################################## # NO CUT ENZYMES ( 198 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BmyI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AciI 112 GCGG CCGC(-3/-1) AvaII 5 GGACC G^G[AT]CC BbsI 260 GAAGAC GAAGAC(2/6) BcnI 96 CCCGG CC^[GC]GG BglI 40 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 55 GCTGAGC GC^T[ACGT]AGC BpmI 64 CTCCAG CTGGAG(16/14) Bpu1102I 55 GCTGAGC GC^T[ACGT]AGC BpuAI 260 GAAGAC GAAGAC(2/6) BsmFI 146 GGGAC GGGAC(10/14) BsmI 271 GCATTC GAATGC(1/-1) BspMI 244 ACCTGC ACCTGC(4/8) BsrDI 219 CATTGC GCAATG(2/0) BstXI 26 CCATTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 55 GCTGAGC GC^T[ACGT]AGC DdeI 56 CTGAG C^T[ACGT]AG DraII 4 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 4 AGGACCC [GA]G^G[ACGT]CC[CT] HapII 97 CCGG C^CGG HindIII 153 AAGCTT A^AGCTT HinfI 263 GACTC G^A[ACGT]TC HpaII 97 CCGG C^CGG MaeII 231 ACGT A^CGT MboII 260 GAAGA GAAGA(8/7) MspA1I 110 CAGCGG C[AC]G^C[GT]G MspI 97 CCGG C^CGG NciI 96 CCCGG CC^[GC]GG PleI 263 GACTC GAGTC(4/5) PpuMI 4 AGGACCC [GA]G^G[AT]CC[CT] SbfI 42 CCTGCAGG CCTGCA^GG SfiI 39 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 177 CTCAAG C^T[CT][GA]AG Sse8387I 42 CCTGCAGG CCTGCA^GG XcmI 26 CCATTCTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AluI 75 AGCT AG^CT 154 AGCT Cac8I 45 GCAGGC GC[ACGT]^[ACGT]GC 214 GCGTGC HaeIII 39 GGCC GG^CC 48 GGCC NlaIII 143 CATG CATG^ 189 CATG PstI 43 CTGCAG CTGCA^G 89 CTGCAG SfaNI 168 GCATC GCATC(5/9) 248 GCATC SfcI 43 CTGCAG C^T[GA][CT]AG 89 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI Cfr13I EcoRII Fnu4HI FokI ItaI MnlI MvaI MwoI NlaIV Sau96I ScrFI TseI