(Circular) (Six-base) MAPSORT of: pFN21AE3918.seq Check: 9434 from: 1 to: 6526 pFN21AE3918 6526 bp With 182 enzymes: SgfI * March 28, 2012 11:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3061 3061 Size: 6526 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4336 278 Size: 53 83 186 936 2800 2468 Fragments arranged by size: 2800 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3869 1937 Size: 1932 4594 Acc65I G'GTAC_C Cuts at: 3847 3847 Size: 6526 AclI AA'CG_TT Cuts at: 1563 4653 5026 1563 Size: 3090 373 3063 Fragments arranged by size: 3090 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3128 3266 4589 5973 6241 1632 Size: 1496 138 1323 1384 268 1917 Fragments arranged by size: 1917 1496 1384 1323 268 138 AfeI AGC'GCT Cuts at: 3281 3281 Size: 6526 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6329 AflIII A'CryG_T Cuts at: 1203 2775 5425 6402 1203 Size: 1572 2650 977 1327 Fragments arranged by size: 2650 1572 1327 977 AgeI A'CCGG_T Cuts at: 5357 5357 Size: 6526 AhdI GACnn_n'nnGTC Cuts at: 5255 5255 Size: 6526 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6526 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2429 1184 Size: 1245 5281 AlwNI CAG_nnn'CTG Cuts at: 2344 2737 3700 4269 5841 2344 Size: 393 963 569 1572 3029 Fragments arranged by size: 3029 1572 963 569 393 ApaLI G'TGCA_C Cuts at: 2318 4583 5739 2318 Size: 2265 1156 3105 Fragments arranged by size: 3105 2265 1156 ApoI r'AATT_y Cuts at: 1493 3835 4061 4213 6322 1493 Size: 2342 226 152 2109 1697 Fragments arranged by size: 2342 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5080 160 Size: 872 4048 1606 Fragments arranged by size: 4048 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 AvaI C'yCGr_G Cuts at: 1870 1943 2644 2688 2868 3437 3752 3851 1870 Size: 73 701 44 180 569 315 99 4545 Fragments arranged by size: 4545 701 569 315 180 99 73 44 AvrII C'CTAG_G Cuts at: 2080 2080 Size: 6526 BamHI G'GATC_C Cuts at: 3856 4223 3856 Size: 367 6159 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 2296 2648 3333 3407 Size: 325 217 627 267 242 352 685 74 Cuts at: 3407 3847 5302 618 Size: 440 1455 1842 Fragments arranged by size: 1842 1455 685 627 440 352 325 267 242 217 74 BanII G_rGCy'C Cuts at: 1391 2043 2124 2925 2991 3129 3258 3512 3639 Size: 652 81 801 66 138 129 254 127 Cuts at: 3639 3845 1391 Size: 206 4072 Fragments arranged by size: 4072 801 652 254 206 138 129 127 81 66 BbeI G_GCGC'C Cuts at: 3337 3411 3337 Size: 74 6452 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5555 BbvCI CC'TCA_GC Cuts at: 2099 2099 Size: 6526 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4717 4751 1903 Size: 34 2780 34 3678 Fragments arranged by size: 3678 2780 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4418 5628 4418 Size: 1210 5316 BclI T'GATC_A Cuts at: 1548 1548 Size: 6526 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5137 136 Size: 107 122 71 631 605 3465 1525 Fragments arranged by size: 3465 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6521 6521 Size: 6526 BlpI GC'TnA_GC Cuts at: 3943 3943 Size: 6526 Bme1580I G_kGCm'C Cuts at: 1790 2301 2322 2689 2751 2861 3209 3469 3747 Size: 511 21 367 62 110 348 260 278 Cuts at: 3747 3774 4587 5743 1790 Size: 27 813 1156 2573 Fragments arranged by size: 2573 1156 813 511 367 348 278 260 110 62 27 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6526 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2714 4257 5215 Size: 416 53 83 399 24 1297 1543 958 Cuts at: 5215 442 Size: 1753 Fragments arranged by size: 1753 1543 1297 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6526 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2944 3526 2944 Size: 582 5944 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2027 2264 2564 2759 2773 3143 3248 3446 Size: 788 237 300 195 14 370 105 198 Cuts at: 3446 3710 3749 3755 5186 1239 Size: 264 39 6 1431 2579 Fragments arranged by size: 2579 1431 788 370 300 264 237 198 195 105 39 14 6 Bpu10I CC'TnA_GC Cuts at: 2099 2099 Size: 6526 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2278 4651 5516 5814 2278 Size: 2373 865 298 2990 Fragments arranged by size: 2990 2373 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3704 5189 882 Size: 617 2205 1485 2219 Fragments arranged by size: 2219 2205 1485 617 BsaAI yAC'GTr Cuts at: 493 1204 2191 2515 4317 493 Size: 711 987 324 1802 2702 Fragments arranged by size: 2702 1802 987 711 324 BsaBI GATnn'nnATC Cuts at: 6520 6520 Size: 6526 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2561 3334 3408 4333 Size: 53 83 186 936 1028 773 74 925 Cuts at: 4333 4715 5350 275 Size: 382 635 1451 Fragments arranged by size: 1451 1028 936 925 773 635 382 186 83 74 53 BsaWI w'CCGG_w Cuts at: 4959 5357 5631 5778 6189 4959 Size: 398 274 147 411 5296 Fragments arranged by size: 5296 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2624 2654 3199 3229 3566 Size: 30 165 30 1221 30 545 30 337 Cuts at: 3566 3596 1178 Size: 30 4108 Fragments arranged by size: 4108 1221 545 337 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3416 3587 1186 Size: 2230 171 4125 Fragments arranged by size: 4125 2230 171 BseYI C'CCAG_C Cuts at: 2266 2533 2725 2801 3649 5729 6225 2266 Size: 267 192 76 848 2080 496 2567 Fragments arranged by size: 2567 2080 848 496 267 192 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2496 3179 1163 Size: 573 760 683 4510 Fragments arranged by size: 4510 760 683 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3976 4737 5765 1540 Size: 275 186 1975 761 1028 2301 Fragments arranged by size: 2301 1975 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2124 2133 2322 2991 3129 3258 4587 4672 5743 Size: 9 189 669 138 129 1329 85 1071 Cuts at: 5743 2124 Size: 2907 Fragments arranged by size: 2907 1329 1071 669 189 138 129 85 9 BsmI GAATG_Cn' Cuts at: 3151 4037 4130 3151 Size: 886 93 5547 Fragments arranged by size: 5547 886 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2124 2133 2301 2322 2689 2751 Size: 399 253 81 9 168 21 367 62 Cuts at: 2751 2861 2925 2991 3129 3209 3258 3469 3512 Size: 110 64 66 138 80 49 211 43 Cuts at: 3512 3639 3747 3774 3845 4587 4672 5743 1391 Size: 127 108 27 71 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 367 253 211 168 138 127 110 108 85 81 80 71 66 64 62 49 43 27 21 9 BspEI T'CCGG_A Cuts at: 6189 6189 Size: 6526 BspHI T'CATG_A Cuts at: 4415 6145 4415 Size: 1730 4796 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2593 3061 3881 844 Size: 1749 468 820 3489 Fragments arranged by size: 3489 1749 820 468 BsrBI CCG'CTC Cuts at: 1394 1823 3757 4413 1394 Size: 429 1934 656 3507 Fragments arranged by size: 3507 1934 656 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3594 5021 5195 65 Size: 1183 2346 1427 174 1396 Fragments arranged by size: 2346 1427 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2814 3327 5170 5357 1700 Size: 110 1004 513 1843 187 2869 Fragments arranged by size: 2869 1843 1004 513 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6526 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6526 BssSI C'ACGA_G Cuts at: 2852 2984 4586 5598 2852 Size: 132 1602 1012 3780 Fragments arranged by size: 3780 1602 1012 132 BstAPI GCAn_nnn'nTGC Cuts at: 794 3076 3283 3486 794 Size: 2282 207 203 3834 Fragments arranged by size: 3834 2282 207 203 BstBI TT'CG_AA Cuts at: 5330 5330 Size: 6526 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6526 BstXI CCAn_nnnn'nTGG Cuts at: 2359 2398 3121 2359 Size: 39 723 5764 Fragments arranged by size: 5764 723 39 BstYI r'GATC_y Cuts at: 1972 3856 4223 4608 4625 6077 6163 6175 6521 Size: 1884 367 385 17 1452 86 12 346 Cuts at: 6521 1972 Size: 1977 Fragments arranged by size: 1977 1884 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2105 2105 Size: 6526 BtgI C'CryG_G Cuts at: 513 1065 2358 3565 3660 3705 513 Size: 552 1293 1207 95 45 3334 Fragments arranged by size: 3334 1293 1207 552 95 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3799 487 Size: 683 56 2573 3214 Fragments arranged by size: 3214 2573 683 56 BtsI GCAGTG_nn' Cuts at: 4042 4836 4856 4042 Size: 794 20 5712 Fragments arranged by size: 5712 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6526 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6491 DraI TTT'AAA Cuts at: 3830 3830 Size: 6526 DraIII CAC_nnn'GTG Cuts at: 2137 2137 Size: 6526 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5533 809 Size: 507 4217 1802 Fragments arranged by size: 4217 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2965 3453 3758 Size: 54 1425 50 275 18 1135 488 305 Cuts at: 3758 3973 4862 6491 8 Size: 215 889 1629 43 Fragments arranged by size: 1629 1425 1135 889 488 305 275 215 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3973 1537 Size: 275 2161 4090 Fragments arranged by size: 4090 2161 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2996 3134 3314 4456 1353 Size: 1643 138 180 1142 3423 Fragments arranged by size: 3423 1643 1142 180 138 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3260 3278 5113 5487 5633 387 Size: 1064 138 1671 18 1835 374 146 1280 Fragments arranged by size: 1835 1671 1280 1064 374 146 138 18 EcoICRI GAG'CTC Cuts at: 2122 2989 3127 3256 2122 Size: 867 138 129 5392 Fragments arranged by size: 5392 867 138 129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2027 2264 2564 2759 2773 3128 3143 Size: 393 395 237 300 195 14 355 15 Cuts at: 3143 3248 3266 3446 3710 3749 3755 4589 5186 Size: 105 18 180 264 39 6 834 597 Cuts at: 5186 5973 6241 1239 Size: 787 268 1524 Fragments arranged by size: 1524 834 787 597 395 393 355 300 268 264 237 195 180 105 39 18 15 14 6 EcoNI CCTnn'n_nnAGG Cuts at: 2084 2170 2612 3296 2084 Size: 86 442 684 5314 Fragments arranged by size: 5314 684 442 86 EcoO109I rG'GnC_Cy Cuts at: 2109 2605 2734 3529 2109 Size: 496 129 795 5106 Fragments arranged by size: 5106 795 496 129 EcoRI G'AATT_C Cuts at: 3835 4213 3835 Size: 378 6148 FspI TGC'GCA Cuts at: 5032 5032 Size: 6526 HaeII r_GCGC'y Cuts at: 1678 3283 3337 3411 5673 1678 Size: 1605 54 74 2262 2531 Fragments arranged by size: 2531 2262 1605 74 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2488 2520 2624 2656 5167 Size: 32 135 32 916 32 104 32 2511 Cuts at: 5167 5199 5241 5273 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2511 916 135 104 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3870 4116 669 Size: 988 281 1932 246 3079 Fragments arranged by size: 3079 1932 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6526 HpaI GTT'AAC Cuts at: 4116 4116 Size: 6526 KasI G'GCGC_C Cuts at: 3333 3407 3333 Size: 74 6452 KpnI G_GTAC'C Cuts at: 3851 3851 Size: 6526 MfeI C'AATT_G Cuts at: 4125 4125 Size: 6526 MluI A'CGCG_T Cuts at: 6402 6402 Size: 6526 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2805 5640 5824 1305 Size: 1500 2835 184 2007 Fragments arranged by size: 2835 2007 1500 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4704 Fragments arranged by size: 4704 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3071 4484 4843 5002 518 Size: 648 1905 1413 359 159 2042 Fragments arranged by size: 2042 1905 1413 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2281 2344 2590 2599 2884 3010 3088 3697 Size: 675 63 246 9 285 126 78 609 Cuts at: 3697 3942 4619 5767 6012 1606 Size: 245 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 675 609 285 246 245 245 126 78 63 9 NaeI GCC'GGC Cuts at: 3329 3329 Size: 6526 NarI GG'CG_CC Cuts at: 3334 3408 3334 Size: 74 6452 NcoI C'CATG_G Cuts at: 513 1065 2358 3660 3705 513 Size: 552 1293 1302 45 3334 Fragments arranged by size: 3334 1302 1293 552 45 NdeI CA'TA_TG Cuts at: 387 387 Size: 6526 NgoMIV G'CCGG_C Cuts at: 3327 3327 Size: 6526 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6526 NotI GC'GGCC_GC Cuts at: 3973 3973 Size: 6526 NspI r_CATG'y Cuts at: 1132 1229 2416 2557 2779 3884 5429 1132 Size: 97 1187 141 222 1105 1545 2229 Fragments arranged by size: 2229 1545 1187 1105 222 141 97 PasI CC'CwG_GG Cuts at: 1348 2272 1348 Size: 924 5602 PciI A'CATG_T Cuts at: 2775 5425 2775 Size: 2650 3876 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6526 PfoI T'CCnGG_A Cuts at: 3498 3512 3498 Size: 14 6512 PmeI GTTT'AAAC Cuts at: 3830 3830 Size: 6526 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4595 4627 6108 6140 4595 Size: 32 1481 32 4981 Fragments arranged by size: 4981 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2605 2734 3529 2605 Size: 129 795 5602 Fragments arranged by size: 5602 795 129 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6526 PsiI TTA'TAA Cuts at: 4096 4096 Size: 6526 PstI C_TGCA'G Cuts at: 830 2385 2523 2604 3387 3812 3878 830 Size: 1555 138 81 783 425 66 3478 Fragments arranged by size: 3478 1555 783 425 138 81 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6526 PvuII CAG'CTG Cuts at: 2281 2344 2599 2884 3010 3088 3697 2281 Size: 63 255 285 126 78 609 5110 Fragments arranged by size: 5110 609 285 255 126 78 63 SacI G_AGCT'C Cuts at: 2124 2991 3129 3258 2124 Size: 867 138 129 5392 Fragments arranged by size: 5392 867 138 129 SalI G'TCGA_C Cuts at: 1936 3868 1936 Size: 1932 4594 SapI GCTCTTCn'nnn_ Cuts at: 2996 3134 3314 2996 Size: 138 180 6208 Fragments arranged by size: 6208 180 138 SbfI CC_TGCA'GG Cuts at: 3878 3878 Size: 6526 ScaI AGT'ACT Cuts at: 4774 6486 4774 Size: 1712 4814 SfcI C'TryA_G Cuts at: 826 1045 2324 2381 2519 2575 2600 3383 3808 Size: 219 1279 57 138 56 25 783 425 Cuts at: 3808 3874 5009 5690 5881 826 Size: 66 1135 681 191 1471 Fragments arranged by size: 1471 1279 1135 783 681 425 219 191 138 66 57 56 25 SfoI GGC'GCC Cuts at: 3335 3409 3335 Size: 74 6452 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6526 SmaI CCC'GGG Cuts at: 1872 2870 3853 1872 Size: 998 983 4545 Fragments arranged by size: 4545 998 983 SmlI C'TyrA_G Cuts at: 820 1017 1943 2257 4630 5531 5793 820 Size: 197 926 314 2373 901 262 1553 Fragments arranged by size: 2373 1553 926 901 314 262 197 SnaBI TAC'GTA Cuts at: 493 2515 4317 493 Size: 2022 1802 2702 Fragments arranged by size: 2702 2022 1802 SpeI A'CTAG_T Cuts at: 152 152 Size: 6526 SphI G_CATG'C Cuts at: 1132 2557 3884 1132 Size: 1425 1327 3774 Fragments arranged by size: 3774 1425 1327 SspI AAT'ATT Cuts at: 5 52 4450 5 Size: 47 4398 2081 Fragments arranged by size: 4398 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6526 StyI C'CwwG_G Cuts at: 513 1065 2080 2233 2352 2358 3138 3660 3705 Size: 552 1015 153 119 6 780 522 45 Cuts at: 3705 3965 513 Size: 260 3074 Fragments arranged by size: 3074 1015 780 552 522 260 153 119 45 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2968 4553 4570 4723 4908 5353 325 Size: 1255 1388 1585 17 153 185 445 1498 Fragments arranged by size: 1585 1498 1388 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2781 4772 6484 Size: 274 80 33 51 1443 804 1991 1712 Cuts at: 6484 96 Size: 138 Fragments arranged by size: 1991 1712 1443 804 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3199 3231 3332 3364 6486 6518 3199 Size: 32 101 32 3122 32 3207 Fragments arranged by size: 3207 3122 101 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3062 1316 Size: 1746 4780 XbaI T'CTAG_A Cuts at: 3862 3862 Size: 6526 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6526 XmaI C'CCGG_G Cuts at: 1870 2868 3851 1870 Size: 998 983 4545 Fragments arranged by size: 4545 998 983 XmnI GAAnn'nnTTC Cuts at: 4655 4655 Size: 6526 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4334 276 Size: 53 83 186 936 2800 2468 Fragments arranged by size: 2800 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI