Restriction map for
pFN21AE6426
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AhdI Alw44I ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BalI
BbeI BbrPI BbsI BcgI BfaI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaI BseAI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
BspEI BssSI Bst1107I BstBI BstZ17I
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I EcoNI EcoRI EcoRV
FseI FspI HincII HindII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MseI MunI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tru9I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 1023 GTAC GT^AC
AflIII 569 ACATGT A^C[GA][CT]GT
AgeI 36 ACCGGT A^CCGGT
Aor51HI 1055 AGCGCT AGC^GCT
ApaI 1390 GGGCCC GGGCC^C
AspI 802 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1305 GGATCC G^GATCC
BanI 1037 GGCACC G^G[CT][GA]CC
BbvCI 338 CCTCAGC CCTCAGC(-5/-2)
BciVI 404 GGATAC GTATCC(6/5)
BcnI 813 CCGGG CC^[GC]GG
BseRI 211 GAGGAG GAGGAG(10/8)
Bsp1407I 1022 TGTACA T^GTACA
BspDI 1264 ATCGAT AT^CGAT
BspHI 590 TCATGA T^CATGA
BspLU11I 569 ACATGT A^CATGT
BspMI 1255 ACCTGC ACCTGC(4/8)
BsrBI 817 GAGCGG CCGCTC(-3/-3)
BsrGI 1022 TGTACA T^GTACA
BssHII 1518 GCGCGC G^CGCGC
BstAPI 1250 GCATTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1305 GGATCC [GA]^GATC[CT]
ClaI 1264 ATCGAT AT^CGAT
DrdI 231 GACCCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 367 CTCTTC CTCTTC(1/4)
Eco47III 1055 AGCGCT AGC^GCT
EcoT22I 615 ATGCAT ATGCA^T
HindIII 1563 AAGCTT A^AGCTT
Ksp632I 367 CTCTTC CTCTTC(1/4)
MflI 1305 GGATCC [GA]^GATC[CT]
NaeI 137 GCCGGC GCC^GGC
NciI 813 CCGGG CC^[GC]GG
NgoMIV 137 GCCGGC G^CCGGC
NsiI 615 ATGCAT ATGCA^T
NspI 569 ACATGT [GA]CATG^[CT]
PflFI 802 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 720 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 36 ACCGGT A^CCGGT
RcaI 590 TCATGA T^CATGA
RsaI 1023 GTAC GT^AC
SacI 679 GAGCTC GAGCT^C
SspBI 1022 TGTACA T^GTACA
Tsp509I 1508 AATT ^AATT
Tth111I 802 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 720 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 163 CCAAAACGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1305 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 605 GTCTAC GT^[AC][GT]AC
1315 GTCTAC
AccII 1093 CGCG CG^CG
1519 CGCG
AcyI 453 GACGCC G[GA]^CG[CT]C
821 GGCGTC
AocI 1164 CCTGAGG CC^T[ACGT]AGG
1309 CCTGAGG
AspHI 577 GAGCAC G[AT]GC[AT]^C
679 GAGCTC
BclI 197 TGATCA T^GATCA
1511 TGATCA
BlpI 9 GCTGAGC GC^T[ACGT]AGC
909 GCTGAGC
BpmI 142 CTCCAG CTGGAG(16/14)
718 CTCCAG
Bpu1102I 9 GCTGAGC GC^T[ACGT]AGC
909 GCTGAGC
BsaHI 453 GACGCC G[GA]^CG[CT]C
821 GGCGTC
BsgI 1203 CTGCAC GTGCAG(16/14)
1459 CTGCAC
BsiHKAI 577 GAGCAC G[AT]GC[AT]^C
679 GAGCTC
BsrDI 348 CATTGC GCAATG(2/0)
43 GCAATG
BsrFI 36 ACCGGT [GA]^CCGG[CT]
137 GCCGGC
BstEII 184 GGTCACC G^GT[ACGT]ACC
1358 GGTCACC
BstPI 184 GGTCACC G^GT[ACGT]ACC
1358 GGTCACC
BstUI 1093 CGCG CG^CG
1519 CGCG
Bsu36I 1164 CCTGAGG CC^T[ACGT]AGG
1309 CCTGAGG
CelII 9 GCTGAGC GC^T[ACGT]AGC
909 GCTGAGC
Cfr10I 36 ACCGGT [GA]^CCGG[CT]
137 GCCGGC
Eco81I 1164 CCTGAGG CC^T[ACGT]AGG
1309 CCTGAGG
EcoO65I 184 GGTCACC G^GT[ACGT]ACC
1358 GGTCACC
FbaI 197 TGATCA T^GATCA
1511 TGATCA
HaeII 761 GGCGCT [GA]GCGC^[CT]
1055 AGCGCT
Hin1I 453 GACGCC G[GA]^CG[CT]C
821 GGCGTC
HinfI 226 GACTC G^A[ACGT]TC
1235 GAGTC
MspA1I 341 CAGCTG C[AC]G^C[GT]G
1129 CAGCTG
MvnI 1093 CGCG CG^CG
1519 CGCG
NcoI 52 CCATGG C^CATGG
1421 CCATGG
PleI 226 GACTC GAGTC(4/5)
1235 GAGTC
PpuMI 276 GGGTCCC [GA]G^G[AT]CC[CT]
1016 GGGTCCT
PvuII 341 CAGCTG CAG^CTG
1129 CAGCTG
SfcI 206 CTACAG C^T[GA][CT]AG
607 CTACAG
TaqI 229 TCGA T^CGA
1265 TCGA
TthHB8I 229 TCGA T^CGA
1265 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbvI BmyI BsaJI BsaWI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI MaeIII MboI MboII MnlI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I TspRI