(Linear) (Six-base) MAPSORT of: KIEE3947.seq Check: 1791 from: 1 to: 3294 KIEE3947.orf With 182 enzymes: SgfI * March 22, 2012 16:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1906 1946 3294 Size: 1906 40 1348 Fragments arranged by size: 1906 1348 40 AccI GT'mk_AC Cuts at: 0 3206 3294 Size: 3206 88 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1403 2876 3294 Size: 1403 1473 418 Fragments arranged by size: 1473 1403 418 AfeI AGC'GCT Cuts at: 0 2682 3294 Size: 2682 612 AflIII A'CryG_T Cuts at: 0 2445 3029 3294 Size: 2445 584 265 Fragments arranged by size: 2445 584 265 AlwNI CAG_nnn'CTG Cuts at: 0 238 1557 2208 3294 Size: 238 1319 651 1086 Fragments arranged by size: 1319 1086 651 238 ApaLI G'TGCA_C Cuts at: 0 1076 3294 Size: 1076 2218 ApoI r'AATT_y Cuts at: 0 390 815 1114 2255 2625 3294 Size: 390 425 299 1141 370 669 Fragments arranged by size: 1141 669 425 390 370 299 AseI AT'TA_AT Cuts at: 0 1477 2495 3294 Size: 1477 1018 799 Fragments arranged by size: 1477 1018 799 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2794 2827 3294 Size: 2794 33 467 Fragments arranged by size: 2794 467 33 BamHI G'GATC_C Cuts at: 0 113 3294 Size: 113 3181 BanI G'GyrC_C Cuts at: 0 2010 3294 Size: 2010 1284 BanII G_rGCy'C Cuts at: 0 2193 2249 3294 Size: 2193 56 1045 Fragments arranged by size: 2193 1045 56 BbsI GAAGACnn'nnnn_ Cuts at: 0 806 3294 Size: 806 2488 BciVI GTATCCnnnnn_n' Cuts at: 0 927 3294 Size: 927 2367 BfrBI ATG'CAT Cuts at: 0 603 3294 Size: 603 2691 BglII A'GATC_T Cuts at: 0 12 196 1805 2322 3294 Size: 12 184 1609 517 972 Fragments arranged by size: 1609 972 517 184 12 BlpI GC'TnA_GC Cuts at: 0 251 3294 Size: 251 3043 Bme1580I G_kGCm'C Cuts at: 0 1080 1596 3294 Size: 1080 516 1698 Fragments arranged by size: 1698 1080 516 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 125 221 2639 3294 Size: 125 96 2418 655 Fragments arranged by size: 2418 655 125 96 Bpu10I CC'TnA_GC Cuts at: 0 1469 1753 2248 3294 Size: 1469 284 495 1046 Fragments arranged by size: 1469 1046 495 284 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 594 3294 Size: 594 2700 BsaAI yAC'GTr Cuts at: 0 2050 3294 Size: 2050 1244 BsaHI Gr'CG_yC Cuts at: 0 294 577 1580 1949 3294 Size: 294 283 1003 369 1345 Fragments arranged by size: 1345 1003 369 294 283 BsaWI w'CCGG_w Cuts at: 0 3191 3294 Size: 3191 103 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2 32 62 92 3294 Size: 2 30 30 30 3202 Fragments arranged by size: 3202 30 30 30 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1588 3115 3294 Size: 1588 1527 179 Fragments arranged by size: 1588 1527 179 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 213 3178 3294 Size: 213 2965 116 Fragments arranged by size: 2965 213 116 BsiHKAI G_wGCw'C Cuts at: 0 1080 2249 3294 Size: 1080 1169 1045 Fragments arranged by size: 1169 1080 1045 BsmI GAATG_Cn' Cuts at: 0 511 3294 Size: 511 2783 Bsp1286I G_dGCh'C Cuts at: 0 1080 1596 2193 2249 3294 Size: 1080 516 597 56 1045 Fragments arranged by size: 1080 1045 597 516 56 BspHI T'CATG_A Cuts at: 0 3249 3294 Size: 3249 45 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 220 1049 1296 1906 1932 1946 3294 Size: 220 829 247 610 26 14 1348 Fragments arranged by size: 1348 829 610 247 220 26 14 BsrDI GCAATG_nn' Cuts at: 0 1219 1319 2070 2136 3294 Size: 1219 100 751 66 1158 Fragments arranged by size: 1219 1158 751 100 66 BsrGI T'GTAC_A Cuts at: 0 1103 1955 3294 Size: 1103 852 1339 Fragments arranged by size: 1339 1103 852 BstAPI GCAn_nnn'nTGC Cuts at: 0 247 2925 3294 Size: 247 2678 369 Fragments arranged by size: 2678 369 247 BstBI TT'CG_AA Cuts at: 0 3120 3294 Size: 3120 174 BstXI CCAn_nnnn'nTGG Cuts at: 0 2955 3294 Size: 2955 339 BstYI r'GATC_y Cuts at: 0 12 94 113 196 1805 2322 3294 Size: 12 82 19 83 1609 517 972 Fragments arranged by size: 1609 972 517 83 82 19 12 Bsu36I CC'TnA_GG Cuts at: 0 1121 1866 2297 3294 Size: 1121 745 431 997 Fragments arranged by size: 1121 997 745 431 BtgI C'CryG_G Cuts at: 0 221 2663 3294 Size: 221 2442 631 Fragments arranged by size: 2442 631 221 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1772 2470 3294 Size: 1772 698 824 Fragments arranged by size: 1772 824 698 BtsI GCAGTG_nn' Cuts at: 0 179 876 1820 2366 2879 3294 Size: 179 697 944 546 513 415 Fragments arranged by size: 944 697 546 513 415 179 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1412 1447 3294 Size: 1412 35 1847 Fragments arranged by size: 1847 1412 35 DraI TTT'AAA Cuts at: 0 644 1023 2933 3294 Size: 644 379 1910 361 Fragments arranged by size: 1910 644 379 361 DraIII CAC_nnn'GTG Cuts at: 0 1264 2925 3294 Size: 1264 1661 369 Fragments arranged by size: 1661 1264 369 EaeI y'GGCC_r Cuts at: 0 876 1559 3294 Size: 876 683 1735 Fragments arranged by size: 1735 876 683 EarI CTCTTCn'nnn_ Cuts at: 0 1607 2137 2822 2852 3294 Size: 1607 530 685 30 442 Fragments arranged by size: 1607 685 530 442 30 EcoICRI GAG'CTC Cuts at: 0 2247 3294 Size: 2247 1047 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 125 221 1403 2639 2876 3294 Size: 125 96 1182 1236 237 418 Fragments arranged by size: 1236 1182 418 237 125 96 EcoRV GAT'ATC Cuts at: 0 761 1275 1881 3294 Size: 761 514 606 1413 Fragments arranged by size: 1413 761 606 514 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 820 904 3063 3294 Size: 820 84 2159 231 Fragments arranged by size: 2159 820 231 84 HaeII r_GCGC'y Cuts at: 0 2684 2865 3294 Size: 2684 181 429 Fragments arranged by size: 2684 429 181 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 79 98 111 130 2150 2182 3244 3276 Size: 79 19 13 19 2020 32 1062 32 Cuts at: 3276 3294 Size: 18 Fragments arranged by size: 2020 1062 79 32 32 19 19 18 13 HincII GTy'rAC Cuts at: 0 1641 1947 3000 3294 Size: 1641 306 1053 294 Fragments arranged by size: 1641 1053 306 294 HindIII A'AGCT_T Cuts at: 0 766 1732 3294 Size: 766 966 1562 Fragments arranged by size: 1562 966 766 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 429 1024 1402 2792 3294 Size: 429 595 378 1390 502 Fragments arranged by size: 1390 595 502 429 378 MscI TGG'CCA Cuts at: 0 878 3294 Size: 878 2416 MslI CAynn'nnrTG Cuts at: 0 56 526 746 2602 2741 2743 2872 3294 Size: 56 470 220 1856 139 2 129 422 Fragments arranged by size: 1856 470 422 220 139 129 56 2 NcoI C'CATG_G Cuts at: 0 221 2663 3294 Size: 221 2442 631 Fragments arranged by size: 2442 631 221 NsiI A_TGCA'T Cuts at: 0 605 3294 Size: 605 2689 NspI r_CATG'y Cuts at: 0 541 2346 2449 2601 3033 3294 Size: 541 1805 103 152 432 261 Fragments arranged by size: 1805 541 432 261 152 103 PciI A'CATG_T Cuts at: 0 2445 3029 3294 Size: 2445 584 265 Fragments arranged by size: 2445 584 265 PflMI CCAn_nnn'nTGG Cuts at: 0 964 3294 Size: 964 2330 PfoI T'CCnGG_A Cuts at: 0 2207 3294 Size: 2207 1087 PsiI TTA'TAA Cuts at: 0 695 3294 Size: 695 2599 PstI C_TGCA'G Cuts at: 0 47 1943 3294 Size: 47 1896 1351 Fragments arranged by size: 1896 1351 47 SacI G_AGCT'C Cuts at: 0 2249 3294 Size: 2249 1045 SapI GCTCTTCn'nnn_ Cuts at: 0 2852 3294 Size: 2852 442 SbfI CC_TGCA'GG Cuts at: 0 1943 3294 Size: 1943 1351 SexAI A'CCwGG_T Cuts at: 0 963 3294 Size: 963 2331 SfcI C'TryA_G Cuts at: 0 43 1939 2400 3294 Size: 43 1896 461 894 Fragments arranged by size: 1896 894 461 43 SmlI C'TyrA_G Cuts at: 0 609 3294 Size: 609 2685 SnaBI TAC'GTA Cuts at: 0 2050 3294 Size: 2050 1244 SpeI A'CTAG_T Cuts at: 0 190 3294 Size: 190 3104 SspI AAT'ATT Cuts at: 0 1501 1980 3294 Size: 1501 479 1314 Fragments arranged by size: 1501 1314 479 StyI C'CwwG_G Cuts at: 0 81 221 2663 2791 3184 3294 Size: 81 140 2442 128 393 110 Fragments arranged by size: 2442 393 140 128 110 81 SwaI ATTT'AAAT Cuts at: 0 644 3294 Size: 644 2650 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2783 3294 Size: 2783 511 TatI w'GTAC_w Cuts at: 0 89 1103 1955 3012 3294 Size: 89 1014 852 1057 282 Fragments arranged by size: 1057 1014 852 282 89 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2696 2728 2899 2931 3294 Size: 2696 32 171 32 363 Fragments arranged by size: 2696 363 171 32 32 Tth111I GACn'n_nGTC Cuts at: 0 546 3294 Size: 546 2748 XbaI T'CTAG_A Cuts at: 0 342 3294 Size: 342 2952 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AlwNI ApaLI ApoI AseI BaeI BamHI BanI BanII BbsI BciVI BfrBI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoRV FalI HaeII Hin4I HincII HindIII MmeI MscI MslI NcoI NsiI NspI PciI PflMI PfoI PsiI PstI SacI SapI SbfI SexAI SfcI SmlI SnaBI SpeI SspI StyI SwaI TaqII TatI TstI Tth111I XbaI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaI AscI AsiSI AvaI AvrII BbeI BbvCI BcgI BclI BglI BmgBI BmrI BmtI BplI BsaI BsaBI BseYI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstEII BstZ17I ClaI DrdI EagI EciI EcoNI EcoO109I EcoRI FseI FspI FspAI HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI ScaI SfiI SfoI SgfI SgrAI SmaI SphI SrfI StuI XcmI XhoI XmaI XmnI ZraI