(Linear) (Six-base) MAPSORT of: KIEE3918.seq Check: 5313 from: 1 to: 1821 KIEE3918.orf With 182 enzymes: SgfI * March 22, 2012 16:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1056 1821 Size: 1056 765 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1123 1261 1821 Size: 1123 138 560 Fragments arranged by size: 1123 560 138 AfeI AGC'GCT Cuts at: 0 1276 1821 Size: 1276 545 AflIII A'CryG_T Cuts at: 0 770 1821 Size: 770 1051 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 424 1821 Size: 424 1397 AlwNI CAG_nnn'CTG Cuts at: 0 339 732 1695 1821 Size: 339 393 963 126 Fragments arranged by size: 963 393 339 126 ApaLI G'TGCA_C Cuts at: 0 313 1821 Size: 313 1508 AvaI C'yCGr_G Cuts at: 0 639 683 863 1432 1747 1821 Size: 639 44 180 569 315 74 Fragments arranged by size: 639 569 315 180 74 44 AvrII C'CTAG_G Cuts at: 0 75 1821 Size: 75 1746 BanI G'GyrC_C Cuts at: 0 49 291 643 1328 1402 1821 Size: 49 242 352 685 74 419 Fragments arranged by size: 685 419 352 242 74 49 BanII G_rGCy'C Cuts at: 0 38 119 920 986 1124 1253 1507 1634 Size: 38 81 801 66 138 129 254 127 Cuts at: 1634 1821 Size: 187 Fragments arranged by size: 801 254 187 138 129 127 81 66 38 BbeI G_GCGC'C Cuts at: 0 1332 1406 1821 Size: 1332 74 415 Fragments arranged by size: 1332 415 74 BbvCI CC'TCA_GC Cuts at: 0 94 1821 Size: 94 1727 Bme1580I G_kGCm'C Cuts at: 0 296 317 684 746 856 1204 1464 1742 Size: 296 21 367 62 110 348 260 278 Cuts at: 1742 1769 1821 Size: 27 52 Fragments arranged by size: 367 348 296 278 260 110 62 52 27 21 BmrI ACTGGGnnnn_n' Cuts at: 0 709 1821 Size: 709 1112 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 939 1521 1821 Size: 939 582 300 Fragments arranged by size: 939 582 300 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 22 259 559 754 768 1138 1243 1441 Size: 22 237 300 195 14 370 105 198 Cuts at: 1441 1705 1744 1750 1821 Size: 264 39 6 71 Fragments arranged by size: 370 300 264 237 198 195 105 71 39 22 14 6 Bpu10I CC'TnA_GC Cuts at: 0 94 1821 Size: 94 1727 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 273 1821 Size: 273 1548 BsaI GGTCTCn'nnnn_ Cuts at: 0 1699 1821 Size: 1699 122 BsaAI yAC'GTr Cuts at: 0 186 510 1821 Size: 186 324 1311 Fragments arranged by size: 1311 324 186 BsaHI Gr'CG_yC Cuts at: 0 556 1329 1403 1821 Size: 556 773 74 418 Fragments arranged by size: 773 556 418 74 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 619 649 1194 1224 1561 1591 1821 Size: 619 30 545 30 337 30 230 Fragments arranged by size: 619 545 337 230 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1411 1582 1821 Size: 1411 171 239 Fragments arranged by size: 1411 239 171 BseYI C'CCAG_C Cuts at: 0 261 528 720 796 1644 1821 Size: 261 267 192 76 848 177 Fragments arranged by size: 848 267 261 192 177 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 491 1174 1821 Size: 491 683 647 Fragments arranged by size: 683 647 491 BsiHKAI G_wGCw'C Cuts at: 0 119 128 317 986 1124 1253 1821 Size: 119 9 189 669 138 129 568 Fragments arranged by size: 669 568 189 138 129 119 9 BsmI GAATG_Cn' Cuts at: 0 1146 1821 Size: 1146 675 Bsp1286I G_dGCh'C Cuts at: 0 38 119 128 296 317 684 746 856 Size: 38 81 9 168 21 367 62 110 Cuts at: 856 920 986 1124 1204 1253 1464 1507 1634 Size: 64 66 138 80 49 211 43 127 Cuts at: 1634 1742 1769 1821 Size: 108 27 52 Fragments arranged by size: 367 211 168 138 127 110 108 81 80 66 64 62 52 49 43 38 27 21 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 588 1056 1821 Size: 588 468 765 Fragments arranged by size: 765 588 468 BsrBI CCG'CTC Cuts at: 0 1752 1821 Size: 1752 69 BsrDI GCAATG_nn' Cuts at: 0 1589 1821 Size: 1589 232 BsrFI r'CCGG_y Cuts at: 0 809 1322 1821 Size: 809 513 499 Fragments arranged by size: 809 513 499 BssSI C'ACGA_G Cuts at: 0 847 979 1821 Size: 847 132 842 Fragments arranged by size: 847 842 132 BstAPI GCAn_nnn'nTGC Cuts at: 0 1071 1278 1481 1821 Size: 1071 207 203 340 Fragments arranged by size: 1071 340 207 203 BstXI CCAn_nnnn'nTGG Cuts at: 0 354 393 1116 1821 Size: 354 39 723 705 Fragments arranged by size: 723 705 354 39 Bsu36I CC'TnA_GG Cuts at: 0 100 1821 Size: 100 1721 BtgI C'CryG_G Cuts at: 0 353 1560 1655 1700 1821 Size: 353 1207 95 45 121 Fragments arranged by size: 1207 353 121 95 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1794 1821 Size: 1794 27 DraIII CAC_nnn'GTG Cuts at: 0 132 1821 Size: 132 1689 EaeI y'GGCC_r Cuts at: 0 960 1448 1753 1821 Size: 960 488 305 68 Fragments arranged by size: 960 488 305 68 EarI CTCTTCn'nnn_ Cuts at: 0 991 1129 1309 1821 Size: 991 138 180 512 Fragments arranged by size: 991 512 180 138 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1255 1273 1821 Size: 1255 18 548 Fragments arranged by size: 1255 548 18 EcoICRI GAG'CTC Cuts at: 0 117 984 1122 1251 1821 Size: 117 867 138 129 570 Fragments arranged by size: 867 570 138 129 117 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 22 259 559 754 768 1123 1138 1243 Size: 22 237 300 195 14 355 15 105 Cuts at: 1243 1261 1441 1705 1744 1750 1821 Size: 18 180 264 39 6 71 Fragments arranged by size: 355 300 264 237 195 180 105 71 39 22 18 15 14 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 79 165 607 1291 1821 Size: 79 86 442 684 530 Fragments arranged by size: 684 530 442 86 79 EcoO109I rG'GnC_Cy Cuts at: 0 104 600 729 1524 1821 Size: 104 496 129 795 297 Fragments arranged by size: 795 496 297 129 104 HaeII r_GCGC'y Cuts at: 0 1278 1332 1406 1821 Size: 1278 54 74 415 Fragments arranged by size: 1278 415 74 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 483 515 619 651 1821 Size: 483 32 104 32 1170 Fragments arranged by size: 1170 483 104 32 32 KasI G'GCGC_C Cuts at: 0 1328 1402 1821 Size: 1328 74 419 Fragments arranged by size: 1328 419 74 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 800 1821 Size: 800 1021 MslI CAynn'nnrTG Cuts at: 0 1066 1821 Size: 1066 755 MspA1I CmG'CkG Cuts at: 0 276 339 585 594 879 1005 1083 1692 Size: 276 63 246 9 285 126 78 609 Cuts at: 1692 1821 Size: 129 Fragments arranged by size: 609 285 276 246 129 126 78 63 9 NaeI GCC'GGC Cuts at: 0 1324 1821 Size: 1324 497 NarI GG'CG_CC Cuts at: 0 1329 1403 1821 Size: 1329 74 418 Fragments arranged by size: 1329 418 74 NcoI C'CATG_G Cuts at: 0 353 1655 1700 1821 Size: 353 1302 45 121 Fragments arranged by size: 1302 353 121 45 NgoMIV G'CCGG_C Cuts at: 0 1322 1821 Size: 1322 499 NspI r_CATG'y Cuts at: 0 411 552 774 1821 Size: 411 141 222 1047 Fragments arranged by size: 1047 411 222 141 PasI CC'CwG_GG Cuts at: 0 267 1821 Size: 267 1554 PciI A'CATG_T Cuts at: 0 770 1821 Size: 770 1051 PfoI T'CCnGG_A Cuts at: 0 1493 1507 1821 Size: 1493 14 314 Fragments arranged by size: 1493 314 14 PpuMI rG'GwC_Cy Cuts at: 0 600 729 1524 1821 Size: 600 129 795 297 Fragments arranged by size: 795 600 297 129 PstI C_TGCA'G Cuts at: 0 380 518 599 1382 1807 1821 Size: 380 138 81 783 425 14 Fragments arranged by size: 783 425 380 138 81 14 PvuII CAG'CTG Cuts at: 0 276 339 594 879 1005 1083 1692 1821 Size: 276 63 255 285 126 78 609 129 Fragments arranged by size: 609 285 276 255 129 126 78 63 SacI G_AGCT'C Cuts at: 0 119 986 1124 1253 1821 Size: 119 867 138 129 568 Fragments arranged by size: 867 568 138 129 119 SapI GCTCTTCn'nnn_ Cuts at: 0 991 1129 1309 1821 Size: 991 138 180 512 Fragments arranged by size: 991 512 180 138 SfcI C'TryA_G Cuts at: 0 319 376 514 570 595 1378 1803 1821 Size: 319 57 138 56 25 783 425 18 Fragments arranged by size: 783 425 319 138 57 56 25 18 SfoI GGC'GCC Cuts at: 0 1330 1404 1821 Size: 1330 74 417 Fragments arranged by size: 1330 417 74 SmaI CCC'GGG Cuts at: 0 865 1821 Size: 865 956 SmlI C'TyrA_G Cuts at: 0 252 1821 Size: 252 1569 SnaBI TAC'GTA Cuts at: 0 510 1821 Size: 510 1311 SphI G_CATG'C Cuts at: 0 552 1821 Size: 552 1269 StyI C'CwwG_G Cuts at: 0 75 228 347 353 1133 1655 1700 1821 Size: 75 153 119 6 780 522 45 121 Fragments arranged by size: 780 522 153 121 119 75 45 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 963 1821 Size: 963 858 TatI w'GTAC_w Cuts at: 0 776 1821 Size: 776 1045 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1194 1226 1327 1359 1821 Size: 1194 32 101 32 462 Fragments arranged by size: 1194 462 101 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1057 1821 Size: 1057 764 XmaI C'CCGG_G Cuts at: 0 863 1821 Size: 863 958 Enzymes that do cut: AarI AcuI AfeI AflIII AlfI AlwNI ApaLI AvaI AvrII BanI BanII BbeI BbvCI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI Bsu36I BtgI BtgZI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PpuMI PstI PvuII SacI SapI SfcI SfoI SmaI SmlI SnaBI SphI StyI TaqII TatI TstI Tth111I XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AleI AloI ApaI ApoI AscI AseI AsiSI BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrGI BssHII BstBI BstEII BstYI BstZ17I BtsI ClaI CspCI DraI DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NdeI NheI NotI NruI NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SpeI SrfI SspI StuI SwaI XbaI XcmI XhoI XmnI ZraI