################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AvrII BamHI BbeI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BstAPI BstBI CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI FseI Hin1I HindIII KasI KpnI KspI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NspI NspV PacI PaeR7I PflMI PinAI PleI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccII 981 CGCG CG^CG Alw44I 1075 GTGCAC G^TGCAC ApaI 1559 GGGCCC GGGCC^C ApaLI 1075 GTGCAC G^TGCAC Asp700I 247 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 391 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 872 TGCGCA TGC^GCA BbrPI 1405 CACGTG CAC^GTG BbsI 298 GAAGAC GAAGAC(2/6) BclI 743 TGATCA T^GATCA BglI 991 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 298 GAAGAC GAAGAC(2/6) BsaAI 1405 CACGTG [CT]AC^GT[GA] BsaBI 646 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1498 GAGACC GGTCTC(1/5) BseRI 52 GAGGAG GAGGAG(10/8) BsgI 18 CTGCAC GTGCAG(16/14) Bst1107I 347 GTATAC GTA^TAC BstEII 1303 GGTAACC G^GT[ACGT]ACC BstPI 1303 GGTAACC G^GT[ACGT]ACC BstUI 981 CGCG CG^CG BstXI 1404 CCACGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 564 AGATCC [GA]^GATC[CT] BstZ17I 347 GTATAC GTA^TAC EcoNI 1308 CCTCGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1303 GGTAACC G^GT[ACGT]ACC EcoRV 586 GATATC GAT^ATC EcoT14I 1299 CCTTGG C^C[AT][AT]GG EcoT22I 844 ATGCAT ATGCA^T FbaI 743 TGATCA T^GATCA FspI 872 TGCGCA TGC^GCA HaeII 985 AGCGCC [GA]GCGC^[CT] HgaI 1420 GACGC GACGC(5/10) HpaI 370 GTTAAC GTT^AAC MamI 646 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 564 AGATCC [GA]^GATC[CT] MvnI 981 CGCG CG^CG NaeI 991 GCCGGC GCC^GGC NgoMIV 991 GCCGGC G^CCGGC NsiI 844 ATGCAT ATGCA^T PflFI 391 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1405 CACGTG CAC^GTG PmlI 1405 CACGTG CAC^GTG PshAI 1500 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1576 CTGCAG CTGCA^G PvuII 1213 CAGCTG CAG^CTG SacI 957 GAGCTC GAGCT^C SalI 194 GTCGAC G^TCGAC SapI 45 GCTCTTC GCTCTTC(1/4) ScaI 317 AGTACT AGT^ACT StyI 1299 CCTTGG C^C[AT][AT]GG Tsp509I 146 AATT ^AATT Tth111I 391 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 700 CCATCACCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 564 AGATCC [GA]^GATC[CT] XmnI 247 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 194 GTCGAC GT^[AC][GT]AC 347 GTATAC AflIII 22 ACACGT A^C[GA][CT]GT 1398 ACACGT AocI 547 CCTGAGG CC^T[ACGT]AGG 1291 CCTGAGG AspHI 957 GAGCTC G[AT]GC[AT]^C 1075 GTGCAC AvaI 155 CCCGGG C^[CT]CG[GA]G 1502 CCCGGG BalI 2 TGGCCA TGG^CCA 308 TGGCCA BfaI 172 CTAG C^TAG 478 CTAG BsiHKAI 957 GAGCTC G[AT]GC[AT]^C 1075 GTGCAC BsoBI 155 CCCGGG C^[CT]CG[GA]G 1502 CCCGGG BsrFI 280 ACCGGC [GA]^CCGG[CT] 991 GCCGGC BsrI 1452 CCAGT ACTGG(1/-1) 1488 CCAGT BssSI 262 CTCGTG CACGAG(-5/-1) 1309 CTCGTG Bsu36I 547 CCTGAGG CC^T[ACGT]AGG 1291 CCTGAGG Cfr10I 280 ACCGGC [GA]^CCGG[CT] 991 GCCGGC DsaI 808 CCGTGG C^C[GA][CT]GG 827 CCACGG EarI 46 CTCTTC CTCTTC(1/4) 1495 GAAGAG Eco81I 547 CCTGAGG CC^T[ACGT]AGG 1291 CCTGAGG HinfI 357 GATTC G^A[ACGT]TC 1347 GAATC Ksp632I 46 CTCTTC CTCTTC(1/4) 1495 GAAGAG MaeI 172 CTAG C^TAG 478 CTAG MluNI 2 TGGCCA TGG^CCA 308 TGGCCA MscI 2 TGGCCA TGG^CCA 308 TGGCCA MseI 371 TTAA T^TAA 1199 TTAA MspA1I 1213 CAGCTG C[AC]G^C[GT]G 1573 CCGCTG PpuMI 404 GGGACCT [GA]G^G[AT]CC[CT] 1169 AGGACCT SmaI 155 CCCGGG CCC^GGG 1502 CCCGGG SmlI 555 CTTGAG C^T[CT][GA]AG 799 CTCAAG StuI 1247 AGGCCT AGG^CCT 1272 AGGCCT TfiI 357 GATTC G^A[AT]TC 1347 GAATC Tru9I 371 TTAA T^TAA 1199 TTAA XmaCI 155 CCCGGG C^CCGGG 1502 CCCGGG XmaI 155 CCCGGG C^CCGGG 1502 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI TspRI TthHB8I