################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI BstYI Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MroI MseI MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I TthHB8I XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 215 GTATAC GT^[AC][GT]AC AccII 739 CGCG CG^CG AcsI 242 GAATTC [GA]^AATT[CT] AlwNI 407 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 242 GAATTC [GA]^AATT[CT] AspHI 442 GTGCTC G[AT]GC[AT]^C AspI 817 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 562 CCCGAG C^[CT]CG[GA]G AvaII 161 GGTCC G^G[AT]CC BbsI 518 GTCTTC GAAGAC(2/6) BfaI 805 CTAG C^TAG BpuAI 518 GTCTTC GAAGAC(2/6) BseRI 184 GAGGAG GAGGAG(10/8) BsgI 313 CTGCAC GTGCAG(16/14) BsiHKAI 442 GTGCTC G[AT]GC[AT]^C BsoBI 562 CCCGAG C^[CT]CG[GA]G BspMI 496 ACCTGC ACCTGC(4/8) BsrDI 179 GCAATG GCAATG(2/0) Bst1107I 215 GTATAC GTA^TAC BstUI 739 CGCG CG^CG BstZ17I 215 GTATAC GTA^TAC EcoNI 158 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 242 GAATTC G^AATTC EcoT14I 579 CCATGG C^C[AT][AT]GG FauI 666 GCGGG CCCGC(4/6) HindIII 336 AAGCTT A^AGCTT MaeI 805 CTAG C^TAG MaeII 504 ACGT A^CGT MslI 460 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 739 CGCG CG^CG NcoI 579 CCATGG C^CATGG PflFI 817 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 406 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 111 GACTC GAGTC(4/5) PstI 607 CTGCAG CTGCA^G PvuII 770 CAGCTG CAG^CTG SfcI 607 CTGCAG C^T[GA][CT]AG SmlI 13 CTCAAG C^T[CT][GA]AG StuI 566 AGGCCT AGG^CCT StyI 579 CCATGG C^C[AT][AT]GG Tth111I 817 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 406 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 804 TCTAGA T^CTAGA XcmI 640 CCAAAGCCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 44 GGATC GGATC(4/5) 361 GGATC BalI 422 TGGCCA TGG^CCA 600 TGGCCA BmyI 442 GTGCTC G[AGT]GC[ACT]^C 687 GGGCAC BpmI 544 CTCCAG CTGGAG(16/14) 748 CTGGAG BsaI 84 GGTCTC GGTCTC(1/5) 327 GGTCTC Bsp1286I 442 GTGCTC G[AGT]GC[ACT]^C 687 GGGCAC BstF5I 50 CATCC GGATG(2/0) 662 GGATG CfoI 740 GCGC GCG^C 904 GCGC DpnII 45 GATC ^GATC 362 GATC DsaI 579 CCATGG C^C[GA][CT]GG 848 CCGTGG FokI 50 CATCC GGATG(9/13) 662 GGATG HhaI 740 GCGC GCG^C 904 GCGC HinP1I 740 GCGC G^CGC 904 GCGC MaeIII 295 GTGAC ^GT[ACGT]AC 491 GTGAC MboI 45 GATC ^GATC 362 GATC MluNI 422 TGGCCA TGG^CCA 600 TGGCCA MscI 422 TGGCCA TGG^CCA 600 TGGCCA NdeII 45 GATC ^GATC 362 GATC NlaIV 18 GGAGCC GG[ACGT]^[ACGT]CC 583 GGCCCC Sau3AI 45 GATC ^GATC 362 GATC TfiI 453 GAATC G^A[AT]TC 632 GATTC Tsp45I 295 GTGAC ^GT[GC]AC 491 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI BsmAI BsmFI BsrI BssKI BstNI Cfr13I DdeI EaeI EcoRII Fnu4HI HaeIII HinfI HphI ItaI MboII MnlI MspA1I MvaI MwoI NlaIII Sau96I ScrFI SfaNI TseI Tsp509I TspRI