(Linear) (Six-base) MAPSORT of: KIEE3593.seq Check: 6800 from: 1 to: 459 KIEE3593.orf With 182 enzymes: SgfI * December 27, 2011 16:35 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 459 Size: 25 434 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 169 201 459 Size: 169 32 258 Fragments arranged by size: 258 169 32 ApoI r'AATT_y Cuts at: 0 205 459 Size: 205 254 BanII G_rGCy'C Cuts at: 0 359 459 Size: 359 100 BciVI GTATCCnnnnn_n' Cuts at: 0 191 459 Size: 191 268 BclI T'GATC_A Cuts at: 0 200 459 Size: 200 259 BglII A'GATC_T Cuts at: 0 218 459 Size: 218 241 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 334 459 Size: 52 282 125 Fragments arranged by size: 282 125 52 BsaWI w'CCGG_w Cuts at: 0 396 459 Size: 396 63 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 169 199 439 459 Size: 169 30 240 20 Fragments arranged by size: 240 169 30 20 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 103 459 Size: 103 356 BsmI GAATG_Cn' Cuts at: 0 420 459 Size: 420 39 Bsp1286I G_dGCh'C Cuts at: 0 359 459 Size: 359 100 BspEI T'CCGG_A Cuts at: 0 396 459 Size: 396 63 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 71 164 389 459 Size: 71 93 225 70 Fragments arranged by size: 225 93 71 70 BsrFI r'CCGG_y Cuts at: 0 449 459 Size: 449 10 BstXI CCAn_nnnn'nTGG Cuts at: 0 96 459 Size: 96 363 BstYI r'GATC_y Cuts at: 0 36 218 459 Size: 36 182 241 Fragments arranged by size: 241 182 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 95 459 Size: 95 364 BtsI GCAGTG_nn' Cuts at: 0 279 459 Size: 279 180 EarI CTCTTCn'nnn_ Cuts at: 0 416 459 Size: 416 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 52 334 459 Size: 25 27 282 125 Fragments arranged by size: 282 125 27 25 EcoO109I rG'GnC_Cy Cuts at: 0 318 459 Size: 318 141 FspI TGC'GCA Cuts at: 0 160 459 Size: 160 299 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 332 364 378 410 439 459 Size: 332 32 14 32 29 20 Fragments arranged by size: 332 32 32 29 20 14 MslI CAynn'nnrTG Cuts at: 0 16 459 Size: 16 443 NspI r_CATG'y Cuts at: 0 15 459 Size: 15 444 PflMI CCAn_nnn'nTGG Cuts at: 0 312 459 Size: 312 147 PfoI T'CCnGG_A Cuts at: 0 311 459 Size: 311 148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 169 201 459 Size: 169 32 258 Fragments arranged by size: 258 169 32 SphI G_CATG'C Cuts at: 0 15 459 Size: 15 444 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 357 459 Size: 357 102 XmnI GAAnn'nnTTC Cuts at: 0 148 459 Size: 148 311 Enzymes that do cut: AcuI AloI ApoI BanII BciVI BclI BglII BpmI BsaWI BsaXI BseRI BsmI Bsp1286I BspEI BspMI BsrFI BstXI BstYI BtgZI BtsI EarI Eco57MI EcoO109I FspI Hin4I MslI NspI PflMI PfoI PpiI SphI TaqII XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI