(Circular) (Six-base) MAPSORT of: pFN21AB9925.seq Check: 5332 from: 1 to: 5743 pFN21AB9925 5743 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3553 278 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3086 1937 Size: 1149 4594 Acc65I G'GTAC_C Cuts at: 3064 3064 Size: 5743 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2387 2468 3806 5190 5458 1632 Size: 755 81 1338 1384 268 1917 Fragments arranged by size: 1917 1384 1338 755 268 81 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5546 AflIII A'CryG_T Cuts at: 1203 2913 4642 5619 1203 Size: 1710 1729 977 1327 Fragments arranged by size: 1729 1710 1327 977 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 4472 4472 Size: 5743 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5743 AlwNI CAG_nnn'CTG Cuts at: 2783 3486 5058 2783 Size: 703 1572 3468 Fragments arranged by size: 3468 1572 703 ApaLI G'TGCA_C Cuts at: 3800 4956 3800 Size: 1156 4587 ApoI r'AATT_y Cuts at: 1493 2548 2682 3052 3278 3430 5539 1493 Size: 1055 134 370 226 152 2109 1697 Fragments arranged by size: 2109 1697 1055 370 226 152 134 AseI AT'TA_AT Cuts at: 160 1032 4297 160 Size: 872 3265 1606 Fragments arranged by size: 3265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 2016 2760 3068 1870 Size: 73 73 744 308 4545 Fragments arranged by size: 4545 744 308 73 73 BamHI G'GATC_C Cuts at: 3073 3440 3073 Size: 367 5376 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3064 4519 618 Size: 325 217 627 1277 1455 1842 Fragments arranged by size: 1842 1455 1277 627 325 217 BanII G_rGCy'C Cuts at: 1391 2853 3062 1391 Size: 1462 209 4072 Fragments arranged by size: 4072 1462 209 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2579 928 Size: 971 680 4092 Fragments arranged by size: 4092 971 680 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2729 2763 3934 3968 1903 Size: 34 792 34 1171 34 3678 Fragments arranged by size: 3678 1171 792 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3635 4845 3635 Size: 1210 4533 BclI T'GATC_A Cuts at: 1548 2428 1548 Size: 880 4863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4354 136 Size: 107 122 71 631 605 2682 1525 Fragments arranged by size: 2682 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2183 2886 5738 2183 Size: 703 2852 2188 Fragments arranged by size: 2852 2188 703 BlpI GC'TnA_GC Cuts at: 3160 3160 Size: 5743 Bme1580I G_kGCm'C Cuts at: 1790 2395 3804 4960 1790 Size: 605 1409 1156 2573 Fragments arranged by size: 2573 1409 1156 605 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5743 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2130 3474 4432 Size: 416 53 83 399 24 713 1344 958 Cuts at: 4432 442 Size: 1753 Fragments arranged by size: 1753 1344 958 713 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5743 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2867 3027 4403 1239 Size: 1628 160 1376 2579 Fragments arranged by size: 2579 1628 1376 160 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1995 3868 4733 5031 1995 Size: 1873 865 298 2707 Fragments arranged by size: 2707 1873 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2495 4406 882 Size: 617 996 1911 2219 Fragments arranged by size: 2219 1911 996 617 BsaAI yAC'GTr Cuts at: 493 1204 3534 493 Size: 711 2330 2702 Fragments arranged by size: 2702 2330 711 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3550 3932 4567 275 Size: 53 83 186 936 2017 382 635 1451 Fragments arranged by size: 2017 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4176 4574 4848 4995 5406 4176 Size: 398 274 147 411 4513 Fragments arranged by size: 4513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3000 3030 1178 Size: 30 165 30 1597 30 3891 Fragments arranged by size: 3891 1597 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2130 2917 1186 Size: 913 31 787 4012 Fragments arranged by size: 4012 913 787 31 BseYI C'CCAG_C Cuts at: 4946 5442 4946 Size: 496 5247 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2210 2577 2781 1163 Size: 573 474 367 204 4125 Fragments arranged by size: 4125 573 474 367 204 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3193 3954 4982 1540 Size: 275 186 1192 761 1028 2301 Fragments arranged by size: 2301 1192 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2853 3038 3804 3889 4960 2853 Size: 185 766 85 1071 3636 Fragments arranged by size: 3636 1071 766 185 85 BsmI GAATG_Cn' Cuts at: 2750 3254 3347 2750 Size: 504 93 5146 Fragments arranged by size: 5146 504 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2395 2853 3038 3062 3804 3889 4960 Size: 399 605 458 185 24 742 85 1071 Cuts at: 4960 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 605 458 399 185 85 24 BspEI T'CCGG_A Cuts at: 5406 5406 Size: 5743 BspHI T'CATG_A Cuts at: 2250 3632 5362 2250 Size: 1382 1730 2631 Fragments arranged by size: 2631 1730 1382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3098 844 Size: 2254 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3630 1394 Size: 429 1807 3507 Fragments arranged by size: 3507 1807 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2868 4238 4412 65 Size: 1183 1620 1370 174 1396 Fragments arranged by size: 1620 1396 1370 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4387 4574 1700 Size: 110 2577 187 2869 Fragments arranged by size: 2869 2577 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5743 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5743 BssSI C'ACGA_G Cuts at: 3803 4815 3803 Size: 1012 4731 BstAPI GCAn_nnn'nTGC Cuts at: 794 2458 794 Size: 1664 4079 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5743 BstYI r'GATC_y Cuts at: 1972 2183 2886 3073 3440 3825 3842 5294 5380 Size: 211 703 187 367 385 17 1452 86 Cuts at: 5380 5392 5738 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 703 385 367 346 211 187 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5191 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5004 Fragments arranged by size: 5004 683 56 BtsI GCAGTG_nn' Cuts at: 2040 2307 2395 3259 4053 4073 2040 Size: 267 88 864 794 20 3710 Fragments arranged by size: 3710 864 794 267 88 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5708 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4750 809 Size: 507 3434 1802 Fragments arranged by size: 3434 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3190 4079 5708 Size: 54 1425 50 275 18 1360 889 1629 Cuts at: 5708 8 Size: 43 Fragments arranged by size: 1629 1425 1360 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3190 1537 Size: 275 1378 4090 Fragments arranged by size: 4090 1378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2959 3673 1353 Size: 1606 714 3423 Fragments arranged by size: 3423 1606 714 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4330 4704 4850 387 Size: 1064 138 2741 374 146 1280 Fragments arranged by size: 2741 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2851 2851 Size: 5743 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2387 2468 2867 3027 3806 4403 5190 Size: 393 755 81 399 160 779 597 787 Cuts at: 5190 5458 1239 Size: 268 1524 Fragments arranged by size: 1524 787 779 755 597 399 393 268 160 81 EcoRI G'AATT_C Cuts at: 3052 3430 3052 Size: 378 5365 EcoRV GAT'ATC Cuts at: 2311 2311 Size: 5743 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 4890 1678 Size: 3212 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3000 3032 4384 4416 4458 Size: 32 135 32 1428 32 1352 32 42 Cuts at: 4458 4490 1373 Size: 32 2626 Fragments arranged by size: 2626 1428 1352 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2832 3087 3333 669 Size: 988 281 894 255 246 3079 Fragments arranged by size: 3079 988 894 281 255 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5743 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KpnI G_GTAC'C Cuts at: 3068 3068 Size: 5743 MfeI C'AATT_G Cuts at: 3342 3342 Size: 5743 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4857 5041 1305 Size: 3552 184 2007 Fragments arranged by size: 3552 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3921 Fragments arranged by size: 3921 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2805 2918 3701 4060 4219 518 Size: 648 1639 113 783 359 159 2042 Fragments arranged by size: 2042 1639 783 648 359 159 113 MspA1I CmG'CkG Cuts at: 1606 3159 3836 4984 5229 1606 Size: 1553 677 1148 245 2120 Fragments arranged by size: 2120 1553 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5191 NdeI CA'TA_TG Cuts at: 387 387 Size: 5743 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5743 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 2917 3101 4646 1132 Size: 97 1688 184 1545 2229 Fragments arranged by size: 2229 1688 1545 184 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5743 PciI A'CATG_T Cuts at: 2913 4642 2913 Size: 1729 4014 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5743 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 5325 5357 3812 Size: 32 1481 32 4198 Fragments arranged by size: 4198 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 3313 3313 Size: 5743 PstI C_TGCA'G Cuts at: 830 2035 2332 2390 2463 3095 830 Size: 1205 297 58 73 632 3478 Fragments arranged by size: 3478 1205 632 297 73 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 SacI G_AGCT'C Cuts at: 2853 2853 Size: 5743 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 3991 5703 3991 Size: 1712 4031 SfcI C'TryA_G Cuts at: 826 1045 2031 2107 2328 2386 2459 3091 4226 Size: 219 986 76 221 58 73 632 1135 Cuts at: 4226 4907 5098 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 986 681 632 221 219 191 76 73 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 2018 3070 1872 Size: 146 1052 4545 Fragments arranged by size: 4545 1052 146 SmlI C'TyrA_G Cuts at: 820 1017 1943 2010 3847 4748 5010 820 Size: 197 926 67 1837 901 262 1553 Fragments arranged by size: 1837 1553 926 901 262 197 67 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 3101 1132 Size: 1969 3774 SspI AAT'ATT Cuts at: 5 52 2789 2945 3667 5 Size: 47 2737 156 722 2081 Fragments arranged by size: 2737 2081 722 156 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5743 StyI C'CwwG_G Cuts at: 513 1065 3182 513 Size: 552 2117 3074 Fragments arranged by size: 3074 2117 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2280 3770 3787 3940 4125 4570 325 Size: 1255 700 1490 17 153 185 445 1498 Fragments arranged by size: 1498 1490 1255 700 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2103 3989 5701 Size: 274 80 33 51 1443 126 1886 1712 Cuts at: 5701 96 Size: 138 Fragments arranged by size: 1886 1712 1443 274 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5703 5735 5703 Size: 32 5711 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5743 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5743 XmaI C'CCGG_G Cuts at: 1870 2016 3068 1870 Size: 146 1052 4545 Fragments arranged by size: 4545 1052 146 XmnI GAAnn'nnTTC Cuts at: 2381 3872 2381 Size: 1491 4252 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3551 276 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI