(Linear) (Six-base) MAPSORT of: KIEE0974.seq Check: 4192 from: 1 to: 1581 KIEE0974.orf With 182 enzymes: SgfI * July 15, 2010 18:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 25 77 1581 Size: 25 52 1504 Fragments arranged by size: 1504 52 25 AccI GT'mk_AC Cuts at: 0 195 348 1581 Size: 195 153 1233 Fragments arranged by size: 1233 195 153 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 263 430 749 919 1581 Size: 263 167 319 170 662 Fragments arranged by size: 662 319 263 170 167 AflIII A'CryG_T Cuts at: 0 22 1398 1581 Size: 22 1376 183 Fragments arranged by size: 1376 183 22 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1050 1082 1581 Size: 1050 32 499 Fragments arranged by size: 1050 499 32 AlwNI CAG_nnn'CTG Cuts at: 0 344 360 756 1581 Size: 344 16 396 825 Fragments arranged by size: 825 396 344 16 ApaI G_GGCC'C Cuts at: 0 1563 1581 Size: 1563 18 ApaLI G'TGCA_C Cuts at: 0 1075 1581 Size: 1075 506 AvaI C'yCGr_G Cuts at: 0 155 1502 1581 Size: 155 1347 79 Fragments arranged by size: 1347 155 79 BanI G'GyrC_C Cuts at: 0 448 526 904 1511 1581 Size: 448 78 378 607 70 Fragments arranged by size: 607 448 378 78 70 BanII G_rGCy'C Cuts at: 0 961 1135 1563 1581 Size: 961 174 428 18 Fragments arranged by size: 961 428 174 18 BbsI GAAGACnn'nnnn_ Cuts at: 0 305 1581 Size: 305 1276 BclI T'GATC_A Cuts at: 0 743 1581 Size: 743 838 BfrBI ATG'CAT Cuts at: 0 846 1581 Size: 846 735 BglI GCCn_nnn'nGGC Cuts at: 0 997 1581 Size: 997 584 Bme1580I G_kGCm'C Cuts at: 0 1079 1563 1581 Size: 1079 484 18 Fragments arranged by size: 1079 484 18 BmgBI CAC'GTC Cuts at: 0 1401 1581 Size: 1401 180 BmrI ACTGGGnnnn_n' Cuts at: 0 1446 1581 Size: 1446 135 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 382 704 915 1581 Size: 382 322 211 666 Fragments arranged by size: 666 382 322 211 Bpu10I CC'TnA_GC Cuts at: 0 1554 1581 Size: 1554 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 576 784 1581 Size: 576 208 797 Fragments arranged by size: 797 576 208 BsaI GGTCTCn'nnnn_ Cuts at: 0 1492 1581 Size: 1492 89 BsaAI yAC'GTr Cuts at: 0 1407 1581 Size: 1407 174 BsaBI GATnn'nnATC Cuts at: 0 650 1581 Size: 650 931 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 708 738 1581 Size: 708 30 843 Fragments arranged by size: 843 708 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 67 1581 Size: 67 1514 BseYI C'CCAG_C Cuts at: 0 768 1581 Size: 768 813 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3 1581 Size: 3 1578 BsiHKAI G_wGCw'C Cuts at: 0 961 1079 1581 Size: 961 118 502 Fragments arranged by size: 961 502 118 Bsp1286I G_dGCh'C Cuts at: 0 961 1079 1135 1563 1581 Size: 961 118 56 428 18 Fragments arranged by size: 961 428 118 56 18 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 25 77 431 1581 Size: 25 52 354 1150 Fragments arranged by size: 1150 354 52 25 BsrFI r'CCGG_y Cuts at: 0 280 991 1581 Size: 280 711 590 Fragments arranged by size: 711 590 280 BssSI C'ACGA_G Cuts at: 0 262 1309 1581 Size: 262 1047 272 Fragments arranged by size: 1047 272 262 BstEII G'GTnAC_C Cuts at: 0 1303 1581 Size: 1303 278 BstXI CCAn_nnnn'nTGG Cuts at: 0 1411 1581 Size: 1411 170 BstYI r'GATC_y Cuts at: 0 564 1581 Size: 564 1017 BstZ17I GTA'TAC Cuts at: 0 349 1581 Size: 349 1232 Bsu36I CC'TnA_GG Cuts at: 0 548 1292 1581 Size: 548 744 289 Fragments arranged by size: 744 548 289 BtgI C'CryG_G Cuts at: 0 808 827 1581 Size: 808 19 754 Fragments arranged by size: 808 754 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1416 1581 Size: 1416 165 BtsI GCAGTG_nn' Cuts at: 0 1548 1581 Size: 1548 33 EaeI y'GGCC_r Cuts at: 0 2 308 998 1581 Size: 2 306 690 583 Fragments arranged by size: 690 583 306 2 EarI CTCTTCn'nnn_ Cuts at: 0 52 1490 1581 Size: 52 1438 91 Fragments arranged by size: 1438 91 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 951 1432 1581 Size: 951 481 149 Fragments arranged by size: 951 481 149 EcoICRI GAG'CTC Cuts at: 0 959 1581 Size: 959 622 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 263 382 430 704 749 915 919 1581 Size: 263 119 48 274 45 166 4 662 Fragments arranged by size: 662 274 263 166 119 48 45 4 EcoNI CCTnn'n_nnAGG Cuts at: 0 1312 1581 Size: 1312 269 EcoO109I rG'GnC_Cy Cuts at: 0 405 1170 1296 1559 1560 1581 Size: 405 765 126 263 1 21 Fragments arranged by size: 765 405 263 126 21 1 EcoRV GAT'ATC Cuts at: 0 588 1581 Size: 588 993 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1356 1581 Size: 1356 225 FspI TGC'GCA Cuts at: 0 874 1581 Size: 874 707 HaeII r_GCGC'y Cuts at: 0 989 1581 Size: 989 592 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 549 581 685 717 834 866 1251 1283 Size: 549 32 104 32 117 32 385 32 Cuts at: 1283 1581 Size: 298 Fragments arranged by size: 549 385 298 117 104 32 32 32 32 HincII GTy'rAC Cuts at: 0 196 372 820 1581 Size: 196 176 448 761 Fragments arranged by size: 761 448 196 176 HpaI GTT'AAC Cuts at: 0 372 1581 Size: 372 1209 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1016 1581 Size: 1016 565 MscI TGG'CCA Cuts at: 0 4 310 1581 Size: 4 306 1271 Fragments arranged by size: 1271 306 4 MslI CAynn'nnrTG Cuts at: 0 386 708 919 1581 Size: 386 322 211 662 Fragments arranged by size: 662 386 322 211 MspA1I CmG'CkG Cuts at: 0 1215 1575 1581 Size: 1215 360 6 Fragments arranged by size: 1215 360 6 NaeI GCC'GGC Cuts at: 0 993 1581 Size: 993 588 NgoMIV G'CCGG_C Cuts at: 0 991 1581 Size: 991 590 NsiI A_TGCA'T Cuts at: 0 848 1581 Size: 848 733 PfoI T'CCnGG_A Cuts at: 0 931 1581 Size: 931 650 PmlI CAC'GTG Cuts at: 0 1407 1581 Size: 1407 174 PpuMI rG'GwC_Cy Cuts at: 0 405 1170 1581 Size: 405 765 411 Fragments arranged by size: 765 411 405 PshAI GACnn'nnGTC Cuts at: 0 1504 1581 Size: 1504 77 PspOMI G'GGCC_C Cuts at: 0 1559 1581 Size: 1559 22 PstI C_TGCA'G Cuts at: 0 1580 1581 Size: 1580 1 PvuII CAG'CTG Cuts at: 0 1215 1581 Size: 1215 366 SacI G_AGCT'C Cuts at: 0 961 1581 Size: 961 620 SalI G'TCGA_C Cuts at: 0 194 1581 Size: 194 1387 SapI GCTCTTCn'nnn_ Cuts at: 0 52 1581 Size: 52 1529 ScaI AGT'ACT Cuts at: 0 319 1581 Size: 319 1262 SfcI C'TryA_G Cuts at: 0 321 1115 1576 1581 Size: 321 794 461 5 Fragments arranged by size: 794 461 321 5 SmaI CCC'GGG Cuts at: 0 157 1504 1581 Size: 157 1347 77 Fragments arranged by size: 1347 157 77 SmlI C'TyrA_G Cuts at: 0 555 799 1581 Size: 555 244 782 Fragments arranged by size: 782 555 244 StuI AGG'CCT Cuts at: 0 1249 1274 1581 Size: 1249 25 307 Fragments arranged by size: 1249 307 25 StyI C'CwwG_G Cuts at: 0 1299 1581 Size: 1299 282 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1465 1581 Size: 1465 116 TatI w'GTAC_w Cuts at: 0 317 489 1454 1581 Size: 317 172 965 127 Fragments arranged by size: 965 317 172 127 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 566 598 708 740 1411 1443 1513 1545 Size: 566 32 110 32 671 32 70 32 Cuts at: 1545 1581 Size: 36 Fragments arranged by size: 671 566 110 70 36 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 394 1581 Size: 394 1187 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 707 1581 Size: 707 874 XmaI C'CCGG_G Cuts at: 0 155 1502 1581 Size: 155 1347 79 Fragments arranged by size: 1347 155 79 XmnI GAAnn'nnTTC Cuts at: 0 251 1581 Size: 251 1330 Enzymes that do cut: AarI AccI AcuI AflIII AloI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BclI BfrBI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BssSI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII HpaI MmeI MscI MslI MspA1I NaeI NgoMIV NsiI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI SalI SapI ScaI SfcI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BglII BlpI BmtI BplI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI ClaI CspCI DraI DraIII DrdI EagI EcoRI FseI FspAI HindIII KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI NspI PacI PasI PciI PflMI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacII SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI ZraI