(Linear) (Six-base) MAPSORT of: KIEE0972.seq Check: 666 from: 1 to: 1560 KIEE0972.orf With 182 enzymes: SgfI * July 15, 2010 18:33 .. AatII G_ACGT'C Cuts at: 0 890 1560 Size: 890 670 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 514 628 1083 1294 1560 Size: 514 114 455 211 266 Fragments arranged by size: 514 455 266 211 114 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 378 1560 Size: 378 1182 AlwNI CAG_nnn'CTG Cuts at: 0 570 1560 Size: 570 990 ApaI G_GGCC'C Cuts at: 0 824 1560 Size: 824 736 AvaI C'yCGr_G Cuts at: 0 35 302 525 901 1069 1294 1560 Size: 35 267 223 376 168 225 266 Fragments arranged by size: 376 267 266 225 223 168 35 BamHI G'GATC_C Cuts at: 0 30 775 1560 Size: 30 745 785 Fragments arranged by size: 785 745 30 BanI G'GyrC_C Cuts at: 0 409 1311 1560 Size: 409 902 249 Fragments arranged by size: 902 409 249 BanII G_rGCy'C Cuts at: 0 824 1220 1259 1361 1508 1560 Size: 824 396 39 102 147 52 Fragments arranged by size: 824 396 147 102 52 39 BbsI GAAGACnn'nnnn_ Cuts at: 0 455 1560 Size: 455 1105 BbvCI CC'TCA_GC Cuts at: 0 665 1474 1560 Size: 665 809 86 Fragments arranged by size: 809 665 86 BglI GCCn_nnn'nGGC Cuts at: 0 1072 1560 Size: 1072 488 BlpI GC'TnA_GC Cuts at: 0 1028 1560 Size: 1028 532 Bme1580I G_kGCm'C Cuts at: 0 824 839 977 1168 1560 Size: 824 15 138 191 392 Fragments arranged by size: 824 392 191 138 15 BmgBI CAC'GTC Cuts at: 0 514 1560 Size: 514 1046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 598 1526 1560 Size: 271 327 928 34 Fragments arranged by size: 928 327 271 34 Bpu10I CC'TnA_GC Cuts at: 0 665 1474 1560 Size: 665 809 86 Fragments arranged by size: 809 665 86 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1243 1560 Size: 1243 317 BsaAI yAC'GTr Cuts at: 0 339 624 782 978 1560 Size: 339 285 158 196 582 Fragments arranged by size: 582 339 285 196 158 BsaBI GATnn'nnATC Cuts at: 0 601 1560 Size: 601 959 BsaHI Gr'CG_yC Cuts at: 0 887 998 1133 1560 Size: 887 111 135 427 Fragments arranged by size: 887 427 135 111 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 571 601 1560 Size: 571 30 959 Fragments arranged by size: 959 571 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 100 874 1267 1369 1387 1390 1420 1423 Size: 100 774 393 102 18 3 30 3 Cuts at: 1423 1560 Size: 137 Fragments arranged by size: 774 393 137 102 100 30 18 3 3 BseYI C'CCAG_C Cuts at: 0 186 262 660 1053 1438 1560 Size: 186 76 398 393 385 122 Fragments arranged by size: 398 393 385 186 122 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 1560 Size: 388 1172 BsiHKAI G_wGCw'C Cuts at: 0 1259 1382 1560 Size: 1259 123 178 Fragments arranged by size: 1259 178 123 BsmI GAATG_Cn' Cuts at: 0 1057 1560 Size: 1057 503 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1291 1560 Size: 1291 269 Bsp1286I G_dGCh'C Cuts at: 0 824 839 977 1168 1220 1259 1361 1382 Size: 824 15 138 191 52 39 102 21 Cuts at: 1382 1508 1560 Size: 126 52 Fragments arranged by size: 824 191 138 126 102 52 52 39 21 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 887 962 1560 Size: 887 75 598 Fragments arranged by size: 887 598 75 BsrBI CCG'CTC Cuts at: 0 231 582 1560 Size: 231 351 978 Fragments arranged by size: 978 351 231 BsrDI GCAATG_nn' Cuts at: 0 470 1560 Size: 470 1090 BssHII G'CGCG_C Cuts at: 0 790 1560 Size: 790 770 BstAPI GCAn_nnn'nTGC Cuts at: 0 570 1560 Size: 570 990 BstEII G'GTnAC_C Cuts at: 0 1338 1560 Size: 1338 222 BstYI r'GATC_y Cuts at: 0 30 775 1560 Size: 30 745 785 Fragments arranged by size: 785 745 30 Bsu36I CC'TnA_GG Cuts at: 0 1232 1560 Size: 1232 328 BtgI C'CryG_G Cuts at: 0 136 275 1220 1448 1560 Size: 136 139 945 228 112 Fragments arranged by size: 945 228 139 136 112 BtsI GCAGTG_nn' Cuts at: 0 912 1380 1560 Size: 912 468 180 Fragments arranged by size: 912 468 180 ClaI AT'CG_AT Cuts at: 0 68 1560 Size: 68 1492 DraIII CAC_nnn'GTG Cuts at: 0 339 1560 Size: 339 1221 DrdI GACnn_nn'nnGTC Cuts at: 0 997 1560 Size: 997 563 EaeI y'GGCC_r Cuts at: 0 232 341 1089 1560 Size: 232 109 748 471 Fragments arranged by size: 748 471 232 109 EarI CTCTTCn'nnn_ Cuts at: 0 1517 1560 Size: 1517 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 327 1560 Size: 327 1233 EcoICRI GAG'CTC Cuts at: 0 1257 1560 Size: 1257 303 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 514 598 628 1083 1294 1526 1560 Size: 271 243 84 30 455 211 232 34 Fragments arranged by size: 455 271 243 232 211 84 34 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 312 814 1560 Size: 312 502 746 Fragments arranged by size: 746 502 312 EcoO109I rG'GnC_Cy Cuts at: 0 280 533 648 820 1263 1560 Size: 280 253 115 172 443 297 Fragments arranged by size: 443 297 280 253 172 115 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1503 1560 Size: 1503 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 760 792 1560 Size: 760 32 768 Fragments arranged by size: 768 760 32 HindIII A'AGCT_T Cuts at: 0 214 1560 Size: 214 1346 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 974 1560 Size: 974 586 MscI TGG'CCA Cuts at: 0 343 1560 Size: 343 1217 MslI CAynn'nnrTG Cuts at: 0 70 626 832 1560 Size: 70 556 206 728 Fragments arranged by size: 728 556 206 70 MspA1I CmG'CkG Cuts at: 0 16 372 567 664 1032 1442 1560 Size: 16 356 195 97 368 410 118 Fragments arranged by size: 410 368 356 195 118 97 16 PasI CC'CwG_GG Cuts at: 0 546 1560 Size: 546 1014 PflMI CCAn_nnn'nTGG Cuts at: 0 339 1560 Size: 339 1221 PfoI T'CCnGG_A Cuts at: 0 650 1542 1560 Size: 650 892 18 Fragments arranged by size: 892 650 18 PmlI CAC'GTG Cuts at: 0 339 624 782 978 1560 Size: 339 285 158 196 582 Fragments arranged by size: 582 339 285 196 158 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1467 1499 1560 Size: 1467 32 61 Fragments arranged by size: 1467 61 32 PpuMI rG'GwC_Cy Cuts at: 0 533 648 1263 1560 Size: 533 115 615 297 Fragments arranged by size: 615 533 297 115 PspOMI G'GGCC_C Cuts at: 0 820 1560 Size: 820 740 PspXI vC'TCGA_Gb Cuts at: 0 901 1560 Size: 901 659 PstI C_TGCA'G Cuts at: 0 392 692 1387 1560 Size: 392 300 695 173 Fragments arranged by size: 695 392 300 173 PvuII CAG'CTG Cuts at: 0 16 372 567 664 1032 1442 1560 Size: 16 356 195 97 368 410 118 Fragments arranged by size: 410 368 356 195 118 97 16 SacI G_AGCT'C Cuts at: 0 1259 1560 Size: 1259 301 SanDI GG'GwC_CC Cuts at: 0 533 1560 Size: 533 1027 SapI GCTCTTCn'nnn_ Cuts at: 0 1517 1560 Size: 1517 43 ScaI AGT'ACT Cuts at: 0 171 907 1560 Size: 171 736 653 Fragments arranged by size: 736 653 171 SfcI C'TryA_G Cuts at: 0 223 388 688 1383 1560 Size: 223 165 300 695 177 Fragments arranged by size: 695 300 223 177 165 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1072 1560 Size: 1072 488 SmaI CCC'GGG Cuts at: 0 304 1560 Size: 304 1256 SmlI C'TyrA_G Cuts at: 0 901 1258 1560 Size: 901 357 302 Fragments arranged by size: 901 357 302 StuI AGG'CCT Cuts at: 0 1111 1560 Size: 1111 449 StyI C'CwwG_G Cuts at: 0 1112 1333 1560 Size: 1112 221 227 Fragments arranged by size: 1112 227 221 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1326 1560 Size: 1326 234 TatI w'GTAC_w Cuts at: 0 169 380 905 1142 1560 Size: 169 211 525 237 418 Fragments arranged by size: 525 418 237 211 169 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 957 1099 1455 1560 Size: 957 142 356 105 Fragments arranged by size: 957 356 142 105 XhoI C'TCGA_G Cuts at: 0 901 1560 Size: 901 659 XmaI C'CCGG_G Cuts at: 0 302 1560 Size: 302 1258 ZraI GAC'GTC Cuts at: 0 888 1560 Size: 888 672 Enzymes that do cut: AatII AcuI AlfI AlwNI ApaI AvaI BamHI BanI BanII BbsI BbvCI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BssHII BstAPI BstEII BstYI Bsu36I BtgI BtsI ClaI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HindIII MmeI MscI MslI MspA1I PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SanDI SapI ScaI SfcI SfiI SmaI SmlI StuI StyI TaqII TatI XcmI XhoI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglII BmrI BmtI BplI BsaI BsaWI BsiEI BsiWI BspEI BspHI BsrFI BsrGI BssSI BstBI BstXI BstZ17I BtgZI CspCI DraI EagI EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PshAI PsiI PsrI PvuI RsrII SacII SalI SbfI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XmnI