(Circular) (Six-base) MAPSORT of: pFN21AE2286.seq Check: 3661 from: 1 to: 6037 pFN21AE2286 6037 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3027 3027 Size: 6037 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3847 278 Size: 53 83 186 936 2311 2468 Fragments arranged by size: 2468 2311 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3380 1937 Size: 1443 4594 Acc65I G'GTAC_C Cuts at: 3358 3358 Size: 6037 AclI AA'CG_TT Cuts at: 1563 4164 4537 1563 Size: 2601 373 3063 Fragments arranged by size: 3063 2601 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4100 5484 5752 1632 Size: 2468 1384 268 1917 Fragments arranged by size: 2468 1917 1384 268 AfeI AGC'GCT Cuts at: 2113 2113 Size: 6037 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5840 AflIII A'CryG_T Cuts at: 1203 2134 2285 2757 4936 5913 1203 Size: 931 151 472 2179 977 1327 Fragments arranged by size: 2179 1327 977 931 472 151 AgeI A'CCGG_T Cuts at: 4868 4868 Size: 6037 AhdI GACnn_n'nnGTC Cuts at: 4766 4766 Size: 6037 AleI CACnn'nnGTG Cuts at: 1166 2073 1166 Size: 907 5130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6037 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2784 2816 2784 Size: 32 6005 AlwNI CAG_nnn'CTG Cuts at: 3780 5352 3780 Size: 1572 4465 ApaI G_GGCC'C Cuts at: 2699 2855 2699 Size: 156 5881 ApaLI G'TGCA_C Cuts at: 4094 5250 4094 Size: 1156 4881 ApoI r'AATT_y Cuts at: 1493 3346 3572 3724 5833 1493 Size: 1853 226 152 2109 1697 Fragments arranged by size: 2109 1853 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4591 160 Size: 872 3559 1606 Fragments arranged by size: 3559 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 AvaI C'yCGr_G Cuts at: 1870 1943 2923 3362 1870 Size: 73 980 439 4545 Fragments arranged by size: 4545 980 439 73 BamHI G'GATC_C Cuts at: 3367 3734 3367 Size: 367 5670 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2119 2326 3358 4813 618 Size: 325 217 627 332 207 1032 1455 1842 Fragments arranged by size: 1842 1455 1032 627 332 325 217 207 BanII G_rGCy'C Cuts at: 1391 2255 2699 2750 2788 2855 3356 1391 Size: 864 444 51 38 67 501 4072 Fragments arranged by size: 4072 864 501 444 67 51 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2199 928 Size: 971 300 4766 Fragments arranged by size: 4766 971 300 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4228 4262 1903 Size: 34 2291 34 3678 Fragments arranged by size: 3678 2291 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3929 5139 3929 Size: 1210 4827 BclI T'GATC_A Cuts at: 1548 2346 1548 Size: 798 5239 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4648 136 Size: 107 122 71 631 605 2976 1525 Fragments arranged by size: 2976 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6032 6032 Size: 6037 BlpI GC'TnA_GC Cuts at: 3306 3454 3306 Size: 148 5889 Bme1580I G_kGCm'C Cuts at: 1790 2699 2855 4098 5254 1790 Size: 909 156 1243 1156 2573 Fragments arranged by size: 2573 1243 1156 909 156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6037 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2325 2625 3329 Size: 416 53 83 399 24 908 300 704 Cuts at: 3329 3768 4726 442 Size: 439 958 1753 Fragments arranged by size: 1753 958 908 704 439 416 399 300 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6037 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2500 2500 Size: 6037 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4697 1239 Size: 3458 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4162 5027 5325 4162 Size: 865 298 4874 Fragments arranged by size: 4874 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2533 4700 882 Size: 617 1034 2167 2219 Fragments arranged by size: 2219 2167 1034 617 BsaAI yAC'GTr Cuts at: 493 1204 2137 3828 493 Size: 711 933 1691 2702 Fragments arranged by size: 2702 1691 933 711 BsaBI GATnn'nnATC Cuts at: 6031 6031 Size: 6037 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3844 4226 4861 275 Size: 53 83 186 936 2311 382 635 1451 Fragments arranged by size: 2311 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4470 4868 5142 5289 5700 4470 Size: 398 274 147 411 4807 Fragments arranged by size: 4807 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2121 2151 2291 2321 2364 Size: 30 165 30 718 30 140 30 43 Cuts at: 2364 2394 2406 2436 2706 2736 2784 2814 1178 Size: 30 12 30 270 30 48 30 4401 Fragments arranged by size: 4401 718 270 165 140 48 43 30 30 30 30 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3056 3059 3200 1186 Size: 1870 3 141 4023 Fragments arranged by size: 4023 1870 141 3 BseYI C'CCAG_C Cuts at: 2108 2330 2688 5240 5736 2108 Size: 222 358 2552 496 2409 Fragments arranged by size: 2552 2409 496 358 222 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2453 3005 1163 Size: 573 717 552 4195 Fragments arranged by size: 4195 717 573 552 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3487 4248 5276 1540 Size: 275 186 1486 761 1028 2301 Fragments arranged by size: 2301 1486 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2460 2750 3098 4098 4183 5254 2460 Size: 290 348 1000 85 1071 3243 Fragments arranged by size: 3243 1071 1000 348 290 85 BsmI GAATG_Cn' Cuts at: 3548 3641 3548 Size: 93 5944 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2255 2460 2699 2750 2788 2855 3098 Size: 399 465 205 239 51 38 67 243 Cuts at: 3098 3356 4098 4183 5254 1391 Size: 258 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 465 399 258 243 239 205 85 67 51 38 BspEI T'CCGG_A Cuts at: 5700 5700 Size: 6037 BspHI T'CATG_A Cuts at: 3926 5656 3926 Size: 1730 4307 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3027 3392 844 Size: 2183 365 3489 Fragments arranged by size: 3489 2183 365 BsrBI CCG'CTC Cuts at: 1394 1823 3924 1394 Size: 429 2101 3507 Fragments arranged by size: 3507 2101 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2438 4532 4706 65 Size: 1183 1190 2094 174 1396 Fragments arranged by size: 2094 1396 1190 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 4681 4868 1700 Size: 110 250 2621 187 2869 Fragments arranged by size: 2869 2621 250 187 110 BsrGI T'GTAC_A Cuts at: 96 3285 96 Size: 3189 2848 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6037 BssSI C'ACGA_G Cuts at: 4097 5109 4097 Size: 1012 5025 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6037 BstBI TT'CG_AA Cuts at: 4841 4841 Size: 6037 BstEII G'GTnAC_C Cuts at: 1184 3005 1184 Size: 1821 4216 BstXI CCAn_nnnn'nTGG Cuts at: 2116 2116 Size: 6037 BstYI r'GATC_y Cuts at: 1972 2481 2514 3314 3367 3734 4119 4136 5588 Size: 509 33 800 53 367 385 17 1452 Cuts at: 5588 5674 5686 6032 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 800 509 385 367 346 86 53 33 17 12 Bsu36I CC'TnA_GG Cuts at: 3183 3183 Size: 6037 BtgI C'CryG_G Cuts at: 513 1065 2218 2340 2863 513 Size: 552 1153 122 523 3687 Fragments arranged by size: 3687 1153 552 523 122 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5298 Fragments arranged by size: 5298 683 56 BtsI GCAGTG_nn' Cuts at: 2814 2933 3211 3553 4347 4367 2814 Size: 119 278 342 794 20 4484 Fragments arranged by size: 4484 794 342 278 119 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6037 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6002 DraI TTT'AAA Cuts at: 3341 3341 Size: 6037 DraIII CAC_nnn'GTG Cuts at: 2319 2319 Size: 6037 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2651 5044 809 Size: 507 1335 2393 1802 Fragments arranged by size: 2393 1802 1335 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3484 4373 6002 Size: 54 1425 50 275 18 1654 889 1629 Cuts at: 6002 8 Size: 43 Fragments arranged by size: 1654 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3484 1537 Size: 275 1672 4090 Fragments arranged by size: 4090 1672 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2148 3062 3077 3967 1353 Size: 795 914 15 890 3423 Fragments arranged by size: 3423 914 890 795 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4624 4998 5144 387 Size: 1064 138 3035 374 146 1280 Fragments arranged by size: 3035 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2748 2748 Size: 6037 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4100 4697 5484 5752 1239 Size: 393 2468 597 787 268 1524 Fragments arranged by size: 2468 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2127 2127 Size: 6037 EcoO109I rG'GnC_Cy Cuts at: 2396 2491 2577 2696 2852 2871 3036 2396 Size: 95 86 119 156 19 165 5397 Fragments arranged by size: 5397 165 156 119 95 86 19 EcoRI G'AATT_C Cuts at: 3346 3724 3346 Size: 378 5659 EcoRV GAT'ATC Cuts at: 2446 2446 Size: 6037 FspI TGC'GCA Cuts at: 2813 4543 2813 Size: 1730 4307 HaeII r_GCGC'y Cuts at: 1678 2115 5184 1678 Size: 437 3069 2531 Fragments arranged by size: 3069 2531 437 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2047 2079 2406 2438 2513 Size: 32 135 32 475 32 327 32 75 Cuts at: 2513 2545 4678 4710 4752 4784 1373 Size: 32 2133 32 42 32 2626 Fragments arranged by size: 2626 2133 475 327 135 75 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2821 3381 3627 669 Size: 988 281 883 560 246 3079 Fragments arranged by size: 3079 988 883 560 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6037 HpaI GTT'AAC Cuts at: 3627 3627 Size: 6037 KpnI G_GTAC'C Cuts at: 3362 3362 Size: 6037 MfeI C'AATT_G Cuts at: 3636 3636 Size: 6037 MluI A'CGCG_T Cuts at: 5913 5913 Size: 6037 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2488 3123 3186 5151 5335 1305 Size: 1183 635 63 1965 184 2007 Fragments arranged by size: 2007 1965 1183 635 184 63 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4215 Fragments arranged by size: 4215 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2073 3995 4354 4513 518 Size: 648 907 1922 359 159 2042 Fragments arranged by size: 2042 1922 907 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3453 4130 5278 5523 1606 Size: 1847 677 1148 245 2120 Fragments arranged by size: 2120 1847 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2218 2340 513 Size: 552 1153 122 4210 Fragments arranged by size: 4210 1153 552 122 NdeI CA'TA_TG Cuts at: 387 2665 387 Size: 2278 3759 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6037 NotI GC'GGCC_GC Cuts at: 3484 3484 Size: 6037 NspI r_CATG'y Cuts at: 1132 1229 2273 2289 2761 3395 4940 1132 Size: 97 1044 16 472 634 1545 2229 Fragments arranged by size: 2229 1545 1044 634 472 97 16 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6037 PciI A'CATG_T Cuts at: 2285 2757 4936 2285 Size: 472 2179 3386 Fragments arranged by size: 3386 2179 472 PflMI CCAn_nnn'nTGG Cuts at: 1108 2319 1108 Size: 1211 4826 PfoI T'CCnGG_A Cuts at: 2232 2741 2232 Size: 509 5528 PmeI GTTT'AAAC Cuts at: 3341 3341 Size: 6037 PmlI CAC'GTG Cuts at: 2137 2137 Size: 6037 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2784 2816 4106 4138 5619 5651 2784 Size: 32 1290 32 1481 32 3170 Fragments arranged by size: 3170 1481 1290 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2396 2491 2577 2871 3036 2396 Size: 95 86 294 165 5397 Fragments arranged by size: 5397 294 165 95 86 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6037 PsiI TTA'TAA Cuts at: 3607 3607 Size: 6037 PspOMI G'GGCC_C Cuts at: 2695 2851 2695 Size: 156 5881 PstI C_TGCA'G Cuts at: 830 2809 3139 3389 830 Size: 1979 330 250 3478 Fragments arranged by size: 3478 1979 330 250 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6037 SacI G_AGCT'C Cuts at: 2750 2750 Size: 6037 SalI G'TCGA_C Cuts at: 1936 3379 1936 Size: 1443 4594 SbfI CC_TGCA'GG Cuts at: 3139 3389 3139 Size: 250 5787 ScaI AGT'ACT Cuts at: 4285 5997 4285 Size: 1712 4325 SfcI C'TryA_G Cuts at: 826 1045 2581 2734 2805 2875 3135 3270 3385 Size: 219 1536 153 71 70 260 135 115 Cuts at: 3385 4520 5201 5392 826 Size: 1135 681 191 1471 Fragments arranged by size: 1536 1471 1135 681 260 219 191 153 135 115 71 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6037 SmaI CCC'GGG Cuts at: 1872 3364 1872 Size: 1492 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4141 5042 5304 820 Size: 197 926 2198 901 262 1553 Fragments arranged by size: 2198 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3828 493 Size: 3335 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6037 SphI G_CATG'C Cuts at: 1132 3395 1132 Size: 2263 3774 SspI AAT'ATT Cuts at: 5 52 2229 3961 5 Size: 47 2177 1732 2081 Fragments arranged by size: 2177 2081 1732 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6037 StyI C'CwwG_G Cuts at: 513 1065 2218 2340 2532 2700 3476 513 Size: 552 1153 122 192 168 776 3074 Fragments arranged by size: 3074 1153 776 552 192 168 122 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2063 2307 2316 2344 2406 2540 2876 Size: 1255 483 244 9 28 62 134 336 Cuts at: 2876 2995 4064 4081 4234 4419 4864 325 Size: 119 1069 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1069 483 445 336 244 185 153 134 119 62 28 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3285 4283 5995 Size: 274 80 33 51 1443 1308 998 1712 Cuts at: 5995 96 Size: 138 Fragments arranged by size: 1712 1443 1308 998 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2121 2153 2291 2323 2706 2738 3280 3312 5997 Size: 32 138 32 383 32 542 32 2685 Cuts at: 5997 6029 2121 Size: 32 2129 Fragments arranged by size: 2685 2129 542 383 138 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2728 1316 Size: 1412 4625 XbaI T'CTAG_A Cuts at: 3373 3373 Size: 6037 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2338 2347 2638 2011 Size: 327 9 291 5410 Fragments arranged by size: 5410 327 291 9 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6037 XmaI C'CCGG_G Cuts at: 1870 3362 1870 Size: 1492 4545 XmnI GAAnn'nnTTC Cuts at: 2374 2959 4166 2374 Size: 585 1207 4245 Fragments arranged by size: 4245 1207 585 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3845 276 Size: 53 83 186 936 2311 2468 Fragments arranged by size: 2468 2311 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI