################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## Acc65I AccI AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AvrII BcgI BciVI BclI BfrI BglII BlnI BsaBI BsiWI BspDI BspHI BspLU11I BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI MamI MfeI MluI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I RcaI SalI SbfI ScaI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tru9I XbaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 2388 GACGTC GACGT^C AccIII 734 TCCGGA T^CCGGA AhdI 709 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 709 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2229 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1295 TGCGCA TGC^GCA BalI 2153 TGGCCA TGG^CCA BamHI 2278 GGATCC G^GATCC BfaI 374 CTAG C^TAG BseAI 734 TCCGGA T^CCGGA Bsp1407I 671 TGTACA T^GTACA BspEI 734 TCCGGA T^CCGGA BsrDI 2372 GCAATG GCAATG(2/0) BsrGI 671 TGTACA T^GTACA BssSI 1948 CACGAG CACGAG(-5/-1) BstXI 1957 CCACTGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 2278 GGATCC [GA]^GATC[CT] CpoI 2407 CGGACCG CG^G[AT]CCG Eam1105I 709 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 1295 TGCGCA TGC^GCA KspI 1639 CCGCGG CCGC^GG MaeI 374 CTAG C^TAG MflI 2278 GGATCC [GA]^GATC[CT] MluNI 2153 TGGCCA TGG^CCA MroI 734 TCCGGA T^CCGGA MscI 2153 TGGCCA TGG^CCA NaeI 452 GCCGGC GCC^GGC NcoI 1334 CCATGG C^CATGG NgoMIV 452 GCCGGC G^CCGGC PaeR7I 2549 CTCGAG C^TCGAG PflFI 2229 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 1425 CGATCG CGAT^CG RsrII 2407 CGGACCG CG^G[AT]CCG SacI 1396 GAGCTC GAGCT^C SacII 1639 CCGCGG CCGC^GG SexAI 1011 ACCAGGT A^CC[AT]GGT SmaI 563 CCCGGG CCC^GGG SspBI 671 TGTACA T^GTACA StuI 281 AGGCCT AGG^CCT TfiI 2417 GAATC G^A[AT]TC Tth111I 2229 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2549 CTCGAG C^TCGAG XhoII 2278 GGATCC [GA]^GATC[CT] XmaCI 563 CCCGGG C^CCGGG XmaI 563 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccII 1640 CGCG CG^CG 2146 CGCG AcsI 1790 AAATTC [GA]^AATT[CT] 2668 GAATTT ApoI 1790 AAATTC [GA]^AATT[CT] 2668 GAATTT AspHI 1396 GAGCTC G[AT]GC[AT]^C 2255 GTGCTC BsaI 2635 GAGACC GGTCTC(1/5) 2682 GAGACC BsiHKAI 1396 GAGCTC G[AT]GC[AT]^C 2255 GTGCTC BsmI 1342 GAATGC GAATGC(1/-1) 1410 GCATTC BstAPI 1221 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1998 GCATGTGCTGC BstUI 1640 CGCG CG^CG 2146 CGCG EaeI 2153 TGGCCA [CT]^GGCC[GA] 2379 TGGCCG EcoNI 144 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1461 CCTCATCGAGG HincII 655 GTCAAC GT[CT]^[GA]AC 1804 GTCAAC HindII 655 GTCAAC GT[CT]^[GA]AC 1804 GTCAAC MvnI 1640 CGCG CG^CG 2146 CGCG SfiI 273 GGCCAAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2452 GGCCCCGAGGGCC SphI 237 GCATGC GCATG^C 1298 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI ApaI Asp700I AvaI AvaII BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XmnI