################################## # NO CUT ENZYMES ( 203 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AfaI 120 GTAC GT^AC AluI 206 AGCT AG^CT Alw44I 212 GTGCAC G^TGCAC AlwNI 250 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 212 GTGCAC G^TGCAC AspHI 212 GTGCAC G[AT]GC[AT]^C AvaII 263 GGACC G^G[AT]CC BbsI 79 GAAGAC GAAGAC(2/6) BmyI 212 GTGCAC G[AGT]GC[ACT]^C BpuAI 79 GAAGAC GAAGAC(2/6) BsiHKAI 212 GTGCAC G[AT]GC[AT]^C BsmAI 252 GAGAC GTCTC(1/5) Bsp1286I 212 GTGCAC G[AGT]GC[ACT]^C BsrI 12 ACTGG ACTGG(1/-1) CfoI 282 GCGC GCG^C DraII 262 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO109I 262 AGGACCT [GA]G^G[ACGT]CC[CT] HaeII 281 AGCGCC [GA]GCGC^[CT] HhaI 282 GCGC GCG^C HinP1I 282 GCGC G^CGC HphI 185 TCACC GGTGA(8/7) MaeIII 97 GTGAC ^GT[ACGT]AC MboII 79 GAAGA GAAGA(8/7) PpuMI 262 AGGACCT [GA]G^G[AT]CC[CT] RsaI 120 GTAC GT^AC Tsp45I 97 GTGAC ^GT[GC]AC TspRI 191 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 220 CCAGACCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AciI 201 CCGC CCGC(-3/-1) 285 CCGC BanI 64 GGCACC G^G[CT][GA]CC 168 GGTGCC BbvCI 18 CCTCAGC CCTCAGC(-5/-2) 266 CCTCAGC BssKI 115 CCTGG ^CC[ACGT]GG 230 CCTGG BstNI 115 CCTGG CC^[AT]GG 230 CCTGG EcoRII 115 CCTGG ^CC[AT]GG 230 CCTGG MvaI 115 CCTGG CC^[AT]GG 230 CCTGG PstI 162 CTGCAG CTGCA^G 256 CTGCAG ScrFI 115 CCTGG CC^[ACGT]GG 230 CCTGG SfaNI 73 GATGC GCATC(5/9) 242 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### BbvI BsaJI BsiYI BslI BstF5I Cac8I Cfr13I DdeI Fnu4HI FokI HaeIII ItaI MnlI NlaIV Sau96I SfcI TseI