################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaHI BsaI BsaWI BseAI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI Hin1I HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MslI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 459 AAATTC [GA]^AATT[CT] ApoI 459 AAATTC [GA]^AATT[CT] Asp700I 484 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 968 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 905 TGCGCA TGC^GCA BanI 100 GGTGCC G^G[CT][GA]CC BbsI 1507 GAAGAC GAAGAC(2/6) BcgI 1268 GCATGACCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 1507 GAAGAC GAAGAC(2/6) BsaAI 262 TACGTG [CT]AC^GT[GA] BsaBI 699 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 657 CGGCCG CG[GA][CT]^CG BspHI 1221 TCATGA T^CATGA BspMI 68 ACCTGC ACCTGC(4/8) BsrBI 417 GAGCGG CCGCTC(-3/-3) BstBI 404 TTCGAA TT^CGAA DdeI 34 CTCAG C^T[ACGT]AG DraI 373 TTTAAA TTT^AAA DraII 815 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 1556 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 657 CGGCCG C^GGCCG EarI 132 GAAGAG CTCTTC(1/4) EclXI 657 CGGCCG C^GGCCG Eco52I 657 CGGCCG C^GGCCG EcoO109I 815 AGGCCCC [GA]G^G[ACGT]CC[CT] FspI 905 TGCGCA TGC^GCA HgaI 735 GCGTC GACGC(5/10) HincII 691 GTCAAC GT[CT]^[GA]AC HindII 691 GTCAAC GT[CT]^[GA]AC HindIII 1475 AAGCTT A^AGCTT Ksp632I 132 GAAGAG CTCTTC(1/4) MamI 699 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NspI 1815 ACATGC [GA]CATG^[CT] NspV 404 TTCGAA TT^CGAA PflFI 968 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1410 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1221 TCATGA T^CATGA ScaI 1467 AGTACT AGT^ACT SfuI 404 TTCGAA TT^CGAA SmlI 979 CTTGAG C^T[CT][GA]AG Tth111I 968 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 484 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AflIII 263 ACGTGT A^C[GA][CT]GT 1074 ACGTGT AlwI 350 GATCC GGATC(4/5) 553 GATCC AlwNI 757 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1560 CAGTGACTG AvaI 836 CCCGAG C^[CT]CG[GA]G 877 CCCGAG BfaI 247 CTAG C^TAG 329 CTAG BsgI 70 CTGCAC GTGCAG(16/14) 425 GTGCAG BsmBI 736 CGTCTC CGTCTC(1/5) 653 GAGACG BsoBI 836 CCCGAG C^[CT]CG[GA]G 877 CCCGAG BsrDI 710 GCAATG GCAATG(2/0) 1148 GCAATG BsrFI 659 GCCGGC [GA]^CCGG[CT] 1135 GCCGGC BstYI 349 AGATCC [GA]^GATC[CT] 552 AGATCC Cfr10I 659 GCCGGC [GA]^CCGG[CT] 1135 GCCGGC DsaI 182 CCACGG C^C[GA][CT]GG 1600 CCACGG EaeI 312 TGGCCG [CT]^GGCC[GA] 657 CGGCCG EcoT14I 28 CCTTGG C^C[AT][AT]GG 493 CCAAGG FauI 1094 CCCGC CCCGC(4/6) 419 GCGGG HaeII 6 GGCGCT [GA]GCGC^[CT] 137 GGCGCT MaeI 247 CTAG C^TAG 329 CTAG MaeII 263 ACGT A^CGT 1074 ACGT MaeIII 1376 GTGAC ^GT[ACGT]AC 1562 GTGAC MflI 349 AGATCC [GA]^GATC[CT] 552 AGATCC NaeI 659 GCCGGC GCC^GGC 1135 GCCGGC NgoMIV 659 GCCGGC G^CCGGC 1135 GCCGGC PflMI 1542 CCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1559 CCAGTGACTGG PstI 206 CTGCAG CTGCA^G 1512 CTGCAG StyI 28 CCTTGG C^C[AT][AT]GG 493 CCAAGG Tsp45I 1376 GTGAC ^GT[GC]AC 1562 GTGAC Van91I 1542 CCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1559 CCAGTGACTGG XcmI 1538 CCAACCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1555 CCACCCAGTGACTGG XhoII 349 AGATCC [GA]^GATC[CT] 552 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AfaI AluI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I