAI G_wGCw'C Cuts at: 0 112 451 1039 1244 1565 2476 2773 2841 Size: 112 339 588 205 321 911 297 68 Fragments arranged by size: 911 588 339 321 297 205 112 68 Bsp1286I G_dGCh'C Cuts at: 0 33 112 145 196 451 959 981 1039 Size: 33 79 33 51 255 508 22 58 Cuts at: 1039 1093 1109 1244 1476 1565 1913 1962 1990 Size: 54 16 135 232 89 348 49 28 Cuts at: 1990 2091 2099 2130 2162 2476 2773 2841 Size: 101 8 31 32 314 297 68 Fragments arranged by size: 508 348 314 297 255 232 135 101 89 79 68 58 54 51 49 33 33 32 31 28 22 16 8 BspEI T'CCGG_A Cuts at: 0 1692 2841 Size: 1692 1149 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2624 2771 2841 Size: 2624 147 70 Fragments arranged by size: 2624 147 70 BsrBI CCG'CTC Cuts at: 0 1809 2280 2841 Size: 1809 471 561 Fragments arranged by size: 1809 561 471 BsrDI GCAATG_nn' Cuts at: 0 706 2841 Size: 706 2135 BsrFI r'CCGG_y Cuts at: 0 654 2841 Size: 654 2187 BssSI C'ACGA_G Cuts at: 0 971 2385 2841 Size: 971 1414 456 Fragments arranged by size: 1414 971 456 BstAPI GCAn_nnn'nTGC Cuts at: 0 1020 1715 2307 2841 Size: 1020 695 592 534 Fragments arranged by size: 1020 695 592 534 BstEII G'GTnAC_C Cuts at: 0 2703 2841 Size: 2703 138 BstXI CCAn_nnnn'nTGG Cuts at: 0 360 2841 Size: 360 2481 BstYI r'GATC_y Cuts at: 0 167 347 1096 1850 2678 2841 Size: 167 180 749 754 828 163 Fragments arranged by size: 828 754 749 180 167 163 BtgI C'CryG_G Cuts at: 0 131 2841 Size: 131 2710 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 225 288 1589 2640 2841 Size: 225 63 1301 1051 201 Fragments arranged by size: 1301 1051 225 201 63 BtsI GCAGTG_nn' Cuts at: 0 11 50 2773 2841 Size: 11 39 2723 68 Fragments arranged by size: 2723 68 39 11 DraIII CAC_nnn'GTG Cuts at: 0 1923 2841 Size: 1923 918 EaeI y'GGCC_r Cuts at: 0 482 652 1067 1605 2101 2820 2841 Size: 482 170 415 538 496 719 21 Fragments arranged by size: 719 538 496 482 415 170 21 EagI C'GGCC_G Cuts at: 0 652 2841 Size: 652 2189 EarI CTCTTCn'nnn_ Cuts at: 0 133 439 1978 2841 Size: 133 306 1539 863 Fragments arranged by size: 1539 863 306 133 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1201 1826 2841 Size: 1201 625 1015 Fragments arranged by size: 1201 1015 625 EcoICRI GAG'CTC Cuts at: 0 110 449 1037 2474 2841 Size: 110 339 588 1437 367 Fragments arranged by size: 1437 588 367 339 110 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 619 799 859 973 1077 1519 1758 2002 Size: 619 180 60 114 104 442 239 244 Cuts at: 2002 2122 2263 2383 2502 2511 2679 2820 2826 Size: 120 141 120 119 9 168 141 6 Cuts at: 2826 2841 Size: 15 Fragments arranged by size: 619 442 244 239 180 168 141 141 120 120 119 114 104 60 15 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 1489 2841 Size: 1489 1352 EcoO109I rG'GnC_Cy Cuts at: 0 500 645 745 1384 1473 1494 2087 2088 Size: 500 145 100 639 89 21 593 1 Cuts at: 2088 2095 2096 2841 Size: 7 1 745 Fragments arranged by size: 745 639 593 500 145 100 89 21 7 1 1 HaeII r_GCGC'y Cuts at: 0 560 2841 Size: 560 2281 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 327 359 529 561 659 691 1172 1204 Size: 327 32 170 32 98 32 481 32 Cuts at: 1204 2392 2424 2841 Size: 1188 32 417 Fragments arranged by size: 1188 481 417 327 170 98 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1395 2841 Size: 1395 1446 HindIII A'AGCT_T Cuts at: 0 121 2841 Size: 121 2720 KasI G'GCGC_C Cuts at: 0 556 2841 Size: 556 2285 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 379 2396 2841 Size: 379 2017 445 Fragments arranged by size: 2017 445 379 MscI TGG'CCA Cuts at: 0 484 1069 1607 2822 2841 Size: 484 585 538 1215 19 Fragments arranged by size: 1215 585 538 484 19 MslI CAynn'nnrTG Cuts at: 0 529 1402 2740 2841 Size: 529 873 1338 101 Fragments arranged by size: 1338 873 529 101 MspA1I CmG'CkG Cuts at: 0 226 962 1437 1745 1933 2107 2316 2522 Size: 226 736 475 308 188 174 209 206 Cuts at: 2522 2686 2826 2841 Size: 164 140 15 Fragments arranged by size: 736 475 308 226 209 206 188 174 164 140 15 NaeI GCC'GGC Cuts at: 0 656 2841 Size: 656 2185 NarI GG'CG_CC Cuts at: 0 557 2841 Size: 557 2284 NdeI CA'TA_TG Cuts at: 0 564 2841 Size: 564 2277 NgoMIV G'CCGG_C Cuts at: 0 654 2841 Size: 654 2187 NspI r_CATG'y Cuts at: 0 1018 1331 1767 2841 Size: 1018 313 436 1074 Fragments arranged by size: 1074 1018 436 313 PasI CC'CwG_GG Cuts at: 0 1428 2092 2841 Size: 1428 664 749 Fragments arranged by size: 1428 749 664 PflMI CCAn_nnn'nTGG Cuts at: 0 613 1076 1497 2841 Size: 613 463 421 1344 Fragments arranged by size: 1344 613 463 421 PfoI T'CCnGG_A Cuts at: 0 2294 2488 2624 2841 Size: 2294 194 136 217 Fragments arranged by size: 2294 217 194 136 PmlI CAC'GTG Cuts at: 0 714 2841 Size: 714 2127 PpuMI rG'GwC_Cy Cuts at: 0 500 2841 Size: 500 2341 PspOMI G'GGCC_C Cuts at: 0 1472 2087 2095 2841 Size: 1472 615 8 746 Fragments arranged by size: 1472 746 615 8 PstI C_TGCA'G Cuts at: 0 1932 2312 2691 2768 2841 Size: 1932 380 379 77 73 Fragments arranged by size: 1932 380 379 77 73 PvuII CAG'CTG Cuts at: 0 226 962 1437 1745 2107 2316 2522 2686 Size: 226 736 475 308 362 209 206 164 Cuts at: 2686 2826 2841 Size: 140 15 Fragments arranged by size: 736 475 362 308 226 209 206 164 140 15 SacI G_AGCT'C Cuts at: 0 112 451 1039 2476 2841 Size: 112 339 588 1437 365 Fragments arranged by size: 1437 588 365 339 112 SapI GCTCTTCn'nnn_ Cuts at: 0 133 439 1978 2841 Size: 133 306 1539 863 Fragments arranged by size: 1539 863 306 133 SfcI C'TryA_G Cuts at: 0 371 777 1928 1963 2308 2687 2764 2841 Size: 371 406 1151 35 345 379 77 77 Fragments arranged by size: 1151 406 379 371 345 77 77 35 SfoI GGC'GCC Cuts at: 0 558 2841 Size: 558 2283 SmaI CCC'GGG Cuts at: 0 1102 2542 2841 Size: 1102 1440 299 Fragments arranged by size: 1440 1102 299 SmlI C'TyrA_G Cuts at: 0 124 1156 1816 2841 Size: 124 1032 660 1025 Fragments arranged by size: 1032 1025 660 124 SphI G_CATG'C Cuts at: 0 1018 2841 Size: 1018 1823 StuI AGG'CCT Cuts at: 0 611 2446 2841 Size: 611 1835 395 Fragments arranged by size: 1835 611 395 StyI C'CwwG_G Cuts at: 0 74 188 200 479 612 1387 1724 1990 Size: 74 114 12 279 133 775 337 266 Cuts at: 1990 2083 2841 Size: 93 758 Fragments arranged by size: 775 758 337 279 266 133 114 93 74 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1362 2841 Size: 1362 1479 TatI w'GTAC_w Cuts at: 0 320 1478 2841 Size: 320 1158 1363 Fragments arranged by size: 1363 1158 320 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1934 1966 2841 Size: 1934 32 875 Fragments arranged by size: 1934 875 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2526 2841 Size: 2526 315 XmaI C'CCGG_G Cuts at: 0 1100 2540 2841 Size: 1100 1440 301 Fragments arranged by size: 1440 1100 301 XmnI GAAnn'nnTTC Cuts at: 0 1304 2841 Size: 1304 1537 ZraI GAC'GTC Cuts at: 0 1545 2841 Size: 1545 1296 Enzymes that do cut: AarI AatII AccI AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BclI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SapI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AhdI AleI AloI AscI AseI AsiSI AvrII BaeI BamHI BciVI BfrBI BglI BglII BmtI BsaBI BsiWI BsmI BsmBI BspHI BsrGI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI FspI FspAI HpaI KpnI MfeI MluI NcoI NheI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI