################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I Fnu4HI FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MaeI MaeIII MamI MfeI MluI MluNI MscI MseI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I TseI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 396 TCCGGA T^CCGGA AcsI 205 AAATTC [GA]^AATT[CT] AlwI 36 GGATC GGATC(4/5) ApoI 205 AAATTC [GA]^AATT[CT] Asp700I 144 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 158 TGCGCA TGC^GCA BanII 355 GAGCCC G[GA]GC[CT]^C BciVI 180 GTATCC GTATCC(6/5) BclI 200 TGATCA T^GATCA BglII 218 AGATCT A^GATCT BmyI 355 GAGCCC G[AGT]GC[ACT]^C BsaWI 396 TCCGGA [AT]^CCGG[AT] BseAI 396 TCCGGA T^CCGGA BseRI 112 CTCCTC GAGGAG(10/8) BsmI 414 GAATGC GAATGC(1/-1) Bsp1286I 355 GAGCCC G[AGT]GC[ACT]^C BspEI 396 TCCGGA T^CCGGA BsrFI 449 ACCGGC [GA]^CCGG[CT] BsrI 281 ACTGG ACTGG(1/-1) BstF5I 365 GGATG GGATG(2/0) BstXI 89 CCAATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 159 GCGC GCG^C Cfr10I 449 ACCGGC [GA]^CCGG[CT] DraII 317 AGGCCCT [GA]G^G[ACGT]CC[CT] EarI 421 GAAGAG CTCTTC(1/4) EcoO109I 317 AGGCCCT [GA]G^G[ACGT]CC[CT] FauI 171 CCCGC CCCGC(4/6) FbaI 200 TGATCA T^GATCA FokI 365 GGATG GGATG(9/13) FspI 158 TGCGCA TGC^GCA HhaI 159 GCGC GCG^C HinP1I 159 GCGC G^CGC Ksp632I 421 GAAGAG CTCTTC(1/4) MaeII 233 ACGT A^CGT MroI 396 TCCGGA T^CCGGA MslI 12 CATGCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 11 GCATGC [GA]CATG^[CT] PflMI 306 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SphI 11 GCATGC GCATG^C Tsp509I 206 AATT ^AATT TspRI 273 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 306 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 144 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AvaII 369 GGACC G^G[AT]CC 447 GGACC BpmI 31 CTGGAG CTGGAG(16/14) 313 CTGGAG BssKI 96 CCTGG ^CC[ACGT]GG 312 CCTGG BstNI 96 CCTGG CC^[AT]GG 312 CCTGG BstYI 36 GGATCT [GA]^GATC[CT] 218 AGATCT EcoRII 96 CCTGG ^CC[AT]GG 312 CCTGG HaeIII 318 GGCC GG^CC 452 GGCC HinfI 393 GAATC G^A[ACGT]TC 437 GATTC MflI 36 GGATCT [GA]^GATC[CT] 218 AGATCT MvaI 96 CCTGG CC^[AT]GG 312 CCTGG NlaIII 12 CATG CATG^ 198 CATG NlaIV 68 GGAACC GG[ACGT]^[ACGT]CC 354 GGAGCC ScrFI 96 CCTGG CC^[ACGT]GG 312 CCTGG SfaNI 85 GATGC GCATC(5/9) 161 GCATC TfiI 393 GAATC G^A[AT]TC 437 GATTC XhoII 36 GGATCT [GA]^GATC[CT] 218 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AciI AluI BsiYI BslI BspMI Cac8I Cfr13I DpnII HapII HpaII HphI MboI MboII MnlI MspI NdeII Sau3AI Sau96I