##################################
# NO CUT ENZYMES (  189 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaII       AviII       AvrII       
BalI        BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BclI        BcnI        
BfrI        BlnI        BlpI        BmyI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     FbaI        FseI        FspI        HaeII       
HapII       HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        KspI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        Tru9I       Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AocI          211  CCTAAGG              CC^T[ACGT]AGG
BamHI         117  GGATCC               G^GATCC
BciVI         345  GTATCC               GTATCC(6/5)
BglI           25  GCCATCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         123  AGATCT               A^GATCT
BsmAI         144  GAGAC                GTCTC(1/5)
Bsu36I        211  CCTAAGG              CC^T[ACGT]AGG
Cac8I         199  GCTAGC               GC[ACGT]^[ACGT]GC
Cfr13I        102  GGGCC                G^G[ACGT]CC
EarI          148  CTCTTC               CTCTTC(1/4)
Eco81I        211  CCTAAGG              CC^T[ACGT]AGG
EcoNI         189  CCTGAGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       244  ATGCAT               ATGCA^T
FauI           98  GCGGG                CCCGC(4/6)
HgaI          281  GCGTC                GACGC(5/10)
Ksp632I       148  CTCTTC               CTCTTC(1/4)
MaeIII        239  GTGAC                ^GT[ACGT]AC
MboII         149  TCTTC                GAAGA(8/7)
MspA1I        133  CAGCTG               C[AC]G^C[GT]G
NheI          199  GCTAGC               G^CTAGC
NsiI          244  ATGCAT               ATGCA^T
NspI          242  ACATGC               [GA]CATG^[CT]
PvuII         133  CAGCTG               CAG^CTG
Sau96I        102  GGGCC                G^G[ACGT]CC
SfaNI         335  GCATC                GCATC(5/9)
SmlI          340  CTCAAG               C^T[CT][GA]AG
Tsp45I        239  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AciI          105  CCGC                 CCGC(-3/-1)
               98  GCGG                
AvaI          229  CCCGAG               C^[CT]CG[GA]G
              351  CCCGAG              
BfaI           43  CTAG                 C^TAG
              200  CTAG                
BpmI          139  CTCCAG               CTGGAG(16/14)
              169  CTGGAG              
BsaBI         118  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              304  GATGTGAATC          
BseRI          82  GAGGAG               GAGGAG(10/8)
              276  GAGGAG              
BsmFI         261  GGGAC                GGGAC(10/14)
              322  GTCCC               
BsoBI         229  CCCGAG               C^[CT]CG[GA]G
              351  CCCGAG              
HaeIII         33  GGCC                 GG^CC
              103  GGCC                
MaeI           43  CTAG                 C^TAG
              200  CTAG                
MaeII         237  ACGT                 A^CGT
              320  ACGT                
MamI          118  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              304  GATGTGAATC          
PleI          146  GACTC                GAGTC(4/5)
              292  GACTC               
TfiI          217  GAATC                G^A[AT]TC
              309  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AluI        AlwI        BbvI        BsiYI       BslI        
BssKI       BstF5I      BstNI       BstYI       DdeI        
DpnII       EcoRII      Fnu4HI      FokI        HinfI       
ItaI        MboI        MflI        MnlI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       Sau3AI      
ScrFI       TseI        XhoII