(Circular) (Six-base) MAPSORT of: pFN21AE6728.seq Check: 8855 from: 1 to: 7630 pFN21AE6728 7630 bp With 182 enzymes: SgfI * October 20, 2017 17:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5440 278 Size: 53 83 186 936 3904 2468 Fragments arranged by size: 3904 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4973 1937 Size: 3036 4594 Acc65I G'GTAC_C Cuts at: 4951 4951 Size: 7630 AclI AA'CG_TT Cuts at: 1563 5757 6130 1563 Size: 4194 373 3063 Fragments arranged by size: 4194 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2021 3156 3252 4796 5693 7077 7345 1632 Size: 389 1135 96 1544 897 1384 268 1917 Fragments arranged by size: 1917 1544 1384 1135 897 389 268 96 AflII C'TTAA_G Cuts at: 820 1017 3538 820 Size: 197 2521 4912 Fragments arranged by size: 4912 2521 197 AflIII A'CryG_T Cuts at: 1203 2230 3982 6529 7506 1203 Size: 1027 1752 2547 977 1327 Fragments arranged by size: 2547 1752 1327 1027 977 AgeI A'CCGG_T Cuts at: 6461 6461 Size: 7630 AhdI GACnn_n'nnGTC Cuts at: 6359 6359 Size: 7630 AleI CACnn'nnGTG Cuts at: 1166 3323 1166 Size: 2157 5473 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7630 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3668 3700 4845 4877 3668 Size: 32 1145 32 6421 Fragments arranged by size: 6421 1145 32 32 AlwNI CAG_nnn'CTG Cuts at: 2752 3706 5373 6945 2752 Size: 954 1667 1572 3437 Fragments arranged by size: 3437 1667 1572 954 ApaLI G'TGCA_C Cuts at: 5687 6843 5687 Size: 1156 6474 ApoI r'AATT_y Cuts at: 1493 2532 2894 3016 3524 3644 4163 4451 4601 Size: 1039 362 122 508 120 519 288 150 Cuts at: 4601 4939 5165 5317 7426 1493 Size: 338 226 152 2109 1697 Fragments arranged by size: 2109 1697 1039 519 508 362 338 288 226 152 150 122 120 AseI AT'TA_AT Cuts at: 160 1032 6184 160 Size: 872 5152 1606 Fragments arranged by size: 5152 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7630 AvaI C'yCGr_G Cuts at: 1870 1943 4955 1870 Size: 73 3012 4545 Fragments arranged by size: 4545 3012 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2058 2091 2058 Size: 33 7597 BamHI G'GATC_C Cuts at: 4960 5327 4960 Size: 367 7263 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4951 6406 618 Size: 325 217 627 3164 1455 1842 Fragments arranged by size: 3164 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2286 3396 4949 1391 Size: 895 1110 1553 4072 Fragments arranged by size: 4072 1553 1110 895 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3168 928 Size: 971 1269 5390 Fragments arranged by size: 5390 1269 971 BbvCI CC'TCA_GC Cuts at: 2088 2088 Size: 7630 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5821 5855 1903 Size: 34 3884 34 3678 Fragments arranged by size: 3884 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2059 4365 4879 5522 6732 2059 Size: 2306 514 643 1210 2957 Fragments arranged by size: 2957 2306 1210 643 514 BclI T'GATC_A Cuts at: 1548 3415 1548 Size: 1867 5763 BfrBI ATG'CAT Cuts at: 3739 3739 Size: 7630 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6241 136 Size: 107 122 71 631 605 4569 1525 Fragments arranged by size: 4569 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7625 7625 Size: 7630 BlpI GC'TnA_GC Cuts at: 5047 5047 Size: 7630 Bme1580I G_kGCm'C Cuts at: 1790 5691 6847 1790 Size: 3901 1156 2573 Fragments arranged by size: 3901 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2231 1318 Size: 913 6717 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3938 5361 6319 Size: 416 53 83 399 24 2521 1423 958 Cuts at: 6319 442 Size: 1753 Fragments arranged by size: 2521 1753 1423 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7630 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2245 6290 1239 Size: 1006 4045 2579 Fragments arranged by size: 4045 2579 1006 Bpu10I CC'TnA_GC Cuts at: 2088 2088 Size: 7630 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4235 4724 4811 5755 6620 6918 4235 Size: 489 87 944 865 298 4947 Fragments arranged by size: 4947 944 865 489 298 87 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6293 882 Size: 617 4794 2219 Fragments arranged by size: 4794 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4374 5421 493 Size: 711 3170 1047 2702 Fragments arranged by size: 3170 2702 1047 711 BsaBI GATnn'nnATC Cuts at: 2853 3414 7624 2853 Size: 561 4210 2859 Fragments arranged by size: 4210 2859 561 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5437 5819 6454 275 Size: 53 83 186 936 3904 382 635 1451 Fragments arranged by size: 3904 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6063 6461 6735 6882 7293 6063 Size: 398 274 147 411 6400 Fragments arranged by size: 6400 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3262 3292 1178 Size: 30 165 30 1859 30 5516 Fragments arranged by size: 5516 1859 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2270 2273 2862 3210 1186 Size: 919 165 3 589 348 5606 Fragments arranged by size: 5606 919 589 348 165 3 BseYI C'CCAG_C Cuts at: 4524 4902 6833 7329 4524 Size: 378 1931 496 4825 Fragments arranged by size: 4825 1931 496 378 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2298 1163 Size: 573 562 6495 Fragments arranged by size: 6495 573 562 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5080 5841 6869 1540 Size: 275 186 3079 761 1028 2301 Fragments arranged by size: 3079 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2340 3396 4251 5691 5776 6847 2340 Size: 1056 855 1440 85 1071 3123 Fragments arranged by size: 3123 1440 1071 1056 855 85 BsmI GAATG_Cn' Cuts at: 2762 3068 3343 5141 5234 2762 Size: 306 275 1798 93 5158 Fragments arranged by size: 5158 1798 306 275 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2221 2221 Size: 7630 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2286 2340 3396 4251 4949 5691 5776 Size: 399 496 54 1056 855 698 742 85 Cuts at: 5776 6847 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1056 855 742 698 496 399 85 54 BspEI T'CCGG_A Cuts at: 7293 7293 Size: 7630 BspHI T'CATG_A Cuts at: 4647 5519 7249 4647 Size: 872 1730 5028 Fragments arranged by size: 5028 1730 872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4985 844 Size: 4141 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5517 1394 Size: 429 3694 3507 Fragments arranged by size: 3694 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2340 2608 3476 6125 6299 65 Size: 1183 1092 268 868 2649 174 1396 Fragments arranged by size: 2649 1396 1183 1092 868 268 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6274 6461 1700 Size: 110 4464 187 2869 Fragments arranged by size: 4464 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7630 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7630 BssSI C'ACGA_G Cuts at: 5690 6702 5690 Size: 1012 6618 BstAPI GCAn_nnn'nTGC Cuts at: 794 2243 2351 3745 4843 794 Size: 1449 108 1394 1098 3581 Fragments arranged by size: 3581 1449 1394 1098 108 BstBI TT'CG_AA Cuts at: 6434 6434 Size: 7630 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7630 BstXI CCAn_nnnn'nTGG Cuts at: 2476 3325 2476 Size: 849 6781 BstYI r'GATC_y Cuts at: 1972 2008 4657 4960 5327 5712 5729 7181 7267 Size: 36 2649 303 367 385 17 1452 86 Cuts at: 7267 7279 7625 1972 Size: 12 346 1977 Fragments arranged by size: 2649 1977 1452 385 367 346 303 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6139 Fragments arranged by size: 6139 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6891 Fragments arranged by size: 6891 683 56 BtsI GCAGTG_nn' Cuts at: 2111 2340 2636 4799 5146 5940 5960 2111 Size: 229 296 2163 347 794 20 3781 Fragments arranged by size: 3781 2163 794 347 296 229 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7630 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4360 4395 529 Size: 35 3796 35 3764 Fragments arranged by size: 3796 3764 35 35 DraI TTT'AAA Cuts at: 2203 3427 4215 4934 2203 Size: 1224 788 719 4899 Fragments arranged by size: 4899 1224 788 719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6637 809 Size: 507 5321 1802 Fragments arranged by size: 5321 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5077 5966 7595 Size: 54 1425 50 275 18 3247 889 1629 Cuts at: 7595 8 Size: 43 Fragments arranged by size: 3247 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5077 1537 Size: 275 3265 4090 Fragments arranged by size: 4090 3265 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4572 5560 1353 Size: 3219 988 3423 Fragments arranged by size: 3423 3219 988 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6217 6591 6737 387 Size: 1064 138 4628 374 146 1280 Fragments arranged by size: 4628 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3394 3394 Size: 7630 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2021 2245 3156 3252 4796 5693 6290 Size: 393 389 224 911 96 1544 897 597 Cuts at: 6290 7077 7345 1239 Size: 787 268 1524 Fragments arranged by size: 1544 1524 911 897 787 597 393 389 268 224 96 EcoNI CCTnn'n_nnAGG Cuts at: 4665 4665 Size: 7630 EcoO109I rG'GnC_Cy Cuts at: 3314 4780 3314 Size: 1466 6164 EcoRI G'AATT_C Cuts at: 4939 5317 4939 Size: 378 7252 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3553 3553 Size: 7630 FspI TGC'GCA Cuts at: 6136 6136 Size: 7630 HaeII r_GCGC'y Cuts at: 1678 6777 1678 Size: 5099 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2308 2340 2940 2972 3262 Size: 32 135 32 736 32 600 32 290 Cuts at: 3262 3294 4642 4674 4684 4716 6271 6303 6345 Size: 32 1348 32 10 32 1555 32 42 Cuts at: 6345 6377 1373 Size: 32 2626 Fragments arranged by size: 2626 1555 1348 736 600 290 135 42 32 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 2046 2667 3627 3778 4974 5220 Size: 988 281 108 621 960 151 1196 246 Cuts at: 5220 669 Size: 3079 Fragments arranged by size: 3079 1196 988 960 621 281 246 151 108 HindIII A'AGCT_T Cuts at: 748 748 Size: 7630 HpaI GTT'AAC Cuts at: 2046 2667 3627 5220 2046 Size: 621 960 1593 4456 Fragments arranged by size: 4456 1593 960 621 KpnI G_GTAC'C Cuts at: 4955 4955 Size: 7630 MfeI C'AATT_G Cuts at: 5229 5229 Size: 7630 MluI A'CGCG_T Cuts at: 7506 7506 Size: 7630 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3355 6744 6928 1305 Size: 2050 3389 184 2007 Fragments arranged by size: 3389 2050 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5808 Fragments arranged by size: 5808 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3323 3734 5588 5947 6106 518 Size: 648 2157 411 1854 359 159 2042 Fragments arranged by size: 2157 2042 1854 648 411 359 159 MspA1I CmG'CkG Cuts at: 1606 2087 5046 5723 6871 7116 1606 Size: 481 2959 677 1148 245 2120 Fragments arranged by size: 2959 2120 1148 677 481 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6139 Fragments arranged by size: 6139 939 552 NdeI CA'TA_TG Cuts at: 387 3796 387 Size: 3409 4221 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7630 NotI GC'GGCC_GC Cuts at: 5077 5077 Size: 7630 NsiI A_TGCA'T Cuts at: 3741 3741 Size: 7630 NspI r_CATG'y Cuts at: 1132 1229 3743 3752 3784 3986 4988 6533 1132 Size: 97 2514 9 32 202 1002 1545 2229 Fragments arranged by size: 2514 2229 1545 1002 202 97 32 9 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7630 PciI A'CATG_T Cuts at: 3982 6529 3982 Size: 2547 5083 PflMI CCAn_nnn'nTGG Cuts at: 1108 2164 2376 4477 1108 Size: 1056 212 2101 4261 Fragments arranged by size: 4261 2101 1056 212 PfoI T'CCnGG_A Cuts at: 2418 2418 Size: 7630 PmeI GTTT'AAAC Cuts at: 4934 4934 Size: 7630 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5699 5731 7212 7244 5699 Size: 32 1481 32 6085 Fragments arranged by size: 6085 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3314 4780 3314 Size: 1466 6164 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7630 PsiI TTA'TAA Cuts at: 2538 3003 4616 4677 5200 2538 Size: 465 1613 61 523 4968 Fragments arranged by size: 4968 1613 523 465 61 PstI C_TGCA'G Cuts at: 830 3711 4806 4982 830 Size: 2881 1095 176 3478 Fragments arranged by size: 3478 2881 1095 176 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7630 PvuII CAG'CTG Cuts at: 2087 2087 Size: 7630 SacI G_AGCT'C Cuts at: 3396 3396 Size: 7630 SalI G'TCGA_C Cuts at: 1936 4972 1936 Size: 3036 4594 SbfI CC_TGCA'GG Cuts at: 4982 4982 Size: 7630 ScaI AGT'ACT Cuts at: 5878 7590 5878 Size: 1712 5918 SfcI C'TryA_G Cuts at: 826 1045 3707 4227 4359 4802 4907 4978 6113 Size: 219 2662 520 132 443 105 71 1135 Cuts at: 6113 6794 6985 826 Size: 681 191 1471 Fragments arranged by size: 2662 1471 1135 681 520 443 219 191 132 105 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7630 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7630 SmaI CCC'GGG Cuts at: 1872 4957 1872 Size: 3085 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3538 4250 4703 4826 5734 6635 Size: 197 926 1595 712 453 123 908 901 Cuts at: 6635 6897 820 Size: 262 1553 Fragments arranged by size: 1595 1553 926 908 901 712 453 262 197 123 SnaBI TAC'GTA Cuts at: 493 5421 493 Size: 4928 2702 SpeI A'CTAG_T Cuts at: 152 4412 152 Size: 4260 3370 SphI G_CATG'C Cuts at: 1132 4988 1132 Size: 3856 3774 SspI AAT'ATT Cuts at: 5 52 2146 2730 3818 5554 5 Size: 47 2094 584 1088 1736 2081 Fragments arranged by size: 2094 2081 1736 1088 584 47 StuI AGG'CCT Cuts at: 1517 4635 1517 Size: 3118 4512 StyI C'CwwG_G Cuts at: 513 1065 2004 5069 513 Size: 552 939 3065 3074 Fragments arranged by size: 3074 3065 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3494 4753 4916 5657 5674 5827 6012 Size: 1255 1914 1259 163 741 17 153 185 Cuts at: 6012 6457 325 Size: 445 1498 Fragments arranged by size: 1914 1498 1259 1255 741 445 185 163 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2191 3283 3959 Size: 274 80 33 51 1443 214 1092 676 Cuts at: 3959 5876 7588 96 Size: 1917 1712 138 Fragments arranged by size: 1917 1712 1443 1092 676 274 214 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3826 3858 7590 7622 3826 Size: 32 3732 32 3834 Fragments arranged by size: 3834 3732 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7630 XbaI T'CTAG_A Cuts at: 3289 4966 3289 Size: 1677 5953 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2161 2161 Size: 7630 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7630 XmaI C'CCGG_G Cuts at: 1870 4955 1870 Size: 3085 4545 XmnI GAAnn'nnTTC Cuts at: 2147 2388 4543 5759 2147 Size: 241 2155 1216 4018 Fragments arranged by size: 4018 2155 1216 241 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5438 276 Size: 53 83 186 936 3904 2468 Fragments arranged by size: 3904 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI