(Circular) (Six-base) MAPSORT of: pFN21AE4510.seq Check: 6576 from: 1 to: 6301 pFN21AE4510 6301 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2099 2099 Size: 6301 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4111 278 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3644 1937 Size: 1707 4594 Acc65I G'GTAC_C Cuts at: 3622 3622 Size: 6301 AclI AA'CG_TT Cuts at: 1563 4428 4801 1563 Size: 2865 373 3063 Fragments arranged by size: 3063 2865 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 3503 3569 4364 5748 6016 1632 Size: 695 1176 66 795 1384 268 1917 Fragments arranged by size: 1917 1384 1176 795 695 268 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6104 AflIII A'CryG_T Cuts at: 1203 5200 6177 1203 Size: 3997 977 1327 Fragments arranged by size: 3997 1327 977 AgeI A'CCGG_T Cuts at: 3477 5132 3477 Size: 1655 4646 AhdI GACnn_n'nnGTC Cuts at: 3157 5030 3157 Size: 1873 4428 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6301 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2802 1184 Size: 1618 4683 AlwNI CAG_nnn'CTG Cuts at: 2064 4044 5616 2064 Size: 1980 1572 2749 Fragments arranged by size: 2749 1980 1572 ApaI G_GGCC'C Cuts at: 3431 3431 Size: 6301 ApaLI G'TGCA_C Cuts at: 4358 5514 4358 Size: 1156 5145 ApoI r'AATT_y Cuts at: 1493 2129 2584 2918 3610 3836 3988 6097 1493 Size: 636 455 334 692 226 152 2109 1697 Fragments arranged by size: 2109 1697 692 636 455 334 226 152 AseI AT'TA_AT Cuts at: 160 1032 3231 4855 160 Size: 872 2199 1624 1606 Fragments arranged by size: 2199 1624 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 3626 1870 Size: 73 1683 4545 Fragments arranged by size: 4545 1683 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2316 2349 2316 Size: 33 6268 BamHI G'GATC_C Cuts at: 3631 3998 3631 Size: 367 5934 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3622 5077 618 Size: 325 217 627 1835 1455 1842 Fragments arranged by size: 1842 1835 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2088 2868 3431 3620 1391 Size: 697 780 563 189 4072 Fragments arranged by size: 4072 780 697 563 189 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3219 928 Size: 971 1320 4010 Fragments arranged by size: 4010 1320 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4492 4526 1903 Size: 34 2555 34 3678 Fragments arranged by size: 3678 2555 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2344 2469 4193 5403 2344 Size: 125 1724 1210 3242 Fragments arranged by size: 3242 1724 1210 125 BclI T'GATC_A Cuts at: 1548 2637 1548 Size: 1089 5212 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4912 136 Size: 107 122 71 631 605 3240 1525 Fragments arranged by size: 3240 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6296 6296 Size: 6301 BlpI GC'TnA_GC Cuts at: 3718 3718 Size: 6301 Bme1580I G_kGCm'C Cuts at: 1790 3431 4362 5518 1790 Size: 1641 931 1156 2573 Fragments arranged by size: 2573 1641 1156 931 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6301 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3463 4032 4990 Size: 416 53 83 399 24 2046 569 958 Cuts at: 4990 442 Size: 1753 Fragments arranged by size: 2046 1753 958 569 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2163 1056 Size: 1107 5194 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 3140 4961 1239 Size: 863 1038 1821 2579 Fragments arranged by size: 2579 1821 1038 863 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2717 3107 4426 5291 5589 2717 Size: 390 1319 865 298 3429 Fragments arranged by size: 3429 1319 865 390 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2921 3269 4964 882 Size: 617 1422 348 1695 2219 Fragments arranged by size: 2219 1695 1422 617 348 BsaAI yAC'GTr Cuts at: 493 1204 4092 493 Size: 711 2888 2702 Fragments arranged by size: 2888 2702 711 BsaBI GATnn'nnATC Cuts at: 6295 6295 Size: 6301 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4108 4490 5125 275 Size: 53 83 186 936 2575 382 635 1451 Fragments arranged by size: 2575 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2444 3477 4734 5132 5406 5553 5964 2444 Size: 1033 1257 398 274 147 411 2781 Fragments arranged by size: 2781 1257 1033 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2022 2052 1178 Size: 30 165 30 619 30 5427 Fragments arranged by size: 5427 619 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2162 3069 3307 1186 Size: 976 907 238 4180 Fragments arranged by size: 4180 976 907 238 BseYI C'CCAG_C Cuts at: 2869 3165 5504 6000 2869 Size: 296 2339 496 3170 Fragments arranged by size: 3170 2339 496 296 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5728 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3482 3751 4512 5540 1540 Size: 275 186 1481 269 761 1028 2301 Fragments arranged by size: 2301 1481 1028 761 275 269 186 BsiHKAI G_wGCw'C Cuts at: 2868 4362 4447 5518 2868 Size: 1494 85 1071 3651 Fragments arranged by size: 3651 1494 1071 85 BsmI GAATG_Cn' Cuts at: 2791 2877 3812 3905 2791 Size: 86 935 93 5187 Fragments arranged by size: 5187 935 93 86 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2868 3431 3620 4362 4447 5518 Size: 399 298 780 563 189 742 85 1071 Cuts at: 5518 1391 Size: 2174 Fragments arranged by size: 2174 1071 780 742 563 399 298 189 85 BspEI T'CCGG_A Cuts at: 2444 5964 2444 Size: 3520 2781 BspHI T'CATG_A Cuts at: 2634 4190 5920 2634 Size: 1556 1730 3015 Fragments arranged by size: 3015 1730 1556 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2099 3656 844 Size: 1255 1557 3489 Fragments arranged by size: 3489 1557 1255 BsrBI CCG'CTC Cuts at: 1394 1823 4188 1394 Size: 429 2365 3507 Fragments arranged by size: 3507 2365 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2113 2277 4796 4970 65 Size: 1183 865 164 2519 174 1396 Fragments arranged by size: 2519 1396 1183 865 174 164 BsrFI r'CCGG_y Cuts at: 1700 1810 3477 4945 5132 1700 Size: 110 1667 1468 187 2869 Fragments arranged by size: 2869 1667 1468 187 110 BsrGI T'GTAC_A Cuts at: 96 2769 96 Size: 2673 3628 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6301 BssSI C'ACGA_G Cuts at: 3501 4361 5373 3501 Size: 860 1012 4429 Fragments arranged by size: 4429 1012 860 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6301 BstBI TT'CG_AA Cuts at: 5105 5105 Size: 6301 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6301 BstXI CCAn_nnnn'nTGG Cuts at: 3128 3438 3128 Size: 310 5991 BstYI r'GATC_y Cuts at: 1972 2262 3364 3631 3998 4383 4400 5852 5938 Size: 290 1102 267 367 385 17 1452 86 Cuts at: 5938 5950 6296 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1102 385 367 346 290 267 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2837 2837 Size: 6301 BtgI C'CryG_G Cuts at: 513 1065 2004 3347 3378 513 Size: 552 939 1343 31 3436 Fragments arranged by size: 3436 1343 939 552 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5562 Fragments arranged by size: 5562 683 56 BtsI GCAGTG_nn' Cuts at: 3111 3443 3817 4611 4631 3111 Size: 332 374 794 20 4781 Fragments arranged by size: 4781 794 374 332 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2077 2112 529 Size: 35 1513 35 4718 Fragments arranged by size: 4718 1513 35 35 DraI TTT'AAA Cuts at: 2503 2826 3064 3605 2503 Size: 323 238 541 5199 Fragments arranged by size: 5199 541 323 238 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5308 809 Size: 507 3992 1802 Fragments arranged by size: 3992 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2371 2934 Size: 54 1425 50 275 18 189 352 563 Cuts at: 2934 3375 3440 3748 4637 6266 8 Size: 441 65 308 889 1629 43 Fragments arranged by size: 1629 1425 889 563 441 352 308 275 189 65 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3748 1537 Size: 275 1936 4090 Fragments arranged by size: 4090 1936 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 2096 2194 4231 1353 Size: 714 29 98 2037 3423 Fragments arranged by size: 3423 2037 714 98 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4888 5262 5408 387 Size: 1064 138 3299 374 146 1280 Fragments arranged by size: 3299 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2866 2866 Size: 6301 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2327 3140 3503 3569 4364 4961 Size: 393 470 225 813 363 66 795 597 Cuts at: 4961 5748 6016 1239 Size: 787 268 1524 Fragments arranged by size: 1524 813 795 787 597 470 393 363 268 225 66 EcoNI CCTnn'n_nnAGG Cuts at: 2056 2056 Size: 6301 EcoO109I rG'GnC_Cy Cuts at: 2119 2119 Size: 6301 EcoRI G'AATT_C Cuts at: 2584 2918 3610 3988 2584 Size: 334 692 378 4897 Fragments arranged by size: 4897 692 378 334 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3547 3547 Size: 6301 FspI TGC'GCA Cuts at: 4807 4807 Size: 6301 HaeII r_GCGC'y Cuts at: 1678 5448 1678 Size: 3770 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3143 3175 3299 3331 4942 Size: 32 135 32 1571 32 124 32 1611 Cuts at: 4942 4974 5016 5048 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1611 1571 135 124 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3096 3151 3597 3645 3891 669 Size: 988 281 1158 55 446 48 246 3079 Fragments arranged by size: 3079 1158 988 446 281 246 55 48 HindIII A'AGCT_T Cuts at: 748 748 Size: 6301 HpaI GTT'AAC Cuts at: 3891 3891 Size: 6301 KpnI G_GTAC'C Cuts at: 3626 3626 Size: 6301 MfeI C'AATT_G Cuts at: 3900 3900 Size: 6301 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2208 5415 5599 1305 Size: 903 3207 184 2007 Fragments arranged by size: 3207 2007 903 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2021 2373 3377 3442 10 Size: 54 1425 343 189 352 1004 65 2869 Fragments arranged by size: 2869 1425 1004 352 343 189 65 54 MslI CAynn'nnrTG Cuts at: 518 1166 3104 4259 4618 4777 518 Size: 648 1938 1155 359 159 2042 Fragments arranged by size: 2042 1938 1155 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3165 3717 4394 5542 5787 1606 Size: 1559 552 677 1148 245 2120 Fragments arranged by size: 2120 1559 1148 677 552 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3347 3378 513 Size: 552 939 1343 31 3436 Fragments arranged by size: 3436 1343 939 552 31 NdeI CA'TA_TG Cuts at: 387 387 Size: 6301 NheI G'CTAG_C Cuts at: 1052 2159 1052 Size: 1107 5194 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NspI r_CATG'y Cuts at: 1132 1229 2704 3659 5204 1132 Size: 97 1475 955 1545 2229 Fragments arranged by size: 2229 1545 1475 955 97 PasI CC'CwG_GG Cuts at: 1348 3431 1348 Size: 2083 4218 PciI A'CATG_T Cuts at: 5200 5200 Size: 6301 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6301 PfoI T'CCnGG_A Cuts at: 2265 2485 2265 Size: 220 6081 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4370 4402 5883 5915 4370 Size: 32 1481 32 4756 Fragments arranged by size: 4756 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6301 PsiI TTA'TAA Cuts at: 3871 3871 Size: 6301 PspOMI G'GGCC_C Cuts at: 3427 3427 Size: 6301 PstI C_TGCA'G Cuts at: 830 3653 830 Size: 2823 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6301 SacI G_AGCT'C Cuts at: 2868 2868 Size: 6301 SalI G'TCGA_C Cuts at: 1936 3643 1936 Size: 1707 4594 SapI GCTCTTCn'nnn_ Cuts at: 2096 2096 Size: 6301 SbfI CC_TGCA'GG Cuts at: 3653 3653 Size: 6301 ScaI AGT'ACT Cuts at: 4549 6261 4549 Size: 1712 4589 SexAI A'CCwGG_T Cuts at: 2539 2539 Size: 6301 SfcI C'TryA_G Cuts at: 826 1045 3649 4784 5465 5656 826 Size: 219 2604 1135 681 191 1471 Fragments arranged by size: 2604 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 3628 1872 Size: 1756 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2732 3086 4405 5306 5568 820 Size: 197 926 789 354 1319 901 262 1553 Fragments arranged by size: 1553 1319 926 901 789 354 262 197 SnaBI TAC'GTA Cuts at: 493 4092 493 Size: 3599 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 3659 1132 Size: 2527 3774 SspI AAT'ATT Cuts at: 5 52 4225 5 Size: 47 4173 2081 Fragments arranged by size: 4173 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6301 StyI C'CwwG_G Cuts at: 513 1065 2004 2693 3127 3347 3378 3740 513 Size: 552 939 689 434 220 31 362 3074 Fragments arranged by size: 3074 939 689 552 434 362 220 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2650 4328 4345 4498 4683 5128 325 Size: 1255 1070 1678 17 153 185 445 1498 Fragments arranged by size: 1678 1498 1255 1070 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2310 2769 3194 Size: 274 80 33 51 1443 333 459 425 Cuts at: 3194 4547 6259 96 Size: 1353 1712 138 Fragments arranged by size: 1712 1443 1353 459 425 333 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2022 2054 2439 2471 6261 6293 2022 Size: 32 385 32 3790 32 2030 Fragments arranged by size: 3790 2030 385 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6301 XbaI T'CTAG_A Cuts at: 3637 3637 Size: 6301 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3376 3376 Size: 6301 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6301 XmaI C'CCGG_G Cuts at: 1870 3626 1870 Size: 1756 4545 XmnI GAAnn'nnTTC Cuts at: 2918 4430 2918 Size: 1512 4789 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4109 276 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI