################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbrPI BbsI BcgI BciVI BclI BfrI BglII BlnI BpuAI BsaAI BsaBI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI DraI DraIII Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI MaeII MamI MfeI MluI MluNI MscI MseI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SalI SapI SbfI ScaI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccIII 881 TCCGGA T^CCGGA AfaI 84 GTAC GT^AC AocI 664 CCTGAGG CC^T[ACGT]AGG AscI 126 GGCGCGCC GG^CGCGCC AspHI 187 GAGCTC G[AT]GC[AT]^C BamHI 965 GGATCC G^GATCC BbvCI 1184 CCTCAGC CCTCAGC(-5/-2) BglI 1192 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 466 GCTGAGC GC^T[ACGT]AGC Bpu1102I 466 GCTGAGC GC^T[ACGT]AGC BsaWI 881 TCCGGA [AT]^CCGG[AT] BseAI 881 TCCGGA T^CCGGA BsgI 1085 CTGCAC GTGCAG(16/14) BsiHKAI 187 GAGCTC G[AT]GC[AT]^C BspEI 881 TCCGGA T^CCGGA BsrBI 760 GAGCGG CCGCTC(-3/-3) BsrI 804 ACTGG ACTGG(1/-1) BstAPI 255 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 664 CCTGAGG CC^T[ACGT]AGG CelII 466 GCTGAGC GC^T[ACGT]AGC CpoI 359 CGGACCG CG^G[AT]CCG DrdI 835 GACCACCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1108 GAAGAG CTCTTC(1/4) Eco81I 664 CCTGAGG CC^T[ACGT]AGG EcoNI 789 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 961 CCATGG C^C[AT][AT]GG HphI 459 GGTGA GGTGA(8/7) Ksp632I 1108 GAAGAG CTCTTC(1/4) MaeIII 355 GTCAC ^GT[ACGT]AC MroI 881 TCCGGA T^CCGGA MslI 867 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 961 CCATGG C^CATGG PaeR7I 859 CTCGAG C^TCGAG RsaI 84 GTAC GT^AC RsrII 359 CGGACCG CG^G[AT]CCG SacI 187 GAGCTC GAGCT^C SfaNI 64 GCATC GCATC(5/9) SgrAI 639 CGCCGGCG C[GA]^CCGG[CT]G StyI 961 CCATGG C^C[AT][AT]GG TaqI 860 TCGA T^CGA TfiI 1057 GAATC G^A[AT]TC Tsp45I 355 GTCAC ^GT[GC]AC TthHB8I 860 TCGA T^CGA XhoI 859 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### ApaI 98 GGGCCC GGGCC^C 211 GGGCCC BpmI 1130 CTCCAG CTGGAG(16/14) 184 CTGGAG BspMI 398 ACCTGC ACCTGC(4/8) 231 GCAGGT BsrFI 246 GCCGGC [GA]^CCGG[CT] 640 GCCGGC BstXI 537 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 866 CCATGAAGGTGG Cfr10I 246 GCCGGC [GA]^CCGG[CT] 640 GCCGGC EagI 68 CGGCCG C^GGCCG 152 CGGCCG EclXI 68 CGGCCG C^GGCCG 152 CGGCCG Eco52I 68 CGGCCG C^GGCCG 152 CGGCCG KspI 53 CCGCGG CCGC^GG 71 CCGCGG NaeI 246 GCCGGC GCC^GGC 640 GCCGGC NgoMIV 246 GCCGGC G^CCGGC 640 GCCGGC PpuMI 668 AGGACCC [GA]G^G[AT]CC[CT] 842 GGGTCCC PvuII 439 CAGCTG CAG^CTG 502 CAGCTG SacII 53 CCGCGG CCGC^GG 71 CCGCGG SmaI 22 CCCGGG CCC^GGG 601 CCCGGG XmaCI 22 CCCGGG C^CCGGG 601 CCCGGG XmaI 22 CCCGGG C^CCGGG 601 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AlwNI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BmyI BsaHI BsaI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI KasI MaeI MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfcI SfoI SmlI TseI TspRI XhoII