################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvrII BalI BamHI BbeI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BpmI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI EaeI EagI Eam1105I EclXI Eco52I EcoRI EcoT22I FauI FseI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI StuI SwaI TaqI TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccII 121 CGCG CG^CG AciI 122 GCGG CCGC(-3/-1) AocI 1177 CCTGAGG CC^T[ACGT]AGG Aor51HI 106 AGCGCT AGC^GCT AspI 720 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 918 CCCGAG C^[CT]CG[GA]G AviII 806 TGCGCA TGC^GCA BbrPI 130 CACGTG CAC^GTG BbsI 187 GAAGAC GAAGAC(2/6) BbvI 1108 GCTGC GCAGC(8/12) BclI 341 TGATCA T^GATCA BlpI 1300 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1300 GCTAAGC GC^T[ACGT]AGC BpuAI 187 GAAGAC GAAGAC(2/6) BsaAI 130 CACGTG [CT]AC^GT[GA] BsaI 522 GGTCTC GGTCTC(1/5) BsoBI 918 CCCGAG C^[CT]CG[GA]G Bsp1407I 1280 TGTACA T^GTACA BspMI 1013 ACCTGC ACCTGC(4/8) BsrDI 426 GCAATG GCAATG(2/0) BsrFI 55 GCCGGT [GA]^CCGG[CT] BsrGI 1280 TGTACA T^GTACA BstEII 1000 GGTAACC G^GT[ACGT]ACC BstPI 1000 GGTAACC G^GT[ACGT]ACC BstUI 121 CGCG CG^CG BstXI 104 CCAGCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1177 CCTGAGG CC^T[ACGT]AGG CelII 1300 GCTAAGC GC^T[ACGT]AGC Cfr10I 55 GCCGGT [GA]^CCGG[CT] DraIII 309 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 640 GACATCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 106 AGCGCT AGC^GCT Eco81I 1177 CCTGAGG CC^T[ACGT]AGG EcoNI 118 CCTCGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1000 GGTAACC G^GT[ACGT]ACC EcoRV 439 GATATC GAT^ATC FbaI 341 TGATCA T^GATCA Fnu4HI 1108 GCTGC GC^[ACGT]GC FspI 806 TGCGCA TGC^GCA HaeII 106 AGCGCT [GA]GCGC^[CT] HincII 814 GTCAAC GT[CT]^[GA]AC HindII 814 GTCAAC GT[CT]^[GA]AC ItaI 1108 GCTGC GC^[ACGT]GC MseI 164 TTAA T^TAA MslI 64 CACATGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 121 CGCG CG^CG MwoI 734 GCTCCCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 659 CATATG CA^TATG PflFI 720 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 308 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 130 CACGTG CAC^GTG PmlI 130 CACGTG CAC^GTG SacI 741 GAGCTC GAGCT^C SbfI 1129 CCTGCAGG CCTGCA^GG Sse8387I 1129 CCTGCAGG CCTGCA^GG SspBI 1280 TGTACA T^GTACA SspI 222 AATATT AAT^ATT TfiI 194 GAATC G^A[AT]TC Tru9I 164 TTAA T^TAA TseI 1108 GCTGC G^C[AT]GC Tth111I 720 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 308 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 756 GTAC GT^AC 1281 GTAC ApaI 690 GGGCCC GGGCC^C 846 GGGCCC Asp700I 365 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 950 GAAACCCTTC BanI 114 GGTGCC G^G[CT][GA]CC 321 GGCACC BcnI 688 CCGGG CC^[GC]GG 737 CCCGG BsgI 463 CTGCAC GTGCAG(16/14) 1015 CTGCAC BsmAI 523 GTCTC GTCTC(1/5) 1321 GTCTC BspLU11I 280 ACATGT A^CATGT 752 ACATGT BsrI 310 ACTGG ACTGG(1/-1) 626 CCAGT Cac8I 558 GCTTGC GC[ACGT]^[ACGT]GC 1128 GCCTGC CfoI 107 GCGC GCG^C 807 GCGC HhaI 107 GCGC GCG^C 807 GCGC HinP1I 107 GCGC G^CGC 807 GCGC NciI 688 CCGGG CC^[GC]GG 737 CCCGG NcoI 213 CCATGG C^CATGG 335 CCATGG PstI 800 CTGCAG CTGCA^G 1130 CTGCAG RsaI 756 GTAC GT^AC 1281 GTAC Tsp509I 169 AATT ^AATT 535 AATT XmnI 365 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 950 GAAACCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AflIII AluI AlwI AspHI AvaII BanII BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FokI HaeIII HapII HinfI HpaII HphI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MspI MvaI NdeII NlaIII NlaIV NspI PleI PpuMI Sau3AI Sau96I ScrFI SfaNI SfcI StyI Tsp45I TspRI XcmI XhoII