################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII BalI BamHI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AflIII 770 ACATGT A^C[GA][CT]GT Alw44I 313 GTGCAC G^TGCAC AlwI 1263 GATCC GGATC(4/5) AocI 99 CCTGAGG CC^T[ACGT]AGG Aor51HI 1274 AGCGCT AGC^GCT ApaLI 313 GTGCAC G^TGCAC AspI 1054 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 75 CCTAGG C^CTAGG BbvCI 93 CCTCAGC CCTCAGC(-5/-2) BlnI 75 CCTAGG C^CTAGG BsaI 1705 GAGACC GGTCTC(1/5) BsmI 1140 GAATGC GAATGC(1/-1) BspLU11I 770 ACATGT A^CATGT BsrBI 1750 GAGCGG CCGCTC(-3/-3) BsrDI 1590 CATTGC GCAATG(2/0) Bsu36I 99 CCTGAGG CC^T[ACGT]AGG DraIII 127 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1274 AGCGCT AGC^GCT Eco81I 99 CCTGAGG CC^T[ACGT]AGG MaeIII 247 GTGAC ^GT[ACGT]AC MslI 1062 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1322 GCCGGC GCC^GGC NgoMIV 1322 GCCGGC G^CCGGC PflFI 1054 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC SmaI 863 CCCGGG CCC^GGG SmlI 252 CTTGAG C^T[CT][GA]AG SnaBI 508 TACGTA TAC^GTA SphI 548 GCATGC GCATG^C Tsp45I 247 GTGAC ^GT[GC]AC Tth111I 1054 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 863 CCCGGG C^CCGGG XmaI 863 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BbeI 1328 GGCGCC GGCGC^C 1402 GGCGCC BfaI 46 CTAG C^TAG 76 CTAG BsaAI 184 TACGTG [CT]AC^GT[GA] 508 TACGTA BseRI 1396 GAGGAG GAGGAG(10/8) 1567 GAGGAG BsgI 1189 CTGCAC GTGCAG(16/14) 470 GTGCAG BspMI 597 GCAGGT ACCTGC(4/8) 1065 GCAGGT BsrFI 809 GCCGGT [GA]^CCGG[CT] 1322 GCCGGC BssSI 847 CACGAG CACGAG(-5/-1) 979 CACGAG Cfr10I 809 GCCGGT [GA]^CCGG[CT] 1322 GCCGGC KasI 1328 GGCGCC G^GCGCC 1402 GGCGCC MaeI 46 CTAG C^TAG 76 CTAG NarI 1328 GGCGCC GG^CGCC 1402 GGCGCC SfoI 1328 GGCGCC GGC^GCC 1402 GGCGCC TaqI 620 TCGA T^CGA 1052 TCGA TthHB8I 620 TCGA T^CGA 1052 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI TspRI