Restriction map for
pFN21AE1434
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccI AccII AcyI
AflII AflIII AgeI AhdI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BsaAI BsaBI BsaHI
BsaI BseRI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoO65I FbaI FseI FspI
HgaI Hin1I HindIII KasI KpnI
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 941 TCCGGA T^CCGGA
AclI 930 AACGTT AA^CGTT
AfaI 860 GTAC GT^AC
AocI 140 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2240 AGCGCT AGC^GCT
AvrII 414 CCTAGG C^CTAGG
BglII 552 AGATCT A^GATCT
BlnI 414 CCTAGG C^CTAGG
BlpI 2305 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2305 GCTCAGC GC^T[ACGT]AGC
BseAI 941 TCCGGA T^CCGGA
BsmAI 1219 GAGAC GTCTC(1/5)
BsmFI 2030 GGGAC GGGAC(10/14)
BspEI 941 TCCGGA T^CCGGA
BspMI 1719 GCAGGT ACCTGC(4/8)
BsrBI 1945 GAGCGG CCGCTC(-3/-3)
BsrDI 95 GCAATG GCAATG(2/0)
Bsu36I 140 CCTCAGG CC^T[ACGT]AGG
CelII 2305 GCTCAGC GC^T[ACGT]AGC
CfoI 2241 GCGC GCG^C
DraII 1817 GGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 2124 GAAGAG CTCTTC(1/4)
Eco47III 2240 AGCGCT AGC^GCT
Eco81I 140 CCTCAGG CC^T[ACGT]AGG
EcoNI 48 CCTCTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1817 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1618 GAATTC G^AATTC
EcoRV 1255 GATATC GAT^ATC
EcoT22I 1925 ATGCAT ATGCA^T
HaeII 2240 AGCGCT [GA]GCGC^[CT]
HhaI 2241 GCGC GCG^C
HinP1I 2241 GCGC G^CGC
HincII 933 GTTAAC GT[CT]^[GA]AC
HindII 933 GTTAAC GT[CT]^[GA]AC
HpaI 933 GTTAAC GTT^AAC
Ksp632I 2124 GAAGAG CTCTTC(1/4)
MroI 941 TCCGGA T^CCGGA
MslI 1705 CATGTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 2308 CAGCGG C[AC]G^C[GT]G
NdeI 616 CATATG CA^TATG
NsiI 1925 ATGCAT ATGCA^T
NspI 1778 GCATGT [GA]CATG^[CT]
PpuMI 1817 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 930 AACGTT AA^CGTT
RsaI 860 GTAC GT^AC
SpeI 1503 ACTAGT A^CTAGT
SspI 1316 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspHI 984 GAGCAC G[AT]GC[AT]^C
1787 GTGCTC
AvaII 1818 GGTCC G^G[AT]CC
1977 GGTCC
BanII 145 GGGCTC G[GA]GC[CT]^C
2212 GAGCCC
BfaI 415 CTAG C^TAG
1504 CTAG
BsaWI 941 TCCGGA [AT]^CCGG[AT]
1213 ACCGGA
BsiHKAI 984 GAGCAC G[AT]GC[AT]^C
1787 GTGCTC
BsmI 1524 GAATGC GAATGC(1/-1)
1361 GCATTC
BstXI 1367 CCAAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1704 CCATGTGCGTGG
DraI 126 TTTAAA TTT^AAA
1074 TTTAAA
HapII 942 CCGG C^CGG
1214 CCGG
HpaII 942 CCGG C^CGG
1214 CCGG
MaeI 415 CTAG C^TAG
1504 CTAG
MspI 942 CCGG C^CGG
1214 CCGG
NcoI 1836 CCATGG C^CATGG
2294 CCATGG
PflMI 234 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1980 CCAGGAGCTGG
SfcI 214 CTACAG C^T[GA][CT]AG
556 CTACAG
SmlI 526 CTTGAG C^T[CT][GA]AG
535 CTTGAG
Van91I 234 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1980 CCAGGAGCTGG
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI BalI BbsI BbvI BmyI
BpmI BpuAI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII