(Circular) (Six-base) MAPSORT of: pFN21AE1500.seq Check: 1809 from: 1 to: 7006 pFN21AE1500 7006 bp With 182 enzymes: SgfI * August 26, 2010 19:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4816 278 Size: 53 83 186 936 3280 2468 Fragments arranged by size: 3280 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4349 1937 Size: 2412 4594 Acc65I G'GTAC_C Cuts at: 4327 4327 Size: 7006 AclI AA'CG_TT Cuts at: 1563 3370 5133 5506 1563 Size: 1807 1763 373 3063 Fragments arranged by size: 3063 1807 1763 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 3428 5069 6453 6721 1632 Size: 407 1389 1641 1384 268 1917 Fragments arranged by size: 1917 1641 1389 1384 407 268 AflII C'TTAA_G Cuts at: 820 1017 2680 820 Size: 197 1663 5146 Fragments arranged by size: 5146 1663 197 AflIII A'CryG_T Cuts at: 1203 2950 4102 5905 6882 1203 Size: 1747 1152 1803 977 1327 Fragments arranged by size: 1803 1747 1327 1152 977 AgeI A'CCGG_T Cuts at: 5837 5837 Size: 7006 AhdI GACnn_n'nnGTC Cuts at: 5735 5735 Size: 7006 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7006 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3209 3225 3351 1184 Size: 2025 16 126 4839 Fragments arranged by size: 4839 2025 126 16 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2790 2822 3779 3811 2790 Size: 32 957 32 5985 Fragments arranged by size: 5985 957 32 32 AlwNI CAG_nnn'CTG Cuts at: 2185 3544 4749 6321 2185 Size: 1359 1205 1572 2870 Fragments arranged by size: 2870 1572 1359 1205 ApaI G_GGCC'C Cuts at: 3728 3728 Size: 7006 ApaLI G'TGCA_C Cuts at: 5063 6219 5063 Size: 1156 5850 ApoI r'AATT_y Cuts at: 1493 2318 2992 4115 4315 4541 4693 6802 1493 Size: 825 674 1123 200 226 152 2109 1697 Fragments arranged by size: 2109 1697 1123 825 674 226 200 152 AseI AT'TA_AT Cuts at: 160 1032 5560 160 Size: 872 4528 1606 Fragments arranged by size: 4528 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7006 AvaI C'yCGr_G Cuts at: 1870 1943 3066 3772 4331 1870 Size: 73 1123 706 559 4545 Fragments arranged by size: 4545 1123 706 559 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2195 2228 4286 4319 2195 Size: 33 2058 33 4882 Fragments arranged by size: 4882 2058 33 33 BamHI G'GATC_C Cuts at: 2076 4336 4703 2076 Size: 2260 367 4379 Fragments arranged by size: 4379 2260 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4036 4327 5782 618 Size: 325 217 627 2249 291 1455 1842 Fragments arranged by size: 2249 1842 1455 627 325 291 217 BanII G_rGCy'C Cuts at: 1391 2808 3165 3728 3945 3993 4325 1391 Size: 1417 357 563 217 48 332 4072 Fragments arranged by size: 4072 1417 563 357 332 217 48 BbeI G_GCGC'C Cuts at: 4040 4040 Size: 7006 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2880 3956 928 Size: 971 981 1076 3978 Fragments arranged by size: 3978 1076 981 971 BbvCI CC'TCA_GC Cuts at: 3713 3713 Size: 7006 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3763 3797 5197 5231 1903 Size: 34 1826 34 1400 34 3678 Fragments arranged by size: 3678 1826 1400 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2995 4287 4898 6108 2995 Size: 1292 611 1210 3893 Fragments arranged by size: 3893 1292 1210 611 BclI T'GATC_A Cuts at: 1548 1548 Size: 7006 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2363 5617 136 Size: 107 122 71 631 605 691 3254 1525 Fragments arranged by size: 3254 1525 691 631 605 122 107 71 BglII A'GATC_T Cuts at: 2167 7001 2167 Size: 4834 2172 BlpI GC'TnA_GC Cuts at: 2985 4423 2985 Size: 1438 5568 Bme1580I G_kGCm'C Cuts at: 1790 3728 5067 6223 1790 Size: 1938 1339 1156 2573 Fragments arranged by size: 2573 1938 1339 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7006 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2452 4737 5695 Size: 416 53 83 399 24 1035 2285 958 Cuts at: 5695 442 Size: 1753 Fragments arranged by size: 2285 1753 1035 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7006 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2143 3080 3101 3236 3290 3344 3482 3554 Size: 904 937 21 135 54 54 138 72 Cuts at: 3554 3575 3617 3671 3917 3959 4238 5666 1239 Size: 21 42 54 246 42 279 1428 2579 Fragments arranged by size: 2579 1428 937 904 279 246 138 135 72 54 54 54 42 42 21 21 Bpu10I CC'TnA_GC Cuts at: 3042 3713 3042 Size: 671 6335 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2847 3438 3653 4190 5131 5996 6294 2847 Size: 591 215 537 941 865 298 3559 Fragments arranged by size: 3559 941 865 591 537 298 215 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5669 882 Size: 617 4170 2219 Fragments arranged by size: 4170 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2953 3766 4797 493 Size: 711 1749 813 1031 2702 Fragments arranged by size: 2702 1749 1031 813 711 BsaBI GATnn'nnATC Cuts at: 7000 7000 Size: 7006 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4037 4813 5195 5830 Size: 53 83 186 936 2504 776 382 635 Cuts at: 5830 275 Size: 1451 Fragments arranged by size: 2504 1451 936 776 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3945 5439 5837 6111 6258 6669 3945 Size: 1494 398 274 147 411 4282 Fragments arranged by size: 4282 1494 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2790 2820 3209 3239 1178 Size: 30 165 30 1387 30 389 30 4945 Fragments arranged by size: 4945 1387 389 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2660 3119 3479 3692 4205 4208 1186 Size: 1474 459 360 213 513 3 3984 Fragments arranged by size: 3984 1474 513 459 360 213 3 BseYI C'CCAG_C Cuts at: 2178 6209 6705 2178 Size: 4031 496 2479 Fragments arranged by size: 4031 2479 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2784 3215 1163 Size: 573 1048 431 4954 Fragments arranged by size: 4954 1048 573 431 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4274 4456 5217 6245 1540 Size: 275 186 2273 182 761 1028 2301 Fragments arranged by size: 2301 2273 1028 761 275 186 182 BsiHKAI G_wGCw'C Cuts at: 2808 3165 3945 3993 5067 5152 6223 2808 Size: 357 780 48 1074 85 1071 3591 Fragments arranged by size: 3591 1074 1071 780 357 85 48 BsmI GAATG_Cn' Cuts at: 3282 3356 4517 4610 3282 Size: 74 1161 93 5678 Fragments arranged by size: 5678 1161 93 74 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2808 3165 3728 3945 3993 4325 5067 Size: 399 1018 357 563 217 48 332 742 Cuts at: 5067 5152 6223 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1018 742 563 399 357 332 217 85 48 BspEI T'CCGG_A Cuts at: 3945 6669 3945 Size: 2724 4282 BspHI T'CATG_A Cuts at: 4895 6625 4895 Size: 1730 5276 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3717 4361 844 Size: 2873 644 3489 Fragments arranged by size: 3489 2873 644 BsrBI CCG'CTC Cuts at: 1394 1823 4893 1394 Size: 429 3070 3507 Fragments arranged by size: 3507 3070 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3728 5501 5675 65 Size: 1183 2480 1773 174 1396 Fragments arranged by size: 2480 1773 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5650 5837 1700 Size: 110 3840 187 2869 Fragments arranged by size: 3840 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7006 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7006 BssSI C'ACGA_G Cuts at: 5066 6078 5066 Size: 1012 5994 BstAPI GCAn_nnn'nTGC Cuts at: 794 3091 3934 794 Size: 2297 843 3866 Fragments arranged by size: 3866 2297 843 BstBI TT'CG_AA Cuts at: 3996 5810 3996 Size: 1814 5192 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7006 BstYI r'GATC_y Cuts at: 1972 2076 2167 3847 4336 4703 5088 5105 6557 Size: 104 91 1680 489 367 385 17 1452 Cuts at: 6557 6643 6655 7001 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1680 1452 489 385 367 346 104 91 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3659 3659 Size: 7006 BtgI C'CryG_G Cuts at: 513 1065 2081 3447 3843 4298 513 Size: 552 1016 1366 396 455 3221 Fragments arranged by size: 3221 1366 1016 552 455 396 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6267 Fragments arranged by size: 6267 683 56 BtsI GCAGTG_nn' Cuts at: 2850 3693 4522 5316 5336 2850 Size: 843 829 794 20 4520 Fragments arranged by size: 4520 843 829 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7006 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6971 DraI TTT'AAA Cuts at: 2571 4113 4310 2571 Size: 1542 197 5267 Fragments arranged by size: 5267 1542 197 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6013 809 Size: 507 4697 1802 Fragments arranged by size: 4697 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3444 4271 4453 Size: 54 1425 50 275 18 1614 827 182 Cuts at: 4453 5342 6971 8 Size: 889 1629 43 Fragments arranged by size: 1629 1614 1425 889 827 275 182 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4271 4453 1537 Size: 275 2459 182 4090 Fragments arranged by size: 4090 2459 275 182 EarI CTCTTCn'nnn_ Cuts at: 1353 2031 2721 2912 3480 3998 4936 1353 Size: 678 690 191 568 518 938 3423 Fragments arranged by size: 3423 938 690 678 568 518 191 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3689 5593 5967 6113 387 Size: 1064 138 2100 1904 374 146 1280 Fragments arranged by size: 2100 1904 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2806 3163 3943 3991 2806 Size: 357 780 48 5821 Fragments arranged by size: 5821 780 357 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2143 3080 3101 3236 3290 3344 Size: 393 407 104 937 21 135 54 54 Cuts at: 3344 3428 3482 3554 3575 3617 3671 3917 3959 Size: 84 54 72 21 42 54 246 42 Cuts at: 3959 4238 5069 5666 6453 6721 1239 Size: 279 831 597 787 268 1524 Fragments arranged by size: 1524 937 831 787 597 407 393 279 268 246 135 104 84 72 54 54 54 54 42 42 21 21 EcoNI CCTnn'n_nnAGG Cuts at: 3464 3464 Size: 7006 EcoO109I rG'GnC_Cy Cuts at: 3205 3655 3663 3706 3724 3787 3205 Size: 450 8 43 18 63 6424 Fragments arranged by size: 6424 450 63 43 18 8 EcoRI G'AATT_C Cuts at: 4315 4693 4315 Size: 378 6628 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2291 2291 Size: 7006 FspI TGC'GCA Cuts at: 5512 5512 Size: 7006 HaeII r_GCGC'y Cuts at: 1678 3248 4040 6153 1678 Size: 1570 792 2113 2531 Fragments arranged by size: 2531 2113 1570 792 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2159 2191 2473 2505 3000 Size: 32 135 32 587 32 282 32 495 Cuts at: 3000 3032 5647 5679 5721 5753 1373 Size: 32 2615 32 42 32 2626 Fragments arranged by size: 2626 2615 587 495 282 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4350 4596 669 Size: 988 281 2412 246 3079 Fragments arranged by size: 3079 2412 988 281 246 HindIII A'AGCT_T Cuts at: 748 2273 748 Size: 1525 5481 HpaI GTT'AAC Cuts at: 4596 4596 Size: 7006 KasI G'GCGC_C Cuts at: 4036 4036 Size: 7006 KpnI G_GTAC'C Cuts at: 4331 4331 Size: 7006 MfeI C'AATT_G Cuts at: 4605 4605 Size: 7006 MluI A'CGCG_T Cuts at: 6882 6882 Size: 7006 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3139 3830 6120 6304 1305 Size: 1834 691 2290 184 2007 Fragments arranged by size: 2290 2007 1834 691 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3446 10 Size: 54 1425 343 1614 3570 Fragments arranged by size: 3570 1614 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4964 5323 5482 518 Size: 648 3798 359 159 2042 Fragments arranged by size: 3798 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3287 3595 3637 3931 4422 5099 6247 6492 Size: 1681 308 42 294 491 677 1148 245 Cuts at: 6492 1606 Size: 2120 Fragments arranged by size: 2120 1681 1148 677 491 308 294 245 42 NarI GG'CG_CC Cuts at: 4037 4037 Size: 7006 NcoI C'CATG_G Cuts at: 513 1065 3447 3843 4298 513 Size: 552 2382 396 455 3221 Fragments arranged by size: 3221 2382 552 455 396 NdeI CA'TA_TG Cuts at: 387 387 Size: 7006 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7006 NotI GC'GGCC_GC Cuts at: 4453 4453 Size: 7006 NruI TCG'CGA Cuts at: 4131 4131 Size: 7006 NspI r_CATG'y Cuts at: 1132 1229 2566 4106 4204 4364 5909 1132 Size: 97 1337 1540 98 160 1545 2229 Fragments arranged by size: 2229 1545 1540 1337 160 98 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7006 PciI A'CATG_T Cuts at: 4102 5905 4102 Size: 1803 5203 PflMI CCAn_nnn'nTGG Cuts at: 1108 3262 1108 Size: 2154 4852 PfoI T'CCnGG_A Cuts at: 4002 4002 Size: 7006 PmeI GTTT'AAAC Cuts at: 4310 4310 Size: 7006 PmlI CAC'GTG Cuts at: 2953 3766 2953 Size: 813 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2790 2822 5075 5107 6588 6620 2790 Size: 32 2253 32 1481 32 3176 Fragments arranged by size: 3176 2253 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3663 3706 3787 3663 Size: 43 81 6882 Fragments arranged by size: 6882 81 43 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7006 PsiI TTA'TAA Cuts at: 4154 4576 4154 Size: 422 6584 PspOMI G'GGCC_C Cuts at: 3724 3724 Size: 7006 PspXI vC'TCGA_Gb Cuts at: 3772 3772 Size: 7006 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3360 3392 3360 Size: 32 6974 PstI C_TGCA'G Cuts at: 830 3627 3642 3688 3814 3960 4358 830 Size: 2797 15 46 126 146 398 3478 Fragments arranged by size: 3478 2797 398 146 126 46 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7006 PvuII CAG'CTG Cuts at: 3287 3595 3637 3287 Size: 308 42 6656 Fragments arranged by size: 6656 308 42 SacI G_AGCT'C Cuts at: 2808 3165 3945 3993 2808 Size: 357 780 48 5821 Fragments arranged by size: 5821 780 357 48 SalI G'TCGA_C Cuts at: 1936 4348 1936 Size: 2412 4594 SapI GCTCTTCn'nnn_ Cuts at: 2721 3998 2721 Size: 1277 5729 SbfI CC_TGCA'GG Cuts at: 4358 4358 Size: 7006 ScaI AGT'ACT Cuts at: 5254 6966 5254 Size: 1712 5294 SfcI C'TryA_G Cuts at: 826 1045 3623 3638 3684 3810 3860 3956 4354 Size: 219 2578 15 46 126 50 96 398 Cuts at: 4354 5489 6170 6361 826 Size: 1135 681 191 1471 Fragments arranged by size: 2578 1471 1135 681 398 219 191 126 96 50 46 15 SfoI GGC'GCC Cuts at: 4038 4038 Size: 7006 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7006 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7006 SmaI CCC'GGG Cuts at: 1872 4333 1872 Size: 2461 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2680 2862 3453 3668 3772 4205 Size: 197 926 737 182 591 215 104 433 Cuts at: 4205 5110 6011 6273 820 Size: 905 901 262 1553 Fragments arranged by size: 1553 926 905 901 737 591 433 262 215 197 182 104 SnaBI TAC'GTA Cuts at: 493 4797 493 Size: 4304 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7006 SphI G_CATG'C Cuts at: 1132 4364 1132 Size: 3232 3774 SspI AAT'ATT Cuts at: 5 52 2446 4930 5 Size: 47 2394 2484 2081 Fragments arranged by size: 2484 2394 2081 47 StuI AGG'CCT Cuts at: 1517 3098 1517 Size: 1581 5425 StyI C'CwwG_G Cuts at: 513 1065 2910 3447 3843 4257 4298 4445 513 Size: 552 1845 537 396 414 41 147 3074 Fragments arranged by size: 3074 1845 552 537 414 396 147 41 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3254 5033 5050 5203 5388 5833 325 Size: 1255 1674 1779 17 153 185 445 1498 Fragments arranged by size: 1779 1674 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5252 6964 96 Size: 274 80 33 51 1443 3275 1712 138 Fragments arranged by size: 3275 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6966 6998 6966 Size: 32 6974 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7006 XbaI T'CTAG_A Cuts at: 4342 4342 Size: 7006 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3055 3055 Size: 7006 XhoI C'TCGA_G Cuts at: 1943 3772 1943 Size: 1829 5177 XmaI C'CCGG_G Cuts at: 1870 4331 1870 Size: 2461 4545 XmnI GAAnn'nnTTC Cuts at: 2492 3564 5135 2492 Size: 1072 1571 4363 Fragments arranged by size: 4363 1571 1072 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4814 276 Size: 53 83 186 936 3280 2468 Fragments arranged by size: 3280 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NsiI PacI RsrII SacII SanDI SexAI SfiI SrfI SwaI