Restriction map for
pFN21AE6353
(Circular) (Six-base) MAPSORT of: pFN21AE6353.seq Check: 538 from: 1 to: 5869
pFN21AE6353 5869 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3679 278
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3212 1937
Size: 1275 4594
Acc65I G'GTAC_C
Cuts at: 2888 3190 2888
Size: 302 5567
AclI AA'CG_TT
Cuts at: 1563 3996 4369 1563
Size: 2433 373 3063
Fragments arranged by size:
3063 2433 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2688 3060 3075 3932 5316 5584 1632
Size: 1056 372 15 857 1384 268 1917
Fragments arranged by size:
1917 1384 1056 857 372 268 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5672
AflIII A'CryG_T
Cuts at: 1203 4768 5745 1203
Size: 3565 977 1327
Fragments arranged by size:
3565 1327 977
AgeI A'CCGG_T
Cuts at: 4700 4700
Size: 5869
AhdI GACnn_n'nnGTC
Cuts at: 4598 4598
Size: 5869
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5869
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2250 1184
Size: 1066 4803
AlwNI CAG_nnn'CTG
Cuts at: 2024 3142 3612 5184 2024
Size: 1118 470 1572 2709
Fragments arranged by size:
2709 1572 1118 470
ApaI G_GGCC'C
Cuts at: 2613 2980 2613
Size: 367 5502
ApaLI G'TGCA_C
Cuts at: 2714 3926 5082 2714
Size: 1212 1156 3501
Fragments arranged by size:
3501 1212 1156
ApoI r'AATT_y
Cuts at: 1493 3178 3404 3556 5665 1493
Size: 1685 226 152 2109 1697
Fragments arranged by size:
2109 1697 1685 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4423 160
Size: 872 3391 1606
Fragments arranged by size:
3391 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 3194 1870
Size: 73 115 1136 4545
Fragments arranged by size:
4545 1136 115 73
BamHI G'GATC_C
Cuts at: 2840 3199 3566 2840
Size: 359 367 5143
Fragments arranged by size:
5143 367 359
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2418 2537 2888 2938
Size: 325 217 627 285 346 119 351 50
Cuts at: 2938 3190 4645 618
Size: 252 1455 1842
Fragments arranged by size:
1842 1455 627 351 346 325 285 252
217 119 50
BanII G_rGCy'C
Cuts at: 1391 2013 2057 2115 2124 2613 2676 2980 3188
Size: 622 44 58 9 489 63 304 208
Cuts at: 3188 1391
Size: 4072
Fragments arranged by size:
4072 622 489 304 208 63 58 44
9
BbeI G_GCGC'C
Cuts at: 2076 2076
Size: 5869
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2251 928
Size: 971 352 4546
Fragments arranged by size:
4546 971 352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2519 2553 4060 4094 1903
Size: 34 582 34 1507 34 3678
Fragments arranged by size:
3678 1507 582 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2917 3761 4971 2917
Size: 844 1210 3815
Fragments arranged by size:
3815 1210 844
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5869
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3050 4480 136
Size: 107 122 71 631 605 1378 1430 1525
Fragments arranged by size:
1525 1430 1378 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5864 5864
Size: 5869
BlpI GC'TnA_GC
Cuts at: 3286 3286
Size: 5869
Bme1580I G_kGCm'C
Cuts at: 1790 2613 2718 2836 2980 3930 5086 1790
Size: 823 105 118 144 950 1156 2573
Fragments arranged by size:
2573 1156 950 823 144 118 105
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5869
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3130 3600 4558
Size: 416 53 83 399 24 1713 470 958
Cuts at: 4558 442
Size: 1753
Fragments arranged by size:
1753 1713 958 470 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2001 2675 3076 4529 1239
Size: 762 674 401 1453 2579
Fragments arranged by size:
2579 1453 762 674 401
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3994 4859 5157 3994
Size: 865 298 4706
Fragments arranged by size:
4706 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2030 2461 4532 882
Size: 617 531 431 2071 2219
Fragments arranged by size:
2219 2071 617 531 431
BsaAI yAC'GTr
Cuts at: 493 1204 2719 3660 493
Size: 711 1515 941 2702
Fragments arranged by size:
2702 1515 941 711
BsaBI GATnn'nnATC
Cuts at: 5863 5863
Size: 5869
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 3676 4058 4693
Size: 53 83 186 936 540 1603 382 635
Cuts at: 4693 275
Size: 1451
Fragments arranged by size:
1603 1451 936 635 540 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4302 4700 4974 5121 5532 4302
Size: 398 274 147 411 4639
Fragments arranged by size:
4639 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5644
Fragments arranged by size:
5644 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 2672 1186
Size: 961 525 4383
Fragments arranged by size:
4383 961 525
BseYI C'CCAG_C
Cuts at: 2024 2267 2606 2722 5072 5568 2024
Size: 243 339 116 2350 496 2325
Fragments arranged by size:
2350 2325 496 339 243 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2495 2582 2951 2991 3082 1163
Size: 573 759 87 369 40 91 3950
Fragments arranged by size:
3950 759 573 369 91 87 40
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3319 4080 5108 1540
Size: 275 186 1318 761 1028 2301
Fragments arranged by size:
2301 1318 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2718 3930 4015 5086 2718
Size: 1212 85 1071 3501
Fragments arranged by size:
3501 1212 1071 85
BsmI GAATG_Cn'
Cuts at: 3380 3473 3380
Size: 93 5776
BsmBI CGTCTCn'nnnn_
Cuts at: 2856 2856
Size: 5869
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2057 2115 2124 2613 2676 2718
Size: 399 223 44 58 9 489 63 42
Cuts at: 2718 2836 2980 3188 3930 4015 5086 1391
Size: 118 144 208 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 489 399 223 208 144
118 85 63 58 44 42 9
BspEI T'CCGG_A
Cuts at: 5532 5532
Size: 5869
BspHI T'CATG_A
Cuts at: 2310 3758 5488 2310
Size: 1448 1730 2691
Fragments arranged by size:
2691 1730 1448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3097 3224 844
Size: 2253 127 3489
Fragments arranged by size:
3489 2253 127
BsrBI CCG'CTC
Cuts at: 1394 1823 2849 3756 1394
Size: 429 1026 907 3507
Fragments arranged by size:
3507 1026 907 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2905 4364 4538 65
Size: 1183 1657 1459 174 1396
Fragments arranged by size:
1657 1459 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4513 4700 1700
Size: 110 2703 187 2869
Fragments arranged by size:
2869 2703 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5869
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5869
BssSI C'ACGA_G
Cuts at: 3929 4941 3929
Size: 1012 4857
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2570 2645 794
Size: 1776 75 4018
Fragments arranged by size:
4018 1776 75
BstBI TT'CG_AA
Cuts at: 2872 2910 4673 2872
Size: 38 1763 4068
Fragments arranged by size:
4068 1763 38
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5869
BstXI CCAn_nnnn'nTGG
Cuts at: 2047 2146 2047
Size: 99 5770
BstYI r'GATC_y
Cuts at: 1972 2840 3199 3566 3951 3968 5420 5506 5518
Size: 868 359 367 385 17 1452 86 12
Cuts at: 5518 5864 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 868 385 367 359 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2130 2130
Size: 5869
BtgI C'CryG_G
Cuts at: 513 1065 2004 2065 2623 513
Size: 552 939 61 558 3759
Fragments arranged by size:
3759 939 558 552 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2545 487
Size: 683 56 1319 3811
Fragments arranged by size:
3811 1319 683 56
BtsI GCAGTG_nn'
Cuts at: 2164 3385 4179 4199 2164
Size: 1221 794 20 3834
Fragments arranged by size:
3834 1221 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5869
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5834
DraI TTT'AAA
Cuts at: 3173 3173
Size: 5869
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4876 809
Size: 507 3560 1802
Fragments arranged by size:
3560 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2304 3015 3316
Size: 54 1425 50 275 18 474 711 301
Cuts at: 3316 4205 5834 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 711 474 301 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3316 1537
Size: 275 1504 4090
Fragments arranged by size:
4090 1504 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2108 2369 2664 2825 3799 1353
Size: 737 18 261 295 161 974 3423
Fragments arranged by size:
3423 974 737 295 261 161 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2175 2351 2492 4456 4830 4976
Size: 1064 138 586 176 141 1964 374 146
Cuts at: 4976 387
Size: 1280
Fragments arranged by size:
1964 1280 1064 586 374 176 146 141
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2001 2675 2688 3060 3075 3076 3932
Size: 393 369 674 13 372 15 1 856
Cuts at: 3932 4529 5316 5584 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 856 787 674 597 393 372 369
268 15 13 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2129 2129
Size: 5869
EcoO109I rG'GnC_Cy
Cuts at: 2176 2610 2663 2728 2977 2176
Size: 434 53 65 249 5068
Fragments arranged by size:
5068 434 249 65 53
EcoRI G'AATT_C
Cuts at: 3178 3556 3178
Size: 378 5491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 5869
FspI TGC'GCA
Cuts at: 4375 4375
Size: 5869
HaeII r_GCGC'y
Cuts at: 1678 2076 5016 1678
Size: 398 2940 2531
Fragments arranged by size:
2940 2531 398
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4510 4542 4584 4616 1373
Size: 32 135 32 2938 32 42 32 2626
Fragments arranged by size:
2938 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3213 3459 669
Size: 988 281 1275 246 3079
Fragments arranged by size:
3079 1275 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5869
HpaI GTT'AAC
Cuts at: 3459 3459
Size: 5869
KasI G'GCGC_C
Cuts at: 2072 2072
Size: 5869
KpnI G_GTAC'C
Cuts at: 2892 3194 2892
Size: 302 5567
MfeI C'AATT_G
Cuts at: 3468 3468
Size: 5869
MluI A'CGCG_T
Cuts at: 5745 5745
Size: 5869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4983 5167 1305
Size: 3678 184 2007
Fragments arranged by size:
3678 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2306 3017 10
Size: 54 1425 343 474 711 2862
Fragments arranged by size:
2862 1425 711 474 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3827 4186 4345 518
Size: 648 2661 359 159 2042
Fragments arranged by size:
2661 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2067 2653 3032 3096 3285 3962 5110
Size: 418 43 586 379 64 189 677 1148
Cuts at: 5110 5355 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 586 418 379 245 189
64 43
NarI GG'CG_CC
Cuts at: 2073 2073
Size: 5869
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4378
Fragments arranged by size:
4378 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5869
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5869
NotI GC'GGCC_GC
Cuts at: 3316 3316
Size: 5869
NspI r_CATG'y
Cuts at: 1132 1229 2219 3227 4772 1132
Size: 97 990 1008 1545 2229
Fragments arranged by size:
2229 1545 1008 990 97
PasI CC'CwG_GG
Cuts at: 1348 2683 1348
Size: 1335 4534
PciI A'CATG_T
Cuts at: 4768 4768
Size: 5869
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2024 2332 1108
Size: 916 308 4645
Fragments arranged by size:
4645 916 308
PmeI GTTT'AAAC
Cuts at: 3173 3173
Size: 5869
PmlI CAC'GTG
Cuts at: 2719 2719
Size: 5869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3938 3970 5451 5483 3938
Size: 32 1481 32 4324
Fragments arranged by size:
4324 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2176 2728 2176
Size: 552 5317
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5869
PsiI TTA'TAA
Cuts at: 3439 3439
Size: 5869
PspOMI G'GGCC_C
Cuts at: 2609 2976 2609
Size: 367 5502
PstI C_TGCA'G
Cuts at: 830 2739 3108 3221 830
Size: 1909 369 113 3478
Fragments arranged by size:
3478 1909 369 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5869
PvuII CAG'CTG
Cuts at: 3096 3096
Size: 5869
SacII CC_GC'GG
Cuts at: 2068 2068
Size: 5869
SalI G'TCGA_C
Cuts at: 1936 3211 1936
Size: 1275 4594
SanDI GG'GwC_CC
Cuts at: 2176 2176
Size: 5869
SapI GCTCTTCn'nnn_
Cuts at: 2664 2664
Size: 5869
SbfI CC_TGCA'GG
Cuts at: 3108 3221 3108
Size: 113 5756
ScaI AGT'ACT
Cuts at: 4117 5829 4117
Size: 1712 4157
SfcI C'TryA_G
Cuts at: 826 1045 2735 3104 3217 4352 5033 5224 826
Size: 219 1690 369 113 1135 681 191 1471
Fragments arranged by size:
1690 1471 1135 681 369 219 191 113
SfoI GGC'GCC
Cuts at: 2074 2074
Size: 5869
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5869
SmaI CCC'GGG
Cuts at: 1872 2060 3196 1872
Size: 188 1136 4545
Fragments arranged by size:
4545 1136 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3973 4874 5136 820
Size: 197 926 2030 901 262 1553
Fragments arranged by size:
2030 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3660 493
Size: 3167 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5869
SphI G_CATG'C
Cuts at: 1132 2219 3227 1132
Size: 1087 1008 3774
Fragments arranged by size:
3774 1087 1008
SspI AAT'ATT
Cuts at: 5 52 3793 5
Size: 47 3741 2081
Fragments arranged by size:
3741 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5869
StyI C'CwwG_G
Cuts at: 513 1065 2004 2836 3308 513
Size: 552 939 832 472 3074
Fragments arranged by size:
3074 939 832 552 472
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2330 3896 3913 4066 4251 4696 325
Size: 1255 750 1566 17 153 185 445 1498
Fragments arranged by size:
1566 1498 1255 750 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4115 5827 96
Size: 274 80 33 51 1443 2138 1712 138
Fragments arranged by size:
2138 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5829 5861 5829
Size: 32 5837
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5869
XbaI T'CTAG_A
Cuts at: 3205 3205
Size: 5869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2581 2581
Size: 5869
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5869
XmaI C'CCGG_G
Cuts at: 1870 2058 3194 1870
Size: 188 1136 4545
Fragments arranged by size:
4545 1136 188
XmnI GAAnn'nnTTC
Cuts at: 3998 3998
Size: 5869
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3677 276
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI
PsrI RsrII SacI SexAI SfiI SrfI SwaI