(Circular) (Six-base) MAPSORT of: pFN21AE1494.seq Check: 9976 from: 1 to: 5446 pFN21AE1494 5446 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3256 278 Size: 53 83 186 936 1720 2468 Fragments arranged by size: 2468 1720 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2789 1937 Size: 852 4594 Acc65I G'GTAC_C Cuts at: 2767 2767 Size: 5446 AclI AA'CG_TT Cuts at: 1563 3573 3946 1563 Size: 2010 373 3063 Fragments arranged by size: 3063 2010 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2162 3509 4893 5161 1632 Size: 530 1347 1384 268 1917 Fragments arranged by size: 1917 1384 1347 530 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5249 AflIII A'CryG_T Cuts at: 1203 4345 5322 1203 Size: 3142 977 1327 Fragments arranged by size: 3142 1327 977 AgeI A'CCGG_T Cuts at: 4277 4277 Size: 5446 AhdI GACnn_n'nnGTC Cuts at: 4175 4175 Size: 5446 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5446 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5446 AlwNI CAG_nnn'CTG Cuts at: 3189 4761 3189 Size: 1572 3874 ApaI G_GGCC'C Cuts at: 2233 2233 Size: 5446 ApaLI G'TGCA_C Cuts at: 3503 4659 3503 Size: 1156 4290 ApoI r'AATT_y Cuts at: 1493 2755 2981 3133 5242 1493 Size: 1262 226 152 2109 1697 Fragments arranged by size: 2109 1697 1262 226 152 AseI AT'TA_AT Cuts at: 160 1032 4000 160 Size: 872 2968 1606 Fragments arranged by size: 2968 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5446 AvaI C'yCGr_G Cuts at: 1870 1943 2771 1870 Size: 73 828 4545 Fragments arranged by size: 4545 828 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2536 2569 2536 Size: 33 5413 BamHI G'GATC_C Cuts at: 2776 3143 2776 Size: 367 5079 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2767 4222 618 Size: 325 217 627 980 1455 1842 Fragments arranged by size: 1842 1455 980 627 325 217 BanII G_rGCy'C Cuts at: 1391 2233 2765 1391 Size: 842 532 4072 Fragments arranged by size: 4072 842 532 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4475 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2495 2529 3637 3671 1903 Size: 34 50 34 474 34 1108 34 3678 Fragments arranged by size: 3678 1108 474 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3338 4548 3338 Size: 1210 4236 BclI T'GATC_A Cuts at: 1548 1548 Size: 5446 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2386 4057 136 Size: 107 122 71 631 605 714 1671 1525 Fragments arranged by size: 1671 1525 714 631 605 122 107 71 BglII A'GATC_T Cuts at: 5441 5441 Size: 5446 BlpI GC'TnA_GC Cuts at: 2863 2863 Size: 5446 Bme1580I G_kGCm'C Cuts at: 1790 2233 3507 4663 1790 Size: 443 1274 1156 2573 Fragments arranged by size: 2573 1274 1156 443 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5446 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3177 4135 442 Size: 416 53 83 399 24 1760 958 1753 Fragments arranged by size: 1760 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5446 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2366 2634 4106 1239 Size: 1127 268 1472 2579 Fragments arranged by size: 2579 1472 1127 268 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2689 3571 4436 4734 2689 Size: 882 865 298 3401 Fragments arranged by size: 3401 882 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4109 882 Size: 617 2610 2219 Fragments arranged by size: 2610 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3237 493 Size: 711 2033 2702 Fragments arranged by size: 2702 2033 711 BsaBI GATnn'nnATC Cuts at: 5440 5440 Size: 5446 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3253 3635 4270 275 Size: 53 83 186 936 1720 382 635 1451 Fragments arranged by size: 1720 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3879 4277 4551 4698 5109 3879 Size: 398 274 147 411 4216 Fragments arranged by size: 4216 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5221 Fragments arranged by size: 5221 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5446 BseYI C'CCAG_C Cuts at: 2259 2442 4649 5145 2259 Size: 183 2207 496 2560 Fragments arranged by size: 2560 2207 496 183 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2396 2525 2677 1163 Size: 573 660 129 152 3932 Fragments arranged by size: 3932 660 573 152 129 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2896 3657 4685 1540 Size: 275 186 895 761 1028 2301 Fragments arranged by size: 2301 1028 895 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3507 3592 4663 3507 Size: 85 1071 4290 Fragments arranged by size: 4290 1071 85 BsmI GAATG_Cn' Cuts at: 2957 3050 2957 Size: 93 5353 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2233 2765 3507 3592 4663 1391 Size: 399 443 532 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 532 443 399 85 BspEI T'CCGG_A Cuts at: 5109 5109 Size: 5446 BspHI T'CATG_A Cuts at: 3335 5065 3335 Size: 1730 3716 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2801 844 Size: 1957 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3333 1394 Size: 429 1510 3507 Fragments arranged by size: 3507 1510 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3941 4115 65 Size: 1183 2693 174 1396 Fragments arranged by size: 2693 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2438 4090 4277 1700 Size: 110 628 1652 187 2869 Fragments arranged by size: 2869 1652 628 187 110 BsrGI T'GTAC_A Cuts at: 96 2713 96 Size: 2617 2829 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5446 BssSI C'ACGA_G Cuts at: 2685 3506 4518 2685 Size: 821 1012 3613 Fragments arranged by size: 3613 1012 821 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5446 BstBI TT'CG_AA Cuts at: 4250 4250 Size: 5446 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5446 BstYI r'GATC_y Cuts at: 1972 2776 3143 3528 3545 4997 5083 5095 5441 Size: 804 367 385 17 1452 86 12 346 Cuts at: 5441 1972 Size: 1977 Fragments arranged by size: 1977 1452 804 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2384 2384 Size: 5446 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4894 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4707 Fragments arranged by size: 4707 683 56 BtsI GCAGTG_nn' Cuts at: 2437 2962 3756 3776 2437 Size: 525 794 20 4107 Fragments arranged by size: 4107 794 525 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5446 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5411 DraI TTT'AAA Cuts at: 2750 2750 Size: 5446 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4453 809 Size: 507 3137 1802 Fragments arranged by size: 3137 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2436 2893 3782 Size: 54 1425 50 275 18 606 457 889 Cuts at: 3782 5411 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 606 457 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2893 1537 Size: 275 1081 4090 Fragments arranged by size: 4090 1081 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2138 3376 1353 Size: 785 1238 3423 Fragments arranged by size: 3423 1238 785 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4033 4407 4553 387 Size: 1064 138 2444 374 146 1280 Fragments arranged by size: 2444 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2366 2634 3509 4106 4893 5161 Size: 393 530 204 268 875 597 787 268 Cuts at: 5161 1239 Size: 1524 Fragments arranged by size: 1524 875 787 597 530 393 268 268 204 EcoO109I rG'GnC_Cy Cuts at: 2229 2575 2618 2229 Size: 346 43 5057 Fragments arranged by size: 5057 346 43 EcoRI G'AATT_C Cuts at: 2755 3133 2755 Size: 378 5068 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2614 2614 Size: 5446 FspI TGC'GCA Cuts at: 3952 3952 Size: 5446 HaeII r_GCGC'y Cuts at: 1678 4593 1678 Size: 2915 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2113 2120 2145 2152 2176 Size: 32 135 32 541 7 25 7 24 Cuts at: 2176 2208 4087 4119 4161 4193 1373 Size: 32 1879 32 42 32 2626 Fragments arranged by size: 2626 1879 541 135 42 32 32 32 32 32 25 24 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2790 3036 669 Size: 988 281 852 246 3079 Fragments arranged by size: 3079 988 852 281 246 HindIII A'AGCT_T Cuts at: 748 2707 748 Size: 1959 3487 HpaI GTT'AAC Cuts at: 3036 3036 Size: 5446 KpnI G_GTAC'C Cuts at: 2771 2771 Size: 5446 MfeI C'AATT_G Cuts at: 3045 3045 Size: 5446 MluI A'CGCG_T Cuts at: 5322 5322 Size: 5446 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2346 4560 4744 1305 Size: 1041 2214 184 2007 Fragments arranged by size: 2214 2007 1041 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3624 Fragments arranged by size: 3624 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3404 3763 3922 518 Size: 648 2238 359 159 2042 Fragments arranged by size: 2238 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2189 2344 2862 3539 4687 4932 1606 Size: 583 155 518 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 583 518 245 155 NaeI GCC'GGC Cuts at: 2440 2440 Size: 5446 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4894 NdeI CA'TA_TG Cuts at: 387 387 Size: 5446 NgoMIV G'CCGG_C Cuts at: 2438 2438 Size: 5446 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5446 NotI GC'GGCC_GC Cuts at: 2893 2893 Size: 5446 NspI r_CATG'y Cuts at: 1132 1229 2430 2680 2804 4349 1132 Size: 97 1201 250 124 1545 2229 Fragments arranged by size: 2229 1545 1201 250 124 97 PasI CC'CwG_GG Cuts at: 1348 2046 2461 2644 1348 Size: 698 415 183 4150 Fragments arranged by size: 4150 698 415 183 PciI A'CATG_T Cuts at: 4345 4345 Size: 5446 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5446 PmeI GTTT'AAAC Cuts at: 2750 2750 Size: 5446 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3515 3547 5028 5060 3515 Size: 32 1481 32 3901 Fragments arranged by size: 3901 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2575 2575 Size: 5446 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5446 PsiI TTA'TAA Cuts at: 3016 3016 Size: 5446 PspOMI G'GGCC_C Cuts at: 2229 2229 Size: 5446 PstI C_TGCA'G Cuts at: 830 2343 2798 830 Size: 1513 455 3478 Fragments arranged by size: 3478 1513 455 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5446 PvuII CAG'CTG Cuts at: 2189 2344 2189 Size: 155 5291 SalI G'TCGA_C Cuts at: 1936 2788 1936 Size: 852 4594 SanDI GG'GwC_CC Cuts at: 2575 2575 Size: 5446 SbfI CC_TGCA'GG Cuts at: 2798 2798 Size: 5446 ScaI AGT'ACT Cuts at: 3694 5406 3694 Size: 1712 3734 SfcI C'TryA_G Cuts at: 826 1045 2339 2794 3929 4610 4801 826 Size: 219 1294 455 1135 681 191 1471 Fragments arranged by size: 1471 1294 1135 681 455 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5446 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5446 SmaI CCC'GGG Cuts at: 1872 2773 1872 Size: 901 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2704 3550 4451 4713 820 Size: 197 926 761 846 901 262 1553 Fragments arranged by size: 1553 926 901 846 761 262 197 SnaBI TAC'GTA Cuts at: 493 3237 493 Size: 2744 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5446 SphI G_CATG'C Cuts at: 1132 2680 2804 1132 Size: 1548 124 3774 Fragments arranged by size: 3774 1548 124 SspI AAT'ATT Cuts at: 5 52 3370 5 Size: 47 3318 2081 Fragments arranged by size: 3318 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5446 StyI C'CwwG_G Cuts at: 513 1065 2223 2885 513 Size: 552 1158 662 3074 Fragments arranged by size: 3074 1158 662 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3473 3490 3643 3828 4273 325 Size: 1255 1893 17 153 185 445 1498 Fragments arranged by size: 1893 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2713 3692 5404 Size: 274 80 33 51 1443 736 979 1712 Cuts at: 5404 96 Size: 138 Fragments arranged by size: 1712 1443 979 736 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5406 5438 5406 Size: 32 5414 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5446 XbaI T'CTAG_A Cuts at: 2782 2782 Size: 5446 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5446 XmaI C'CCGG_G Cuts at: 1870 2771 1870 Size: 901 4545 XmnI GAAnn'nnTTC Cuts at: 3575 3575 Size: 5446 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3254 276 Size: 53 83 186 936 1720 2468 Fragments arranged by size: 2468 1720 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI