(Linear) (Six-base) MAPSORT of: KIEE6353.seq Check: 6071 from: 1 to: 1164 KIEE6353.orf With 182 enzymes: SgfI * March 16, 2015 11:30 .. Acc65I G'GTAC_C Cuts at: 0 883 1164 Size: 883 281 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 683 1055 1070 1164 Size: 683 372 15 94 Fragments arranged by size: 683 372 94 15 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 245 1164 Size: 245 919 AlwNI CAG_nnn'CTG Cuts at: 0 19 1137 1164 Size: 19 1118 27 Fragments arranged by size: 1118 27 19 ApaI G_GGCC'C Cuts at: 0 608 975 1164 Size: 608 367 189 Fragments arranged by size: 608 367 189 ApaLI G'TGCA_C Cuts at: 0 709 1164 Size: 709 455 AvaI C'yCGr_G Cuts at: 0 53 1164 Size: 53 1111 BamHI G'GATC_C Cuts at: 0 835 1164 Size: 835 329 BanI G'GyrC_C Cuts at: 0 67 413 532 883 933 1164 Size: 67 346 119 351 50 231 Fragments arranged by size: 351 346 231 119 67 50 BanII G_rGCy'C Cuts at: 0 8 52 110 119 608 671 975 1164 Size: 8 44 58 9 489 63 304 189 Fragments arranged by size: 489 304 189 63 58 44 9 8 BbeI G_GCGC'C Cuts at: 0 71 1164 Size: 71 1093 BbsI GAAGACnn'nnnn_ Cuts at: 0 246 1164 Size: 246 918 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 514 548 1164 Size: 514 34 616 Fragments arranged by size: 616 514 34 BciVI GTATCCnnnnn_n' Cuts at: 0 912 1164 Size: 912 252 BglI GCCn_nnn'nGGC Cuts at: 0 1045 1164 Size: 1045 119 Bme1580I G_kGCm'C Cuts at: 0 608 713 831 975 1164 Size: 608 105 118 144 189 Fragments arranged by size: 608 189 144 118 105 BmrI ACTGGGnnnn_n' Cuts at: 0 1125 1164 Size: 1125 39 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 670 1071 1164 Size: 670 401 93 Fragments arranged by size: 670 401 93 BsaI GGTCTCn'nnnn_ Cuts at: 0 25 456 1164 Size: 25 431 708 Fragments arranged by size: 708 431 25 BsaAI yAC'GTr Cuts at: 0 714 1164 Size: 714 450 BsaHI Gr'CG_yC Cuts at: 0 68 1164 Size: 68 1096 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 142 667 1164 Size: 142 525 497 Fragments arranged by size: 525 497 142 BseYI C'CCAG_C Cuts at: 0 19 262 601 717 1164 Size: 19 243 339 116 447 Fragments arranged by size: 447 339 243 116 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 490 577 946 986 1077 1164 Size: 490 87 369 40 91 87 Fragments arranged by size: 490 369 91 87 87 40 BsiHKAI G_wGCw'C Cuts at: 0 713 1164 Size: 713 451 BsmBI CGTCTCn'nnnn_ Cuts at: 0 851 1164 Size: 851 313 Bsp1286I G_dGCh'C Cuts at: 0 8 52 110 119 608 671 713 831 Size: 8 44 58 9 489 63 42 118 Cuts at: 831 975 1164 Size: 144 189 Fragments arranged by size: 489 189 144 118 63 58 44 42 9 8 BspHI T'CATG_A Cuts at: 0 305 1164 Size: 305 859 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1092 1164 Size: 1092 72 BsrBI CCG'CTC Cuts at: 0 844 1164 Size: 844 320 BsrDI GCAATG_nn' Cuts at: 0 900 1164 Size: 900 264 BstAPI GCAn_nnn'nTGC Cuts at: 0 565 640 1164 Size: 565 75 524 Fragments arranged by size: 565 524 75 BstBI TT'CG_AA Cuts at: 0 867 905 1164 Size: 867 38 259 Fragments arranged by size: 867 259 38 BstXI CCAn_nnnn'nTGG Cuts at: 0 42 141 1164 Size: 42 99 1023 Fragments arranged by size: 1023 99 42 BstYI r'GATC_y Cuts at: 0 835 1164 Size: 835 329 Bsu36I CC'TnA_GG Cuts at: 0 125 1164 Size: 125 1039 BtgI C'CryG_G Cuts at: 0 60 618 1164 Size: 60 558 546 Fragments arranged by size: 558 546 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 540 1164 Size: 540 624 BtsI GCAGTG_nn' Cuts at: 0 159 1164 Size: 159 1005 EaeI y'GGCC_r Cuts at: 0 299 1010 1164 Size: 299 711 154 Fragments arranged by size: 711 299 154 EarI CTCTTCn'nnn_ Cuts at: 0 85 103 364 659 820 1164 Size: 85 18 261 295 161 344 Fragments arranged by size: 344 295 261 161 85 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 170 346 487 1164 Size: 170 176 141 677 Fragments arranged by size: 677 176 170 141 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 670 683 1055 1070 1071 1164 Size: 670 13 372 15 1 93 Fragments arranged by size: 670 372 93 15 13 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 124 1164 Size: 124 1040 EcoO109I rG'GnC_Cy Cuts at: 0 171 605 658 723 972 1164 Size: 171 434 53 65 249 192 Fragments arranged by size: 434 249 192 171 65 53 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 114 1164 Size: 114 1050 HaeII r_GCGC'y Cuts at: 0 71 1164 Size: 71 1093 KasI G'GCGC_C Cuts at: 0 67 1164 Size: 67 1097 KpnI G_GTAC'C Cuts at: 0 887 1164 Size: 887 277 MscI TGG'CCA Cuts at: 0 301 1012 1164 Size: 301 711 152 Fragments arranged by size: 711 301 152 MspA1I CmG'CkG Cuts at: 0 19 62 648 1027 1091 1164 Size: 19 43 586 379 64 73 Fragments arranged by size: 586 379 73 64 43 19 NarI GG'CG_CC Cuts at: 0 68 1164 Size: 68 1096 NspI r_CATG'y Cuts at: 0 214 1164 Size: 214 950 PasI CC'CwG_GG Cuts at: 0 678 1164 Size: 678 486 PflMI CCAn_nnn'nTGG Cuts at: 0 19 327 1164 Size: 19 308 837 Fragments arranged by size: 837 308 19 PmlI CAC'GTG Cuts at: 0 714 1164 Size: 714 450 PpuMI rG'GwC_Cy Cuts at: 0 171 723 1164 Size: 171 552 441 Fragments arranged by size: 552 441 171 PspOMI G'GGCC_C Cuts at: 0 604 971 1164 Size: 604 367 193 Fragments arranged by size: 604 367 193 PstI C_TGCA'G Cuts at: 0 734 1103 1164 Size: 734 369 61 Fragments arranged by size: 734 369 61 PvuII CAG'CTG Cuts at: 0 1091 1164 Size: 1091 73 SacII CC_GC'GG Cuts at: 0 63 1164 Size: 63 1101 SanDI GG'GwC_CC Cuts at: 0 171 1164 Size: 171 993 SapI GCTCTTCn'nnn_ Cuts at: 0 659 1164 Size: 659 505 SbfI CC_TGCA'GG Cuts at: 0 1103 1164 Size: 1103 61 SfcI C'TryA_G Cuts at: 0 730 1099 1164 Size: 730 369 65 Fragments arranged by size: 730 369 65 SfoI GGC'GCC Cuts at: 0 69 1164 Size: 69 1095 SmaI CCC'GGG Cuts at: 0 55 1164 Size: 55 1109 SphI G_CATG'C Cuts at: 0 214 1164 Size: 214 950 StyI C'CwwG_G Cuts at: 0 831 1164 Size: 831 333 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 325 1164 Size: 325 839 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 576 1164 Size: 576 588 XmaI C'CCGG_G Cuts at: 0 53 1164 Size: 53 1111 Enzymes that do cut: Acc65I AcuI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BglI Bme1580I BmrI BpmI BsaI BsaAI BsaHI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII KasI KpnI MscI MspA1I NarI NspI PasI PflMI PmlI PpuMI PspOMI PstI PvuII SacII SanDI SapI SbfI SfcI SfoI SmaI SphI StyI TaqII XcmI XmaI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BspEI BsrFI BsrGI BssHII BssSI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI MmeI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI