(Circular) (Six-base) MAPSORT of: pFN21AE6426.seq Check: 7247 from: 1 to: 6277 pFN21AE6426 6277 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4087 278 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2611 3321 3620 1937 Size: 674 710 299 4594 Fragments arranged by size: 4594 710 674 299 Acc65I G'GTAC_C Cuts at: 3598 3598 Size: 6277 AclI AA'CG_TT Cuts at: 1563 4404 4777 1563 Size: 2841 373 3063 Fragments arranged by size: 3063 2841 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2408 2504 2611 3110 4340 5724 5992 1632 Size: 776 96 107 499 1230 1384 268 1917 Fragments arranged by size: 1917 1384 1230 776 499 268 107 96 AfeI AGC'GCT Cuts at: 3062 3062 Size: 6277 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6080 AflIII A'CryG_T Cuts at: 1203 2574 5176 6153 1203 Size: 1371 2602 977 1327 Fragments arranged by size: 2602 1371 1327 977 AgeI A'CCGG_T Cuts at: 2041 5108 2041 Size: 3067 3210 AhdI GACnn_n'nnGTC Cuts at: 5006 5006 Size: 6277 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6277 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6277 AlwNI CAG_nnn'CTG Cuts at: 2545 2781 2810 2969 3405 4020 5592 2545 Size: 236 29 159 436 615 1572 3230 Fragments arranged by size: 3230 1572 615 436 236 159 29 ApaI G_GGCC'C Cuts at: 3399 3399 Size: 6277 ApaLI G'TGCA_C Cuts at: 4334 5490 4334 Size: 1156 5121 ApoI r'AATT_y Cuts at: 1493 3586 3812 3964 6073 1493 Size: 2093 226 152 2109 1697 Fragments arranged by size: 2109 2093 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4831 160 Size: 872 3799 1606 Fragments arranged by size: 3799 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 AvaI C'yCGr_G Cuts at: 1870 1943 3602 1870 Size: 73 1659 4545 Fragments arranged by size: 4545 1659 73 BamHI G'GATC_C Cuts at: 3310 3607 3974 3310 Size: 297 367 5613 Fragments arranged by size: 5613 367 297 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3042 3598 5053 618 Size: 325 217 627 1255 556 1455 1842 Fragments arranged by size: 1842 1455 1255 627 556 325 217 BanII G_rGCy'C Cuts at: 1391 2087 2688 3399 3498 3558 3596 1391 Size: 696 601 711 99 60 38 4072 Fragments arranged by size: 4072 711 696 601 99 60 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5306 BbvCI CC'TCA_GC Cuts at: 2344 2344 Size: 6277 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4468 4502 1903 Size: 34 2531 34 3678 Fragments arranged by size: 3678 2531 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2403 4169 5379 2403 Size: 1766 1210 3301 Fragments arranged by size: 3301 1766 1210 BclI T'GATC_A Cuts at: 1548 2202 3516 1548 Size: 654 1314 4309 Fragments arranged by size: 4309 1314 654 BfrBI ATG'CAT Cuts at: 2622 2622 Size: 6277 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4888 136 Size: 107 122 71 631 605 3216 1525 Fragments arranged by size: 3216 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6272 6272 Size: 6277 BlpI GC'TnA_GC Cuts at: 2015 2915 3694 2015 Size: 900 779 4598 Fragments arranged by size: 4598 900 779 Bme1580I G_kGCm'C Cuts at: 1790 2695 3399 4338 5494 1790 Size: 905 704 939 1156 2573 Fragments arranged by size: 2573 1156 939 905 704 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6277 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2652 4008 4966 Size: 416 53 83 399 24 1235 1356 958 Cuts at: 4966 442 Size: 1753 Fragments arranged by size: 1753 1356 1235 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6277 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3384 3384 Size: 6277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2708 4937 1239 Size: 893 576 2229 2579 Fragments arranged by size: 2579 2229 893 576 Bpu10I CC'TnA_GC Cuts at: 2344 2344 Size: 6277 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4402 5267 5565 4402 Size: 865 298 5114 Fragments arranged by size: 5114 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4940 882 Size: 617 3441 2219 Fragments arranged by size: 3441 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4068 493 Size: 711 2864 2702 Fragments arranged by size: 2864 2702 711 BsaBI GATnn'nnATC Cuts at: 6271 6271 Size: 6277 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2459 2827 4084 4466 Size: 53 83 186 936 926 368 1257 382 Cuts at: 4466 5101 275 Size: 635 1451 Fragments arranged by size: 1451 1257 936 926 635 382 368 186 83 53 BsaWI w'CCGG_w Cuts at: 2041 2325 3471 4710 5108 5382 5529 5940 2041 Size: 284 1146 1239 398 274 147 411 2378 Fragments arranged by size: 2378 1239 1146 411 398 284 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6052 Fragments arranged by size: 6052 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 1186 Size: 1045 5232 BseYI C'CCAG_C Cuts at: 2029 2278 2398 3132 3184 5480 5976 2029 Size: 249 120 734 52 2296 496 2330 Fragments arranged by size: 2330 2296 734 496 249 120 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3193 3449 1163 Size: 573 1457 256 3991 Fragments arranged by size: 3991 1457 573 256 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3727 4488 5516 1540 Size: 275 186 1726 761 1028 2301 Fragments arranged by size: 2301 1726 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2586 2688 4338 4423 5494 2586 Size: 102 1650 85 1071 3369 Fragments arranged by size: 3369 1650 1071 102 85 BsmI GAATG_Cn' Cuts at: 3788 3881 3788 Size: 93 6184 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2586 2688 2695 3399 3498 3558 Size: 399 297 499 102 7 704 99 60 Cuts at: 3558 3596 4338 4423 5494 1391 Size: 38 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 704 499 399 297 102 99 85 60 38 7 BspEI T'CCGG_A Cuts at: 5940 5940 Size: 6277 BspHI T'CATG_A Cuts at: 2595 4166 5896 2595 Size: 1571 1730 2976 Fragments arranged by size: 2976 1730 1571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3269 3565 3632 844 Size: 2425 296 67 3489 Fragments arranged by size: 3489 2425 296 67 BsrBI CCG'CTC Cuts at: 1394 1823 2824 4164 1394 Size: 429 1001 1340 3507 Fragments arranged by size: 3507 1340 1001 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2055 2352 4772 4946 65 Size: 1183 807 297 2420 174 1396 Fragments arranged by size: 2420 1396 1183 807 297 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2041 2142 4921 5108 1700 Size: 110 231 101 2779 187 2869 Fragments arranged by size: 2869 2779 231 187 110 101 BsrGI T'GTAC_A Cuts at: 96 3027 96 Size: 2931 3346 BssHII G'CGCG_C Cuts at: 1925 3523 1925 Size: 1598 4679 BssSI C'ACGA_G Cuts at: 4337 5349 4337 Size: 1012 5265 BstAPI GCAn_nnn'nTGC Cuts at: 794 3261 794 Size: 2467 3810 BstBI TT'CG_AA Cuts at: 5081 5081 Size: 6277 BstEII G'GTnAC_C Cuts at: 1184 2189 3363 1184 Size: 1005 1174 4098 Fragments arranged by size: 4098 1174 1005 BstXI CCAn_nnnn'nTGG Cuts at: 2680 2890 3303 2680 Size: 210 413 5654 Fragments arranged by size: 5654 413 210 BstYI r'GATC_y Cuts at: 1972 3310 3607 3974 4359 4376 5828 5914 5926 Size: 1338 297 367 385 17 1452 86 12 Cuts at: 5926 6272 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1338 385 367 346 297 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3170 3315 3170 Size: 145 6132 BtgI C'CryG_G Cuts at: 513 1065 2057 3000 3426 513 Size: 552 992 943 426 3364 Fragments arranged by size: 3364 992 943 552 426 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5538 Fragments arranged by size: 5538 683 56 BtsI GCAGTG_nn' Cuts at: 3793 4587 4607 3793 Size: 794 20 5463 Fragments arranged by size: 5463 794 20 ClaI AT'CG_AT Cuts at: 1554 3270 1554 Size: 1716 4561 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6242 DraI TTT'AAA Cuts at: 3581 3581 Size: 6277 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2242 5284 809 Size: 507 926 3042 1802 Fragments arranged by size: 3042 1802 926 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2247 3003 3235 Size: 54 1425 50 275 18 417 756 232 Cuts at: 3235 3305 3724 4613 6242 8 Size: 70 419 889 1629 43 Fragments arranged by size: 1629 1425 889 756 419 417 275 232 70 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3724 1537 Size: 275 1912 4090 Fragments arranged by size: 4090 1912 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2378 4207 1353 Size: 1025 1829 3423 Fragments arranged by size: 3423 1829 1025 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3446 4864 5238 5384 387 Size: 1064 138 1857 1418 374 146 1280 Fragments arranged by size: 1857 1418 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2686 2686 Size: 6277 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2408 2504 2611 2708 3110 4340 Size: 393 500 276 96 107 97 402 1230 Cuts at: 4340 4937 5724 5992 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1230 787 597 500 402 393 276 268 107 97 96 EcoO109I rG'GnC_Cy Cuts at: 2282 2778 3022 3395 2282 Size: 496 244 373 5164 Fragments arranged by size: 5164 496 373 244 EcoRI G'AATT_C Cuts at: 3586 3964 3586 Size: 378 5899 FspI TGC'GCA Cuts at: 4783 4783 Size: 6277 HaeII r_GCGC'y Cuts at: 1678 2770 3064 5424 1678 Size: 1092 294 2360 2531 Fragments arranged by size: 2531 2360 1092 294 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3163 3195 4918 4950 4992 Size: 32 135 32 1591 32 1723 32 42 Cuts at: 4992 5024 1373 Size: 32 2626 Fragments arranged by size: 2626 1723 1591 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3621 3867 669 Size: 988 281 1683 246 3079 Fragments arranged by size: 3079 1683 988 281 246 HindIII A'AGCT_T Cuts at: 748 3568 748 Size: 2820 3457 HpaI GTT'AAC Cuts at: 3867 3867 Size: 6277 KpnI G_GTAC'C Cuts at: 3602 3602 Size: 6277 MfeI C'AATT_G Cuts at: 3876 3876 Size: 6277 MluI A'CGCG_T Cuts at: 6153 6153 Size: 6277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2429 5391 5575 1305 Size: 1124 2962 184 2007 Fragments arranged by size: 2962 2007 1124 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4455 Fragments arranged by size: 4455 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2903 2978 3155 4235 4594 4753 518 Size: 648 1737 75 177 1080 359 159 2042 Fragments arranged by size: 2042 1737 1080 648 359 177 159 75 MspA1I CmG'CkG Cuts at: 1606 2348 3136 3693 4370 5518 5763 1606 Size: 742 788 557 677 1148 245 2120 Fragments arranged by size: 2120 1148 788 742 677 557 245 NaeI GCC'GGC Cuts at: 2144 2144 Size: 6277 NcoI C'CATG_G Cuts at: 513 1065 2057 3426 513 Size: 552 992 1369 3364 Fragments arranged by size: 3364 1369 992 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6277 NgoMIV G'CCGG_C Cuts at: 2142 2142 Size: 6277 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6277 NotI GC'GGCC_GC Cuts at: 3724 3724 Size: 6277 NsiI A_TGCA'T Cuts at: 2624 2624 Size: 6277 NspI r_CATG'y Cuts at: 1132 1229 2578 3635 5180 1132 Size: 97 1349 1057 1545 2229 Fragments arranged by size: 2229 1545 1349 1057 97 PasI CC'CwG_GG Cuts at: 1348 2260 1348 Size: 912 5365 PciI A'CATG_T Cuts at: 2574 5176 2574 Size: 2602 3675 PflMI CCAn_nnn'nTGG Cuts at: 1108 2731 1108 Size: 1623 4654 PfoI T'CCnGG_A Cuts at: 2817 2817 Size: 6277 PmeI GTTT'AAAC Cuts at: 3581 3581 Size: 6277 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4346 4378 5859 5891 4346 Size: 32 1481 32 4732 Fragments arranged by size: 4732 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2282 3022 2282 Size: 740 5537 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6277 PsiI TTA'TAA Cuts at: 3847 3847 Size: 6277 PspOMI G'GGCC_C Cuts at: 3395 3395 Size: 6277 PstI C_TGCA'G Cuts at: 830 3629 830 Size: 2799 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6277 PvuII CAG'CTG Cuts at: 2348 3136 2348 Size: 788 5489 SacI G_AGCT'C Cuts at: 2688 2688 Size: 6277 SalI G'TCGA_C Cuts at: 1936 3619 1936 Size: 1683 4594 SanDI GG'GwC_CC Cuts at: 2282 2282 Size: 6277 SbfI CC_TGCA'GG Cuts at: 3629 3629 Size: 6277 ScaI AGT'ACT Cuts at: 4525 6237 4525 Size: 1712 4565 SfcI C'TryA_G Cuts at: 826 1045 2211 2612 3625 4760 5441 5632 826 Size: 219 1166 401 1013 1135 681 191 1471 Fragments arranged by size: 1471 1166 1135 1013 681 401 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6277 SmaI CCC'GGG Cuts at: 1872 3604 1872 Size: 1732 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4381 5282 5544 820 Size: 197 926 2438 901 262 1553 Fragments arranged by size: 2438 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4068 493 Size: 3575 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6277 SphI G_CATG'C Cuts at: 1132 3635 1132 Size: 2503 3774 SspI AAT'ATT Cuts at: 5 52 4201 5 Size: 47 4149 2081 Fragments arranged by size: 4149 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6277 StyI C'CwwG_G Cuts at: 513 1065 2057 2361 2601 3426 3716 513 Size: 552 992 304 240 825 290 3074 Fragments arranged by size: 3074 992 825 552 304 290 240 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2374 3060 3227 4304 4321 4474 4659 Size: 1255 794 686 167 1077 17 153 185 Cuts at: 4659 5104 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1077 794 686 445 185 167 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3027 4523 6235 Size: 274 80 33 51 1443 1050 1496 1712 Cuts at: 6235 96 Size: 138 Fragments arranged by size: 1712 1496 1443 1050 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6237 6269 6237 Size: 32 6245 Tth111I GACn'n_nGTC Cuts at: 1316 2810 1316 Size: 1494 4783 XbaI T'CTAG_A Cuts at: 3613 3613 Size: 6277 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2175 2175 Size: 6277 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6277 XmaI C'CCGG_G Cuts at: 1870 3602 1870 Size: 1732 4545 XmnI GAAnn'nnTTC Cuts at: 4406 4406 Size: 6277 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4085 276 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI